OALib Journal期刊

ISSN: 2333-9721



匹配条件: “Hajrulai-Musliu-Zehra” ,找到相关结果约220条。
Analysis of 226Ra, 232Th 40К and 137Cs in samples of soil from some areas of Republic of Macedonia by using gamma spectrometry
Todorovik Aleksandra,Dimitrieska Stojkovik Elizabeta,Hajrulai-Musliu-Zehra,Uzunov Risto
Macedonian Veterinary Review , 2012,
Abstract: Taking into consideration the importance of the distribution and transfer of radio nuclides in soil, an attempt was made in this work to determine the concentration of 226Ra, 232Th 40К and 137Cs in the same. The concentrations of activity in the gamma-absorbed dose rates of the terrestrial naturally occurring radio nuclides, as follows, 226Ra, 232Th and 40K were determined in samples of soil collected from some parts of Republic of Macedonia, i.e. from three major cities in the Republic of Macedonia. The samples are taken by means of a special dosage dispenser which enables sampling of samples at a depth of 0-5 cm, 5-10cm and 10-15cm, thus disabling the sampling above these layers of soil. An identification of radio nuclides and assessment of their activity has been performed by applying gamma spectrometry. The time of counting for each sample was 65000 s. in order to obtain statistically small mistake. The spectrums were analyzed by a commercially available software GENIE-2000 received from Canberra, Austria. The activity of soil had wide range of values: 20.3 to 82.9 Bq kg-1for 226Ra, 16.1 to 82.5 Bq kg-1 for 232Th, 325 to 799.0 Bq kg-1for 40К and 9.1 to 24.3 Bq kg-1for 137Cs, respectively. The concentrations of these radio nuclides have been compared with the available data from the other countries. Natural environmental radioactivity and the associated external exposure due to gamma radiation depend primarily on the geological and geographical conditions. Namely, the specific levels of terrestrial environmental radiation are related to the type of rocks from which the soils originate. The obtained data indicate that the average value of activity of 232Th is about higher than the one of 226Ra The concentration of activity of 40К in the soil has greater value than 32Th and 226Ra in all soils. The causes for the existence of 137Cs in these soils are the nuclear explosions, waste radioactive materials and other incidents. It reaches the surface of the pedosphere by means of dry and wet flushing and depending on the physical and the chemical properties of the soil, as well as depending on the eventually applied agrotechnical and agrochemical measures, it connects, that is, it penetrates in the deeper soil layers.
In-house validation and quality control of commercial enzyme-linked immunosorbnet assays for screening of nitrofuran metabolites in food of animal origin
Dimitrieska-Stojkovic Elizabeta,Arsova Gordana,Hajrulai-Musliu Zehra,Stojanovska-Dimzoska Biljana
Macedonian Veterinary Review , 2012,
Abstract: Application of nitrofuran antimicrobials at food production animals was prohibited by Commission Regulation 2003/181/EC because of their potential carcinogenic and mutagenic effects on humans. Main protein-bound metabolites of nitofurans are 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ), 1-aminohydantoin (AHD), semicarbazide (SEM) and 3-amino-2-oxazolidinone (AOZ). Since then numerous costly liquid chromatography with tandem mass spectrometry (LC/MS/MS) methods have been developed for screening and confirmation of nitrofuran metabolites in line with the EU requirements for performing official controls. As an inexpensive and less time consuming alternative, enzyme-immunoassay methods were developed for screening of the respective compounds. In this study validation and evaluation of four commercial enzyme-linked immunosorbent assay (ELISA) has been performed. According to the requirements of Commission Decision 2002/657/EC, different performance characteristics (specificity, detection capability, precision) for various matrices (liver, eggs, honey) have been determined for each kit. The validation study has confirmed that the methods studied possess suitable characteristics: detectionlimits between 0.126 and 0.240 μg/kg, detection capabilities ≤1.0 μg/kg and the inter-day precision in the range from 16.20% to 22.11 %. The validation study was finalized by participation in FAPAS Proficiency testing scheme in 2011, and the obtained results have confirmed the capability of applied methods for unambiguous discrimination between negative and positive sample.
Validation of screening method for determination of methyltestosterone in fish
Uzunov Risto,Hajrulai-Musliu Zehra,Stojanovska-Dimzoska Biljana,Dimitrieska-Stojkovic Elizabeta
Macedonian Veterinary Review , 2013,
Abstract: Anabolic androgenic steroids are synthetic derivatives of testosterone, which is the primary male sex hormone. These anabolic agents are used to increase the weight gain, to improve the food efficiency, storing proteins and to decrease fatness. However, depending on the use of anabolic agent in animal feed, anabolic residues that may occur in meat and meat products present risks to human health. The aim of this study was the validation of screening ELISA method for determination of methyltestoterone anabolic steroid in fish. The validation process was carried out according to Commission Decision 2002/657/EC criteria. The detection limit for methyltestosterone was 140.95 ng/kg and the detection capability was 564.43 ng/kg. The overall recoveries and the coefficients of variation (CV) were in the range of 82.4%-97.4% and 1.5%-6.9%, respectively, a working range between 50 to 4050 ng/kg, and the regression equation of the final inhibition curve was: y= -0,1741x + 1,5082, R2 = 0.9927. Because of the good recovery and precision, and satisfactory detection capability, this method is applicable in official control laboratories as a rapid screening method for determination of methyltestosterone in fish.
Screening of veterinary drug residues in milk from individual farms in Macedonia
Dimitrieska-Stojkovic Elizabeta,Hajrulai-Musliu Zehra,Stojanovska-Dimzoska Biljana,Sekulovski Pavle
Macedonian Veterinary Review , 2011,
Abstract: A total of 497 raw milk samples collected at individual farms and collection tanks for milk from eight regions from Macedonia were examined for chloramphenicol, sulfonamides, quinolones and tetracyclines from October 2008 until April 2011. Immunoassay methods were used for the determination of chloramphenicol, sulfonamides and quinolones, and high performance liquid chromatography with Diode Array detection was applied for screening of tetracyclines. The methods were validated according to the recommendations laid down by European Commission Decision 2002/657/EC. The obtained data confirmed that the methods were appropriate for detection of antibiotics determined, at the concentration level of interest. Measured range of concentrations (in μg/kg) was 13.5-147.9 for sulfonamides, 0.6-22.0 for quinolones and 17.4-149.1 for tetracyclines, with calculated mean values (in μg/kg) 24.7 for sulfonamides, 12.6 for qinolones and 41.9 for tetracyclines. None of the analyzed samples showed presence of chloramphenicol over the minimum required performance level value of the screening method. The calculated estimated daily intakes for the average daily consumption of 200 mL of milk for an adult in Macedonia, for the examined antimicrobials, obtained levels 2 to 100 times lower than the values of the acceptable daily intakes fixed by World Health Organization. This indicates that toxicological risk associated with the consumption of analyzed milk could not be considered as a public health issue with regards to these veterinary drugs.
Occurrence of ochratoxin a in macedonian wines from tikves region
Stojanovska-Dimzoska Biljana,Dimitrieska-Stojkovic Elizabeta,Hajrulai-Musliu Zehra,Sekulovski Pavle
Macedonian Veterinary Review , 2008,
Abstract: An analytical method based on immunoaffinity column (IAC) clean-up and quantitative determination with liquid chromatography - fluorescence detection (LC-FD) was used to determine the occurrence of ochratoxin A (OTA) in Macedonian wines originated from the most famous, Tikves, wine producing region. The linearity of the method was checked, and a good coefficient of correlation (0.9976) was found, over wide concentration range of 0,1 to 60 ng/ml. The limit of detection and quantification were 0,043 ng/ml and 0,131 ng/ml, respectively. The accuracy of the method was determined with spiked OTA free samples at the concentration levels of 0.1 ng/ml and 1.0 ng/ml. The recoveries for red wine were found to be 100,66% and 106,83 %, for the proposed spiking levels. The recoveries for white wine were satisfactory (98,50% and 93,31%) too, in the same spiking levels. RSD values for both, red and white wine samples, for the proposed concentration level was satisfactory, in the rang of 7,13% to 10,41% for red wine and 3,96% to 8,77% for white wine samples. A survey was done on 31 bottled wine samples. Among them, 16 samples (51,61%) were with OTA concentration over the LOD. However, the determined concentrations were too low to present a risk for wine consumers.
Determination of total aflatoxines in dryed and nuted fruits present in macedinian market
Hajrulai-Musliu Zehra,Sekulovski Pavle,Stojanovska Dimzoska Biljana,Dimitrieska Stojkovic Elizabeta
Macedonian Veterinary Review , 2008,
Abstract: Humans and animals are exposed to aflatoxins by consuming foods contaminated with products of fungal growth. Such exposure is difficult to avoid because fungal growth in foods is not easy to prevent. Even though heavily contaminated food supplies are not distributed at market in developed countries, concern still remains for the possible adverse effects as a consequence of long-term exposure to low levels of aflatoxins in the food supply. Thence the aim of this study was determination of total aflatoxins in dry fruits and nuts. Only products in the open market places such as peanuts, walnuts, hazelnuts, pumpkin seeds and raisins, were analysed. Nineteen of 30 analysed samples (63.33%) were over the detection limit, whereas 11 of analysed samples (36,6%) were the same limit.The highr then allowed value of aflatoxins concentration was determined only in one sample of walnut (21 μg/kg).
Comparison of two different analytical methods for determination of aflatoxins in feed
Stojanovska-Dimzoska Biljana,Hajrulai-Musliu Zehra,Dimitrieska-Stojkovic Elizabeta,Enimiteva Vangelica
Macedonian Veterinary Review , 2009,
Abstract: Liquid chromatography - fluorescence detector and fluorometry (fluorescence spectrometry) with previous immunoaffinity column clean-up, are two methods that are compared in order to evaluate their performances in determination of total aflatoxins content in feed. Method validation was established, linearity of the methods was checked, and a good coefficient of correlation was found for bought methods (0,9994 and 0,978 respectively). The limits of detection were 0,003 ng/ml (average for HPLC-FD method) and 0,12 ng/ml for fluorometry. The accuracy of the methods was determined using reference materials with known concentration and spiked aflatoxins free samples. The recoveries were found to be 85,82% (average for HPLC-FD method) and 56,75%, for fluorometry. Twenty feed concentrates for bullock and 20 feed concentrates for swine, were analyzed parallel with bought methods. The results are in correlation only in higher concentration range. For lower concentration range (below 1,0 μg/kg) fluorometry shows limited sensitivity and selectivity. It was found that it is applicable only as a screening method for the prediction of aflatoxins. HPLC-FD method remains a method of choice for the analyst in order to achieved accurate and reliable results.
Determination of aflatoxins in foodstuffs employing fluorometric method
Dimitrieska-Stojkovi? Elizabeta D.,Danev Mihail M.,Hajrulai-Musliu Zehra B.,Stojanovska-Dimzoska Biljana S.
Zbornik Matice Srpske za Prirodne Nauke , 2005, DOI: 10.2298/zmspn0508031d
Abstract: Results from the examination of total aflatoxins in foodstuffs are presented Determination of the aflatoxins was done employing rapid and reliable method based on immunnosorbent columns clean-up and measurement of the aflatoxins by fluorometer (Vicam, USA). Quantification and detection limits of the method were 1.0 and 0.1 ppb respectively. This method is especially suitable when laboratories are dealing with big number of samples. 20% of the samples were found to content total aflatoxins over MRL (5 g/kg). The positive samples were further analysed with TLC as a confirmatory method, whereby over 95% of positive samples where confirmed by TLC technique. Application of this rapid aflatoxin method significantly simplifies their analysis, especially when laboratories are dealing with big number of samples.
Listeria monocytogenes contamination of the environment and surfaces of the equipment in the meat processing facilities in republic of Macedonia
Dean Jankuloski,Pavle Sekulovski,Risto Prodanov,Zehra Hajrulai Musliu
Macedonian Veterinary Review , 2007,
Abstract: Listeria monocytogenes contamination of the environment and surfaces of the equipment was examined in seven meat processing facilities. Up to date prevalence of this foodborn pathogen in meat processing facilities facilities in Republic of Macedonia was unknown. Biofilms are composed from food spoilage microorganisms and food born pathogens. They are located on the surfaces of the equipment that come in contact with food and in facilities environment. Microorganisms in biofilm presenting micro eco system and are source of dissemination and contamination of food born pathogens in final meat products. During the preparation of this study we have covered a 7 meat processing facilities and we took a total of 39 swabs from surfaces that come in direct or indirect contact with food. Listeria monocytogenes was discovered in 10 (25,64%) swabs (locations). Prevalence of other Listeria spp. compared with total number of taken samples was 15 (38,46%) Listeria innocua, 3 (7,69%) Listeria welshimeri and 1 (2,65%) isolate Listeria seeligeri.
Delayed Puberty
Turk Pediatri Ar?ivi , 2011,
Abstract: Puberty is a quite important phase in the physical and psychosocical development of the adolescent. The timing of puberty is variable and is dependent on several factors including ethnic group, nutrition and genetic factors. It may commence from 8 years of age in girls and 9 years in boys and is usually complete after 3,5 years in female and 2,5 years in males. Delayed puberty is defined as the lack of secondary sexual characteristics from the age of 13 years in females and 14 years in males. The most common cause is constitutional delay in growth and puberty. It is more common in boys and most cases are familial, usually occurring in the same-sex parent. This typically occurs in healthy children who have delayed puberty with short stature for their chronological age, but this delay is appropriate for the bone age. Pubertal delay can be classified according to circulating levels of luteinizing hormone and follicle stimulating hormone. Central nervous system abnormalities result in hypogonadotrophic hypogonadism and gonadal failure causes hypergonadotrophic hypogonadism. A detailed history is the first step in the diagnostic evaluation of a constitutional delay and abnormal puberty. Further evaluation includes laboratory testing (estradiol, testosterone, the results of a GnRH test, karyotip analysis ) and imaging studies (X-ray of the left hand and wrist, USG of the gonads, MRI ). Treatment is directed at both the acute and long-term consequences of delayed or absent pubertal development. Delayed puberty and other pubertal disorders should be recognized early, correctly diagnosed by a pediatric endocrinologist, and appropriately treated. (Turk Arch Ped 2011; 46 Suppl: 88-91)

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