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Harsh Mittal,Manoj Jain,Latha Banda
International Journal of Computer Science and Mobile Computing , 2013,
Abstract: The project aim is to secure the network or a LAN by implementing such a software which isenable to carry out operations which are capable to monitor whole of the network ,sitting on one chair byviewing remote desktop ,passing messages to remote system and is also able to shut down the system byperforming remote aborting operations . This software is purely developed in JAVA RMI (REMOTEMETHOD INVOCATION). This project is to provide the maximum details about the network to theadministrator on their screen without knowing them their users. The administrator can view the static imageof client’s desktop and then he/she could sends warning message to the user to stop that operationimmediately. Even than if client do not stops than administrator has the facility to abort the system remotelyor restart the system whatever necessary he thinks.
Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
Harsh Vardhan,Apurb R. Bhengraj,Rajneesh Jha,Aruna Singh Mittal
Journal of Biomedicine and Biotechnology , 2009, DOI: 10.1155/2009/342032
Abstract: Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron.
Modulation of cytokines and transcription factors (T-Bet and GATA3) in CD4 enriched cervical cells of Chlamydia trachomatis infected fertile and infertile women upon stimulation with chlamydial inclusion membrane proteins B and C
Rishein Gupta, Harsh Vardhan, Pragya Srivastava, Sudha Salhan, Aruna Mittal
Reproductive Biology and Endocrinology , 2009, DOI: 10.1186/1477-7827-7-84
Abstract: A total of 14 CT-positive fertile, 18 CT-positive infertile women and 25 uninfected controls were enrolled in this study. CD8 depleted, CD4 enriched cervical cells were isolated and upon stimulation with IncB and IncC, modulation of cytokines (Interleukin (IL)-1 Beta, IL-4, IL-5, IL-6, IL-10, Interferon-gamma, IL-12, IL-23, Tumor Necrosis Factor-alpha and Granulocyte macrophage colony-stimulating factor (GM-CSF) and T cell lineage regulating transcription factors T-Bet and GATA3 was determined by real-time reverse-transcriptase (RT)-PCR and ELISA.Significant higher expression (P < 0.05) of Interferon-gamma, IL-12, IL-23 and GM-CSF were found in Inc-stimulated CD4 enriched cervical cells of CT-positive fertile women and contrastingly high IL-1 Beta, IL-4, IL-5, IL-6 and IL-10 levels were found in CT-positive infertile women. Positive correlation (P < 0.05) was found between Interferon-gamma and T-Bet levels in CT-positive fertile women and IL-4 mRNA and GATA3 levels in CT-positive infertile patients upon IncB and IncC stimulation.Overall our data shows that CT IncB and IncC are able to upregulate expression of cytokines, namely interferon-gamma, IL-12, IL-23 and GM-CSF in CT-positive fertile women while expression of IL-1 Beta, IL-4, IL-5, IL-6 and IL-10 were upregulated in CT-positive infertile women. Our study also suggests that Incs are able to modulate expression of T cell lineage determinants indicating their involvement in regulation of immune cells.Chlamydia trachomatis (CT) is an obligate intracellular pathogen and is the leading cause of sexually transmitted diseases (STD) globally [1] Chlamydial infection of the lower genital tract ascends to the upper genital tract and results in serious consequences to reproductive health, such as infertility, ectopic pregnancy, and pelvic inflammatory disease (PID) [2]. In India, a high chlamydial prevalence rate has been reported among symptomatic women with or without fertility-related disorders [3-6].CT infection and
Host immune responses to chlamydial inclusion membrane proteins B and C in Chlamydia trachomatis infected women with or without fertility disorders
Rishein Gupta, Pragya Srivastava, Harsh Vardhan, Sudha Salhan, Aruna Mittal
Reproductive Biology and Endocrinology , 2009, DOI: 10.1186/1477-7827-7-38
Abstract: Female patients (n = 296) attending the gynaecology out patient department of Safdarjung hospital, New Delhi were enrolled for the study and were clinically characterized into two groups; CT-positive fertile women (n = 38) and CT-positive women with fertility disorders (n = 29). Uninfected healthy fertile women were enrolled as controls (n = 31). Gene specific PCRs were used for detection of incB and incC genes in endocervical samples of CT-positive patients. ELISA and Western blot assay were used for detection of IgA and IgG antibodies to IncB and IncC in cervical washes and sera. Effect of IncB and IncC stimulation of cervical cells and PBMCs on cellular proliferation and cytotoxity was determined using MTT assay and Lactate dehydrogenase (LDH)-cytotoxicity assay respectively. Modulation of cytokines (Interleukin (IL)-1 Beta, IL-4, IL-5, IL-6, IL-10, Interferon-gamma, IL-12, Tumor Necrosis Factor-alpha and Granulocyte macrophage colony-stimulating factor (GM-CSF)) in cervical cells and PBMCs upon stimulation with IncB and IncC was determined by real-time reverse-transcriptase (RT)-PCR and ELISA. Further, CD4 positive T cells were purified from cervical cells and peripheral blood mononuclear cells (PBMCs) and secreted cytokines (Interferon-gamma and IL-4) were evaluated by ELISPOT and real-time RT-PCR.Using MTT assay, significantly high proliferative responses (P < 0.05) were observed in inc-stimulated cervical cells and PBMCs from CT-positive fertile women compared to CT-positive women with fertility disorders and controls. Interferon-gamma, IL-12 and GM-CSF were found to be elevated in inc-stimulated cervical cells and PBMCs of CT-positive fertile women compared to CT-positive women with fertility disorders and controls (P < 0.05). In contrast, IL-1 Beta, IL-4, IL-5, IL-6 and IL-10 levels were found to be higher in CT-positive women with fertility disorders compared to CT-positive fertile women and controls (P < 0.05). Interferon-gamma secreting cells and mRNA ex
The conundrum of lenticular oncology. A review
Chaturvedi Sunil,Mehrotra Amar,Mittal Sanjeev,Bahadur Harsh
Indian Journal of Ophthalmology , 2003,
Abstract: It is a long - accepted dogma in ophthalmology that the lens is a tumour-free tissue. Yet, in the lens, there is lifelong mitotic activity in the subcapsular epithelium. Therefore, these subcapsular epithelial cells must have the potential for cellular transformation. How then can we explain the fact that no scientist has ever seen a naturally occurring primary tumour of the lens in vivo ? This review discusses the early work of Mann, von Hallermann, Courtois and others who addressed the issue of tumour resistance of the lens.
Smartphone Based Cervical Spine Stress Prevention  [PDF]
Harsh Gupta
Journal of Software Engineering and Applications (JSEA) , 2018, DOI: 10.4236/jsea.2018.112006
Abstract: Rapidly increasing smartphone usage has resulted in an increasingly adverse impact on the health of people. Cervical Spine Stress and the risk of Thoracic Kyphosis have been accentuated due to inappropriate posture. Existing efforts to solve this problem require the use of expensive wearable devices or can only be implemented on selected smartphones. This paper has proposed a novel posture detecting mechanism which does not require any external devices and can be implemented inexpensively on all smartphones. The solution has utilized the built-in 3-dimensional accelerometer of the smartphone and provided an Android Operating System-based application as a proof of concept that determines the Cervical Spine posture and generates ALERTS when necessary. In addition, data of the posture could be displayed in form of graphs to make the user aware of his or her posture trend throughout the week. The application can be extended to different operating systems.
Higher incidence of persistent chronic infection of Chlamydia pneumoniae among coronary artery disease patients in India is a cause of concern
Hem C Jha, Harsh Vardhan, Rishein Gupta, Rakesh Varma, Jagdish Prasad, Aruna Mittal
BMC Infectious Diseases , 2007, DOI: 10.1186/1471-2334-7-48
Abstract: Venous blood was obtained from 91 CAD patients and 46 healthy controls. Nucleic acid amplification tests viz. nested -, semi-nested – and multiplex PCR were used for detection of C. pneumoniae. ELISA carried out prevalence of C. pneumoniae specific IgG and IgA antibodies.29.67% (27/91) patients were positive for C. pneumoniae using nested PCR. The sensitivity and specificity of semi-nested and multiplex PCR were 37.03%, 96.96% and 22.22%, 100% with respect to nested PCR. Positive nPCR patients were compared with presence of C. pneumoniae specific IgA, IgA+IgG and IgG antibodies. Among 27 (29.67%) nPCR C. pneumoniae positive CAD patients, 11(12%) were IgA positive, 13(14.2%) were IgA+IgG positive and only1 (1.1%) was IgG positive. A significant presence of C. pneumoniae was detected in heavy smokers, non-alcoholics and with family histories of diabetes and blood pressure group of CAD patients by nPCR.The results indicate synergistic association of C. pneumoniae infection and development of CAD with other risk factors. We also detected increased positivity for C. pneumoniae IgA than IgG in nPCR positive CAD patients. Positive nPCR findings in conjunction with persisting high C. pneumoniae specific antibody strongly suggest an ongoing infection.Coronary artery disease (CAD) is a major cause of morbidity and mortality in humans and is predicted to be the leading cause of death in the world [1]. Acquired metabolic abnormalities like hypercholesterolemia, diabetes mellitus are major risk factors associated with CAD, besides inheritance. These factors on compounding with pathogens like C. pneumoniae, Helicobacter pylori and Cytomegalovirus intensifies the magnitude of risk impending towards CAD [2,3]. Several reports have suggested a role of chronic C. pneumoniae infection in pathogenesis of CAD and other atherosclerotic syndromes [4,5]. C. pneumoniae has been established as an important pathogen that causes infections of upper and lower respiratory tract [6-8]. C. pneumon
Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron
Harsh Vardhan,Raini Dutta,Vikas Vats,Rishein Gupta,Rajneesh Jha,Hem Chandra Jha,Pragya Srivastava,Apurb Rashmi Bhengraj,Aruna Singh Mittal
Mediators of Inflammation , 2009, DOI: 10.1155/2009/417658
Abstract: Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.
Butterfly Diversity of Indian Institute of Forest Management, Bhopal, Madhya Pradesh, India
Sprih Harsh
Journal of Insects , 2014, DOI: 10.1155/2014/254972
Abstract: A study to find out the diversity of butterflies at the Indian Institute of Forest Management (IIFM), Bhopal, was carried out over a period of six months from October 2013 to March 2014. A total of 55 butterfly species belonging to 5 families, namely, Hesperiidae (7 species), Papilionidae (4 species), Pieridae (10 species), Lycaenidae (13 species), and Nymphalidae (21 species), were recorded (with photographic record) during the study from three different habitats of campus: open scrub, dry deciduous, and urbanized habitat. Shannon diversity indices and Pielou’s evenness index were calculated for all the habitats. Shannon index was found to be highest for open scrub (3.76). Out of 54 species, Eurema brigitta was the most dominant species followed by Eurema hecabe, Junonia lemonias, and Phalanta phalantha. Dominance of these species can be explained by the presence of their larval and host plants in the campus. 1. Introduction Butterflies are one of the most conspicuous species of Earth’s biodiversity. Being extremely responsive to any changes in their environment, namely, temperature, humidity, light, and rainfall patterns [1–4], these insects are identified as useful bioindicators. They have different requirements for different habitat types for mating, breeding, and nectaring and are, thus, in sync with the diversity and quality of their habitats. The present study aims to examine the diversity and distribution of butterflies across three different habitats, namely, dry deciduous, open scrub, and urbanized habitat. A checklist of butterfly species is also provided. 2. Materials and Methods 2.1. Study Area The study was done in Indian Institute of Forest Management, Bhopal (23.208371°N and 77.384417°E), from July 2012 to March 2013. The location of campus, built on a hill at an elevation of about 556?m and surrounded by water on three sides, along with the wide range of climatic conditions that it passes through brings in diverse structure of habitats. The major types of vegetation included grasslands, open scrub forest, and dry deciduous forest and bamboo groves. The study was conducted in 12 transects (each approximately 300 to 500?m long) covering an area of 93-hectare campus. For our study, transects were divided into three habitats according to general landscape attributes and vegetation present there. The chief habitat types were as follows: (1) open scrub, (2) dry deciduous, and (3) urbanized habitat. 2.2. Butterfly Survey The survey of butterfly was done using Pollard walk method [5, 6] from 8 am to 10 am. The butterflies were observed within
The L1 norm of the generalized de la Vallee Poussin kernel
Harsh Mehta
Mathematics , 2013,
Abstract: Charles de la Vall'ee Poussin defined two different kernels that bear his name. This paper considers the one are a linear combinations of two Fej'er kernels, which are known as the delayed means. We show that the $L^1$ norms are constant in families of delayed means, and determine the exact value
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