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Search Results: 1 - 10 of 8797 matches for " Helen Silveira Coimbra "
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Comparación de distintas extracciones hidroalcohólicas de plantas con indicativo etnográfico antiséptico/desinfectante
Voigt Mota,Fernanda; Lambrecht Gon?alves,Carolina; Damé Schuch,Luiz Filipe; Silveira Coimbra,Helen; Hartwig,Carla;
Revista Cubana de Plantas Medicinales , 2011,
Abstract: introduction: the use of low risk natural products in the animal production has acquired growing importance in the last few years. objectives: to compare the antibacterial action of several hydroalcoholic extracts from 4 plants (baccharis trimera [less] dc, bidens pilosa l., eucalyptus sp. and tagetes minuta l.) against 2 microorganisms causing mastitis (staphylococcus aureus and streptococcus agalactia). methods: extracts from fresh leaves using 92.8°alcohol and from dry leaves using 70° and 50° alcohol were obtained. the antibacterial action of each extract was measured on the basis of the capacity of inactivating 105-106 cfu/ml of each bacterium at 3 contacting times of 30 s, 5 min and 20 min. the turkev′s assay-based variation and comparison analysis of the means provided the final outcomes. results: all the extracts had some antibacterial effect. the extract from t. minuta dry leaves using 70° alcohol showed the biggest effect (p< 0,05) whereas the extract from eucalyptus spp. fresh leaves proved to be more effective, being the difference significant. the other 2 plants did not show any difference in terms of antibacterial effect for the various forms of obtaining the extract. conclusions: the antibacterial action is greater with dry leaf extract from t. minuta and fresh leaf extract from eucalyptus spp.
Comparación de distintas extracciones hidroalcohólicas de plantas con indicativo etnográfico antiséptico/desinfectante Comparison of several hydroalcoholic extracts from plants with antiseptic/disinfectant ethnographic indication
Fernanda Voigt Mota,Carolina Lambrecht Gon?alves,Luiz Filipe Damé Schuch,Helen Silveira Coimbra
Revista Cubana de Plantas Medicinales , 2011,
Abstract: Introducción: la utilización de productos naturales y de bajo riesgo en la producción animal ha alcanzado elevada importancia en los últimos a os. Objetivos: comparar la actividad antibacteriana de diferentes extractos hidroalcohólicos de 4 plantas (Baccharis trimera [Less] DC, Bidens pilosa L., Eucalyptus sp. y Tagetes minuta L.) frente a 2 microorganismos que causan mastitis (Staphylococcus aureus y Streptococcus agalactia). Métodos: se obtuvieron extractos de hojas frescas con alcohol a 92,8° y de hojas secas con alcohol a 70 y 50°. La actividad antibacteriana de cada extracto se midió por la capacidad de inactivación de 10(5)-10(6) UFC/mL de cada bacteria, en 3 tiempos de contacto, 30 s, 5 y 20 min. Los resultados obtenidos se hicieron por análisis de variación y comparación de las medias utilizando el ensayo de Turkey. Resultados: se obtuvo efecto antibacteriano con los extractos de todas las plantas. Para T. minuta, el extracto de hoja seca con alcohol de 70° fue el efecto mayor (p< 0,05), mientras que para Eucalyptus spp., se encontró que el extracto de hoja fresca resultó más eficaz, con diferencia significativa. Las otras 2 plantas no presentaron diferencia de efecto antibacteriano para las distintas formas de obtención de extracto. Conclusiones: hay mayor actividad antibacteriana, cuando el extracto de T. minuta es obtenido de hojas secas y en Eucalyptus spp. de hojas frescas. Introduction: the use of low risk natural products in the animal production has acquired growing importance in the last few years. Objectives: to compare the antibacterial action of several hydroalcoholic extracts from 4 plants (Baccharis trimera [Less] DC, Bidens pilosa L., Eucalyptus sp. and Tagetes minuta L.) against 2 microorganisms causing mastitis (Staphylococcus aureus and Streptococcus agalactia). Methods: extracts from fresh leaves using 92.8°alcohol and from dry leaves using 70° and 50° alcohol were obtained. The antibacterial action of each extract was measured on the basis of the capacity of inactivating 10(5)-10(6) CFU/ml of each bacterium at 3 contacting times of 30 s, 5 min and 20 min. The Turkev′s assay-based variation and comparison analysis of the means provided the final outcomes. Results: all the extracts had some antibacterial effect. The extract from T. minuta dry leaves using 70° alcohol showed the biggest effect (p< 0,05) whereas the extract from Eucalyptus spp. fresh leaves proved to be more effective, being the difference significant. The other 2 plants did not show any difference in terms of antibacterial effect for the various forms of obtaini
ANTIBACTERIAL ACTIVITY KINETICS OF MEDICINAL PLANTS EXTRACTS AGAINST CINéTICA DA ATIVIDADE ANTIBACTERIANA IN VITRO DE EXTRATOS NATURAIS FRENTE A MICRORGANISMOS RELACIONADOS à MASTITE BOVINA
Luiz Filipe Dame Schuch,José Maria Wiest,Helen Silveira Coimbra,Luciana Souza Prestes
Ciência Animal Brasileira , 2008,
Abstract: The subject of this paper is to test antimicrobials activities by medicinal plants extracts against more important contagious bovine mastitis pathogens. Disinfectants solutions was made from Baccharis trimera (Less) D.C., Compositae (Asteracea), Eucalyptus spp Labill., Myrtaceae e Tagetes minuta (Linn.), Compositae (Asteracea) plants by hidroalcoholic extraction (EHA) or decoction (DEC). S. aureus, S. agalactiae, and P. aeruginosa were used. To test for in vitro efficacy, each solution disinfectant was mixed with bacterial suspension containing 105 CFU.mL-1, by 30 seconds, two, 10 ant 30 minutes, with and without 20% of integral milk. Viable bacteria were evaluated by directed plating of neutralized aliquots. The worked included chlorhexidine 0,18% by control and it was executed in duplicate. EHA Eucalytpus spp and EHA T. minuta were as effective as control chlorhexidine against S. aureus. This solutions plus EHA B. trimera, were as effective as control against S. agalactiae. DEC Eucalyptus and DEC B. trimera also inactivated S. agalactiae in more prolongated time. Chlorhexidine was the best against P. multocida in milk absence, although the EHA were effective at ten or thirty minutes. All solutions, inclusive control, it was sensibility to organic load. The observations from the in vitro studies presented here need to be substantiated by in vivo studies by to confirm the potentiality use of plants medicinal extracts as disinfectants/antisepsis in livestock health. O presente trabalho busca avaliar a cinética da atividade antimicrobiana de extratos de plantas medicinais frente a bactérias relacionadas com mastite bovina. Para tal, foram produzidas solu es desinfetantes a partir de folhas e talos de Baccharis trimera (Less) D.C., Compositae (Asteraceae), Eucalyptus spp Labill., Myrtaceae e Tagetes minuta (Linn.), Compositae (Asteraceae), através de extra o hidroalcoólica (EHA) e decocto (DEC). Os microrganismos utilizados foram S. aureus, S. agalactiae e P.aeruginosa. Avalia o da atividade antimicrobiana foi realizada permitindo o contato da solu o desinfetante com uma suspens o bacteriana com a concentra o de ao menos 105 UFC.mL-1 de cada, por intervalos de 30 segundos, 2, 10 e 30 minutos, com e sem matéria organica. Após, alíquotas foram semeadas em placas de ágar BHI e o número de col nias remanescentes foi contado. O trabalho foi realizado com um controle comercial, clorexidina a 0,18%, e sempre em duplicata. Encontrou-se que para S. aureus, os EHA de Eucalyptus spp e de T. minuta n o diferiram do controle, para S. agalactiae além daquelas duas,
Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR = Heterogeneidade molecular da Malassezia pachydermatis através de RAPD-PCR
Patrícia da Silva Nascente,Helen Silveira Coimbra,Ana Raquel Mano Meinerz,Mário Carlos Araújo Meireles
Acta Scientiarum : Biological Sciences , 2010,
Abstract: Several methodologies in molecular biology have been used in theinvestigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiologicalstudy of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes. Várias metodologias em biologia molecular têm sido aplicadas para estudar a M. pachydermatis diferenciando-a em subgrupos. Recentemente utiliza-se a investiga o molecular desta espécie isolada de otite externa e dermatite, e também de isolados da mesma de animais hígidos, para um estudo epidemiológico da levedura. O objetivo deste trabalho foi identificar diferen as moleculares entre isolados de M. pachydermatis obtidos de casos de otite externa canina. Para isto, amostras da levedura provenientes de c es com esta enfermidade foram estudadas através da técnica de Polimorfismo de DNA Amplificado aoAcaso - Rea o da Polimerase em Cadeia (RAPD–PCR) para pesquisa de heterogeneidade molecular. A extra o de DNA foi realizada no processo fenol-cloroformio e a técnica de RAPD foi estudada com o primer AGAATCCGCC. Pode-se observar com esta metodologia, varia o no número e posi o das bandas entre os 49 isolados estudados, podendo-se agrupá-los em nove padr es. Os agrupamentos formados pelos isolados n o apresentaram rela o com ra a, idade ou sexo do animal. Concluindo-se que a M. pachydermatis apresenta diferen as em seu perfil molecular, observado pela técnica molecular (RAPD-PCR) que permite classificar os isolados desta espécie em até nove subtipos.
O desenvolvimento da consciência fonológica em crian as com Síndrome de Down pode facilitar a alfabetiza o e contribuir para a inclus o no ensino regular? Can the development of phonological awareness in children with Down Syndrome facilitate literacy and contribute to the inclusion in mainstream education?
Cinthia Coimbra de Azevedo,Cacilda Silveira Pinto,Leonor Bezerra Guerra
Revista CEFAC , 2012,
Abstract: As leis educacionais asseguram o direito das crian as ao ensino fundamental a partir dos seis anos, propiciando a elas a exposi o antecipada a estratégias pedagógicas que visam à alfabetiza o. A inclus o escolar de crian as com Síndrome de Down (SD) acontece já na educa o infantil e se torna cada vez mais frequente, possibilitando oportunidades ímpares para o desenvolvimento cognitivo dessas, o que inclui a alfabetiza o. A diversidade de aprendizes com necessidades educacionais individuais, sem o devido apoio de uma equipe interdisciplinar, dificulta a aquisi o da leitura e escrita de forma igualitária. O desconhecimento, por parte dos educadores, dos pré-requisitos cognitivos mínimos necessários à alfabetiza o, também contribui para o seu insucesso. Um desses pré-requisitos é a consciência fonológica, pouco trabalhada intencionalmente já na educa o infantil. Estratégias que visam orientar educadores no desenvolvimento objetivo da consciência fonológica em crian as com SD podem facilitar a alfabetiza o e contribuir para a inclus o escolar. Ou será possível incluir pedagogicamente essa popula o sem que ocorram adapta es curriculares e um trabalho interdisciplinar efetivo? There are laws that ensure the educational rights for children to basic education since they are six years-old, providing early exposure to teaching strategies aiming literacy. Educational inclusion of children with Down syndrome (DS) occurs in early childhood education and has become increasingly common, providing unique opportunities for cognitive development, including literacy. The diversity of learners with individual educational needs, without proper support from an interdisciplinary team, hinders the acquisition of reading and writing equally. The educators' misunderstanding about minimum prerequisites necessary for literacy also contributes to their failure. One of these prerequisites is phonological awareness, ability that usually is not intentionally developed in early childhood education. Strategies that aim to guide educators in the objective development of phonological awareness in children with Down syndrome may contribute to the educational inclusion. Or would it be possible to educationally include this population without any curricular adaptations or an interdisciplinary effective work?
Aspectos biológicos de Argyrotaenia sphaleropa (Meyrick 1909) (Lepidoptera: Tortricidae) em dietas artificiais com diferentes fontes proteicas
Manfredi-Coimbra, Silvana;Garcia, Mauro Silveira;Loeck, Alci Enimar;Botton, Marcos;Foresti, Josemar;
Ciência Rural , 2005, DOI: 10.1590/S0103-84782005000200002
Abstract: biology aspects of argyrotaenia sphaleropa meyrick fed on artificial diets with different protein sources were studied: d1-white bean, wheat germ, soybean protein and casein; d2-common bean and yeast and d3-common bean, yeast and wheat germ, evaluating the duration and viability of all developmental stages (egg, larval, prepupa and pupa) and of the total cycle (egg-adult), sex ratio, pupa weight, fecundity, longevity and life table of fertility. tests were conducted in the laboratory at 25±1°c, 65±10% rh and 14h of photophase. duration of the egg stage was 6.6 days on all diets. the longest duration of larval and prepupal stages on d1 and pupal stages on d2, resulting in a longer duration of the total cycle on these two diets (30,9 and 30,8 days). the total viability was higher than 62% on all diets, and there was no statistical difference among the treatments. the number of instars was four or five on all treatments. the lowest fecundity was observed in d1. based on the fertility life table, d3 was the most suitable diet for rearing a. sphaleropa, due to the lowest development time (t), the highest finite increasing rate (l), and total viability exceeding 75%.
Promo??o em saúde mental: a enfermagem criando e intervindo com histórias infantis
Braga, Gimene Cardozo;Silveira, Esalba Maria;Coimbra, Valéria Cristina Christello;Porto, Adrize Rutz;
Revista Gaúcha de Enfermagem , 2011, DOI: 10.1590/S1983-14472011000100016
Abstract: this paper refers to the importance of the childish stories as an instrument, for the nursing action and the mental health promotion in childhood. the objective was to verify the recognition of the emotions of children, through stories specific to health. this is a descriptive-exploratory study by qualitative nature, conducted in seven groups meetings with six children aged seven to ten years in an outpatient childish mental health. in the data collection were used as instruments: six fairy tales, the play of emotions, emotional faces, drawings, paintings and clay sculptures. it was used thematic analysis, in which emerged: the recognition of emotions. the children recognized four basics emotions: sadness, joy, fear and anger. it was found in the study that the creation of contextualized stories and the strategy of storytelling group may be useful as a tool for research and qualification of nursing actions, to promote children's mental health.
Effect of different salt concentrations on the reduction of bean (Phaseolus vulgaris) cooking time
Juliano Garcia Bertoldo,Jefferson Luís Meirelles Coimbra,Cristian Berto da Silveira,Diego Toaldo
Biotemas , 2008,
Abstract: The purpose of this work was to examine the effect of different saline concentrations on the cooking time of aged bean seeds. This experiment was conducted at the Molecular Genetics and Improvement Institute (IMEGEM / CAV), employing random block design with two repetitions, in a 2 x 4 factorial arrangement (two cultivars and four doses of NaCl). Two cultivars were tested, one belonging to the carioca group (Pérola) and the other belonging to the black group (Uirapuru). After weighing, the grains were imbibed in different sodium chloride (NaCl) concentrations (0g, 5g, 10g and 25g) for 12 hours at 25°C. Subsequently, these grains were submitted to a cooking test, using the Mattson cooking apparatus. It was then possible to determine the NaCl concentrations in which the cooking time presented a significant reduction. The Pérola cultivar cooking time was reduced by 31 minutes by the addition of 11g of NaCl and the Uirapuru cooking time was reduced by 25 minutes by the addition of 16.75g of NaCl.
Aspectos biológicos de Argyrotaenia sphaleropa (Meyrick 1909) (Lepidoptera: Tortricidae) em dietas artificiais com diferentes fontes proteicas
Manfredi-Coimbra Silvana,Garcia Mauro Silveira,Loeck Alci Enimar,Botton Marcos
Ciência Rural , 2005,
Abstract:
Osteoprotegerin Secretion by Mevastatin via p38MAPK and NF-kB  [PDF]
Helen Smith
Open Journal of Rheumatology and Autoimmune Diseases (OJRA) , 2012, DOI: 10.4236/ojra.2012.22006
Abstract: Osteoprotegerin (OPG) is a protein produced by many cell types that has the remarkable property of inhibiting bone loss. It does this by binding to the key bone resorptive cytokine, receptor activator of NF-kB ligand (RANKL). This cytokine is produced mainly by osteoblastic cells and is instrumental in osteoclast differentiation. If the ratio of RANKL:OPG increases, bone resorption increases and results in bone loss in diseases such osteoporosis, rheumatoid arthritis and hypercalcaemia of malignancy. Hence, if drugs can be found that increase OPG, this will decrease the activity of osteoclasts and therefore bone resorption. Statins are cholesterol lowering drugs that have recently been shown to increase bone formation in rodents. It was hypothesised from this finding that this could be due to an increase in OPG production. If these commonly prescribed drugs could be used to prevent bone loss or to increase bone formation then this may prove a useful means of reducing fracture risk in patients. Treating Saos-2 osteoblast-like cells in vitro with mevastatin increased OPG production and secretion through the mevalonate pathway. A failure of geranylgeranylation of Rho and/or farnesylation of Ras proteins leads to an increase in PI-3K activation then AKT activation leading to several different signaling pathways such as MAPK’s and NF-kB. NF-kB and p38MAPK inhibitors prevented the statin stimulation of OPG but not the decrease in cell number, suggesting that statins regulate OPG secretion via PI-3K, p38MAPK and NF-kB.
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