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Search Results: 1 - 10 of 401402 matches for " Hebe M; Lozada "
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Bioprospection of marine microorganisms: biotechnological applications and methods
Dionisi,Hebe M; Lozada,Mariana; Olivera,Nelda L.;
Revista argentina de microbiolog?-a , 2012,
Abstract: environmental microorganisms constitute an almost inexhaustible reserve of genetic and functional diversity, accumulated during millions of years of adaptive evolution to various selective pressures. in particular, the extent of microbial biodiversity in marine habitats seems to grow larger as new techniques emerge to measure it. this has resulted in novel and more complex approaches for the screening of molecules and activities of biotechnological interest in these environments. in this review, we explore the different partially overlapping biotechnological fields that make use of microorganisms and we describe the different marine habitats that are particularly attractive for bioprospection. in addition, we review the methodological approaches currently used for microbial bioprospection, from the traditional cultivation techniques to state of the art metagenomic approaches, with emphasis in the marine environment.
Bioprospection of marine microorganisms: potential and challenges for Argentina Bioprospección de microorganismos marinos: potencialidades y desafíos para Argentina
Hebe M Dionisi,Mariana Lozada,Nelda L Olivera
Revista argentina de microbiolog?-a , 2012,
Abstract: The marine environments of Argentina have a remarkable extension, as well as high biological productivity and biodiversity of both macro- and microorganisms. Despite having a great potential for biotechnological applications, the microorganisms inhabiting these ecosystems remain mostly unexplored and unexploited. In this review, we study the research topics and the interactions among Argentinean laboratories, by analyzing current articles published on biotechnology-related marine microbiology by researchers of this country. In addition, we identify the challenges and opportunities for Argentina to take advantage of the genetic potential of its marine microorganisms. Finally, we suggest possible actions that could improve the development of this research field, as well as the utilization of this knowledge to solve societal needs. El medio ambiente marino de la Argentina tiene una notable extensión, como así también una alta productividad biológica y biodiversidad de macro y microorganismos. A pesar de presentar un gran potencial para aplicaciones biotecnológicas, los microorganismos que habitan estos ecosistemas permanecen mayormente inexplorados y sus propiedades aún no explotadas. En este trabajo de revisión, estudiamos los temas de investigación y las interacciones entre grupos de investigación argentinos, por medio del análisis de los artículos publicados hasta el momento en temáticas relacionadas con la aplicación biotecnológica de microorganismos marinos. Además, identificamos los desafíos y las oportunidades para que la Argentina tome ventaja del potencial genético de sus microorganismos marinos. Por último, sugerimos posibles acciones que podrían mejorar el desarrollo de este campo de estudio, como así también la utilización de este conocimiento para resolver las necesidades de la sociedad.
Novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of Patagonia
Mariana Lozada, Juan P Riva Mercadal, Leandro D Guerrero, Walter D Di Marzio, Marcela A Ferrero, Hebe M Dionisi
BMC Microbiology , 2008, DOI: 10.1186/1471-2180-8-50
Abstract: Genes encoding for the catalytic alpha subunit of aromatic ring-hydroxylating dioxygenases (ARHDs) were amplified from intertidal sediment samples using two different primer sets. Products were cloned and screened by restriction fragment length polymorphism analysis. Clones representing each restriction pattern were selected in each library for sequencing. A total of 500 clones were screened in 9 gene libraries, and 193 clones were sequenced. Libraries contained one to five different ARHD gene types, and this number was correlated with the number of PAHs found in the samples above the quantification limit (r = 0.834, p < 0.05). Overall, eight different ARHD gene types were detected in the sediments. In five of them, their deduced amino acid sequences formed deeply rooted branches with previously described ARHD peptide sequences, exhibiting less than 70% identity to them. They contain consensus sequences of the Rieske type [2Fe-2S] cluster binding site, suggesting that these gene fragments encode for ARHDs. On the other hand, three gene types were closely related to previously described ARHDs: archetypical nahAc-like genes, phnAc-like genes as identified in Alcaligenes faecalis AFK2, and phnA1-like genes from marine PAH-degraders from the genus Cycloclasticus.These results show the presence of hitherto unidentified ARHD genes in this sub-Antarctic marine environment exposed to anthropogenic contamination. This information can be used to study the geographical distribution and ecological significance of bacterial populations carrying these genes, and to design molecular assays to monitor the progress and effectiveness of remediation technologies.The Southwest Atlantic coast forms the border of one of the most productive marine ecosystems [1]. Extending south of 40°S latitude, the Patagonian coast holds an exceptional biodiversity, sustaining important breeding colonies and feeding grounds for seabirds and marine mammals. One of the most significant threats to the cons
Enfermería de salud pública, modernización y cooperación internacional: El proyecto de la Escuela Nacional de Enfermeras de Venezuela, 1936-1950
Vessuri, Hebe M. C.;
História, Ciências, Saúde-Manguinhos , 2001, DOI: 10.1590/S0104-59702001000400002
Abstract: in the end of 19th century, the venezuelan public health system was in the hands of religious women who ran the public health facilities. a new educational model would produce professional nurses who came to rise the standards of their occupation. undoubtedly, this objective could not be properly met due to the candidates' low educational level and the need of a health service that searched for more economical means to face its demands with trained personnel but without real educational improvements. another hindrance was the resistance of traditional medicine, which prevented a view of health as a whole, which comprehends social and preventive aspects. it was rather difficult for the founders of nursing schools to make public health facilities develop health orientation.
De la transferencia a la creatividad. Los papeles culturales de la ciencia en los países subdesarrollados
Hebe M. C. Vessuri
Polis : Revista de la Universidad Bolivariana , 2002,
Enfermería de salud pública, modernización y cooperación internacional: El proyecto de la Escuela Nacional de Enfermeras de Venezuela, 1936-1950
Vessuri Hebe M. C.
História, Ciências, Saúde-Manguinhos , 2001,
Abstract: A fines del siglo XIX, la enfermería en Venezuela estaba en manos de religiosas que atendían en centros de salud. En los a os treinta era evidente que se necesitaba un nuevo modelo para formar enfermeras profesionales que elevara los estándares de la profesión. Sin embargo, el nivel inadecuado de educación con que llegaban las aspirantes y las exigencias de los servicios hospitalarios que buscaban una manera económica de llenar sus demandas, con personal adiestrado pero sin mejoras educativas, impidió que se cumpliera esa finalidad. También dificultó el logro de los objetivos, la resistencia de la medicina curativa que impedía una visión integral de la salud, donde se incorporara la dimensión preventiva y social. Las fundadoras de la Escuela Nacional de Enfermeras en 1936-1940 encontraron dificultades para hacer de la enfermería un complemento eficaz en la orientación de salud pública.
Prepara??o e caracteriza??o de eletrodos monocristalinos
Perez, Joelma;Villullas, Hebe M.;Gonzalez, Ernesto R.;
Química Nova , 1997, DOI: 10.1590/S0100-40421997000500018
Abstract: studies of the kinetics of electrocatalytic reactions on well oriented single crystal surfaces have demonstrated the influence of surface structure on the rate and mechanisms of many electrochemical processes. the preparation and characterization of these surfaces is the first step in this type of studies. in this paper, a methodology employed in order to ensure the quality and cleanliness of single crystals and their utilization as rotating electrodes is described.
La industria de la publicación científica: Transformaciones recientes de la propiedad intelectual en el campo de las revistas científicas electrónicas
Rodríguez,Lenny; Vessuri,Hebe M.C;
Revista Venezolana de Economía y Ciencias Sociales , 2007,
Abstract: recently there has been a growing awareness of the role of intellectual property rights as a growth factor. this article aims to examine the changes in the notion of intellectual property rights as scientific and other forms of communication have evolved. the main objective is to offer a panoramic view of the current tensions and contradictions between the actors involved in the problem of intellectual property and in the handling of related information. on the one hand, there is the scientific community, that has seen its rights affected; on the other, the editors or publishers, who see their profit margins threatened, as they begin to lose their control and exclusivity in the publication and dissemination of information. finally, the readers are beginning to fulfill a different role, where an active participation begins to influence the process.
Ciencia, tecnología y desarrollo: una experiencia de apropiación social del conocimiento
Interciencia , 2002,
Abstract: this paper is about a social process. as a part of the ongoing revolution in computer mediated technologies, an analysis is made of the potential offered, from the perspective of the social appropriation of knowledge, by a new website for global dialogue in the field of science, technology, development and innovation that aims to an audience including all those with either a professional or personal interest in the role of science and technology in sustainable development. a brief opening discussion is made of the difficulties of improving the social understanding of science in developing countries. then scidev.net is analyzed as a project trying to build up a social web around scientific issues from the perspective of the south. the strategic aims of the website, as well as issues like the existence of hidden agendas in many of those sites, the relevance of the topics covered, the directionality of the flows of information, audiences targeted by the website, the challenges posed for building up a more harmonic international collaboration in this field, are all paid attention.
Saber leer, aprender a leer
Tizio, Hebe;
Educa??o em Revista , 2009, DOI: 10.1590/S0102-46982009000100012
Abstract: following the observation that, nowadays, people read less, the author places another question: do people read less or do they just read differently? thus, the article interrogates the relation of the talking being to writing and culture, highlighting in the reading process the interpretation component which does not occur apart from the unconscious reading. it presents the unconscious as an interpreter, to wit, a reading device that interprets reality and everything that is read. it draws attention to the dimension of satisfaction inherent in the interpretation activity and in the schooling function to spot possibilities so that the said satisfaction may spread socially.
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