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Search Results: 1 - 10 of 541699 matches for " Héctor M.; Silva Cabrera "
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Resultados preliminares del perfeccionamiento del DAVIH-AgP24 con el empleo del sistema de amplificación biotina-tiramina/estreptavidina-peroxidasa Preliminary results of DAVIH-AgP24 with the biotin-tiramyne/streptavidine-peroxidase amplification system
Anitza Fragas Quintero,Eva Ortiz Losada,Héctor M. Díaz Torres,Eladio Silva Cabrera
Revista Cubana de Medicina Tropical , 2009,
Abstract: OBJETIVO: se modificó el ELISA DAVIH-Ag P24 con la introducción de la disociación por calor de las muestras de plasma y el empleo de un sistema de amplificación biotina-tiramina/estreptavidina-peroxidasa, para incrementar su sensibilidad. MéTODOS: se determinó la repetibilidad interensayo en el DAVIH-Ag P24 amplificado. Se evaluaron 32 muestras de plasma de individuos infectados por el VIH-1 en 3 categorías clínicas (caso SIDA, asintomáticos y con infecciones oportunistas menores). RESULTADOS: en la determinación de la repetibilidad interensayo se obtuvo un coeficiente de variación entre 4 y 10,3 %. Con el DAVIH-Ag P24 amplificado se incrementó el nivel de detección de P 24 hasta 0,5 pg/mL. El DAVIH-Ag P24 amplificado alcanzó 66 % de sensibilidad, mientras que el DAVIH-Ag P24 obtuvo 31 %. CONCLUSIONES: este estudio preliminar permitió demostrar que la incorporación de las nuevas modificaciones al sistema DAVIH-Ag P24 amplificado logró aumentar los niveles de detección de P24 y ganar en sensibilidad. OBJECTIVE: ELISA DAVIH-Ag p24 was modified by introducing heat dissociation of plasma samples and a tyramine/streptavidine-peroxidase amplification system, with the objective of increasing sensitivity. METHODS: Between-assay repeatability was determined in amplified DAVIH-Ag p24. Thirty two plasma samples from HIV-1-infected individuals classified in three clinical categories (AIDS case, asymptomatic and minor opportunistic infections) were evaluated. RESULTS: the variation coefficient ranged 4-10.3 % in between-assay repeatability. With the amplified DAVIH-p24 Ag, the p24 antigen detection level increased to 0.5 pg/mL. Amplified DAVIH-p24 Ag reached 66 % sensitivity whereas standard DAVIH-p24 Ag sensitivity rate was 31 %. CONCLUSIONS: This preliminary study proved that the introduction of new modifications in amplified DAVIH-p24 Ag managed to increase the p24 antigen detection levels and to gain sensitivity.
Resultados preliminares del perfeccionamiento del DAVIH-AgP24 con el empleo del sistema de amplificación biotina-tiramina/estreptavidina-peroxidasa
Fragas Quintero,Anitza; Ortiz Losada,Eva; Díaz Torres,Héctor M.; Silva Cabrera,Eladio; Izquierdo Márquez,Maricela;
Revista Cubana de Medicina Tropical , 2009,
Abstract: objective: elisa davih-ag p24 was modified by introducing heat dissociation of plasma samples and a tyramine/streptavidine-peroxidase amplification system, with the objective of increasing sensitivity. methods: between-assay repeatability was determined in amplified davih-ag p24. thirty two plasma samples from hiv-1-infected individuals classified in three clinical categories (aids case, asymptomatic and minor opportunistic infections) were evaluated. results: the variation coefficient ranged 4-10.3 % in between-assay repeatability. with the amplified davih-p24 ag, the p24 antigen detection level increased to 0.5 pg/ml. amplified davih-p24 ag reached 66 % sensitivity whereas standard davih-p24 ag sensitivity rate was 31 %. conclusions: this preliminary study proved that the introduction of new modifications in amplified davih-p24 ag managed to increase the p24 antigen detection levels and to gain sensitivity.
áNGULOS DEL PLANP SAGITAL DE LA COLUMNA LUMBOSACRA EN UNA MUESTRA DE ADOLESCENTES DE LA CIUDAD DE TEMUCO, CHILE
Silva M.,Héctor;
Revista chilena de anatomía , 2001, DOI: 10.4067/S0716-98682001000300007
Abstract: the spine problems are high prevalence in the world and cause physical disability. this public health problem affects people when adult. the spine problems are multifactor and one of them is the growing lumbar lordosis, lumbosacral angles, and lordosis depth. the main goal in the present work was to know these variables in the adolescent, because there is little information about the adolescent morphology. we analyze fifty x ray pictures of the adolescent spine, between 13 and 17 years old, 24 male and 26 female of temuco. the total of the case, 76% of them had angles lumbosacral between 30 and 49o, this represent a normally in relation to the other authors. in relation to the lordosis angle, the mean was between 43o and 95o, the mode 50o, mean 62.84, median 62o, and standard deviation was 10.74o. the other variable, the lordosis depth, the mean was 17.08 mm, the maximum and minimum were 9 and 24 mm, the standard deviation was 3.7 and the mode was 15. it is very important to know the parameter of the adolescent column and alterations, to promote prevention program.
áNGULOS DEL PLANP SAGITAL DE LA COLUMNA LUMBOSACRA EN UNA MUESTRA DE ADOLESCENTES DE LA CIUDAD DE TEMUCO, CHILE SAGITAL ANGLES VIEW FROM LUMBOSACRAL SPINE A ADOLESCENTS SAMPLE FROM TEMUCO CITY, CHILE
Héctor Silva M.
Revista chilena de anatomía , 2001,
Abstract: Las disfunciones de la columna vertebral que producen incapacidad laboral, son de alta prevalencia en el mundo. Este problema de salud pública afecta a la mayoría de las personas principalmente en la edad media de la vida. Los problemas de columna son multifactoriales y uno de ellos es el aumento de la lordosis lumbar, por aumentos del ángulo lumbosacro, ángulo lordótico y profundidad de la lordosis. El propósito del estudio fue conocer como se comportan nuestros adolescentes con respecto a las variables se aladas. Para ello se analizaron 50 radiografías panorámicas de columna vertebral, de escolares entre 13 y 17 a os de ambos sexos, 24 hombres y 26 mujeres, de la ciudad de Temuco, Chile. Del total de los casos observados, el 76% de los sujetos tenían ángulo lumbosacro que variaba entre 30o a 49o, lo que representa una normalidad con respecto a lo encontrado por otros autores. En cuanto al ángulo de la lordosis éste varió desde 43o a 95o, siendo la moda 50o, promedio 62,84o y la mediana de 62o. En cuanto a los valores de dispersión, la desviación estándar fue de 10,74. y el promedio de la profundidad de la lordosis fue de 17,08 mm; los valores máximo y mínimo fueron de 9 y 24 mm, respectivamente, la desviación estándar fue de 3,7 y la moda 15. Es importante conocer los diversos parámetros de la columna de los adolescentes y sus posibles alteraciones, para iniciar programas masivos de prevención The spine problems are high prevalence in the world and cause physical disability. This public Health problem affects people when adult. The spine problems are multifactor and one of them is the growing lumbar lordosis, lumbosacral angles, and lordosis depth. The main goal in the present work was to know these variables in the adolescent, because there is little information about the adolescent morphology. We analyze fifty x ray pictures of the adolescent spine, between 13 and 17 years old, 24 male and 26 female of Temuco. The total of the case, 76% of them had angles lumbosacral between 30 and 49o, this represent a normally in relation to the other authors. In relation to the lordosis angle, the mean was between 43o and 95o, the mode 50o, mean 62.84, median 62o, and standard deviation was 10.74o. The other variable, the lordosis depth, the mean was 17.08 mm, the maximum and minimum were 9 and 24 mm, the standard deviation was 3.7 and the mode was 15. It is very important to know the parameter of the adolescent column and alterations, to promote prevention program.
Marcadores de progresión serológicos y celulares en pacientes cubanos infectados por VIH-1
Díaz Torres,Héctor Manuel; Ribas Antúnez,María de los ángeles; Lubián Caballero,Ana Luisa; Pérez Toledo,Liliana; Izquierdo Márquez,Maricela; Silva Cabrera,Eladio;
Revista Cubana de Medicina , 2001,
Abstract: the behavior of serologic and cell markers for disease progression in a group of cuban serologically-confirmed hiv-infected patients was studied. firstly, 228 subjects at different clinical stages were randomly selected. the level of antibodies to hiv-1 24 kd protein was determined in every case which were followed-up for 26.5 months to observe their clinical progression. at the beginning of the follow-up period, it was confirmed that 65 % of patients with minor opportunistic infections and 98 % of aids patients had low anti p24 antibody titers and that the clinical progression rate was significantly higher in the group of patients that entered the study with low titers or lack of detectable anti p24 antibodies. at the end of the follow-up period, a cross sectional study was performed on 100 of the 228 patients, which included determination of anti p24 antibody titers, p24 antigenemia detection and lymphocyte sub-population count through flow cytometry. there was a correlation among serologic tests, cd4+ cell counts and clinical staging. the reduction of anti p24 antibodies titers was seen as an early marker. the usefulness of the combined use of these markers in the clinical follow-up of those patients was pointed out
Dise?o y evaluación del sistema DAVIH VIH-2
Martín Alfonso,Dayamí; Silva Cabrera,Eladio; Pérez Guevara,María T.; Díaz Herrera,Dervel F.; Romero Martínez,Kenia; Díaz Torres,Héctor M.; Lubián Caballero,Ana L.; Ruiz Gutiérrez,Nancy; Ortiz Losada,Eva;
Revista Cubana de Medicina Tropical , 2007,
Abstract: the results of the design and evaluation of davih vih-2 diagnosing system, an indirect elisa for screening of hiv-2 antibodies, which uses a hiv-2 glycoprotein gp36 synthetic peptide in its solid phase, were exposed. in the system evaluation using who reference panels, 100 % sensitivity, 99,81 % specificity, 99,81% efficacy and very good concordance level (kappa = 0.978) were attained. serum samples of 959 individuals with undetermined or negative results to the hiv-1 antibodies confirmation (davih blot) were evaluated by the davih vih-2 system. twenty four samples were reactive, six of which had confirmed hiv-2 antibodies. these results allowed recommending the introduction of this diagnostic kit in the hiv infection diagnosing algorithm in cuba.
Análisis Comparativo de la Composición Corporal de árbitros de Fútbol de Brasil y Uruguay
da Silva,Alberto Inácio; de los Santos,Héctor; Cabrera,Carlos;
International Journal of Morphology , 2012, DOI: 10.4067/S0717-95022012000300019
Abstract: the aim of this study was to comparative analysis of morphological profile of elite soccer referees from brazil and uruguay.the sample consisted of 27 referees from brazil, with a mean age of 37.6 ± 4.2 years, weight 79.1 ± 7.9 kg and height 179.1 ± 4.8 cm; and 14 referees from uruguaymean age 37.8 ± 3 years, weight 74.3 ± 7.4 kg and height 175 ± 6.8 cm.the variables of body weight, height, skinfolds, body girths and bone widths were measured in order to estimate body composition and somatotype of the referees.to determine the body composition we used seven skinfolds, four circumferences and seven widths.with respect to the data of body composition, when comparing the data with the brazilian and uruguay, there was a significant statistical difference between body weight, bmi and body fat percentage (p> 0.05).although the somatotype of both groups are classified as endomorph-mesomorph (somatotype average 3.7 to 4.0 -2.0), the brazilian officials showed a body fat percentage higher, which might constitutea physical performance limiting factor during the match and could be monitored through a program of good eating habits and physical fitness.
Análisis Comparativo de la Composición Corporal de árbitros de Fútbol de Brasil y Uruguay Comparative Analysis of Body Composition of Football (Soccer) Referees from Brazil and Uruguay
Alberto Inácio da Silva,Héctor de los Santos,Carlos Cabrera
International Journal of Morphology , 2012,
Abstract: El objetivo de este estudio fue hacer un análisis comparativo del perfil morfológico de árbitros de fútbol de elite de Brasil y Uruguay. La muestra fue compuesta por 27 árbitros del Brasil, con edad promedio de 37,6±4,2 a os, peso de 79,1±7,9 kg y estatura de 179,1±4,8 cm y 14 árbitros de Uruguay con edad promedio de 37,8 ± 3 a os, peso de 74,3 ± 7,4 kg y estatura de 175 ± 6,8 cm. Las variables de masa corporal, estatura, grosor de pliegues cutáneos, circunferencias corporales y diámetros óseos, fueron medidas con la finalidad de estimar la composición corporal y determinar el somatotipo de los árbitros. Para determinar la composición corporal se utilizaron: siete pliegues cutáneos, siete circunferencias corporales y cuatro diámetros óseos. Con respecto a los datos de composición corporal cuando se comparó los datos de los árbitros brasile os con los uruguayos se verificó una diferencia estadísticamente significativa entre el peso corporal, IMC y porcentaje de grasa corporal (p>0,05). Aunque el somatotipo de ambos grupos serán clasificados como endomorfo-mesomorfo (somatotipo promedio de 3,7- 4,0 -2,0), los árbitros brasile os presentaron una porcentaje de grasa corporal muy elevada lo que puede ser un factor limitante en la performance física durante el partido y que podría ser controlado por medio de programas de buenos hábitos alimenticios como de acondicionamiento físico. The aim of this study was to comparative analysis of morphological profile of elite soccer referees from Brazil and Uruguay.The sample consisted of 27 referees from Brazil, with a mean age of 37.6 ± 4.2 years, weight 79.1 ± 7.9 kg and height 179.1 ± 4.8 cm; and 14 referees from Uruguaymean age 37.8 ± 3 years, weight 74.3 ± 7.4 kg and height 175 ± 6.8 cm.The variables of body weight, height, skinfolds, body girths and bone widths were measured in order to estimate body composition and somatotype of the referees.To determine the body composition we used seven skinfolds, four circumferences and seven widths.With respect to the data of body composition, when comparing the data with the Brazilian and Uruguay, there was a significant statistical difference between body weight, BMI and body fat percentage (p> 0.05).Although the somatotype of both groups are classified as endomorph-mesomorph (somatotype average 3.7 to 4.0 -2.0), the Brazilian officials showed a body fat percentage higher, which might constitutea physical performance limiting factor during the match and could be monitored through a program of good eating habits and physical fitness.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Ana Luisa Lubián Caballero,Héctor Manuel Díaz Torres,Eladio Silva Cabrera,María Teresa Pérez Guevara
Revista Cubana de Medicina , 1998,
Abstract: Se estudiaron, en el período comprendido desde 1991 hasta 1996, 26 352 muestras de suero procedentes de diferentes grupos de riesgo y donantes de sangre para conocer la seroprevalencia de anticuerpos contra el virus linfotrópico de las células T humanas tipo I (HTLV-I) y como continuación de investigaciones realizadas entre 1989 y 1990. Se empleó el sistema de ELISA DAVIH-HTLV-I para el pesquisaje de anticuerpos y como prueba confirmatoria, el Western Blot DAVIH-Blot HTLV-I, ambos de Laboratorios DAVIH, Cuba. Se confirmó la presencia de anticuerpos anti HTLV-I en 10 personas y en la mayoría de ellas el estudio epidemiológico logró esclarecer la vía probable de contagio. El índice de seropositividad observado fue de 0,037 %, lo que en comparación con las tasas de seroprevalencia reportadas para el área del Caribe resulta bajo. In the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human T-lymphotropic virus type I (HTLV-I), and also as a continuation of researches carried out between 1989 and 1990. The ELISA DAVIH-HTLV-I system was used for the antibodies investigation, and as a certifying test, the Western Blot DAVIH-Blot HTLV-I was used too, both from the Cuban DAVIH Laboratories. The presence of anti HTLV-I antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. The seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the Caribbean area.
Detección de anticuerpos contra la proteína de 24 kd del VIH-1: Correlación clínico serológica
HéCTOR DíAZ TORRES,ELADIO SILVA CABRERA,ORFELINA RODRíGUEZ GARCíA,JORGE BáRCENAS MOSES
Revista Cubana de Medicina Tropical , 1996,
Abstract: Se estudió la presencia de anticuerpos contra la proteína de 24 kd del VIH mediante el empleo paralelo del Western Blot DAVIH BLOT y del DAVIH AC P24 en muestras de suero de 176 pacientes en diferentes estadíos de la infección por VIH-1. Los resultados se correlacionaron con la clasificación clínica del paciente al momento de la toma de muestra y con la evolución posterior durante 6 meses. El 57 % de los pacientes con infecciones oportunistas menores y el 96 % de los enfermos de SIDA presentaron títulos bajos de anticuerpos. Los fallecidos no mostraron reactividad o presentaron títulos muy bajos en muestras tomadas antes del fallecimiento. Se observaron diferentes titulaciones en grupos de sueros con reactividad aparentemente uniforme en el Western Blot. Los resultados indican una adecuada correlaciòn clínico serológica; por lo que el ELISA DAVIH AC P24 pudiera ser útil en el seguimiento clínico de personas infectadas por el VIH-1. The presence of antibodies against the HIV protein of 24 kd was studies by the parallel use of the DAVIH BLOT Western Blot and of the DAVIH AC P24 ELISA in serum samples from 176 patients at different HIV-1 infection stages. The results were correlated with the clinical classification of the patient at the moment of taking the sample and with the further evolution during 6 months. 57 % of the patients with opportunistic minor infections and 96 % of AIDS patients had low antibodies titres. Dead pattients showed no reactivity or presented very low titres in samples taken before dying. Different titrations were observed in serum groups with an apparently uniform reactivity in the Western Blot. The results show and adequate clinical and serological correlation. Therefore, the DAVIH AC P24 ELISA could be useful in the clinical follow-up of HIV-1 infected persons.
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