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Search Results: 1 - 10 of 8426 matches for " HaiTeng Deng "
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Development of the Fc-III Tagged Protein Expression System for Protein Purification and Detection
Shan Feng, Enbing Tian, Lei Zhang, Qingtao Wang, Haiteng Deng
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044208
Abstract: In the present work, we developed the Fc-III tagged protein expression system for protein purification and detection. The Fc-III sequence encodes for a 13 residue peptide and this peptide is cyclized by disulfide bond formation when the fusion protein is expressed. The Fc-III-fusion proteins selectively bind to immunoglobulin Fc domains (IgG-Fc) expressed from E. coli. We showed the efficient purification of Fc-III tagged proteins by immobilized non-native IgG-Fc and the detection of the cellular locations of fusion proteins by fluorescent-conjugated IgG-Fc. Our results prove that Fc-III tagged protein expression system is a simple and efficient tool for protein purification and detection and is a useful addition to the biochemistry and proteomics toolbox.
Oxidative Stress Induces Monocyte Necrosis with Enrichment of Cell-Bound Albumin and Overexpression of Endoplasmic Reticulum and Mitochondrial Chaperones
Haiping Tang, Enbing Tian, Chongdong Liu, Qingtao Wang, Haiteng Deng
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0059610
Abstract: In the present study, monocytes were treated with 5-azacytidine (azacytidine), gossypol or hydrogen peroxide to induce cell death through oxidative stress. A shift from apoptotic to necrotic cell death occurred when monocytes were treated with 100 μM azacytidine for more than 12 hours. Necrotic monocytes exhibited characteristics, including enrichment of cell-bound albumin and up-regulation of endoplasmic reticulum (ER)- and mitochondrial-specific chaperones to protect mitochondrial integrity, which were not observed in other necrotic cells, including HUH-7, A2780, A549 and HOC1a. Our results show that the cell-bound albumin originates in the culture medium rather than from monocyte-derived hepatocytes, and that HSP60 is a potential binding partner of the cell-bound albumin. Proteomic analysis shows that HSP60 and protein disulfide isomerase are the most abundant up-regulated mitochondrial and ER-chaperones, and that both HSP60 and calreticulin are ubiquitinated in necrotic monocytes. In contrast, expression levels of the cytosolic chaperones HSP90 and HSP71 were down-regulated in the azacytidine-treated monocytes, concomitant with an increase in the levels of these chaperones in the cell culture medium. Collectively, our results demonstrates that chaperones from different organelles behave differently in necrotic monocytes, ER- and mitochondrial chaperones being retained and cytosolic and nuclear chaperones being released into the cell culture medium through the ruptured cell membrane. HSP60 may serve as a new target for development of myeloid leukemia treatment.
p38α MAP kinase phosphorylates RCAN1 and regulates its interaction with calcineurin
Lei Ma,HaiPing Tang,Yan Ren,HaiTeng Deng,JiaWei Wu,ZhiXin Wang
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4340-9
Abstract: RCAN1, also known as DSCR1, is an endogenous regulator of calcineurin, a serine/threonine protein phosphatase that plays a critical role in many physiological processes. In this report, we demonstrate that p38α MAP kinase can phosphorylate RCAN1 at multiple sites in vitro and show that phospho-RCAN1 is a good protein substrate for calcineurin. In addition, we found that unphosphorylated RCAN1 noncompetitively inhibits calcineurin protein phosphatase activity and that the phosphorylation of RCAN1 by p38α MAP kinase decreases the binding affinity of RCAN1 for calcineurin. These findings reveal the molecular mechanism by which p38α MAP kinase regulates the function of RCAN1/calcineurin through phosphorylation.
Structural Basis for the Regulation of Maternal Embryonic Leucine Zipper Kinase
Lu-Sha Cao, Jue Wang, Yuling Chen, Haiteng Deng, Zhi-Xin Wang, Jia-Wei Wu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070031
Abstract: MELK (maternal embryonic leucine zipper kinase), which is a member of the AMPK (AMP-activated protein kinase)-related kinase family, plays important roles in diverse cellular processes and has become a promising drug target for certain cancers. However, the regulatory mechanism of MELK remains elusive. Here, we report the crystal structure of a fragment of human MELK that contains the kinase domain and ubiquitin-associated (UBA) domain. The UBA domain tightly binds to the back of the kinase domain, which may contribute to the proper conformation and activity of the kinase domain. Interestingly, the activation segment in the kinase domain displays a unique conformation that contains an intramolecular disulfide bond. The structural and biochemical analyses unravel the molecular mechanisms for the autophosphorylation/activation of MELK and the dependence of its catalytic activity on reducing agents. Thus, our results may provide the basis for designing specific MELK inhibitors for cancer treatment.
A New Approach to the Main Problem of Subspace Coding
Haiteng Liu,Thomas Honold
Mathematics , 2014,
Abstract: Subspace codes form the appropriate mathematical setting for investigating the Koetter-Kschischang model of fault-tolerant network coding. The Main Problem of Subspace Coding asks for the determination of a subspace code of maximum size (proportional to the transmission rate) if the remaining parameters are kept fixed. We describe a new approach to finding good subspace codes, which surpasses the known size limit of lifted MRD codes and is capable of yielding an alternative construction of the currently best known binary subspace code of packet length 7, constant dimension 3 and minimum subspace distance 4.
Strategy for Identifying Dendritic Cell-Processed CD4+ T Cell Epitopes from the HIV Gag p24 Protein
Leonia Bozzacco, Haiqiang Yu, J?rn Dengjel, Christine Trumpfheller, Henry A. Zebroski, Nawei Zhang, Victoria Küttner, Beatrix M. Ueberheide, Haiteng Deng, Brian T. Chait, Ralph M. Steinman, Svetlana Mojsov, David Feny?
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041897
Abstract: Mass Spectrometry (MS) is becoming a preferred method to identify class I and class II peptides presented on major histocompability complexes (MHC) on antigen presenting cells (APC). We describe a combined computational and MS approach to identify exogenous MHC II peptides presented on mouse spleen dendritic cells (DCs). This approach enables rapid, effective screening of a large number of possible peptides by a computer-assisted strategy that utilizes the extraordinary human ability for pattern recognition. To test the efficacy of the approach, a mixture of epitope peptide mimics (mimetopes) from HIV gag p24 sequence were added exogenously to Fms-like tyrosine kinase 3 ligand (Flt3L)-mobilized splenic DCs. We identified the exogenously added peptide, VDRFYKTLRAEQASQ, and a second peptide, DRFYKLTRAEQASQ, derived from the original exogenously added 15-mer peptide. Furthermore, we demonstrated that our strategy works efficiently with HIV gag p24 protein when delivered, as vaccine protein, to Flt3L expanded mouse splenic DCs in vitro through the DEC-205 receptor. We found that the same MHC II-bound HIV gag p24 peptides, VDRFYKTLRAEQASQ and DRFYKLTRAEQASQ, were naturally processed from anti-DEC-205 HIV gag p24 protein and presented on DCs. The two identified VDRFYKTLRAEQASQ and DRFYKLTRAEQASQ MHC II-bound HIV gag p24 peptides elicited CD4+ T-cell mediated responses in vitro. Their presentation by DCs to antigen-specific T cells was inhibited by chloroquine (CQ), indicating that optimal presentation of these exogenously added peptides required uptake and vesicular trafficking in mature DCs. These results support the application of our strategy to identify and characterize peptide epitopes derived from vaccine proteins processed by DCs and thus has the potential to greatly accelerate DC-based vaccine development.
The Empirical Study on Development Value Identity and Requirement of Central Plains Folk Custom Sports Cultural Resources  [PDF]
Deng Fenglian
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B023
Abstract:

Utilizing the methods of documentary research, fieldwork, expert interviwing and so on, this paper have taken much survey in terms of cognition and value identity situation of Central Plains folk custom sports culture, the motivation of attending this activity, the development requirement trend and this culture heritage development and creation requirement trend and so on to realize the value identity and requirement of Central Plains folk custom sports cultural resources development from the public, further to wake up the attention from the public about this cultural resources which provide the culture and heritage protection, ecnomic constructure and culture constrcture with theoretical references and practical instruction.

A Note on a Combinatorial Conjecture  [PDF]
Guixin Deng
Open Journal of Discrete Mathematics (OJDM) , 2013, DOI: 10.4236/ojdm.2013.31010
Abstract: It is difficult to find Boolean functions achieving many good cryptographic properties. Recently, Tu and Deng obtained two classes of Boolean functions with good properties based on a combinatorial conjecture about binary strings. In this paper, using different approaches, we prove this conjecture is true in some cases. This conjecture has resisted different attempts of proof since it is hard to find a recursive method. In this paper we give a recursive formula in a special case.
The Herd Behavior of Risk-Averse Investor Based on Information Cost  [PDF]
Guangming Deng
Journal of Financial Risk Management (JFRM) , 2013, DOI: 10.4236/jfrm.2013.24015
Abstract: In this paper, the traditional model of herd behavior was improved and extended. The herd behavior of risk-averse investor based on information cost was studied in the financial market. By refining the concept of Bayes equilibrium and the analysis of the behavior of investors, it was discovered that the herd behavior of the second risk-averse investor did not produce until the first risk-averse investors chose to buy information.
Why “Ding-Dong Community” Cannot Succeed Like American Version “Nextdoor”?  [PDF]
Pei Deng
Journal of Service Science and Management (JSSM) , 2015, DOI: 10.4236/jssm.2015.84065
Abstract: The conception of community O2O is very popular currently in China, which has become the focus of attention in the field of e-commerce. However, since at the preliminary exploration stage everything in this area changes very quickly, there is not yet mature business model to mimic. Many of the problems should be studied and clarified. Taking the rise and failure of “ding-dong district” as a case, this article attempts to analyze the unsuccessful reason that “ding-dong district” imitates the US version “Nextdoor” in the context of China, and put forward the corresponding countermeasures and suggestions, aiming to provide related reference for Chinese enterprises in the exploration and development of community e-commerce.
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