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Search Results: 1 - 10 of 47600 matches for " HU Meng-Yao "
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Variations in leaf functional traits of Stipa purpurea along a rainfall gradient in Xizang,China

HU Meng-Yao,ZHANG Lin,LUO Tian-Xiang,SHEN Wei,

植物生态学报 , 2012,
Abstract: Aims Stipa purpurea is the dominant species in alpine arid and semi-arid grasslands on the Tibetan Plateau. Our objectives are to determine if this species exhibits a strategy shift in its specific leaf area (SLA) to nitrogen (N) concentration relationship along a rainfall gradient and to detect possible effects of environmental factors on related leaf traits. Methods We investigated variations in leaf traits of S. purpurea associated with climatic and soil factors along an east-west transect with a rainfall gradient (69–479 mm) but similar altitudes (4 300–4 700 m). Five locations from east to west are Damxung, Namco, Gêrzê, Mount Qomolangma and Rutog. We measured SLA, mass- and area-based leaf N concentration (Nmass, Narea), leaf density and thickness and soil total N along the transect. Important findings In pooled data, SLA and Nmass varied little with the growing season mean temperature and precipitation and the soil total N concentration. The SLA-Nmass relationship in S. purpurea did not shift between the semi-humid areas (ratio of rainfall to evaporation > 0.11) and the arid and semi-arid areas (ratio < 0.11), although there was a positive correlation between SLA and Nmass across the five locations. Variation in SLA was mainly determined by leaf density in the semi-humid areas and by leaf thickness in the arid and semi-arid areas; both were negatively correlated with SLA. With increasing temperature or declining precipitation, leaf density decreased and leaf thickness increased, leading to non-significant relationships between SLA and climatic factors. The increase of leaf density in the semi-humid areas was correlated with the increase of Narea, but the increase of leaf thickness in the arid and semi-arid areas did not lead to change of Narea, resulting in unchanged Narea along the rainfall gradient. A positive correlation was detected between aboveground biomass and Narea in S. purpurea, indicating that increased Narea may increase plant productivity. Our findings suggest that alpine plants in arid and semi-arid areas may maintain a constant Narea by increased leaf thickness in order to achieve a similar photosynthetic productivity and water use efficiency compared to the relatively humid areas. The relative impacts of leaf density and leaf thickness on SLA shifted between the semi-humid areas and the arid and semi-arid areas, which may provide insight in detecting the threshold of water limitation in alpine grasslands.
Qi-Ping ZHU,Meng-Yao ZHU,Ying-Chao HU,Xue-Ya ZHANG,Guo-Hua DING,Zhi-Hua LIN
动物学研究 , 2015, DOI: 10.13918/j.issn.2095-8137.2015.1.29
Abstract: Inreptiles,habitatselectionistheprocesswherebysuitablehabitatisselectedthatoptimizesphysiologicalfunctionsandbehavioralperformance.Here,weusedthebrownforestskink(Sphenomorphusindicus)asamodelanimalandexaminedwhetherthefrequencyofactiveindividuals,environmentaltemperature,illuminationofactivityarea,andhabitattypevarywithdifferentageclasses.WesurveyedthenumberofactiveindividualsandmeasuredenvironmentalvariablesatBaiyunshanMountaininLishui,Zhejiang,China.WefoundnodifferenceintheactivityfrequencyofadultandjuvenileS.indicus;theactivitypatternofactiveindividualswasbimodal.Themeanenvironmentaltemperatureselectedbyadultswashigherthanthatselectedbyjuveniles.Theenvironmentaltemperatureofactiveareasmeasuredat0900-1000hand1100-1200hwashigherthanat1400-1500h;illuminationoftheactiveareaat1000-1200hwasalsohigherthanat1400h-1600h.Thenumberofactiveindividuals,theenvironmentaltemperatureandilluminationofactivityareasshowedpairwisepositivecorrelation.Therewasadifferenceinhabitattypebetweenjuvenilesandadultswherebyjuvenilespreferrockhabitats.WepredictthatactiveS.indicusselectoptimalhabitatswithdifferentenvironmentaltemperaturesandtypestoreachthephysiologicalneedsparticulartotheirageclasses.
Identification of a constitutional mutation in the WT1 gene in Taiwanese patients with Wilms tumor  [PDF]
Meng-Yao Lu, Wen-Chung Wang, Chiao-Wen Lin, Alice Chang, Yen-Chein Lai
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.53029

The overall frequency of WT1 gene alterations in Wilms tumor is still unclear in Taiwan. Here we conducted molecular genetic analysis of the WT1 gene in Taiwanese patients with Wilms tumor. Polymerase chain reaction and direct sequencing were performed on DNA samples from blood and paraffin-embedded tumor specimens. A constitutional mutation in the WT1 gene was found in one DNA sample from peripheral blood lymphocytes. The remaining DNA samples from peripheral blood lymphocytes and paraffin-embedded tumor specimens were tested negative for both constitutional mutations and somatic mutations. Thus, mutations at other Wilms tumor loci may play an important role in Wilms tumor development.

Identification of Intrauterine Mycoplasma in Endometritis of Dairy Cow Isolates by 16S rDNA Nested PCR-DGGE
Meng-Yao Guo,Guo-Qing Wang,Guoxi Shao,Wulin Tian,Yuanjing Xu,Guanghong Xie
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.1745.1752
Abstract: Molecular diagnostics methods are used to investigate mycoplasma infection status but this approach to infection analysis of mycoplasma in endometritis of dairy cow has not been reported. In this study, a nest Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (nested PCR-DGGE) assay for detection of 16S ribosomal RNA of mycoplasma was used as reference of 4 isolates which were identified in a previous survey of dairy cow s reproductive system in Jilin province, China. A reference PCR-DGGE profile was generated using DNA extracted and amplified from the strain of these cultures. The effectiveness of the procedure was assessed by application to test the samples from endometritis dairy cow s uterus mucus. The bands from the nested PCR-DGGE were extracted for sequencing.
(1S,4S,5S,8R)-8-Nitrooxy-2,6-dioxabicyclo[3.3.0]octan-4-yl 3,4,5-triacetoxybenzoate
Yue Zhang,Ping-An Wang,Meng-Yao Zhang,Xiao-Li Sun
Acta Crystallographica Section E , 2009, DOI: 10.1107/s1600536809042524
Abstract: In the title compound, C19H19NO13, one of the two fused furanose rings adopts an envelope conformation whereas the other displays a twisted conformation. The crystal structure is stabilized by intermolecular C—H...π interactions between a methine H atom and the triacetoxyphenyl ring of an adjacent molecule, and by weak non-classical intermolecular C—H...O hydrogen bonds.
Tumor suppressor function of ezrin-radixin-moesin-binding phosphoprotein-50 through β-catenin/E-cadher in pathway in human hepatocellular cancer
Xiu-Lan Peng,Meng-Yao Ji,Zi-Rong Yang,Jia Song
World Journal of Gastroenterology , 2013, DOI: 10.3748/wjg.v19.i8.1306
Abstract: AIM: To determine the effect and molecular mechanism of ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) in hepatocellular carcinoma (HCC). METHODS: Three human HCC cell lines, i.e., SM-MC7721, HepG2 and Hep3B, were used. We transfected the Pbk-CMV-HA-EBP50 plasmid into SMMC7721 cells with Lipofectamine 2000 to overexpress EBP50. Western blotting were performed to determine the effects of the plasmid on EBP50 expression and to detect the expression of β-catenin and E-cadherin before and after the transfection of the plasmid into SMMC7721 cells. In vitro cell proliferation was assessed with a Cell Counting Kit-8 (CCK-8) assay. Cell cycle distribution was assessed with flow cytometry. Invasion and migration ability of before and after the transfection were determined with a transwell assay. Cell apoptosis was demonstrated with Annexin V-FITC. The effect of EBP50 overexpressing on tumor growth in vivo was performed with a xenograft tumor model in nude mice. RESULTS: The transfection efficiency was confirmed with Western blotting (1.36 ± 0.07 vs 0.81 ± 0.09, P < 0.01). The CCK8 assay demonstrated that the growth of cells overexpressing EBP50 was significantly lower than control cells (P < 0.01). Cell cycle distribution showed there was a G0/G1 cell cycle arrest in cells overexpressing EBP50 (61.3% ± 3.1% vs 54.0% ± 2.4%, P < 0.05). The transwell assay showed that cell invasion and migration were significantly inhibited in cells overexpressing EBP50 compared with control cells (5.8 ± 0.8 vs 21.6 ± 1.3, P < 0.01). Annexin V-FITC revealed that apoptosis was significantly increased in cells overexpressing EBP50 compared with control cells (14.8% ± 2.7% vs 3.4% ± 1.3%, P < 0.05). The expression of β-catenin was downregulated and E-cadherin was upregulated in cells overexpressing EBP50 compared with control cells (0.28 ± 0.07 vs 0.56 ± 0.12, P < 0.05; 0.55 ± 0.08 vs 0.39 ± 0.07, P < 0.05). In vivo tumor growth assay con rmed that up-regulation of EBP50 could obviously slow the growth of HCC derived from SMMC7721 cells (28.9 ± 7.2 vs 70.1 ± 7.2, P < 0.01). CONCLUSION: The overexpression of EBP50 could inhibit the growth of SMMC7721 cells and promote apoptosis by modulating β-catenin, E-cadherin. EBP50 may serve asa potential therapeutic target in HCC.
Threshold property of a nematic liquid crystal cell with two grating surface substrates

Ye Wen-Jiang,Xing Hong-Yu,Yang Guo-Chen,Yuan Meng-Yao,

中国物理 B , 2009,
Selection of Suitable Reference Genes for qPCR Normalization under Abiotic Stresses in Oenanthe javanica (BI.) DC
Qian Jiang, Feng Wang, Meng-Yao Li, Jing Ma, Guo-Fei Tan, Ai-Sheng Xiong
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0092262
Abstract: Accurate normalization of gene expression data is an absolute prerequisite to obtain reliable results in qPCR analysis. Oenanthe javanica, an aquatic perennial herb, belongs to the Oenanthe genus in Apiaceae family, with known medicinal properties. In the current study, O. javanica was subjected to hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid) and abiotic stresses (heat, cold, salt, and drought), and the expression of nine candidate reference genes (eIF-4α, ACT7, TIP41, GAPDH, SAND, EF-1α, PP2A, TBP, and TUB) was evaluated. Stability of the genes was assessed using geNorm, NormFinder and BestKeeper. All the genes presented distinct expression profiles under the experimental conditions analyzed. Under abiotic stress conditions, ACT7 and PP2A genes displayed the maximum stability; PP2A and SAND were the most stable genes under hormone stimuli. Even though PP2A gene was most stable across all the samples, individual analysis revealed changes in expression profile. To further validate the suitability of the reference genes identified in this study, the expression level of M6PR gene under salt treatment was studied. Based on our data, we propose that it is essential to normalize the target gene expression with specific reference genes under different experimental conditions for most accurate results. To our knowledge, this is the first systematic analysis for reference genes under abiotic stress and hormone stimuli conditions in O. javanica. This will be beneficial for future studies on O. javanica and other plants in Apiaceae family at molecular level.
Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica
Chao-an Su, Xiao-yan Xu, De-yun Liu, Ming Wu, Fan-qing Zeng, Meng-yao Zeng, Wei Wei, Nan Jiang, Xia Luo
DARU Journal of Pharmaceutical Sciences , 2013, DOI: 10.1186/2008-2231-21-5
Abstract: A water-soluble Morchella conica Polysaccharides (MCP) were extracted and isolated from the fermentation broth of M. conica through a combination of DEAE-cellulose and Sephacryl S-300 HR chromatograph. NMR and IR spectroscopy has played a developing role in identification of polysaccharide with different structure and composition from fungal and plant sources, as well as complex glycosaminoglycans of animal origin. Thus, NMR and IR spectroscopy were used to analyze the chemical structure and composition of the isolated polysaccharide. Moreover, the polysaccharide was tested for its immunomodulatory activity at different concentrations using in vitro model.The results showed that MCP may significantly modulate nitric oxide production in macrophages, and promote splenocytes proliferation. Analysis from HPLC, infrared spectra and nuclear magnetic resonance spectroscopy showed that MCP was a homogeneous mannan with an average molecular weight of approximately 81.2 kDa. The glycosidic bond links is [rightwards arrow]6)-alpha-D-Man p-(1[rightwards arrow].The results suggested that the extracted MCP may modulate nitric oxide production in macrophages and promote splenocytes proliferation, and it may act as a potent immunomodulatory agent.
Theoretical study on phase conjugation in weakly injected vertical-cavity surface-emitting lasers

Zhang Wei-Li,Pan Wei,Luo Bin,Li Xiao-Feng,Zou Xi-Hua,Wang Meng-Yao,

中国物理 B , 2008,
Abstract: This paper gives a detailed theoretical investigation on phase conjugation induced by nearly degenerate four-wave mixing in single mode vertical-cavity surface-emitting lasers (VCSELs) with weak optical injection. Considering VCSELs that can work in linearly polarized or elliptically polarized states, it derives the theoretical expression of the conjugated field by small signal analysis based on the vectoral rate equations---the spin-flip model. For linearly polarized state, VCSELs show similar conjugate spectra to edge-emitting semiconductor lasers. For the elliptically polarized state, dichroism and birefringence parameters as well as the spin-flip rate can change the conjugate spectra. Especially, when frequency detuning of the probe and pump waves is between the positive and negative relaxation oscillation frequency, changes are evident. For specific values of parameters, conjugate efficiency between 20\,dB to 40dB are obtained.
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