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DIVERSIDAD DE HONGOS MICORRIZóGENOS ARBUSCULARES DE UNA CRONO-SECUENCIA DE SUELOS ALUVIALES DEGRADADOS POR ACTIVIDAD MINERA EN EL BAJO CAUCA ANTIOQUE?O, COLOMBIA
Medina Sierra,Marisol; Orozco Pati?o,Francisco Hernando; Márquez Fernández,María Elena;
Revista Facultad Nacional de Agronomía, Medellín , 2009,
Abstract: arbuscular mycorrhizal fungi (amf) presence and diversity were evaluated in undisturbed and disturbed soils form alluvial mining processes. the soils belong to the tropic fluvaquent, typic dystropept, and typic paleudult sub-groups which corresponded to low, middle and high terraces, respectively, of the cauca river at taraza town. amf propagules were multiplied in leonard jars under glasshouse conditions using sterile substrate, modified hoagland's solution and different fractions of soil used as sources of inoculum, which corresponded to the size of the spores. a first assay was made in maize (zea mays) which allowed mycorrhizal colonization in roots but not spore production. in a second assay, in kudzú (pueraria phaseoloides) amf spores and colonized roots were obtained with the treatments corresponding to propagules obtained from high terrace and disturbed soil. these treatments presented a significant effect on kudzu yield (p≤0,001) respect to the other treatments. the amf spores of undisturbed and disturbed soils showed low infective capacity. nevertheless, propagules of amf were multiplied in trap cultures, which produced spores of four morphotypes. one of these was identified as g. microagregatum. the polymorphism obtained by rapd's made possible the differentiation of these morphotypes with the primer opa2. similitude above 38% was achieved using upgma system. the results indicated that four morphotypes belong to the genus glomus, but they possibly belong to different species. our results are promissory in the differentiation of native strains of amf with low number of spores collected from soil samples in rehabilitation processes, which normally is unknown.
Avances en la transformación de raíces de kudzú (Pueraria phaseoloides) y zanahoria (Daucus carota var. Danvers 126) con diferentes cepas de Agrobacterium rhizogenes para multiplicación de hongos MA Advances in transforming kudzu (Pueraria phaseoloides) and carrot (Daucus carota var. Danvers 126) roots with different Agrobacterium rhizogenes strains for increasing MA fungi growth
Medina Sierra Marisol,Orozco P. Francisco Hernando,Márquez F. María Elena
Revista Colombiana de Biotecnología , 2002,
Abstract: En el presente trabajo se transformaron raíces de kudzú (Pueraria phaseoloides) y de zanahoria (Daucus carota) en diferentes medios de cultivo, mediante el empleo de cinco cepas diferentes de Agrobacterium rhizogenes; de comportamiento diferente tanto en la transformación de zanahoria por las cepas de A. rhizogenes A.r.15834, A.r.8196 y A.r.2659; como en la transformación de kudzú por las cepas A.r.15834 y A.r.1724. Por otro lado, se logró la multiplicación en medio White modificado (WM) de las raíces transformadas de zanahoria, mientras que las de kudzú no se lograron multiplicar en ese medio ni tampoco en el medio MS modificado. Las raíces de zanahoria se emplearon para la multiplicación y esporulación del hongo micorrizógeno arbuscular (HMA) Glomus intrarradices, sin embargo las cepas nativas de HMA, aisladas de una de las zonas de minería del Bajo Cauca Antioque o, no crecieron en las raíces transformadas de esta especie ni aún en las de kudzú, a pesar de ser una planta que presenta alta afinidad por las cepas nativas de HMA. Los resultados se muestran como un avance en el procedimiento para el aislamiento de ADN y el mantenimiento de colecciones de HMA, requeridos para otras investigaciones. Kudzú (P. phaseoloides) and carrot (D. carota) roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia), did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research.
Colecistectomía videolaparoscópica II : evaluación prospectiva de los primeros 328 casos en Medellín Veideolaparoscopic cholecystectomy: experience with 328 cases in Medellín, Colombia
Carlos Hernando Morales Uribe,Juan Manuel Sierra J.,Nelson Ramírez B.,Juan G. Aristizábal
Iatreia , 1994,
Abstract: Se presenta la experiencia de los primeros 328 casos de colecistectomía laparoscópica en Medellín, realizados entre septiembre 18 de 1991 y mayo 18 de 1993, por el grupo CIGLA (cirujanos laparoscopistas de Antioquia). El grupo de pacientes estuvo formado por 252 mujeres (76.8%) y 76 hombres (23.2%) con edades comprendidas entre 14 y 85 aíios. Se intervinieron 274 casos (83.5%) como cirugía programada y 54 de urgencia por colecistitis aguda (16.5%). Fue necesario convertir el procedimiento a cirugía abierta en 5 pacientes (1.5%); dos de ellos tenían adherencias firmes, anatomía confusa y fístulas colecistoduodenales; otros dos presentaban inflamación aguda y marcado edema y el último sufrió una lesión iatrogénica de las vías biliares; sólo se presentaron 6 casos de complicación mayor (1.8%): una colección sub hepática infectada que requirió drenaje quirúrgico, una lesión del conducto hepático derecho, dos casos de litiasis residual y dos de pancreatitis postoperatoria. El tiempo quirúrgico fue en promedio 46.6 minutos en los casos electivos y 63.3 en los urgentes. La hospitalización duró menos de un día en 280 pacientes (85.3%). Se concluyó que, también en nuestro medio, la colecistectomía videolaparoscópica es el tratamiento de elección de la litiasis vesicular. We report on our experience with 328 cases of laparoscopic cholecystectomy performed between September 18, 1991 and May 18, 1993 by a specialized surgical team in Medellín, Colombia. The patients were 252 women (76.8%) and 76 men (23.2%), with ages between 14 and 85 years. The surgical procedure was elective in 274 of them (83.5%) while 54 (16.5%) were emergencies due to acute cholecystitis. In 5 cases (1.5%) it became necessary to convert the procedure to open surgery: 2 of them had strong adherences, obscure anatomy and cholecystoduodenal fistula; in two there was acute inflammation and marked edema and the last one suffered an iatrogenic lesion of the biliary ducts. There were only six cases of major complications (1.8%): infected subhepatic collection that required surgical drainage and lesion of the right hepatic duct, (one case each); residuallithiasis and postoperative pancreatitis (2 cases each). Average surgical time was 46.6 minutes in the elective cases and 63.3 in the ur. gent ones. Hospital stay lasted less than one day in 280 patients (85.3%). We conclude that in our center, as in other places, videolaparoscopic cholecystectomy is the treatment of choice for gallbladder lithiasis.
Colecistectomía videolaparóscopica: cirugía de invasión mínima Videolaparoscopic cholecystectomy: minimal invasion surgery
Carlos Hernando Morales Uribe,Juan Manuel Sierra J.,Nelson Ramírez B.
Iatreia , 1994,
Abstract: El desarrollo de la colecistectomía videolaparoscópica constituye uno de los avances quirúrgicos más importantes de los últimos tiempos y abre nuevos horizontes en el tratamiento de las enfermedades torácicas y abdominales. Se presenta un breve recuento histórico del desarrollo de esta técnica, la forma de practicarla, sus indicaciones, complicaciones y beneficios. The development of laparoscopic cholecystectomy is one of the most important surgical advances of recent years; it opens new horizons for the treatment of thoracic and abdominal diseases. The technique and a historical account of its development are briefly described; its indications, complications and benefits are summarized.
DIVERSIDAD DE HONGOS MICORRIZóGENOS ARBUSCULARES DE UNA CRONO-SECUENCIA DE SUELOS ALUVIALES DEGRADADOS POR ACTIVIDAD MINERA EN EL BAJO CAUCA ANTIOQUE O, COLOMBIA DIVERSITY OF ARBUSCULAR MYCORRHIZAL FUNGI IN A CHRONO-SEQUENCE OF ALLUVIAL AND DEGRADED SOILS DUE TO MINING PROCESSES IN BAJO CAUCA ANTIOQUE O, COLOMBIA
Marisol Medina Sierra,Francisco Hernando Orozco Pati?o,María Elena Márquez Fernández
Revista Facultad Nacional de Agronomía, Medellín , 2009,
Abstract: Se evaluó la presencia de hongos micorrizógenos arbusculares (HMA) en suelos de zonas alteradas y no alteradas por minería de aluvión de los subgrupos Tropic Fluvaquent, Typic Dystropept y Typic Paleudult de terrazas Baja, Media, y Alta, respectivamente, del río Cauca a la altura del municipio de Tarazá (Antioquia, Colombia). La multiplicación de propágulos de HMA se realizó en jarras de Leonard en invernadero, utilizando sustrato estéril, solución Hoagland's modificada y como inóculo diferentes fracciones de suelo correspondientes al tama o de las esporas. Se realizó un primer ensayo en maíz (Zea mays) en el cual se logró colonización de las raíces pero no esporulación; en un segundo ensayo en kudzú (Pueraria phaseoloides) se logró colonización y esporulación en los tratamientos provenientes de suelo de terraza alta y suelo disturbado y efecto significativo en el rendimiento del kudzú (P≤0,001) respecto a los demás tratamientos. Las esporas de suelos disturbados y no disturbados por minería de aluvión en el Bajo Cauca antioque o (Colombia) mostraron baja capacidad infectiva; sin embargo, en cultivos trampa fue posible multiplicar HMA que produjeron esporas de cuatro morfotipos diferentes, uno de los cuales se identificó como G. microagregatum. El polimorfismo de los morfotipos obtenidos mediante la técnica de RAPD's permitió diferenciarlos con el cebador OPA2 y el agrupamiento por UPGMA con todos los cebadores mostró similitud mayor al 38% entre ellos. Los resultados moleculares y morfológicos, permitieron ubicar los cuatro morfotipos en el género Glomus pero posiblemente pertenecen a especies diferentes. Los resultados obtenidos son promisorios en la diferenciación de cepas nativas con bajo número de esporas colectadas a partir de muestras de suelos en proceso de rehabilitación, de los cuales no se conoce su composición de HMA. Arbuscular mycorrhizal fungi (AMF) presence and diversity were evaluated in undisturbed and disturbed soils form alluvial mining processes. The soils belong to the Tropic Fluvaquent, Typic Dystropept, and Typic Paleudult sub-groups which corresponded to Low, Middle and High terraces, respectively, of the Cauca river at Taraza town. AMF propagules were multiplied in Leonard jars under glasshouse conditions using sterile substrate, modified Hoagland's solution and different fractions of soil used as sources of inoculum, which corresponded to the size of the spores. A first assay was made in maize (Zea mays) which allowed mycorrhizal colonization in roots but not spore production. In a second assay, in kudzú (Pueraria phaseoloides)
IMT504: A New and Potent Adjuvant for Rabies Vaccines Permitting Significant Dose Sparing  [PDF]
Alejandro D. Montaner, Analia De Nichilo, Juan M. Rodriguez, Andres Hernando-Insua, Juan Fló, Ricardo A. Lopez, Verónica Sierra, Claudio Paolazzi, Oscar Larghi, David L. Horn, Jorge Zorzopulos, Fernanda Elias
World Journal of Vaccines (WJV) , 2012, DOI: 10.4236/wjv.2012.24025
Abstract: Background: Rabies virus infection causes encephalitis, which is almost always fatal. Vaccination can be extremely effective at preventing disease but is prohibitively costly. Vaccine formulations allowing dose-sparing and fewer inoculations with faster antibody response would be extremely desirable. IMT504, an immunostimulatory non-CpG oligo-deoxynucleotide, is a highly potent vaccine adjuvant. Methods: Human and rat antibody measurements, and rat chal-lenge studies were performed. Results: In rats, highly effective immune responses with IMT504 were observed even after diluting vaccine up to 1/625. In highly lethal, live intracerebral rabies challenge studies, protection occurred even with extremely dilute vaccine plus IMT504. In humans, antibody titers developed faster and were significantly higher with IMT504-adjuvanted diluted vaccine vs non-adjuvanted vaccine (full strength or diluted). All five administered IMT504-adjuvanted diluted vaccine reached protective antibodies (≥0.5 IU/ml) after the second injection. After the third injection, individuals receiving IMT504-adjuvanted diluted vaccine reached levels approximately 10 times higher than controls (M ± SEM: 31.0 ± 10.9 vs 3.40 ± 0.99 IU/ml). Conclusions: These data suggest that IMT504 may allow fewer inoculations, highly significant dose-sparing of vaccine, rapid antibody production and protection from rabies. Extensive clinical studies are necessary to confirm if the use of IMT504 will permit significantly greater access to highly effective life-saving rabies vaccines.
Incidencia y Evitabilidad de Eventos Adversos en Pacientes Hospitalizados en tres Instituciones Hospitalarias en Colombia, 2006
Gaitán-Duarte,Hernando; Eslava-Schmalbach,Javier; Rodríguez-Malagon,Nelcy; Forero-Supelano,Víctor; Santofimio-Sierra,Dagoberto; Altahona,Hernando; ,;
Revista de Salud Pública , 2008, DOI: 10.1590/S0124-00642008000200002
Abstract: objective determining adverse event (ae) incidence, preventability, classification and impact for establishing their importance as a public health problem within the colombian social security system. methodology this was a study of a prospective inpatient cohort from three colombian general-practice institutions. inclusion criteria: at least 12 hours' length of hospital stay during 2006. exclusion criteria: suffering psychiatric disorders and ae which had occurred before hospitalisation indexing. the sample consisted of 6 557 patients. source of information: clinical charts. being a three-phase design, the first phase consisted of translating and standardising screening and causation formats, phase ii of actively monitoring screening criteria and phase iii of evaluating causation regarding the care being provided, based on specialist committee concept on a 0-6 scale. the variables measured were age, gender, social security affiliation, cumulative ae incidence, temporality, preventability of ae and disability resulting from them. results 6 688 patients were evaluated; 505 of them fulfilled positive screening criteria (95 % ci=7,9;7,3-8,6), 310 presented at least one ae during their hospitalisation (95 %ci for accumulated incidence=4,6; 4,1-5,1). ae were considered to have been preventable in 189 cases (95 % ci=61;55-66) and permanent disability occurred in 1,3 % of them. ae-associated mortality was 6,4 % (20/310). hospitalisation became increased to 1 072 days as a direct consequence of ae. conclusions this study revealed an important incidence of ae in three colombian hospitals, these being mainly preventable. their ongoing monitoring as a part of risk management systems could reduce costs and ae-associated morbidity and mortality.
Trade, Technology, Income Distribution and Growth  [PDF]
Hernando Zuleta, Luiza Pogorelova
Theoretical Economics Letters (TEL) , 2014, DOI: 10.4236/tel.2014.46061
Abstract:

The standard Hecksher-Ohlin model predicts that trade liberalization leads to a decline in the rate of return of the scarce factor of production. However, the empirical evidence of the falling labor share in some developing countries contrasts with the theory. We show that if a simple change in technology is introduced into the standard model, conditions exist for the rate of return of the scarce factor of production to increase. In particular, the price of the exported good and the amount of capital the country owns can serve as determinants whether the rate of return of the abundant factor will increase.

MERCANTILISMO,ACUMULACIóN DE CAPITAL Y DESARROLLO ECONóMICO EN LA ECONOMíA MONETARIA DE PRODUCCIóN (NACIONAL)
Matallana,Hernando;
Cuadernos de Economía , 2011,
Abstract: the central thesis of the essay is that a successful process of national capitalist development in a world market characterized by the existence of multiple national currencies and divided in the hegemonic developed countries of the first world and the underdeveloped dependent countries of the third world (and the second world) requires a mercantilist strategy of economic development in conformity with the monetary logic of capitalism. the thesis rests on the context of the heterodox monetary-keynesian conception of capitalism in so far as a monetary production economy.
Comunicación, participación y democracia1
Rojas,Hernando;
Universitas Humanística , 2006,
Abstract: this paper empirically explores the role of media and political conversation as antecedents to political engagement, a set of political attitudes and behaviors. political engagement was operationalized as political interest, political knowledge, political efficacy, associational membership, and civic and political participation. the relationships of conversation, news and entertainment media uses were examined in the context of a tumultuous society. statistical analyses suggest that the consumption of news media and political conversation are positively related to all aspects of political engagement. on the other hand, certain entertainment media uses are negatively related. the relevance of media use and political conversation to explain political engagement is discussed and their potential to predict support for democracy is explored.
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