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Search Results: 1 - 10 of 402629 matches for " Heather M Mottaz "
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Integrative Analysis of the Mitochondrial Proteome in Yeast
Holger Prokisch,Curt Scharfe,David G. Camp II,Wenzhong Xiao,Lior David,Christophe Andreoli,Matthew E. Monroe,Ronald J. Moore,Marina A. Gritsenko,Christian Kozany,Kim K. Hixson,Heather M. Mottaz,Hans Zischka,Marius Ueffing,Zelek S. Herman,Ronald W. Davis,Thomas Meitinger,Peter J. Oefner,Richard D. Smith,Lars M. Steinmetz
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0020160
Abstract: In this study yeast mitochondria were used as a model system to apply, evaluate, and integrate different genomic approaches to define the proteins of an organelle. Liquid chromatography mass spectrometry applied to purified mitochondria identified 546 proteins. By expression analysis and comparison to other proteome studies, we demonstrate that the proteomic approach identifies primarily highly abundant proteins. By expanding our evaluation to other types of genomic approaches, including systematic deletion phenotype screening, expression profiling, subcellular localization studies, protein interaction analyses, and computational predictions, we show that an integration of approaches moves beyond the limitations of any single approach. We report the success of each approach by benchmarking it against a reference set of known mitochondrial proteins, and predict approximately 700 proteins associated with the mitochondrial organelle from the integration of 22 datasets. We show that a combination of complementary approaches like deletion phenotype screening and mass spectrometry can identify over 75% of the known mitochondrial proteome. These findings have implications for choosing optimal genome-wide approaches for the study of other cellular systems, including organelles and pathways in various species. Furthermore, our systematic identification of genes involved in mitochondrial function and biogenesis in yeast expands the candidate genes available for mapping Mendelian and complex mitochondrial disorders in humans.
Integrative Analysis of the Mitochondrial Proteome in Yeast
Holger Prokisch equal contributor,Curt Scharfe equal contributor,David G Camp II equal contributor,Wenzhong Xiao equal contributor,Lior David,Christophe Andreoli,Matthew E Monroe,Ronald J Moore,Marina A Gritsenko,Christian Kozany,Kim K Hixson,Heather M Mottaz,Hans Zischka,Marius Ueffing,Zelek S Herman,Ronald W Davis,Thomas Meitinger,Peter J Oefner,Richard D Smith,Lars M Steinmetz
PLOS Biology , 2004, DOI: 10.1371/journal.pbio.0020160
Abstract: In this study yeast mitochondria were used as a model system to apply, evaluate, and integrate different genomic approaches to define the proteins of an organelle. Liquid chromatography mass spectrometry applied to purified mitochondria identified 546 proteins. By expression analysis and comparison to other proteome studies, we demonstrate that the proteomic approach identifies primarily highly abundant proteins. By expanding our evaluation to other types of genomic approaches, including systematic deletion phenotype screening, expression profiling, subcellular localization studies, protein interaction analyses, and computational predictions, we show that an integration of approaches moves beyond the limitations of any single approach. We report the success of each approach by benchmarking it against a reference set of known mitochondrial proteins, and predict approximately 700 proteins associated with the mitochondrial organelle from the integration of 22 datasets. We show that a combination of complementary approaches like deletion phenotype screening and mass spectrometry can identify over 75% of the known mitochondrial proteome. These findings have implications for choosing optimal genome-wide approaches for the study of other cellular systems, including organelles and pathways in various species. Furthermore, our systematic identification of genes involved in mitochondrial function and biogenesis in yeast expands the candidate genes available for mapping Mendelian and complex mitochondrial disorders in humans.
Global Systems-Level Analysis of Hfq and SmpB Deletion Mutants in Salmonella: Implications for Virulence and Global Protein Translation
Charles Ansong, Hyunjin Yoon, Steffen Porwollik, Heather Mottaz-Brewer, Brianne O. Petritis, Navdeep Jaitly, Joshua N. Adkins, Michael McClelland, Fred Heffron, Richard D. Smith
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0004809
Abstract: Using sample-matched transcriptomics and proteomics measurements it is now possible to begin to understand the impact of post-transcriptional regulatory programs in Enterobacteria. In bacteria post-transcriptional regulation is mediated by relatively few identified RNA-binding protein factors including CsrA, Hfq and SmpB. A mutation in any one of these three genes, csrA, hfq, and smpB, in Salmonella is attenuated for mouse virulence and unable to survive in macrophages. CsrA has a clearly defined specificity based on binding to a specific mRNA sequence to inhibit translation. However, the proteins regulated by Hfq and SmpB are not as clearly defined. Previous work identified proteins regulated by hfq using purification of the RNA-protein complex with direct sequencing of the bound RNAs and found binding to a surprisingly large number of transcripts. In this report we have used global proteomics to directly identify proteins regulated by Hfq or SmpB by comparing protein abundance in the parent and isogenic hfq or smpB mutant. From these same samples we also prepared RNA for microarray analysis to determine if alteration of protein expression was mediated post-transcriptionally. Samples were analyzed from bacteria grown under four different conditions; two laboratory conditions and two that are thought to mimic the intracellular environment. We show that mutants of hfq and smpB directly or indirectly modulate at least 20% and 4% of all possible Salmonella proteins, respectively, with limited correlation between transcription and protein expression. These proteins represent a broad spectrum of Salmonella proteins required for many biological processes including host cell invasion, motility, central metabolism, LPS biosynthesis, two-component regulatory systems, and fatty acid metabolism. Our results represent one of the first global analyses of post-transcriptional regulons in any organism and suggest that regulation at the translational level is widespread and plays an important role in virulence regulation and environmental adaptation for Salmonella.
Multi-Band Rectangular Patch End-Fire Antenna Array  [PDF]
Liza M. Resley, Heather H. Song
Journal of Electromagnetic Analysis and Applications (JEMAA) , 2013, DOI: 10.4236/jemaa.2013.52010
Abstract:

A novel multi-band end-fire antenna array was designed, fabricated, and characterized. Analytical calculations were carried out to determine the critical antenna dimensions and the design was optimized using a 3D electromagnetic finite-element solver. The measured results were in good agreement with the designed results. The proposed antenna array exhibits multi-band capabilities which can be potentially used for applications that require a multi-band end-fire radiation pattern.

Microbiologists Just Want to Have Fun
Heather M. Seitz
Journal of Microbiology & Biology Education , 2012, DOI: 10.1128/jmbe.v13i2.490
Abstract: Review of: Non-technical online microbial resources that entertain, engage, and promote microbiology thorugh music, comics, and gaming. Resources include Food Safety Music, Pandemic 2, Microbe Kombat, and The Great Flu.
Measurement of the b-jet cross-section with associated vector boson production with the ATLAS experiment at the LHC
Gray Heather M.
EPJ Web of Conferences , 2012, DOI: 10.1051/epjconf/20122812050
Abstract: A measurement of the cross-section for vector boson production in association with jets containing b-hadrons is presented using 35 pb-1 of data from the LHC collected by the ATLAS experiment in 2010. Such processes are not only important tests of pQCD but also large, irreducible backgrounds to searches such as a low mass Higgs boson decaying to pairs of b-quarks when the Higgs is produced in association with a vector boson. Theoretical predictions of the V+b production rate have large uncertainties and previous measurements have reported discrepancies. Cross-sections measured using in the electron and muon channels will be shown. Comparisons will be made to recent theoretical predictions at the next-to-leading order in αS.
Testing for Heterogeneous Factor Loadings Using Mixtures of Confirmatory Factor Analysis Models
Heather M. Buzick
Frontiers in Psychology , 2010, DOI: 10.3389/fpsyg.2010.00165
Abstract: The current study assessed the viability of mixture confirmatory factor analysis (CFA) for measurement invariance testing by evaluating the ability of mixture CFA models to identify differences in factor loadings across populations with identical mean structures. Using simulated data from a model with known parameters, convergence rates, parameter recovery, and the power of the likelihood-ratio test were investigated as impacted by sample size, latent class proportions, magnitude of factor loading differences, percentage of non-invariant factor loadings, and pattern of non-invariant factor loadings. Results suggest that mixture CFA models may be a viable option for testing the invariance of factor loadings; however, without differences in latent means and measurement intercepts, results suggest that larger sample sizes, more non-invariant factor loadings, and larger amounts of heterogeneity are needed to successfully estimate parameters and detect differences across latent classes.
The Bar-Natan skein module of the solid torus and the homology of (n,n) Springer varieties
Heather M. Russell
Mathematics , 2008,
Abstract: This paper establishes an isomorphism between the Bar-Natan skein module of the solid torus with a particular boundary curve system and the homology of the (n,n) Springer variety. The results build on Khovanov's work with crossingless matchings and the cohomology of the (n,n) Springer variety. We also give a formula for comultiplication in the Bar-Natan skein module for this specific three-manifold and boundary curve system.
A topological construction for all two-row Springer varieties
Heather M. Russell
Mathematics , 2010,
Abstract: Springer varieties appear in both geometric representation theory and knot theory. Motivated by knot theory and categorification Khovanov provides a topological construction of $(n/2, n/2)$ Springer varieties. We extend Khovanov's construction to all two-row Springer varieties. Using the combinatorial and diagrammatic properties of this construction we provide a particularly useful homology basis and construct the Springer representation using this basis. We also provide a skein-theoretic formulation of the representation in this case.
An explicit bijection between semistandard tableaux and non-elliptic sl_3 webs
Heather M. Russell
Mathematics , 2012,
Abstract: The sl_3 spider is a diagrammatic category used to study the representation theory of the quantum group U_q(sl_3). The morphisms in this category are generated by a basis of non-elliptic webs. Khovanov- Kuperberg observed that non-elliptic webs are indexed by semistandard Young tableaux. They establish this bijection via a recursive growth algorithm. Recently, Tymoczko gave a simple version of this bijection in the case that the tableaux are standard and used it to study rotation and joins of webs. We build on Tymoczko's bijection to give a simple and explicit algorithm for constructing all non-elliptic sl_3 webs.
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