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Search Results: 1 - 10 of 30163 matches for " Hee-Jeong Jin "
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Genetic Approach to Elucidation of Sasang Constitutional Medicine
Bu-Yeo Kim,Seongwon Cha,Hee-Jeong Jin,Sangkyun Jeong
Evidence-Based Complementary and Alternative Medicine , 2009, DOI: 10.1093/ecam/nep058
Abstract: Sasang Constitutional Medicine (SCM) offers a medical principle that classifies humans into four constitution groups and guides their treatment with constitution-matched medical assistance. The principle of this traditional medicine, although requires significant scientific support, appears to suggest a genetic influence on constitution type. The relative frequency of constitution types in a population, for instance, has remained relatively constant since Jema Lee first described them from his observations. In addition, the body compartment concept of SCM appears to be related to the anterio–posterior patterning of the embryonic gut and associated internal organs. This study describes the attributes of the constitution concept of SCM that can be interpreted in the language of genetics and current approaches to identity the genetic factors that make up the constitution. These efforts should make it possible to interpret the principle of this traditional medicine scientifically. Considering the recent trend in medicine that pursues individualized or tailored medical offerings, once SCM is proven to be explainable with scientific evidence, it will be able to contribute to and take a place in the rapidly evolving medicine environment.
Extended -Regular Sequence for Automated Analysis of Microarray Images
Jin Hee-Jeong,Chun Bong-Kyung,Cho Hwan-Gue
EURASIP Journal on Advances in Signal Processing , 2006,
Abstract: Microarray study enables us to obtain hundreds of thousands of expressions of genes or genotypes at once, and it is an indispensable technology for genome research. The first step is the analysis of scanned microarray images. This is the most important procedure for obtaining biologically reliable data. Currently most microarray image processing systems require burdensome manual block/spot indexing work. Since the amount of experimental data is increasing very quickly, automated microarray image analysis software becomes important. In this paper, we propose two automated methods for analyzing microarray images. First, we propose the extended -regular sequence to index blocks and spots, which enables a novel automatic gridding procedure. Second, we provide a methodology, hierarchical metagrid alignment, to allow reliable and efficient batch processing for a set of microarray images. Experimental results show that the proposed methods are more reliable and convenient than the commercial tools.
Development of Self-Compatible B. rapa by RNAi-Mediated S Locus Gene Silencing
Hee-Jeong Jung, Hyo-Jin Jung, Nasar Uddin Ahmed, Jong-In Park, Kwon-Kyoo Kang, Yoonkang Hur, Yong-Pyo Lim, Ill-Sup Nou
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0049497
Abstract: The self-incompatibility (SI) system is genetically controlled by a single polymorphic locus known as the S-locus in the Brassicaceae. Pollen rejection occurs when the stigma and pollen share the same S-haplotype. Recognition of S-haplotype specificity has recently been shown to involve at least two S-locus genes, S-receptor kinase (SRK) and S-locus protein 11 or S locus Cysteine-rich (SP11/SCR) protein. Here, we examined the function of S60, one SP11/SCR allele of B. rapa cv. Osome, using a RNAi-mediated gene silencing approach. The transgenic RNAi lines were highly self-compatible, and this trait was stable in subsequent generations, even after crossing with other commercial lines. These findings also suggested that the resultant self-compatibility could be transferred to commercial cultivars with the desired performances in B. rapa.
Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model
James L. Henry,Kiran Yashpal,Howard Vernon,Jaesung Kim,Hee-Jeong Im
Pain Research and Treatment , 2012, DOI: 10.1155/2012/127636
Abstract: Objective. To develop a novel animal model of persisting lumbar facet joint pain. Methods. Sprague Dawley rats were anaesthetized and the right lumbar (L5/L6) facet joint was exposed and compressed to ~1?mm with modified clamps applied for three minutes; sham-operated and na?ve animals were used as control groups. After five days, animals were tested for hind-paw sensitivity using von Frey filaments and axial deep tissue sensitivity by algometer on assigned days up to 28 days. Animals were sacrificed at selected times for histological and biochemical analysis. Results. Histological sections revealed site-specific loss of cartilage in model animals only. Tactile hypersensitivity was observed for the ipsi- and contralateral paws lasting 28 days. The threshold at which deep tissue pressure just elicited vocalization was obtained at three lumbar levels; sensitivity at . Biochemical analyses revealed increases in proinflammatory cytokines, especially TNF-α, IL-1α, and IL-1β. Conclusions. These data suggest that compression of a facet joint induces a novel model of local cartilage loss accompanied by increased sensitivity to mechanical stimuli and by increases in inflammatory mediators. This new model may be useful for studies on mechanisms and treatment of lumbar facet joint pain and osteoarthritis. 1. Introduction Low back pain is ubiquitous in Western society [1–3]. Its lifetime prevalence is generally accepted to be around 80% and is estimated to be one of the most costly of all medical conditions [1, 4, 5]. The majority of low back pain cases are considered to be nonspecific, with a mechanical origin [2, 6–8]. One of the structures of the spinal motion segment that has been implicated in mechanical low back pain is the lumbar facet joint; however, the contribution of facet joints to low back pain is still controversial [9–11]. Facet joints participate in load bearing in the lumbar spine during spinal motions and compressions [12–16]; they are well-innervated with nociceptors [17–26]; low back pain can be provoked in experimental conditions by irritation of the lumbar facet joints [27–29]. Anesthetic blockade can identify a contribution from facet joints in 15–67% of back pain cases [9, 11, 30–35]; neurotomy procedures can relieve chronic facet joint pain [36–39]. The human clinical studies cited above have major limitations with respect to investigating the underlying mechanisms of low back pain. While numerous animal models exist to investigate major spinal disorders [40–45], few animal models of lumbar facet joint injury exist [46–51]. To address the
Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity
Jianying Zhang, Tiffany Pan, Hee-Jeong Im, Freddie H Fu, James HC Wang
BMC Medicine , 2011, DOI: 10.1186/1741-7015-9-68
Abstract: To test the above hypothesis, we derived ligament stem cells from normal hACL and hMCL samples from the same adult donors using tissue culture techniques and characterized their properties using immunocytochemistry, RT-PCR, and flow cytometry.We found that both hACL stem cells (hACL-SCs) and hMCL stem cells (hMCL-SCs) formed colonies in culture and expressed stem cell markers nucleostemin and stage-specific embryonic antigen-4 (SSEA-4). Moreover, both hACL-SCs and hMCL-SCs expressed CD surface markers for mesenchymal stem cells, including CD44 and CD90, but not those markers for vascular cells, CD31, CD34, CD45, and CD146. However, hACL-SCs differed from hMCL-SCs in that the size and number of hACL-SC colonies in culture were much smaller and grew more slowly than hMCL-SC colonies. Moreover, fewer hACL-SCs in cell colonies expressed stem cell markers STRO-1 and octamer-binding transcription factor-4 (Oct-4) than hMCL-SCs. Finally, hACL-SCs had less multi-differentiation potential than hMCL-SCs, evidenced by differing extents of adipogenesis, chondrogenesis, and osteogenesis in the respective induction media.This study shows for the first time that hACL-SCs are intrinsically different from hMCL-SCs. We suggest that the differences in their properties contribute to the known disparity in healing capabilities between the two ligaments.The human anterior cruciate ligament (hACL) and medial collateral ligament (hMCL) are two major ligaments that function to stabilize the knee joint. Because knee joints are subjected to large mechanical loads, particularly in athletic settings, both ligaments are frequently injured. It has been established that the injured hACL rarely heals, often requiring surgical reconstruction. As a result, patients with injured ACLs typically experience recurrent instability of the knee joint [1], which could lead to development of osteoarthritis [2]. On the other hand, the injured hMCL typically heals with conservative, non-operative treatment [3,4]
Basic fibroblast growth factor induces matrix metalloproteinase-13 via ERK MAP kinase-altered phosphorylation and sumoylation of Elk-1 in human adult articular chondrocytes
Hee-Jeong Im, Andrew D Sharrocks, Xia Lin, et al
Open Access Rheumatology: Research and Reviews , 2009, DOI: http://dx.doi.org/10.2147/OARRR.S7527
Abstract: asic fibroblast growth factor induces matrix metalloproteinase-13 via ERK MAP kinase-altered phosphorylation and sumoylation of Elk-1 in human adult articular chondrocytes Original Research (3598) Total Article Views Authors: Hee-Jeong Im, Andrew D Sharrocks, Xia Lin, et al Published Date October 2009 Volume 2009:1 Pages 151 - 161 DOI: http://dx.doi.org/10.2147/OARRR.S7527 Hee-Jeong Im,1–4 Andrew D Sharrocks,5 Xia Lin,6 Dongyao Yan,1 Jaesung Kim,1 Andre J van Wijnen,7 Robert A Hipskind8 1Departments of Biochemistry, 2Internal Medicine, 3Section of Rheumatology, Orthopedic Surgery, 4Rush University Medical Center, and Department of Bioengineering; University of Illinois at Chicago, IL USA; 5Faculty of Life Sciences, University of Manchester, Oxford Rd, Manchester, UK; 6Michael D DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas, USA; 7Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA; 8Institute De Genetique Moleculaire de Montpellier, France Abstract: Degradation of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) and release of basic fibroblast growth factor (bFGF) are principal aspects of the pathology of osteoarthritis (OA). ECM disruption leads to bFGF release, which activates the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway and its downstream target the Ets-like transcription factor Elk-1. Previously we demonstrated that the bFGF-ERK-Elk-1 signaling axis is responsible for the potent induction of MMP-13 in human primary articular chondrocytes. Here we report that, in addition to phosphorylation of Elk-1, dynamic posttranslational modification of Elk-1 by small ubiquitin-related modifier (SUMO) serves as an important mechanism through which MMP-13 gene expression is regulated. We show that bFGF activates Elk-1 mainly through the ERK pathway and that increased phosphorylation of Elk-1 is accompanied by decreased conjugation of SUMO to Elk-1. Reporter gene assays reveal that phosphorylation renders Elk-1 competent for induction of MMP-13 gene transcription, while sumoylation has the opposite effect. Furthermore, we demonstrate that the SUMO-conjugase Ubc9 acts as a key mediator for Elk-1 sumoylation. Taken together, our results suggest that sumoylation antagonizes the phosphorylation-dependent transactivation capacity of Elk-1. This attenuates transcription of its downstream target gene MMP-13 to maintain the integrity of cartilage ECM homeostasis.
Basic fibroblast growth factor induces matrix metalloproteinase-13 via ERK MAP kinase-altered phosphorylation and sumoylation of Elk-1 in human adult articular chondrocytes
Hee-Jeong Im,Andrew D Sharrocks,Xia Lin,et al
Open Access Rheumatology: Research and Reviews , 2009,
Abstract: Hee-Jeong Im,1–4 Andrew D Sharrocks,5 Xia Lin,6 Dongyao Yan,1 Jaesung Kim,1 Andre J van Wijnen,7 Robert A Hipskind81Departments of Biochemistry, 2Internal Medicine, 3Section of Rheumatology, Orthopedic Surgery, 4Rush University Medical Center, and Department of Bioengineering; University of Illinois at Chicago, IL USA; 5Faculty of Life Sciences, University of Manchester, Oxford Rd, Manchester, UK; 6Michael D DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas, USA; 7Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA; 8Institute De Genetique Moleculaire de Montpellier, FranceAbstract: Degradation of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) and release of basic fibroblast growth factor (bFGF) are principal aspects of the pathology of osteoarthritis (OA). ECM disruption leads to bFGF release, which activates the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway and its downstream target the Ets-like transcription factor Elk-1. Previously we demonstrated that the bFGF-ERK-Elk-1 signaling axis is responsible for the potent induction of MMP-13 in human primary articular chondrocytes. Here we report that, in addition to phosphorylation of Elk-1, dynamic posttranslational modification of Elk-1 by small ubiquitin-related modifier (SUMO) serves as an important mechanism through which MMP-13 gene expression is regulated. We show that bFGF activates Elk-1 mainly through the ERK pathway and that increased phosphorylation of Elk-1 is accompanied by decreased conjugation of SUMO to Elk-1. Reporter gene assays reveal that phosphorylation renders Elk-1 competent for induction of MMP-13 gene transcription, while sumoylation has the opposite effect. Furthermore, we demonstrate that the SUMO-conjugase Ubc9 acts as a key mediator for Elk-1 sumoylation. Taken together, our results suggest that sumoylation antagonizes the phosphorylation-dependent transactivation capacity of Elk-1. This attenuates transcription of its downstream target gene MMP-13 to maintain the integrity of cartilage ECM homeostasis.Keywords: osteoarthritis, MMP-13, bFGF, SUMO, Elk-1
Evaluation of the Reference Numerical Parameters of the Monthly Method in ISO 13790 Considering S/V Ratio
Hee-Jeong Kwak,Jae-Hun Jo,Seung-Jik Suh
Sustainability , 2015, DOI: 10.3390/su7010767
Abstract: Many studies have investigated the accuracy of the numerical parameters in the application of the quasi steady-state calculation method. The aim of this study is to derive the reference numerical parameters of the ISO 13790 monthly method by reflecting the surface-to-volume (S/V) ratio and the characteristics of the structures. The calculation process was established, and the parameters necessary to derive the reference numerical parameters were calculated based on the input data prepared for the established calculation processes. The reference numerical parameters were then derived through regression analyses of the calculated parameters and the time constant. The parameters obtained from an apartment building and the parameters of the international standard were both applied to the Passive House Planning Package (PHPP) and EnergyPlus programs, and the results were analyzed in order to evaluate the validity of the results. The analysis revealed that the calculation results based on the parameters derived from this study yielded lower error rates than those based on the default parameters in ISO 13790. However, the differences were shown to be negligible in the case of high heat capacity.
Associations with the Interannual Variations of Onset and Withdrawal of the Changma
伴随韩国雨季开始和结束早晚的关联场分析

Lu Riyu,Huang Ronghui,Hee-Jeong Baek,Jai-Ho Oh,Baek-Jo Kim,
Lu Riyu
,Huang Ronghui,Hee-Jeong Baek,Jai-Ho Oh,Baek-Jo Kim

大气科学进展 , 2001,
Abstract: The associations of onset and withdrawal of the rainy season in South Korea (called Changma) have been examined. Composite studies showed that there are significant differences in circulations between extremely early and late onset (or withdrawals) not only over East Asia, but also over remote areas. The in situ significant differences include the upper-level jet over East Asia and the subtropical anticyclone over the western North Pacific at lower levels. The significant remote associations include the Indian monsoon and ENSO. The Indian summer monsoon is related to both onset and withdrawal of the Changma, while ENSO has a significant relation only to onset, but not to withdrawal.
Influence of the wastewater composition on denitrification and biological P-removal in the S-DN-P process: (c) Dissolved and undissolved substrates
Influence of the wastewater composition on denitrification and biological P-removal in the S-DN-P process: (c) Dissolved and undissolved substrates

Hee-Jeong Choi,Chul-Ho Choi,Seung-Mok Lee,Diwakar Tiwari,
Hee-Jeong Choi
,Chul-Ho Choi,Seung-Mok Lee,Diwakar Tiwari

环境科学学报(英文版) , 2009,
Abstract: The denitrification and P-removal in the sorption-denitrification-P-removal (S-DN-P) process were carried out under various wastewater compositions. It is noted that P-removal largely depends on the wastewater composition as well to the quantity of the substrates present in wastewater fraction. Three different wastewater fractions are obtained as: raw wastewater, dissolved wastewater (obtained with filtration using 0.45 μm filter), and undissolved wastewater (i.e., infiltrate obtained by above filtration). The ratio of P-release/CODtotal-consumption clearly inferred that undissolved wastewater possess very low value i.e., 0.0008 followed by raw wastewater 0.008 and dissolved wastewater 0.03. When this ratio was nearby 0.01, enhanced P-removal was observed. Moreover, the ratio of P-uptake to NO3 -N decomposition for raw wastewater was two times for dissolved wastewater. Interestingly, it was observed that the P-removal and denitrification depend not only on the dissolved substrates but also the undissolved substrates present in the wastewater. The result of the P-removal obtained with this S-DN-P process did not show a big difference of 36%, 34% and 30%, respectively, for raw, dissolved and undissolved wastewater.
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