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Search Results: 1 - 10 of 74983 matches for " HU Yan-jie "
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Visible-light Photocataltic Activity of Cr-doped TiO2 Nanoparticles Synthesized by Flame Spray Pyrolysis
TIAN Bao-Zhu,LI Chun-Zhong,GU Feng,JIANG Hai-Bo,HU Yan-Jie
无机材料学报 , 2009, DOI: 10.3724/sp.j.1077.2009.00661
Abstract: Cr-doped TiO2 nanoparticles were synthesized by one-step flame spray pyrolysis technique. The effects of Cr3+ doping on the microstructure, light absorption property, and photocatalytic activity of the samples were investigated. It is found that increasing Cr3+ concentration restrains the formation of anatase and simultaneously favors the formation of rutile. At low concentration ( %), Cr is mainly incorporated into the crystal lattice of TiO2 in the form of Cr3+, while high Cr concentration promotes the formation of Cr2O3 clusters. The result of photocatalytic degradation of 2,4-dichlorophenol demonstrates that appropriate Cr3+ doping can evidently enhance the visible-light photocatalytic activity of TiO2. The optimal Cr3+ concentration to obtain the highest photocatalytic activity is 1at%. The improvement of visible-light photocatalytic activity derived from Cr doping is mainly related to the enhancement of visible light absorption, appropriate crystalline composition, and improved transfer efficiency of photogenerated electrons and holes.
Mechanism Analysis and Preparation of Core-shell TiO2/SiO2 Nanoparticles by H2/Air Flame Combustions
HU Yan-Jie,LI Chun-Zhong,Cong De-Zi,JIANG Hai-Bo,ZHAO Yin
无机材料学报 , 2007, DOI: 10.3724/sp.j.1077.2007.00205
Abstract: Core-shell TiO2/SiO2 nanoparticles were synthesized successfully by H2/Air flame combustions, and the formation mechanism of core-shell nanoparticles was analyzed. The structures and properties of these TiO2/SiO2 particles were investigated by using TEM, HRTEM, XRD and FTIR. The transformation from anatase to rutile and the grain growth are hindered due to the addition of silica into matrix. Both the chemical reaction rate and the nucleation rate of TiO2 particles are much faster than those of SiO2. The formation of primary TiO2 particles is faster than that of SiO2 in the third jet due to the low temperature and nuclear rate. Heterogeneous nucleaton of SiO2 on the surface of TiO2 is the main reason of core-shell nanocomposites formation.
Crystal Structures of the Transcriptional Repressor RolR Reveals a Novel Recognition Mechanism between Inducer and Regulator
De-Feng Li,Ning Zhang,Yan-Jie Hou,Yan Huang,Yonglin Hu,Ying Zhang,Shuang-Jiang Liu,Da-Cheng Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019529
Abstract: Many members of the TetR family control the transcription of genes involved in multidrug resistance and pathogenicity. RolR (Resorcinol Regulator), the recently reported TetR-type regulator for aromatic catabolism from Corynebacterium glutamicum, distinguishes itself by low sequence similarities and different regulation from the previously known members of the TetR family. Here we report the crystal structures of RolR in its effector-bound (with resorcinol) and aop- forms at 2.5 ? and 3.6 ?, respectively. The structure of resorcinol-RolR complex reveal that the hydrogen-bonded network mediated by the four-residue motif (Asp94- Arg145- Arg148- Asp149) with two water molecules and the hydrophobic interaction via five residues (Phe107, Leu111, Leu114, Leu142, and Phe172) are the key factors for the recognition and binding between the resorcinol and RolR molecules. The center-to-center separation of the recognition helices h3-h3′ is decreased upon effector-binding from 34.9 ? to 30.4 ?. This structural change results in that RolR was unsuitable for DNA binding. Those observations are distinct from that in other TetR members. Structure-based mutagenesis on RolR was carried out and the results confirmed the critical roles of the above mentioned residues for effector-binding specificity and affinity. Similar sequence searches and sequence alignments identified 29 RolR homologues from GenBank, and all the above mentioned residues are highly conserved in the homologues. Based on these structural and other functional investigations, it is proposed that RolR may represent a new subfamily of TetR proteins that are invovled in aromatic degradation and sharing common recognition mode as for RolR.
Molecular mechanism of the integration and lysis of mycobacteriophage

SHEN Yan-jie,HU Chang-hua,WANG Hong-hai,XIE Jian-ping,

微生物学报 , 2005,
Abstract: Tuberculosis remains one of the major threats to public health. China is one of the heavy TB burden countries. Novel drugs and vaccines are urgently needed to combat the increasingly multidrug resistant TB. Mycobacteriophage is one of the hot topic in TB novel drugs discovery and drug susceptibility test. Phages can multiply via two alternative mechanisms: the lytic cycle or the lysogenic cycle. The lytic cycle ends with the lysis and death of the host cell, whereas the host cell remains alive in the lysogenic cycle. Lysogenic mycobacteriophages were intensively studied to elucidate the integration and lysis mechanisms of mycobacteriophage. The integration of mycobacteriophage requires for attP of bacteriopahge genome, attB of Mycobacterium genome, integrase and integration host factor. Some lysogenic phage, eg. mycobacteriophage Ms6,employ lytic cycle, form new phage, lysis host by the cooperation of lysin and holin, and release phages. There is no reports as to the mycobacteriophage unique to China clinical or environmental isolates. Studies on the integration and lysis molecular mechanism of mycobacteriophage might facilitate future new anti-TB drugs development.
Visible-light Photocataltic Activity of Cr-doped TiO2 Nanoparticles Synthesized by Flame Spray Pyrolysis

TIAN Bao-Zhu,LI Chun-Zhong,GU Feng,JIANG Hai-Bo,HU Yan-Jie,

无机材料学报 , 2009,
Abstract: 采用一步喷雾燃烧热解法制备了Cr掺杂TiO2纳米粒子,研究了Cr掺杂对样品微结构、吸光特性和可见光催化活性的影响. 结果表明:增加Cr掺杂量抑制锐钛矿相的形成,同时促进金红石相的形成. 在低Cr掺杂量下(≤1%),Cr主要以Cr3+的形态进入TiO2晶格,而Cr掺杂量过大时,易于形成Cr2O3团簇. 光催化降解2,4-二氯苯酚结果表明,适量的Cr3+掺杂可以有效地提高TiO2的可见光催化活性,获得最高光催化活性的Cr3+掺杂量为1at%. 样品可见光催化活性的提高主要与Cr掺杂引起的可见光吸收增强、晶相组成改善以及光生电子和空穴传输效率提高有关.
Flame Spray Synthesis of SnO2 Nanorods and Their Gas Sensing Properties

WU Zhi-wen,HU Yan-jie,LI Chun-zhong,

过程工程学报 , 2012,
Abstract: SnO2 nanorods were synthesized by flame spray pyrolysis. Their morphology and structure were characterized. The as-prepared SnO2 nanorods grow along the (001) direction with the length of 200~350 nm and diameter of 30~50 nm. The effects of Fe doping and Sn4+ concentration on their morphology and growth mechanism were investigated. In the high temperature and rapid reaction process the Fe3+ could permeate in SnO2 crystals and promote their growth along the (001) direction. The gas sensing performance tests showed that the SnO2 nanorods exhibited more excellent gas sensing properties than SnO2 nanoparticles with a sensitivity of (12~100)×10-6 (ψ) ethanol as well as response and recovery times of 9.5 and 6 s, respectively.
Preparation of Nano-Al2O3 Particles via H2/Air Flame Synthesis and Their Dispersion Performance

DING Hong-qiu,HU Yan-jie,LI Chun-zhong,

过程工程学报 , 2011,
Abstract: 利用多重射流氢氧燃烧反应器,以AlCl3为前驱体制备了具有不同形貌和晶型结构的Al2O3纳米颗粒,表征了其形貌、晶型结构、比表面积、粒径分布等性能,考察了火焰燃烧形式和反应区最高温度等因素对颗粒性能的影响规律. 结果表明,随反应温度升高,Al2O3粒径不断长大,形貌从具有链状结构的不规则颗粒逐渐转变为分散性良好的球形颗粒;同时随反应温度升高和在高温火焰中停留时间延长,晶型由单纯g相逐渐转变为d和d*相. 产品纳米Al2O3颗粒具有较强亲水性,其分散液具有较好的稳定性. 探讨了高温快速反应过程中颗粒和团聚体的生长机理,最终产物的粒径和团聚体形貌取决于各主要影响条件的相互竞争.
Effects of Applied Potential on the Growth Process of Porous Aluminum Oxide Membrane

JIANG Hai-bo,LI Chun-zhong,ZHAO Yin,HU Yan-jie,

过程工程学报 , 2007,
Abstract: Two-step electrochemical anodizing approach was introduced to prepare the porous aluminum oxide(AAO)membrane in oxalic acid solution.The dependence of morphology and growth process of AAO membrane on applied voltage was studied.It is found that current density,growth rate,pore diameters and pore distances of AAO membrane increase with the anodizing voltage increasing and the volume expansion factor varies linearly with the anodizing voltage.The porosity keeps a constant of 12%,which is independent of the applied voltage.
Isolation and antifungal activity of a biosurfactant-producing bacterium

WANG Yan-Jie,BI Si-Ning,ZUO Yu-Hu,HU Xiao-Ping,KANG Zhen-Sheng,WANG Yan-Jie,HUANG Xiao-Dong,

微生物学通报 , 2012,
Abstract: Objective] The objective of this study was to examine the production of biosurfactant, taxonomic position and antifungal activity of the strain BS1. Methods] Biosurfactant-producing bacterium was isolated by hemolytic activity assay on blood agar plates and hydrolyzing oil activity estimation on oil agar plates. Oil spreading method was used to assay surface activity. The strain BS1 was indentified according to morphological features, physiological and biochemical characteristics and 16s rDNA sequences of the strain. Antifungal activity was tested by dual-culture method and the inhibiting effect on the the mycelium growth, sporagium formation and spore germination. Results] The strain BS1 of producing biosurfactant, isolated from the petroleum-polluted soil, was indentified as Pseudomonas sp.. The strain BS1, fermentation supernatant and the volatile compounds (VCs) exhibited inhibition activity on 12 kinds of plant pathogenic fungis. The strain BS1 and fermentation supernatant displayed significant inhibition to the Phytophthora sojae, the inhibitory rate was 65.31% and 95.93%, respectively. The fermentation supernatant of BS1 inhibited the growth of Phytophthora sojae by inhibiting mycelium growth, sporagium formation and spore germination, which had also remarkable inhibition even if it was diluted 20 times with pure water. VCs produced by strain BS1 had notable inhibiting effect against Sclerotinia sclerotiorum, and its inhibitory rate was 84.25%. Conclusion] The strain BS1 can produce surfactant and has the potential biocontrol.
Circulating microRNAs as a Fingerprint for Liver Cirrhosis
Yan-Jie Chen, Ji-Min Zhu, Hao Wu, Jia Fan, Jian Zhou, Jie Hu, Qian Yu, Tao-Tao Liu, Lei Yang, Chun-Lei Wu, Xiao-Ling Guo, Xiao-Wu Huang, Xi-Zhong Shen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066577
Abstract: Background Sensitive and specific detection of liver cirrhosis is an urgent need for optimal individualized management of disease activity. Substantial studies have identified circulation miRNAs as biomarkers for diverse diseases including chronic liver diseases. In this study, we investigated the plasma miRNA signature to serve as a potential diagnostic biomarker for silent liver cirrhosis. Methods A genome-wide miRNA microarray was first performed in 80 plasma specimens. Six candidate miRNAs were selected and then trained in CHB-related cirrhosis and controls by qPCR. A classifier, miR-106b and miR-181b, was validated finally in two independent cohorts including CHB-related silent cirrhosis and controls, as well as non?CHB-related cirrhosis and controls as validation sets, respectively. Results A profile of 2 miRNAs (miR-106b and miR-181b) was identified as liver cirrhosis biomarkers irrespective of etiology. The classifier constructed by the two miRNAs provided a high diagnostic accuracy for cirrhosis (AUC = 0.882 for CHB-related cirrhosis in the training set, 0.774 for CHB-related silent cirrhosis in one validation set, and 0.915 for non?CHB-related cirrhosis in another validation set). Conclusion Our study demonstrated that the combined detection of miR-106b and miR-181b has a considerable clinical value to diagnose patients with liver cirrhosis, especially those at early stage.
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