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Search Results: 1 - 10 of 462526 matches for " Holly A. Field "
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Developments in developmental genomics
Holly A Field, Kevin P White
Genome Biology , 2003, DOI: 10.1186/gb-2003-4-11-345
Abstract: Developmental biologists from around the world converged on Boston for the SDB annual meeting to discuss topics that ranged from embryonic development in invertebrates to mammalian stem cells. Genomic approaches are driving many new discoveries in developmental biology, as was reflected in several plenary-session talks and a special workshop that featured genomic and proteomic applications. A few of the highlights are described here.The first few cell divisions in an organism's life are a complex affair. Mutational approaches in the embryo of Caenorhabditis elegans have identified genes involved in asymmetric cell division, coordinated centrosome movements and proper segregation of chromosomes. Many - in fact most - of the genes required for normal early embryonic development are only now being identified, however, thanks to high-throughput RNA interference (RNAi) screening. Fabio Piano (New York University, USA) described how large-scale functional genomics has been merged with careful developmental analysis of the events of early C. elegans embryogenesis. Piano, in parallel with other groups such as those of Julie Ahringer (The Wellcome Trust/Cancer Research UK Institute, Cambridge, UK) and Tony Hyman (Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany), has been using RNAi combined with high-resolution embryo imaging to investigate the functions of predicted C. elegans genes. Piano presented an evaluation of his own and the other groups' datasets, comparing them by the RNAi delivery technique used: soaking worms in double-stranded RNA (dsRNA), feeding them bacteria expressing dsRNA, or injecting dsRNA directly. Each group scored embryos for a standard set of phenotypes. Although there was significant overlap in the genes identified, Piano found that a large proportion of genes identified by each screen were not found by the others, indicating that multiple iterations of RNAi screening will be necessary to approach truly comprehensive c
A Reinterpretation of Historic Aquifer Tests of Two Hydraulically Fractured Wells by Application of Inverse Analysis, Derivative Analysis, and Diagnostic Plots  [PDF]
Patrick A. Hammond, Malcolm S. Field
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.65048
Abstract:

Aquifer test methods have greatly improved in recent years with the advent of inverse analysis, derivative analysis, and diagnostic plots. Updated analyses of past aquifer tests allow for improved interpretations of the data to enhance the knowledge and the predictive capabilities of the flow system. This work thoroughly reanalyzes a series of pre- and post-hydraulic fracturing, single-well aquifer tests conducted in two crystalline rock wells in New Hampshire as part of an early 1970’s study. Previous analyses of the data had relied on older manual type-curve methods for predicting the possible effects of hydraulic fracturing. This work applies inverse analysis, derivative analysis, and diagnostic plots to reanalyze the 1970’s aquifer test data. Our results demonstrate that the aquifer tests were affected by changes in flow regimes, dewatering of the aquifer and discrete fractures, and changes due to well development. Increases in transmissivities are related to well development prior to hydraulic fracturing, propagation of a single, vertical fracture hydraulically connecting the two wells after stimulation and expansion of troughs of depression. After hydraulic fracturing, the estimated total yield of the individual wells increased by 2.5 times due to the hydraulic fracturing. However, the wells may be receiving water from the same source, and well interference may affect any significant increase in their combined yield. Our analyses demonstrate the value in applying inverse analysis, derivative analysis, and diagnostic plots over the conventional method of manual type-curve analysis. In addition, our improvement in the aquifer test interpretation of the 1970’s test data has implications for more reliable estimates of sustained well yields.

Eurema Lisa in New Hampshire
James A. Field
Psyche , 1904, DOI: 10.1155/1904/53046
Abstract:
Emerging Therapies for the Treatment of Essential Tremor
Holly A. Shill
Clinical Medicine : Therapeutics , 2009,
Abstract: This article will review newer therapies for the treatment of essential tremor as well as cover potential therapies still in development. Drug pharmacology, specific dosing for ET and adverse effects will be reviewed. Finally, a rationale approach to treatment of ET will be discussed.
The Herbicide Atrazine Activates Endocrine Gene Networks via Non-Steroidal NR5A Nuclear Receptors in Fish and Mammalian Cells
Miyuki Suzawa, Holly A. Ingraham
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002117
Abstract: Atrazine (ATR) remains a widely used broadleaf herbicide in the United States despite the fact that this s-chlorotriazine has been linked to reproductive abnormalities in fish and amphibians. Here, using zebrafish we report that environmentally relevant ATR concentrations elevated zcyp19a1 expression encoding aromatase (2.2 μg/L), and increased the ratio of female to male fish (22 μg/L). ATR selectively increased zcyp19a1, a known gene target of the nuclear receptor SF-1 (NR5A1), whereas zcyp19a2, which is estrogen responsive, remained unchanged. Remarkably, in mammalian cells ATR functions in a cell-specific manner to upregulate SF-1 targets and other genes critical for steroid synthesis and reproduction, including Cyp19A1, StAR, Cyp11A1, hCG, FSTL3, LH?, INHα, αGSU, and 11?-HSD2. Our data appear to eliminate the possibility that ATR directly affects SF-1 DNA- or ligand-binding. Instead, we suggest that the stimulatory effects of ATR on the NR5A receptor subfamily (SF-1, LRH-1, and zff1d) are likely mediated by receptor phosphorylation, amplification of cAMP and PI3K signaling, and possibly an increase in the cAMP-responsive cellular kinase SGK-1, which is known to be upregulated in infertile women. Taken together, we propose that this pervasive and persistent environmental chemical alters hormone networks via convergence of NR5A activity and cAMP signaling, to potentially disrupt normal endocrine development and function in lower and higher vertebrates.
Leading the way: finding genes for neurologic disease in dogs using genome-wide mRNA sequencing
Elaine A Ostrander, Holly Beale
BMC Genetics , 2012, DOI: 10.1186/1471-2156-13-56
Abstract: Forman and colleagues apply high-throughput sequencing to a single case of canine neonatal cerebellar cortical degeneration. This implementation of whole genome mRNA sequencing, the first reported in dog, is additionally unusual due to the analysis: the data was used not to examine transcript levels or annotate genes, but as a form of target capture that revealed the sequence of transcripts of genes associated with ataxia in humans. This approach entails risks. It would fail if, for example, the relevant transcripts were not sufficiently expressed for genotyping or were not associated with ataxia in humans. But here it pays off handsomely, identifying a single frameshift mutation that segregates with the disease. This work sets the stage for similar studies that take advantage of recent advances in genomics while exploiting the historical background of dog breeds to identify disease-causing mutations.
Update on ropinirole in the treatment of Parkinson’s disease
Holly A Shill, Mark Stacy
Neuropsychiatric Disease and Treatment , 2009, DOI: http://dx.doi.org/10.2147/NDT.S3237
Abstract: ate on ropinirole in the treatment of Parkinson’s disease Review (7084) Total Article Views Authors: Holly A Shill, Mark Stacy Published Date December 2008 Volume 2009:5 Pages 33 - 36 DOI: http://dx.doi.org/10.2147/NDT.S3237 Holly A Shill, Mark Stacy Sun Health Research Institute, Sun City, AZ, USA; Duke University and Medical Center, Durham, NC, USA Abstract: Ropinirole is a dopamine agonist, approved for use to treat symptoms of early and advanced Parkinson’s disease, is now available in a 24-hour formulation in addition to the immediate release version. This review discusses the mode of action of ropinirole and compares the pharmacokinetics of both formulations. Pivotal studies leading to the approval of both preparations are reviewed in terms of efficacy, dose range and side effects. Patient factors such as compliance are discussed in terms of the place for ropinirole in the armamentarium of Parkinson’s disease therapies.
Update on ropinirole in the treatment of Parkinson’s disease
Holly A Shill,Mark Stacy
Neuropsychiatric Disease and Treatment , 2008,
Abstract: Holly A Shill, Mark StacySun Health Research Institute, Sun City, AZ, USA; Duke University and Medical Center, Durham, NC, USAAbstract: Ropinirole is a dopamine agonist, approved for use to treat symptoms of early and advanced Parkinson’s disease, is now available in a 24-hour formulation in addition to the immediate release version. This review discusses the mode of action of ropinirole and compares the pharmacokinetics of both formulations. Pivotal studies leading to the approval of both preparations are reviewed in terms of efficacy, dose range and side effects. Patient factors such as compliance are discussed in terms of the place for ropinirole in the armamentarium of Parkinson’s disease therapies.Keywords: ropinirole extended release, ropinirole immediate release Parkinson’s disease
Statistical Eclipses of Close-in Kepler Sub-Saturns
Holly A. Sheets,Drake Deming
Physics , 2014, DOI: 10.1088/0004-637X/794/2/133
Abstract: We present a method to detect small atmospheric signals in Kepler's planet candidate light curves by averaging light curves for multiple candidates with similar orbital and physical characteristics. Our statistical method allows us to measure unbiased physical properties of Kepler's planet candidates, even for candidates whose individual signal-to-noise precludes the detection of their secondary eclipse. We detect a secondary eclipse depth of 3.83 +1.10/-1.11 ppm for a group of 31 sub-Saturn (R < 6 Earth radii) planet candidates with the greatest potential for a reflected light signature ((R_p/a)^2 > 10 ppm). Including Kepler-10b in this group increases the depth to 5.08 +0.71/-0.72 ppm. For a control group with (R_p/a)^2 < 1 ppm, we find a depth of 0.36 +/- 0.37 ppm, consistent with no detection. We also analyze the light curve of Kepler-10b and find an eclipse depth of 7.08 +/- 1.06 ppm. If the eclipses are due solely to reflected light, this corresponds to a geometric albedo of 0.22 +/- 0.06 for our group of close-in sub-Saturns, 0.37 +/- 0.05 if including Kepler-10b in the group, and 0.60 +/- 0.09 for Kepler-10b alone. Including a thermal emission model does not change the geometric albedo appreciably, assuming the Bond albedo is 2/3 the geometric albedo. Our result for Kepler-10b is consistent with previous works. Our result for close-in sub-Saturns shows that Kepler-10b is unusually reflective, but our analysis is consistent with the results of Demory (2014) for super-Earths. Our results also indicate that hot Neptunes are typically more reflective than hot Jupiters.
白毛茛中生物碱的提取和hplc分析
Holly A Weber Maureen Joseph
生物工程学报 , 2004,
Abstract: 白毛茛根粉末经室温萃取后用hplc分析其中的生物碱成分,色谱柱为zorbaxeclipsexdbc18快速分离,该方法可准确测定白毛茛中主要的生物碱,包括小檗碱(berberine)和白毛莨碱(hydrastine)。萃取和hplc分析还可用于其他几种生物碱的测定,包括氢化小蘖碱(canadine)、白毛莨分碱(hydrastinine)和巴马亭(palmatine),也可以应用于其它含小檗碱的植物根。eclipsexdbc18快速分离柱采用等梯度分离,所有组分在15min内获得了高的分离度。
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