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Search Results: 1 - 10 of 334278 matches for " Hélène Tronchère "
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Genetic Interaction between MTMR2 and FIG4 Phospholipid Phosphatases Involved in Charcot-Marie-Tooth Neuropathies
Ilaria Vaccari,Giorgia Dina,Hélène Tronchère,Emily Kaufman,Ga?tan Chicanne,Federica Cerri,Lawrence Wrabetz,Bernard Payrastre,Angelo Quattrini,Lois S. Weisman,Miriam H. Meisler,Alessandra Bolino
PLOS Genetics , 2011, DOI: 10.1371/journal.pgen.1002319
Abstract: We previously reported that autosomal recessive demyelinating Charcot-Marie-Tooth (CMT) type 4B1 neuropathy with myelin outfoldings is caused by loss of MTMR2 (Myotubularin-related 2) in humans, and we created a faithful mouse model of the disease. MTMR2 dephosphorylates both PtdIns3P and PtdIns(3,5)P2, thereby regulating membrane trafficking. However, the function of MTMR2 and the role of the MTMR2 phospholipid phosphatase activity in vivo in the nerve still remain to be assessed. Mutations in FIG4 are associated with CMT4J neuropathy characterized by both axonal and myelin damage in peripheral nerve. Loss of Fig4 function in the plt (pale tremor) mouse produces spongiform degeneration of the brain and peripheral neuropathy. Since FIG4 has a role in generation of PtdIns(3,5)P2 and MTMR2 catalyzes its dephosphorylation, these two phosphatases might be expected to have opposite effects in the control of PtdIns(3,5)P2 homeostasis and their mutations might have compensatory effects in vivo. To explore the role of the MTMR2 phospholipid phosphatase activity in vivo, we generated and characterized the Mtmr2/Fig4 double null mutant mice. Here we provide strong evidence that Mtmr2 and Fig4 functionally interact in both Schwann cells and neurons, and we reveal for the first time a role of Mtmr2 in neurons in vivo. Our results also suggest that imbalance of PtdIns(3,5)P2 is at the basis of altered longitudinal myelin growth and of myelin outfolding formation. Reduction of Fig4 by null heterozygosity and downregulation of PIKfyve both rescue Mtmr2-null myelin outfoldings in vivo and in vitro.
Phosphatase-Dead Myotubularin Ameliorates X-Linked Centronuclear Myopathy Phenotypes in Mice
Leonela Amoasii equal contributor,Dimitri L. Bertazzi equal contributor,Hélène Tronchère,Karim Hnia,Ga?tan Chicanne,Bruno Rinaldi,Belinda S. Cowling,Arnaud Ferry,Bruno Klaholz,Bernard Payrastre ? ,Jocelyn Laporte ? ,Sylvie Friant ?
PLOS Genetics , 2012, DOI: 10.1371/journal.pgen.1002965
Abstract: Myotubularin MTM1 is a phosphoinositide (PPIn) 3-phosphatase mutated in X-linked centronuclear myopathy (XLCNM; myotubular myopathy). We investigated the involvement of MTM1 enzymatic activity on XLCNM phenotypes. Exogenous expression of human MTM1 in yeast resulted in vacuolar enlargement, as a consequence of its phosphatase activity. Expression of mutants from patients with different clinical progression and determination of PtdIns3P and PtdIns5P cellular levels confirmed the link between vacuolar morphology and MTM1 phosphatase activity, and showed that some disease mutants retain phosphatase activity. Viral gene transfer of phosphatase-dead myotubularin mutants (MTM1C375S and MTM1S376N) significantly improved most histological signs of XLCNM displayed by a Mtm1-null mouse, at similar levels as wild-type MTM1. Moreover, the MTM1C375S mutant improved muscle performance and restored the localization of nuclei, triad alignment, and the desmin intermediate filament network, while it did not normalize PtdIns3P levels, supporting phosphatase-independent roles of MTM1 in maintaining normal muscle performance and organelle positioning in skeletal muscle. Among the different XLCNM signs investigated, we identified only triad shape and fiber size distribution as being partially dependent on MTM1 phosphatase activity. In conclusion, this work uncovers MTM1 roles in the structural organization of muscle fibers that are independent of its enzymatic activity. This underlines that removal of enzymes should be used with care to conclude on the physiological importance of their activity.
O caso de uma comunidade avaliativa emergente: re-apropria??o pelos pares-multiplicadores da aprecia??o de suas próprias a??es preventivas contra DST/HIV/AIDS, Amazonas, Brasil
Laperrière, Hélène;
Interface - Comunica??o, Saúde, Educa??o , 2008, DOI: 10.1590/S1414-32832008000300006
Abstract: this paper reports on the systematization of an experience of evaluative and participative investigation relating to a dst/hiv/aids prevention program that was implemented in the red-light zones of a remote municipality in the state of amazonas. this perspective of qualitative evaluation emphasized issues that were not considered at the time of initially implementing this project among sex workers, relating to the dimensions of the constructions of collective meaning that were directly linked to the implementation of peer-based education. we have focused on this experience of community investigation with peer multipliers for the project and the reappropriation of the evaluation of their preventive and social actions. the results and discussion are presented as a double prism: 1 the sequence of "emerging" thematic questioning over the course of the participative action/investigation in the field; and 2 the pragmatic practices of the evaluative investigation within the sociocultural and sociopolitical context.
Evaluation of STD/HIV/AIDS peer-education and danger: a local perspective
Ciência & Saúde Coletiva , 2008, DOI: 10.1590/S1413-81232008000600016
Abstract: an evaluation of peer-education projects with sex workers, men who have sex with men and marginalized adolescents, was introduced in a remote region of brazil. the context of varied limits of predictability made it difficult to conduct inquiry. to go beyond available epidemiological surveys and questionnaires on sexual behavior, a self-evaluation aimed at increasing pragmatic knowledge about prevention in a challenging socio-political context. during five-months, a participatory-action research explored participant observation; individual and collective exchanges with users, peer-educators, coordinators, administrators, politicians and regional health professionals. collective understanding of peer-education in prostitution zones underlines the reality of unforeseen social repercussions and confluence/divergence of multiple actors' perspectives. it identifies meaningful dimensions at a community-level, such as the collective history and dangerous working conditions. nurses face complex struggles and negotiations over multiple actors in their practice. this study suggests that nurses have a role to play in the conceptualization of participatory evaluation. it also underlines the threats to their physical and social safety, which they might share with peer-educators.
Discovering popular education in professional community health care practices
Laperrière, Hélène;
Texto & Contexto - Enfermagem , 2007, DOI: 10.1590/S0104-07072007000200012
Abstract: exploring the relationship between popular education and health care as regards to public and community health nursing, a case is made that popular education is more than a pedagogical approach to teaching. following an inductive strategy, experiential vignettes of the author?s practices and commitments in impoverished brazilian populations are systematized to identify contributions of popular education in health care. experiential learning leads to a preferential option for the poor, a reduction of power inequalities between program agents and the people, the use of observe-judge-act methodology and a theory that is realistic in terms of possible changes of consciousness. popular education fosters both the emergence of tacit nursing care actions and includes those of voiceless populations living in poverty.
Community health nursing practices in contexts of poverty, uncertainty and unpredictability: a systematization of personal experiences
Laperrière, Hélène;
Revista Latino-Americana de Enfermagem , 2007, DOI: 10.1590/S0104-11692007000700002
Abstract: several years of professional nursing practices, while living in the poorest neighbourhoods in the outlying areas of brazil's amazon region, have led the author to develop a better understanding of marginalized populations. providing care to people with leprosy and sex workers in riverside communities has taken place in conditions of uncertainty, insecurity, unpredictability and institutional violence. the question raised is how we can develop community health nursing practices in this context. a systematization of personal experiences based on popular education is used and analyzed as a way of learning by obtaining scientific knowledge through critical analysis of field practices. ties of solidarity and belonging developed in informal, mutual-help action groups are promising avenues for research and the development of knowledge in health promotion, prevention and community care and a necessary contribution to national public health programmers.
La inclusión de la perspectiva de la sociedad civil popular en VIH/SIDA: sistematización de experiencias como enfermera en Brasil y Canadá
Laperrière, Hélène;
Saúde e Sociedade , 2011, DOI: 10.1590/S0104-12902011000300011
Abstract: nurses in public health have the mission to mobilize the community-based organizations for the implementation of national health programmes. the work engages a proximity that will allow them to seize the details of a concrete reality, but which only a certain critical distance will lead to place them in a context of global meaning. a systematization of experiences presents the community evaluative research as a methodological opportunity for health and nursing sciences. the perspective is particularly relevant for health professional practices, especially in contexts of unpredictability and of sociopolitical conflicts of verticality among multiple actors involved in a public health program. the field insertion in marginalized districts and the work with community groups favor the inclusion of the popular civil society perspective in evaluations of aids preventive actions and other public health programmes.
Evaluationsforschung in den Kontexten soziopolitischer und soziokultureller Bewegungen: Methodologische Herausforderungen und die Dringlichkeit von Public-Health-Ma nahmen in entlegenen Regionen Evaluative Research in the Contexts of Sociopolitical and Sociocultural Movements: Methodological Challenges and the Urgency of Public Health Actions in Remote Regions La investigación evaluativa de los co
Hélène Laperrière
Forum : Qualitative Social Research , 2006,
Abstract: Der vorliegende Beitrag behandelt ein qualitatives Evaluationsprojekt, das sich mit partizipativen Bildungsprozessen von Prostituierten in einer entlegenen brasilianischen Region befasste. Im Zentrum des Projektes standen die Bedeutung unvorhergesehner Faktoren auf die Evaluationsergebnisse, die Bedeutsamkeit kollektiver Lernformen sowie die aktive Teilhabe lokaler sozialer Akteure bei der Gesundheitspr vention. Für die Evaluation wurden teilnehmende Beobachtung sowie individuelle und Gruppeninterviews in einer ethnographischen Perspektive genutzt unter Hinzuziehung g ngiger lateinamerikanischer Konzepte von Volksbildung für die Rekonstruktion kultureller Sinngebung. Im Rahmen der Studie wurden zun chst unbeabsichtigte sekund re Einflussfaktoren erkennbar, z.B. die Entwicklung von wechselseitigen Hilfepraktiken und nderungen in individuellen und kollektiven Lebensverl ufen, sowie nderungen in den kollektiven Bedeutungszuschreibungen, die aus der (Wieder-) Gewinnung einer gemeinsamen kollektiven Geschichte erwuchsen. Dabei erlaubte die N he zu den lokalen Gegebenheiten die Teilhabe der Forschenden unmittelbar im sozialpolitischen und -kulturellen Programmkontext. Dies wiederum verbesserte die M glichkeit, ungeplante Faktoren mittels eines qualitativen und partizipativen Ansatzes zu identifizieren. URN: urn:nbn:de:0114-fqs060464 A qualitative evaluation research project concerning peer-education among sex workers was carried out in a remote region of Brazil. The project focused on the impact of unpredictable factors on evaluation results, the importance attached to collective forms of experiential learning and the active participation of local social actors in public health actions (i.e. prevention). The evaluation combined a community inquiry perspective (participant observation, individual and group interviews) with an ethnographic emphasis, using prevailing Latin American views on popular education in the search for cultural meanings. The study revealed the project's unanticipated "secondary impacts," such as the development of mutual help practices and changes in personal and collective life trajectories, and changes in collective meaning attributions derived from the recovery of a collective history. Working in close proximity to the setting in which the program was implemented permitted access to the socio-cultural and socio-political movements in force at the time of implementation. Proximity to the local settings enabled the researcher to insert herself into the program's socio-cultural and socio-political context. The research might better deal
Discovering popular education in professional community health care practices
Hélène Laperrière
Texto & Contexto - Enfermagem , 2007,
Abstract: La exploración de la relación de la educación popular y los servicios de salud en materia de enfermería en salud pública y comunitaria permite mostrar cómo la educación popular es algo más que un enfoque pedagógico en la ense anza. Siguiendo una estrategia inductiva, vi etas que reflejan las prácticas e implicaciones de la autora en poblaciones brasile as marginales son sistematizadas con el fin de identificar las contribuciones de la educación popular a la salud pública. El aprendizaje vivencial realizado orienta a una opción preferencial por los pobres, una reducción de las desigualdades de poder entre los agentes de los programa y la población, el uso de la metodología de Ver, Juzgar, Actuar, y, de una teoría realista respecto a los posibles cambios de conciencia de los que viven en la miseria.
Réflexion sur la pratique infirmière avancée en soins communautaires dans le contexte de vulnérabilité
Hélène Laperrière
L'Infirmière Clinicienne , 2006,
Abstract: Cet article présente une réflexion critique sur les enjeux de la pratique infirmière avancée (PIA) en soins communautaires, notamment auprès de populations vivant dans un contexte de vulnérabilité sociale. Le travail révise l’usage galvaudé des concepts de vulnérabilité, de participation, d’autonomie et d’empowerment dans les discours en soins communautaires. Il explore le potentiel des fonctions professionnelles: (1) être un médiateur entre les niveaux locaux, régionaux et nationaux (communauté - réseau de santé et université); (2) éduquer à voir et à agir ensemble dans la précarité et l’insécurité; (3) développer la PIA par la médiation théorique-pratique de la recherche dans l’action; (4) concrétiser l’éthique professionnelle en un compromis d’action sociale. L’auteur conclut avec l’affirmation que l’autonomie de l’infirmière peut se développer par une prise de conscience de ses choix personnels, de ses valeurs, de ses engagements, bref, de son pourquoi agir qui accompagne son comment agir en société.
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