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Search Results: 1 - 10 of 677220 matches for " H. Mejía-López "
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Caracterización genética de adenovirus aislados de conjuntivitis folicular y queratoconjuntivitis epidémica en un grupo de pacientes mexicanos
Mejía-López,H.; Santacruz-Valdés,C.; Matías-Florentino,M.;
Archivos de la Sociedad Espa?ola de Oftalmología , 2008, DOI: 10.4321/S0365-66912008000300006
Abstract: objetive: to determine the adenovirus serotype in mexican patients with folicular conjunctivitis and keratoconjunctivitis. methods: adenovirus-specific pcr was used to analyze sample scrapings from the inferior fornix of patients with follicular conjunctivitis and clinical suspicion of adenovirus from january 2005 to december 2006. identification of the serotype was made by automated sequencing. the nucleotide sequences obtained were compared with the reported sequences in genbank. descriptive statistical analyses were performed on the results. results: of the 77 samples with clinical data of follicular conjunctivitis that were analyzed, 43 (56%) presented adenovirus. the sequencing of each positive sample allowed the identification of ad1, ad2, ad3 and ad8; the sequences of the serotype were identical those reported in genbank with accession numbers: af 534906 and ay 224420 for a sequence of the gene coding for the filament of ad1 and ad2 respectively, and ay 854180 and dq 149614 for a sequence of the gene that codes for the hexon protein of ad3 and ad8 respectively. from the statistical analysis it was possible to determine that a preferential seasonality of the serotype does not exist. conclusion: in this work the ad1, ad2 and ad3 serotypes were identified in patients with clinical diagnosis of follicular conjunctivitis in 2005. ad2 was the predominant serotype. ad8 was also detected in an outbreak of epidemic keratoconjunctivitis. from an epidemiological point of view, no serotype found seems to have a preferred seasonality.
Identificación por métodos moleculares de adenovirus asociados a conjuntivitis
Mejía-López,H.; Matías-Florentino,M.; Vélez-Montoya,R.;
Archivos de la Sociedad Espa?ola de Oftalmología , 2006, DOI: 10.4321/S0365-66912006000700005
Abstract: objective: to identify adenovirus in patients with conjunctivitis by molecular methods such as the polymerase chain reaction (pcr) and dna sequencing. methods: samples of scrapings from the inferior fornix of 51 patients (39 diagnosed with follicular conjunctivitis and 12 diagnosed with vernal conjunctivitis) were processed by generic pcr to identify adenovirus. all the samples that were pcr positive were cultured on vero cells for virus isolation, with this being demonstrated by immunofluorescence. for the identification of the isolated serotype, the multiplex pcr was utilized and dna automated sequencing was employed to identify the genetic variants. results: twenty-eight of the individuals diagnosed with follicular conjunctivitis and six of those diagnosed with vernal conjunctivitis, had positive results to adenovirus (67%). the cultures in vero cells allowed the isolation of eight samples. only three of the isolated viruses (one ad1 and two ad2) were identified by the multiplex pcr used to identify the subgenus c adenovirus. an ad1 genetic variant was identified by automated sequencing while the ad2 serotypes were identical to the ones reported by genbank. conclusions: the polymerase chain reaction and dna sequencing are useful tools to identify and characterize microorganisms responsible for diseases such as conjunctivitis caused by adenoviruses.
Caracterización genética de adenovirus aislados de conjuntivitis folicular y queratoconjuntivitis epidémica en un grupo de pacientes mexicanos Genetic characterization of adenovirus isolated from follicular conjunctivitis and epidemic keratoconjuntivitis in a group of mexican patients
H. Mejía-López,C. Santacruz-Valdés,M. Matías-Florentino
Archivos de la Sociedad Espa?ola de Oftalmología , 2008,
Abstract: Objetivo: Conocer los serotipos de adenovirus en pacientes mexicanos con conjuntivitis folicular y queratoconjuntivitis. Métodos: Se analizaron por PCR específica para adenovirus, muestras de fondo de saco conjuntival inferior de 77 pacientes diagnosticados con conjuntivitis folicular por sospecha clínica de infección por adenovirus, de enero de 2005 a diciembre de 2006. La identificación de los serotipos se realizó por secuenciación automatizada. Las secuencias de nucleótidos obtenidos fueron comparados con las secuencias reportadas en el GenBank. En el análisis de los resultados se utilizó la estadística descriptiva. Resultados: Se analizaron 77 muestras de las cuales el 56% presentaron adenovirus. La secuenciación de cada muestra positiva permitió la identificación de Ad1, Ad2, Ad3 y Ad8; las secuencias de los serotipos fueron idénticas a las reportadas en el GenBank con las direcciones: AF 534906 y AY 224420 para una secuencia del gen que codifica el filamento de Ad1 y Ad2 respectivamente y AY 854180 y DQ 149614 para una secuencia del gen que codifica la proteína Hexón de Ad3 y Ad8 respectivamente. En el análisis estadístico, se pudo observar que no existe, en apariencia, una estacionalidad preferencial de los serotipos identificados. Conclusiones: En este trabajo se identificaron los serotipos Ad1, Ad2 y Ad3 en pacientes con diagnóstico clínico de conjuntivitis folicular en el 2005, Ad2 fue el serotipo predominante. También se detectó en un brote de queratoconjuntivitis epidémica por Ad8. Desde el punto de vista epidemiológico, ningún serotipo encontrado parece tener estacionalidad preferente. Objetive: To determine the adenovirus serotype in Mexican patients with folicular conjunctivitis and keratoconjunctivitis. Methods: Adenovirus-specific PCR was used to analyze sample scrapings from the inferior fornix of patients with follicular conjunctivitis and clinical suspicion of adenovirus from January 2005 to December 2006. Identification of the serotype was made by automated sequencing. The nucleotide sequences obtained were compared with the reported sequences in GenBank. Descriptive statistical analyses were performed on the results. Results: Of the 77 samples with clinical data of follicular conjunctivitis that were analyzed, 43 (56%) presented adenovirus. The sequencing of each positive sample allowed the identification of Ad1, Ad2, Ad3 and Ad8; the sequences of the serotype were identical those reported in GenBank with accession numbers: AF 534906 and AY 224420 for a sequence of the gene coding for the filament of Ad1 and Ad2 respectively, and A
Identificación por métodos moleculares de adenovirus asociados a conjuntivitis Identification of adenovirus associated with conjunctivitis by molecular methodology
H. Mejía-López,M. Matías-Florentino,R. Vélez-Montoya
Archivos de la Sociedad Espa?ola de Oftalmología , 2006,
Abstract: Objetivo: Por medio de métodos moleculares como la Reacción en Cadena de la Polimerasa (PCR) y la secuenciación del material genético, identificar adenovirus en pacientes con conjuntivitis. Métodos: Se procesaron muestras de raspado del saco conjuntival inferior de 51 pacientes (39 diagnosticados con conjuntivitis folicular y 12 diagnosticados con conjuntivitis vernal) para identificar adenovirus por medio de la PCR genérica. Todas las muestras positivas en la PCR genérica, fueron cultivadas en células VERO para el aislamiento del virus, éste se demostró por inmunofluorescencia. Se utilizó la PCR múltiple para la caracterización de los serotipos aislados y las variantes genéticas se identificaron mediante la secuenciación automatizada. Resultados: Veintiocho de los pacientes diagnosticados con conjuntivitis folicular y seis de los diagnosticados con conjuntivitis vernal resultaron positivos a adenovirus (67%). El cultivo en células VERO permitió el aislamiento del virus de 8 muestras. Sólo tres de los aislados fueron identificados (un serotipo Ad1 y dos Ad2) por la PCR múltiple que identifica adenovirus del subgénero C. Por secuenciación automatizada se identificó una variante genética correspondiente al Ad1, mientras que los aislados de Ad2 fueron idénticos a los reportados en el Banco de Genes. Conclusiones: La Reacción en Cadena de la Polimerasa y la Secuenciación son herramientas útiles para la identificación y caracterización de agentes causantes de enfermedades tales como la Conjuntivitis por Adenovirus. Objective: To identify adenovirus in patients with conjunctivitis by molecular methods such as the Polymerase Chain Reaction (PCR) and DNA sequencing. Methods: Samples of scrapings from the inferior fornix of 51 patients (39 diagnosed with Follicular Conjunctivitis and 12 diagnosed with Vernal Conjunctivitis) were processed by generic PCR to identify adenovirus. All the samples that were PCR positive were cultured on VERO cells for virus isolation, with this being demonstrated by immunofluorescence. For the identification of the isolated serotype, the multiplex PCR was utilized and DNA automated sequencing was employed to identify the genetic variants. Results: Twenty-eight of the individuals diagnosed with Follicular Conjunctivitis and six of those diagnosed with Vernal Conjunctivitis, had positive results to adenovirus (67%). The cultures in VERO cells allowed the isolation of eight samples. Only three of the isolated viruses (one Ad1 and two Ad2) were identified by the multiplex PCR used to identify the subgenus C adenovirus. An Ad1 genetic var
Asymmetric magnetic dots: A way to control magnetic properties
N. M. Vargas,S. Allende,B. Leighton,J. Escrig,J. Mejía-López,D. Altbir,Ivan K. Schuller
Physics , 2011, DOI: 10.1063/1.3561483
Abstract: We have used Monte Carlo simulations to investigate the magnetic properties of asymmetric dots as a function of their geometry. The asymmetry of round dots is produced by cutting off a fraction of the dot and is characterized by an asymmetry parameter $\alpha$. This shape asymmetry has interesting effects on the coercivity ($H_{c}$), remanence ($M_{r}$), and barrier for vortex and C- state formation. The dependences of $H_{c}$ and $M_{r}$ are non monotonic as a function of $\alpha$ with a well defined minima in these parameters. The vortex enters the most asymmetric part and exits through the symmetric portion of the dot. With increasing $\alpha $ the vortex formation starts with a C-state which persists for longer fields and the barrier for vortex exit diminishes with increasing asymmetry, thus providing control over the magnetic chirality. This implies interesting, naively-unexpected, magnetic behavior as a function of geometry and magnetic field.
Phytoclimatic Dynamics of Mediterranean Forests under Climate Change. A Case Study in a Southern European Pinus sylvestris L. Stand  [PDF]
Javier M. García-López, Carmen Allué
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43A084
Abstract:

Some effects of climate change on the composition and competitive capacity of southern European Pinus sylvestris L. forests in the Mediterranean basin were evaluated. The variation over the period 1910-2008 through 30-year mobile averages of a Phytoclimatic Suitability Index (PSI) of the main tree species of the forest cover are used to indicate the competitive hierarchy of Pinus sylvestris and Fagus sylvatica L. The methodology was applied at a specific location on the Spanish south-facing slopes of the Pyrenees mountain range in the Iberian Peninsula, where the increase in the average temperature was 1.4?C in the period of observation. The results indicated that the apparent equilibrium between the two species studied changed from the 1934-1963 average. Due to the loss of competitive capacity of Scots pine with respect to European beech, particularly from the years 1970-1999, the model predicted an inversion of the situation as it was up until now, so that beech had a higher PSI than pine. The phytoclimatic approach proposed here offers new methodological horizons for the study of the effects of climate change on the future of the forests.

?Qué nos dicen las observaciones cosmológicas sobre el Universo?
Ure?a-López, L. Arturo;
Revista mexicana de física , 2007,
Abstract: current cosmological observations begin to guide theoretical speculation about the origin, evolution and composition of the universe. this is a small review of how observations can be used to validate and to make changes to the cosmological models that have been built up to now.
Qué nos dicen las observaciones cosmológicas sobre el Universo?
L. Arturo Ure?a-López
Revista mexicana de física , 2007,
Abstract: Las observaciones cosmol′ogicas actuales comienzan a guiar la especulaci′on te′orica alrededor del origen, evoluci′on y composici′on del universo. Se presenta aqu′ un peque no resumen de c′omo las observaciones pueden utilizarse para validar y sugerir cambios a los modelos cosmol′ogicos que se han construido hasta ahora.
Bose-Einstein condensation of relativistic Scalar Field Dark Matter
L. Arturo Ure?a-López
Physics , 2008, DOI: 10.1088/1475-7516/2009/01/014
Abstract: Standard thermodynamical results of ideal Bose gases are used to study the possible formation of a cosmological Bose-Einstein condensate in Scalar Field Dark Matter models; the main hypothesis is that the boson particles were in thermal equilibrium in the early Universe. It is then shown that the only relevant case needs the presence of both particles and anti-particles, and that it corresponds to models in which the bosonic particle is very light. Contrary to common wisdom, the condensate should be a relativistic phenomenon. Some cosmological implications are discussed in turn.
Growth Hormone Improves Growth Retardation Induced by Rapamycin without Blocking Its Antiproliferative and Antiangiogenic Effects on Rat Growth Plate
óscar álvarez-García, Enrique García-López, Vanessa Loredo, Helena Gil-Pe?a, Natalia Mejía-Gaviria, Julián Rodríguez-Suárez, Flor á. Ordó?ez, Fernando Santos
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034788
Abstract: Rapamycin, an immunosuppressant agent used in renal transplantation with antitumoral properties, has been reported to impair longitudinal growth in young individuals. As growth hormone (GH) can be used to treat growth retardation in transplanted children, we aimed this study to find out the effect of GH therapy in a model of young rat with growth retardation induced by rapamycin administration. Three groups of 4-week-old rats treated with vehicle (C), daily injections of rapamycin alone (RAPA) or in combination with GH (RGH) at pharmacological doses for 1 week were compared. GH treatment caused a 20% increase in both growth velocity and body length in RGH animals when compared with RAPA group. GH treatment did not increase circulating levels of insulin-like growth factor I, a systemic mediator of GH actions. Instead, GH promoted the maturation and hypertrophy of growth plate chondrocytes, an effect likely related to AKT and ERK1/2 mediated inactivation of GSK3β, increase of glycogen deposits and stabilization of β-catenin. Interestingly, GH did not interfere with the antiproliferative and antiangiogenic activities of rapamycin in the growth plate and did not cause changes in chondrocyte autophagy markers. In summary, these findings indicate that GH administration improves longitudinal growth in rapamycin-treated rats by specifically acting on the process of growth plate chondrocyte hypertrophy but not by counteracting the effects of rapamycin on proliferation and angiogenesis.
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