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Prevalence of G6PD deficiency in neonates referred to Semnan University
Nazari H
Medical Laboratory Journal , 2012, DOI: http://www.goums.ac.ir/mljgoums/index.php?slc_lang=en&sid=1
Abstract: Background and objectives: Glucose-6-phosphate dehydrogenase(G6PD) is an enzyme in the pentose phosphate pathway. G6PDdeficiency (an X-linked recessive hereditary disease) is an inheritedcondition affecting approximately 3% of the people globally. Thisdeficiency can cause hemolytic anemia and jaundice in neonates. Thegoal of this study is to detect the prevalence of G6PD deficiency inneonates referred to Semnan province screening labMaterial and Methods: This cross sectional study, from 2007 to2010, was conducted on the basis of country planned program and inline with neonatal screening tests. Blood samples were taken from theheels of 3-5 day neonates. Assessment of G6PD was done by rapidfluorescent spot test. The Prevalence of G6PD deficiency in boys andgirls was compared by chi square (p<0.05)test.Results: Of 9353 newborns referred to semnan province screeningLab., 4820(51.53%) are males and 4533(48.47%) females. Threehundred (3.2%) of them suffer from G6PD deficiency. Of these, 263(5.45%) are males and 37 (0.81%) females. The ratio of male tofemale is 7 to 1.Conclusion: The prevalence of G6PD deficiency is detected 3.2% inSemnan province and its frequency in boys is more than that of girls,which is expected.Key words: Glucose -6-phosphate dehydrogenase, Neonatalscreening, Fauvism
Assessment of Drought Tolerance in Barley Genotypes
L. Nazari,H. Pakniyat
Journal of Applied Sciences , 2010,
Abstract: In order to evaluate quantitative drought resistance criteria in some wild and cultivated barley, sixteen genotypes were tested under two different irrigation regimes (non-stressed and stressed). Plants were subjected to moisture stress at flowering period till maturity. Six drought tolerance indices, Stress Tolerance Index (STI), stress tolerance (TOL), Stress Susceptibility Index (SSI), Yield reduction ratio (Yr), Mean Productivity (MP) and Geometric Mean Productivity (GMP) were used. The indices were adjusted based on grain yield under stress (Ys) and non-stress (Yp) conditions. There were significant differences for all criteria among the genotypes. The significant and positive correlations of Yp with (MP, GMP and STI) and Ys with (MP, GMP and STI), as well as, significant negative correlation of SSI and TOL under stress environment, revealed that selection could be conducted for high values of MP, GMP and STI under both conditions and low values of SSI and TOL under stress condition. The correlation coefficients indicated that STI, MP and GMP are the best criteria for selection of high yielding genotypes both under stress and non-stress conditions. Results of calculated gain from indirect selection indicated that selection under moisture stress would be efficient in yield improvement compared to non-stress condition. Genotypes were significantly different for their yield under stress and non-stress conditions. Arivat (kavir), Aras, Goharjo and Afzal were the most desirable genotypes for both stress and non-stress environments.
Genetic Diversity of Wild and Cultivated Barley Genotypes Under Drought Stress Using RAPD Markers
L. Nazari,H. Pakniyat
Biotechnology , 2008,
Abstract: The genetic diversity among cultivated barley with different responses to drought stress was investigated. Initial screens involved growing 16 cultivars under drought conditions in greenhouse. These tests involved 5 tolerant and 5 sensitive types. The results of RAPD analysis indicated its suitability for determination of polymorphism among the samples. Among 30 primers used with RAPD-PCR technique, primers No. 3, 26 and 28 did not assist amplification of any definite bands and primers No. 24, 25 did not produce any scoreable polymorphic bands. In all the 25 selected primers amplified a total of 275 amplicons from 16 barley genotypes were detected, among them, 65 fragments (23.6%) were monomorphic and the rest (76.4%) were polymorphic between one or more genotypes. The cultivar genotypes were clustered according to their simple matching coefficient and complete-link methods. The least similarity was observed between wild genotype (Plot 21) and Valfajr. In general, Rihane with the average of 50.6% and Kavir with the mean of 60.8% had the least and the most similarity with other genotypes, respectively. Primer No. 27 amplified a 600 base pair fragment characteristic of tolerant cultivar lines and absent in susceptible genotypes. Therefore, it is likely that this DNA band be associated with drought tolerance. Clustering on the basis of 51% similarity ranked genotypes into 5 groups. The resulting dendrogram indicated that the cross of tolerant wild genotype (Plot 21) and susceptible Sina genotype which have low genetic similarity, also grouped in distinct cluster. This may be suggested as the most suitable cross to analyze QTLs (Quantitative Trait Loci) involved in drought tolerance.
Determination of Trace Amounts of Lead by Modified Graphite Furnace Atomic Absorption Spectrometry after Liquid Phase Microextraction with Pyrimidine-2-thiol  [PDF]
Saeid Nazari
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.27087
Abstract: The liquid phase microextraction (LPME) was combined with the modified Graphite furnace atomic absorption spectrometry (GF-AAS) for determination of lead in the water and solid samples. In a preconcentration step, lead was extracted from a 2 ml of its aqueous sample in the pH = 5 as lead-Pyrimidine-2-thiol cationic complex into a 4 µl drop of 1,2 dichloroethane and ammonium tetraphenylborate as counter ion immersed in the solution. In the drop, the lead-Pyrimidine-2-thiol ammonium tetraphenylborate ion associated complex was formed. After extraction, the microdrop was retracted and directly transferred into a graphite tube modified by [W.Pd.Mg] (c). Some effective parameters on extraction and complex formation, such as type and volume of organic solvent, pH, concentration of chelating agent and counter ion, extraction time, stirring rate and effect of salt were optimized. Under the optimum conditions, the enrichment factor and recovery were 525% and 94%, respectively. The calibration graph was linear in the range of 0.01 - 12 µg?L–1 with correlation coefficient of 0.9975 under the optimum conditions of the recommended procedure. The detection limit based on the 3Sb criterion was 0.0072 µg?L–1 and relative standard deviation (RSD) for ten replicate measurement of 0.1 µg?L–1 and 0.4 µg?L–1 lead was 4.5% and 3.8% respectively. The characteristic concentration was 0.0065 µg?L–1 equivalent to a characteristic mass of 26 fg. The results for determination of lead in reference materials, spiked tap water and seawater demonstrated the accuracy, recovery and applicability of the presented method.
EVALUATION OF AFLATOXINS CONTAMINATION IN BABY FOOD SUPPLEMENTS (MAMANA & GHONCHEH)
H Kalantari,GH Kalantari,Z Nazari Khorasgani
Jundishapur Journal of Natural Pharmaceutical Products , 2011,
Abstract: One of the most important sources of human nutrition is cereals such as rice, wheat, corn and barley. Baby food supplements mostly consist of cereals. If these products are improperly prepared, they may get contaminated with various aflatoxins such as AFB1, AFB2, AFG1 and AFG2. The purpose of this investigation was to determine the aflatoxins contamination of baby food supplements such as Mamana and Ghoncheh, which are widely used in Iranian markets. In this study 14 samples of Mamana and 15 samples of Ghoncheh were investigated for aflatoxin B1, B2, and G1. According to the CB method, extraction was carried out and then qualitatively and quantitatively by TLC-Scanner aflatoxins B1, B2 and G1 were identified and measured. The results of this study showed that 2 of 15 samples of Mamana and 2 of 14 samples of Ghoncheh were contaminated with aflatoxin B1 and B2. (< 2ppb).
"EVALUATION OF THE INCIDENCE AND OUTCOME OF FETAL HYDRONEPHROSIS PRENATALLY DIAGNOSED BY ULTRASOUND "
M. Pourissa,S. Refahi H. Nazari
Acta Medica Iranica , 2005,
Abstract: The outcome and proper management of fetal hydronephrosis have not been completely defined. The purpose of this study was to determine incidence and outcome of infants with a history of prenatal hydronephrosis diagnosed by ultrasound. In a three-year period from 2000 to 2003 we identified 211 cases (271 renal units) of fetal hydronephrosis in which renal pelvic anteroposterior diameter was more than normal diameter at various stages of pregnancy. Incidence of fetal hydronephrosis was 3.8 percent in the 55500 fetuses screened by ultrasound. A total of 187 fetuses (240 renal units) followed up underwent postnatal sonography and only in ten cases renal pelvic anteroposterior diameter was greater than 15 mm that required surgical correction . The most important advantage of knowing that a fetus has ultrasound findings of persistent hydronephrosis is ability to begin timely evaluation of the newborn to identify severity of hydronephrosis and prevent progressive deterioration of renal function.
Prevalence Of Depressive Disordrs In Tehran Resident Population (year 2000)
Kaviani H,Ahmadi Abhari A S,Nazari H,Honnozi K
Tehran University Medical Journal , 2002,
Abstract: Depression is a debilitating disease that every one is likely to experience over a short or long term period of his or her life."nMethods and Materials: This study aimed to examine the one - month prevalence of anxiety and depression in Tehranian resident population. 1070 men and women (age 20-65) were screened by Beck Depression Inventory (BDI). Then, those who scored above the cut - off point were psychiatrically interviewed. The interviewers were blind to the respondents' scores on BDI; 5% of the total sample were also added to the list of those to be interviewed. Interviewers were the third year psychiatric residents at Roozbeh hospital. Tehran, especially trained for this research's purpose."nResults: The results showed women (BDI- 12.16) are more depressed than men (BDI- 8.47). Furthermore, men (%16.7) were less likely to have depession disorders than women (% 30.50)."nConclusion: We will discuss discrepancies between (the present results and the results from previous research by others).
Epidemiological Pattern of Scabies and Its Social Determinant Factors in West of Iran  [PDF]
Mansour Nazari, Aziz Azizi
Health (Health) , 2014, DOI: 10.4236/health.2014.615231
Abstract: Objectives: Scabies has a different epidemiological distribution among different communities worldwide due to different social factors, management approaches and healthcare policies. The present study came to address outbreaks of scabies according to the social factors in west of Iran. Methods: In a cross-sectional study, using the census sampling method, all consecutive patients with the primary diagnosis of scabies based on clinical manifestations referred to healthcare center throughout the two great provinces of Hamadan and Kermanshah at western Iran between March 2006 and February 2010 were enrolled into the study. The baseline characteristics were collected from recorded files at the healthcare centers or by interviewing with the affected patients. Results: Among 3,625,966 subjects covered by the two studied provinces, 177 cases of scabies (170 cases in Kermanshah and 7 cases in Hamadan) were identified by medical staff at the healthcare centers according to clinical manifestations (85.3%) and laboratory microscopic assessments (14.7%). The highest and the lowest prevalence rates were specified to 2009 (58.2%) and 2008 (1.7%). Regarding gender distribution of disease, 53.1% of men and 46.9% of women suffered from scabies with no significant discrepancy. In respect to age distribution of disease, the highest rates of scabies were revealed in the ages ranged 17 to 30 years (31.6%), followed by younger than 17 years (30.5%). The prevalence of scabies was dependently associated with residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas. Conclusion: West region of Iran especially Kermanshah province faced with high prevalence and this high disease burden can be determined by some potential factors such as residency in rural areas, family history of scabies, lower educational level, household density, lower monthly income, low personal hygiene, the existence of livestock or rodents at home, seasonal conditions, and movement to contaminated areas.
Vaginitis in Intrauterine Contraceptive Device Users  [PDF]
Fatemeh Shobeiri, Mansour Nazari
Health (Health) , 2014, DOI: 10.4236/health.2014.611149
Abstract: Objectives: This study aimed to assess clinical, microbial changes in IUD users and other contraceptive methods in referent to urban health centers in Hamadan city, Iran. Methods: Detailed history and gynecological examination were conducted on women (IUD users, n = 100) or other contraceptive methods (controls, n = 160) in the health centers. Results: Frequencies of the different vaginal pathogens, high indices of infection by bacterial vaginosis (12.0%) candida albicance (7.0%) and low indices of trichomoniasis (5.0%) were found in present study, but none of them in both groups was significant. Menorrhagia was more frequent among women with IUD than that among women without IUD (P < 0.05). A trend of erosion cervix and dysmenorrhea being more frequent among women with IUD was also found (P < 0.05). Conclusions: IUD is the safety in general; however, an increase occurred in the frequency of vaginitis.
Prevalence of head and neck abnormalities among people with consanguineous parents
Razmpa E,Azimi C,Soltan Sanjarei M,Nazari H
Tehran University Medical Journal , 2008,
Abstract: "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 st1":*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: A high percentage of the patients at ENT or plastic surgery clinics have ENT abnormalities, either congenital or those developing later in life. Some are life threatening, while others cause disability and esthetic problems, with profound consequences for the affected child and the family. In addition, ENT abnormalities usually place stress on interpersonal relationships, causing social isolation, unhappiness and depression. The majority of these abnormalities is genetic and follows autosomal recessive pattern of inheritance. Geneticists believe that there is a higher frequency of autosomal recessive disorders among the offspring of consanguineous parents. The aim of this study was to find the frequency of ENT abnormalities among children born of consanguineous parents."n"n Methods: We studied 3503 files (pedigrees) of patients referred for genetic counseling at the Department of Genetics at Imam Khomeini Hospital, Tehran, Iran, from 1999 to 2000. We studied these cases according to degree of parental consanguinity, patterns of inheritance and gender using SPSS v.15."n"n Results: Of all 3503 files, 206 had ENT abnormalities, of which 157 had consangui-neous marriages. Among these 157 pedigrees, 496 cases had consanguineous parents. Out of the 496 cases, the four most frequent ENT abnormalities were: 115 cases (23.2%) of deafness, 53 cases (10.7%) of hearing loss, 12 cases (2.4%) of cleft lip and palate, and 5 cases (1%) of cleft palate."n"n Conclusion: This high frequency of ENT abnormalities indicates that consanguineous marriage should be discouraged by Iranian policy makers in order to reduce the burden of these disorders on society. Further research into the cultural problems that encourage consanguineous marriage and ways of resolving these problems is recommended. "nKeywords: Co
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