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Search Results: 1 - 10 of 683926 matches for " Gustavo A. S. Pinto "
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Selection of tannase-producing Aspergillus niger strains
Pinto, Gustavo A.S.;Leite, Selma G.F.;Terzi, Selma C.;Couri, Sonia;
Brazilian Journal of Microbiology , 2001, DOI: 10.1590/S1517-83822001000100006
Abstract: the aim of this work was to select strains of aspergillus niger for tannase production. growth of colonies in plates with tannic acid-containing medium indicated their ability to synthesize tannase. tannase activity was also measured in solid-state fermentation. a. niger 11t25a5 was the best tannase producer (67.5 u.g-1/72 hours of fermentation).
Selection of tannase-producing Aspergillus niger strains
Pinto Gustavo A.S.,Leite Selma G.F.,Terzi Selma C.,Couri Sonia
Brazilian Journal of Microbiology , 2001,
Abstract: The aim of this work was to select strains of Aspergillus niger for tannase production. Growth of colonies in plates with tannic acid-containing medium indicated their ability to synthesize tannase. Tannase activity was also measured in solid-state fermentation. A. niger 11T25A5 was the best tannase producer (67.5 U.g-1/72 hours of fermentation).
Canola Cake as a Potential Substrate for Proteolytic Enzymes Production by a Selected Strain of Aspergillus oryzae: Selection of Process Conditions and Product Characterization
Adriana C. Freitas,Ruann J. S. Castro,Maria A. Fontenele,Antonio S. Egito,Cristiane S. Farinas,Gustavo A. S. Pinto
ISRN Microbiology , 2013, DOI: 10.1155/2013/369082
Abstract: Oil cakes have excellent nutritional value and offer considerable potential for use in biotechnological processes that employ solid-state fermentation (SSF) for the production of high value products. This work evaluates the feasibility of using canola cake as a substrate for protease production by a selected strain of Aspergillus oryzae cultivated under SSF. The influences of the following process parameters were considered: initial substrate moisture content, incubation temperature, inoculum size, and pH of the buffer used for protease extraction and activity analysis. Maximum protease activity was obtained after cultivating Aspergillus oryzae CCBP 001 at 20°C, using an inoculum size of 107?spores/g in canola cake medium moistened with 40?mL of water to 100?g of cake. Cultivation and extraction under selected conditions increased protease activity 5.8-fold, compared to the initial conditions. Zymogram analysis of the enzymatic extract showed that the protease molecular weights varied between 31 and 200?kDa. The concentrated protease extract induced clotting of casein in 5?min. The results demonstrate the potential application of canola cake for protease production under SSF and contribute to the technological advances needed to increase the efficiency of processes designed to add value to agroindustrial wastes. 1. Introduction Proteases are one of the three largest groups of industrial enzymes and have a wide range of applications in the food, textile, and pharmaceutical industries [1]. Sources of proteases include plants, animals, and microorganisms. However, proteases from plant and animal sources are unable to meet global demand, as a result of which there is a need to develop efficient processes for microbial protease production. In fact, of the hundreds of enzymes currently used industrially, over half are derived from fungi and more than one-third from bacteria, with the remainder originating from animal (8%) and plant (4%) sources [2]. Microbial proteases account for approximately 40% of total worldwide sales of enzymes [3]. Processes that can be used for microbial protease production include submerged fermentation (SmF) and solid-state fermentation (SSF). The latter is particularly advantageous for enzyme production by filamentous fungi, since it simulates the natural habitat of these microorganisms [4, 5]. Another advantage of SSF is that agroindustrial wastes (oil cakes, bagasse, wheat bran, etc.) can be used as the solid substrate, acting as sources of both carbon and energy. However, certain operational limitations of SSF, such as
Structure Predictions of Two Bauhinia variegata Lectins Reveal Patterns of C-Terminal Properties in Single Chain Legume Lectins
Gustavo M. S. G. Moreira, Fabricio R. Concei??o, Alan J. A. McBride, Luciano da S. Pinto
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0081338
Abstract: Bauhinia variegata lectins (BVL-I and BVL-II) are single chain lectins isolated from the plant Bauhinia variegata. Single chain lectins undergo post-translational processing on its N-terminal and C-terminal regions, which determines their physiological targeting, carbohydrate binding activity and pattern of quaternary association. These two lectins are isoforms, BVL-I being highly glycosylated, and thus far, it has not been possible to determine their structures. The present study used prediction and validation algorithms to elucidate the likely structures of BVL-I and -II. The program Bhageerath-H was chosen from among three different structure prediction programs due to its better overall reliability. In order to predict the C-terminal region cleavage sites, other lectins known to have this modification were analysed and three rules were created: (1) the first amino acid of the excised peptide is small or hydrophobic; (2) the cleavage occurs after an acid, polar, or hydrophobic residue, but not after a basic one; and (3) the cleavage spot is located 5-8 residues after a conserved Leu amino acid. These rules predicted that BVL-I and –II would have fifteen C-terminal residues cleaved, and this was confirmed experimentally by Edman degradation sequencing of BVL-I. Furthermore, the C-terminal analyses predicted that only BVL-II underwent α-helical folding in this region, similar to that seen in SBA and DBL. Conversely, BVL-I and -II contained four conserved regions of a GS-I association, providing evidence of a previously undescribed X4+unusual oligomerisation between the truncated BVL-I and the intact BVL-II. This is the first report on the structural analysis of lectins from Bauhinia spp. and therefore is important for the characterisation C-terminal cleavage and patterns of quaternary association of single chain lectins.
Assistência multiprofissional em unidade de terapia intensiva ao paciente portador de síndrome de Prader-Willi: um enfoque odontológico
Setti, Juliana Santiago;Pinto, Sérgio Felix;Gaetti-Jardim, Ellen Cristina;Manrique, Gustavo Rodrigues;Mendon?a, José Carlos Garcia de;
Revista Brasileira de Terapia Intensiva , 2012, DOI: 10.1590/S0103-507X2012000100016
Abstract: prader-willi syndrome is a genetic neurobehavioral disease affecting children's development and resulting in obesity, reduced height, hypotonia, endocrine disorders and cognitive deficits, which may impair oral integrity. this study aims to report on a case involving a white male 15-year-old patient with prader-willi syndrome whose oral examination revealed bacterial plaque, gingivitis, poor occlusion, viscous salivation and multiple lip, jugal mucosa, inserted gum and tongue ulcerations. an excision biopsy revealed oral ulcerations typical of herpes, which were considered to be likely to correlate with herpes encephalitis. this result demonstrates that a large portion of the deleterious effects of prader-willi syndrome can be attenuated by appropriate diagnosis and early therapeutic intervention, highlighting the role of an integrated multidisciplinary team in the development of therapeutic protocols for prader-willi syndrome patients.
Mamíferos do Parque Florestal Estadual do Rio Doce, Minas Gerais, Brasil
Stallings, Jody R;Fonseca, Gustavo A. B. da;Pinto, Luiz Paulo de Souza;Aguiar, Ludmilla Moura de Souza;Sábato, Eduardo Lima;
Revista Brasileira de Zoologia , 1990, DOI: 10.1590/S0101-81751990000400022
Abstract: durante um período de 29 meses consecutivos foram realizados inventários de mamíferos em habitais de floresta nativa e exótica, além de área de campo, todos situados no parque florestal estadual do rio doce, localizado na regi?o ocidental da mata atlantica no estado de minas gerais. foram registradas para o parque um total de 60 espécies de mamíferos, distribuídas por 9 ordens, 24 famílias e 49 gêneros. de um esfor?o total de 64.300 armadilhas-noite, foram realizados 2.129 capturas de pequenos mamíferos n?o-voadores pertencentes a 20 espécies. foram também capturadas 14 espécies de quirópteros, representando 12 gêneros e 4 famílias. em 300 km de censos diurnos repetidos nas áreas de mata nativa foram registradas 12 espécies de mamíferos de médio e grande porte, além de 14 outras observadas através de meios diversos. estes dados refor?am a idéia que a mastofauna da mata atlantica é bastante diversa. o parque estadual florestal do rio doce deve ser considerado como uma das principais áreas para a conserva??o de fauna desta regi?o e sua prote??o deve ser priorizada.
Actividades enzimáticas en aislamientos bacterianos de tractos digestivos de larvas y del contenido de pupas de Automeris zugana y Rothschildia lebeau (Lepidoptera: Saturniidae)
Pinto-Tomás,Adrián; Uribe-Lorío,Lorena; Blanco,John; Fontecha,Gustavo; Rodríguez,César; Mora,Marielos; Janzen,Daniel; Chavarría,Felipe; Díaz,Joel; Sittenfeld,Ana;
Revista de Biología Tropical , 2007,
Abstract: enzymatic activities of bacteria isolated from the digestive tract of caterpillars and the pupal content of automeris zugana and rothschildia lebeau (lepidoptera: saturniidae). the enzymatic activities of bacteria isolated from the digestive tracts of caterpillars and the pupal contents of automeris zugana and rothschildia lebeau was studied. this digestive tract represents an extreme microenvironment due to its high ph and presence of antimicrobial substances secreted by the insect or derived from ingested plant tissue. at the same time, it contains large amounts of nutrient-rich food, for which microbes may compete among themselves and with the caterpillar. there is little information about the microbiota associated with tropical caterpillar guts, although bacteria from different genera have been isolated from gut and pupae samples. the study of the enzymatic activities generated by these organisms constitutes a starting point to understand their metabolic and physiological relationships with their hosts, and to find enzymes that have potential biotechnological applications. in this study we evaluated several enzymatic activities in two collections of bacteria isolated from caterpillar guts and pupae of the tropical lepidopteran species a. zugana and r. lebeau. bacteria grown under aerobic conditions were tested for an array of enzymes, including gelatinases, caseinases, lipases, esterases, cellulases, xylanases, amylases and chitinases. both collections displayed similar patterns of enzymatic activity. no isolate showed activity for all enzymatic tests, but as a whole, at least some bacteria in each collection were able to degrade each substrate tested. isolates with the same taxonomic identification obtained from caterpillar guts and pupae had almost the same enzymatic activities. in both collections, it was possible to group bacterial isolates according to their enzyme activity pattern. in addition to a heterogeneous ensemble of isolates exhibiting two or less
Dosse, Fran ois. La marcha de las ideas. Historia de los intelectuales, historia intelectual. Valencia: Universitat de València, 2007.
Gustavo A. Bedoya S.
Historia Crítica , 2012,
Fototerapia: aplica??es clínicas
Cestari, Tania F.;Pessato, Simone;Corrêa, Gustavo Pinto;
Anais Brasileiros de Dermatologia , 2007, DOI: 10.1590/S0365-05962007000100002
Abstract: phototherapy uses repeated controlled ultraviolet exposures to alter cutaneous biology, aiming to induce remission or control progression of skin diseases. this is an overview of the current practice of phototherapy applying ultraviolet radiation alone, in combination with photosensitizers or other medications. the mechanisms of action, the most accepted indications, regimens of prescription and side effects will also be discussed.
Publicación y gestión electrónica de manuscritos en Revista Científica Ciencia Médica: un paso adelante
Zuan P. Copana-Olmos,Gustavo A. Baumann-Pinto
Revista Científica Ciencia Médica , 2010,
Abstract: La Revista Científica Ciencia Médica desde su creación hace 13 a os, se ha intentado aprovechar al máximo las herramientas disponibles para la mejora de la calidad y difusión de la revista. Así, fue que fuimos una de las primeras revistas estudiantiles en nuestro medio en gestionar manuscritos e interactuar con el autor mediante el correo electrónico. Este pasado mes, pusimos en marcha el portal web de la revista y el sistema de gestión electrónica on-line de manuscritos, disponible en www.rev-ccm.umss.edu.bo. Somos por lo tanto la revista estudiantil de nuestro entorno pionera en ofrecer un formato electrónico en su presentación, y un sistema de gestión electrónica de manuscritos desde la web, este hecho es una de las mayores transformaciones que ha tenido la Revista Científica Ciencia Médica, desde su creación.
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