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Search Results: 1 - 10 of 78059 matches for " Guimar?es José Domingos "
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POSTPARTUM BOVINE ENDOMETRITIS TREATMENT BY INTRAUTERINE AND INTRAMUSCULAR ADMINISTRATION OF OXITETRACYCLINE TRATAMENTO DE ENDOMETRITE BOVINA PóS PUERPERAL PELAS VIAS INTRAMUSCULAR E INTRA-UTERINA
Anselmo Domingos Ferreira Santos,Eduardo Paulino da Costa,José Domingos Guimares,Hebert Rovay
Ciência Animal Brasileira , 2009,
Abstract: The aim of this study was to evaluate the efficiency of the postpartum bovine endometritis treatment by intrauterine and intramuscular administration of tetracycline. Forty six cross bred cows diagnosed by gynecological exam with postpartum endometritis were assigned randomly in two treatments: G1 (n=21) - animals were treated with one intramuscular (IM) dose of tetracycline (20 mg/Kg of body weight), while in G2 (n=25) animals were treated with one intrauterine (IU) dose of tetracycline (30 mg/Kg of body weight). No difference (p>0.05) was observed in the recovery rate between the two treatments (61.9 X 76.0%, G1 and G2, respectively). The interval from treatment until first estrous was 33.9±22.6 versus 14.8 ±10.9 days (P<0.05) and until first service was 54.7 ± 33.9 versus 27.2 ± 20.3 days (P<0.05;) for G1 and G2, respectively. No difference (p>0.05) was observed in the number of services per conception between G1 (1.54) and G2 (1.3). The treatment cost was lower for the G1 (U$ 3.51 versus U$ 5.00). Although not to have had differences in the clinical recovery rate, the use of the oxitetracycline managed for way IU in the postpuerperal treatment of endometrites in cows revealed more efficient in the reduction of the interval between the treatment and first heat and first insemination, beyond presenting lower cost that the treatment with oxitetracycline managed for way IM KEY WORDS: Bovine, postpartum endometritis, intrauterine, intramuscular, oxitetracicline. Este trabalho teve por objetivo comparar a eficiência do uso da oxitetracilina, administrada pelas vias intramuscular (IM) e intrauterina (IU), no tratamento de endometrites em vacas no período pós-puerperal. Foram utilizados 46 animais mesti os que apresentaram quadro clínico de endometrite, distribuídos aleatoriamente em dois tratamentos: G1 (n=21) – tratado com uma dose de 30 mg/Kg PV de oxitetraciclina por via IM, e G2 (n=25) – uma infus o de 20 mg/Kg PV de oxitetraciclina por via IU. N o houve diferen a (p>0,05) na taxa de recupera o dos animais, 61,9% e 76,0% para G1 e G2, respectivamente. O intervalo entre o tratamento e o primeiro estro (P<0,05) foi de 33,9 ± 22,6 versus 14,8 ± 10,9 dias, e do tratamento à primeira insemina o artificial (P<0,05) foi de 54,7 ± 33,9 versus 27,2 ± 20,3 dias para G1 e G2, respectivamente. N o houve diferen a entre o número de servi os por concep o entre G1 (1,54) e G2 (1,30). O tratamento pela via IU apresentou menor custo, quando comparado ao IM (P<0,05; R$ 6,54 e R$ 9,3, respectivamente). Apesar de n o ter havido diferen as na taxa de recupera o clínic
Semen quality and concentration of soluble proteins in the seminal plasma of Alpine bucks Semen quality and concentration of soluble proteins in the seminal plasma of Alpine bucks
Leonardo Franco Martins,Maria Cristina Baracat-Pereira,José Domingos Guimares,Simone Eliza Facione Guimares
Revista Brasileira de Saúde e Produ??o Animal , 2010,
Abstract: It was aimed to study the in vitro seminal quality analyzed by complementary tests and to compare them with physical, morphological and biochemical aspects of male goat semen of the Alpine breed. This experiment took place at the Federal University of Vi osa, situated at 20o45’ S latitude and 42o51’ W longitude, Southwest of Brazil. It was done during the summer months of January and February, and three adult male goats of the Alpine breed were used in intensive conditions. The semen was collected by artificial vagina method. In all semen samples (45 ejaculates), after the physical and morphological analysis, the hiposmotic test was done. In 24 ejaculates, it were done thermo-resistance test, and in 21 ejaculates it were determined the concentration of total soluble proteins in seminal plasma. The male goats presented difference in the semen physical and morphological aspects, in the hiposmotic test and thermo-resistance test, but they did not presented difference in total soluble proteins concentration in seminal plasma. Results of the slow thermo-resistance test and hiposmotic test were positively correlated (r = 0.60). It was concluded, according to our results, that the concentration of total soluble proteins in seminal plasma can not be used as a parameter to predict the seminal quality of Alpine bucks. It was aimed to study the in vitro seminal quality analyzed by complementary tests and to compare them with physical, morphological and biochemical aspects of male goat semen of the Alpine breed. This experiment took place at the Federal University of Vi osa, situated at 20o45’ S latitude and 42o51’ W longitude, Southwest of Brazil. It was done during the summer months of January and February, and three adult male goats of the Alpine breed were used in intensive conditions. The semen was collected by artificial vagina method. In all semen samples (45 ejaculates), after the physical and morphological analysis, the hiposmotic test was done. In 24 ejaculates, it were done thermo-resistance test, and in 21 ejaculates it were determined the concentration of total soluble proteins in seminal plasma. The male goats presented difference in the semen physical and morphological aspects, in the hiposmotic test and thermo-resistance test, but they did not presented difference in total soluble proteins concentration in seminal plasma. Results of the slow thermo-resistance test and hiposmotic test were positively correlated (r = 0.60). It was concluded, according to our results, that the concentration of total soluble proteins in seminal plasma can not be used as
Differential expression of genes in follicular cells of swines
Miranda-Furtado, Cristiana Libardi;Silva, Priscila Vendramini;Guimares, Marta Fonseca Martins;Ser?o, Nicola Vergara Lopes;Guimares, José Domingos;Guimares, Simone Eliza Facioni;
Revista Brasileira de Zootecnia , 2010, DOI: 10.1590/S1516-35982010000500012
Abstract: the main purpose of the present study was to identify for candidate genes related to ovulation in swines. to do so, it was investigated in ovarian follicular cells through quantitative real-time pcr the differential expression of the following genes: steroidogenic acute regulator (star), gata-binding protein 4 (gata), prostaglandin f2α (pgf2α), progesterone receptor (p4r), follicle-stimulating hormone receptor (fshr), and cytochrome p450 aromatase (cyp19). these genes encode hormone receptors (fshr and p4r), hormone (pgf2α), steroidogenic proteins (star and cyp19) and transcription factor (gata). folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. there was difference in transcript abundance among low and high prolific sows for the star, gata, pgf2α, p4r and cyp19 genes. for the fshr gene, the fold change was not considered to be significantly different. because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
Calagem e aduba??o mineral da soja (Glycine max (L.) Merrill) variedade Santa Maria, em solo de várzea
Baumgartner, José Geraldo;Lopes, Eli Sidney;Demattê, Joel Domingos;Miyasaka, Shiro;Igue, Toshio;Guimares, Geraldo;
Bragantia , 1974, DOI: 10.1590/S0006-87051974000100001
Abstract: a field experiment was carried out on a soil rich in organic matter to study the influence of lime and fertilizers on the production of soybean dry matter and on the soil chemical properties. three rates of lime (2.3, 4.6 and 6.9 t/ha), combined with two rates of superphosphate and one rate of potassium chloride were employed. molybdenum and boron were also applied in some treatments. neutralization of al3+ was not completed 162 days after liming. a time dependent ph variation was observed in the absence and presence of liming. dry matter and total nitrogen were increased by liming, but no differences were observed among liming rates. in the presence of lime the response to phosphorus was observed at the higher rate but only when potassium was also applied and for the lower liming rate; in the absence of lime only the higher rate of p application was effective, irrespective of the presence of potassium. applications of k, mo, and b did not increase the production.
Aduba??o nitrogenada do capim-elefante cv. Mott: 2. valor nutritivo ao atingir 80 e 120 cm de altura
Ribeiro, Karina Guimares;Gomide, José Alberto;Paciullo, Domingos Sávio Campos;
Revista Brasileira de Zootecnia , 1999, DOI: 10.1590/S1516-35981999000600004
Abstract: this trial was carried out, from october 1993 until june 1995, to evaluate the chemical composition and dry matter digestibility of dwarf elephantgrass at plant heights of 80 and 120 cm under five nitrogen rates. the experimental design was completely randomized with a split split plot arrangement, with four replicates, where the clipping factors, and nitrogen doses (0, 25, 50, 100kg/ha?clipping, in the first year, and 0, 75 and 100 kg ha?clipping, in the second year, and plant heights (80 and 120 cm) and its effects on the grass performance to yield were studied. the crude protein content in the leaf blades ranged, in the first year, from 8.8 to 13.2, in plants that reached 80 cm, and from 9.7 to 11,3% in plants with 120 cm, and in average, from 9.6 to 12.6%, in the second year, in response to the doses of nitrogen. the neutral detergent fiber, that was of 71.7% in the first year, varied from 67.4 to 71.1%, in plants with 80 and 120 cm, respectively, in the second year. the observed coefficients of ivdmd were 66.3 and 64.9% for plant heights of 80 and 120 cm, in the first year, and 69.0 and 61.5, in the second year, in the leaf blades of plants with 80 and 120 cm, respectively. the p, k and ca contents in the leaf blade inversely varied with the nitrogen doses, while the mg content did not present variation.
Eficiências Reprodutiva e Produtiva em Vacas das Ra as Gir, Holandês e Cruzadas Holandês x Zebu
Guimares José Domingos,Alves Nadja Gomes,Costa Eduardo Paulino da,Silva Márcio Ribeiro
Revista Brasileira de Zootecnia , 2002,
Abstract: Para avaliar a eficiência reprodutiva e sua rela o com características produtivas e composi o genética de um rebanho, criado em condi es de clima tropical, foram utilizadas 113 vacas, distribuídas em sete grupamentos genéticos, como se segue: 1/2, 3/4, 7/8, 9/16 e 15/16 Holandês x Zebu, Holandês--PB PC e Gir. As varáveis estudadas foram: idade ao parto, esta o de pari o, intervalo do parto ao primeiro servi o, intervalo do primeiro servi o ao servi o fértil, número de servi os, período de servi o, intervalo de partos e eficiência reprodutiva. Com rela o a características produtivas, avaliou-se o período de lacta o e produ o de leite por ordem de lacta o e grupamentos genéticos do rebanho. Foi utilizado o programa estatístico SAEG, realizando-se as análises de regress es múltiplas, de variancia e de compara o de médias pelo teste de Tukey a 5% de probabilidade de erro. A eficiência reprodutiva foi influenciada por idade ao parto, período de lacta o, intervalo de partos e composi o genética das vacas, porém n o o foi pela produ o de leite e esta o do ano. Os índices produtivos n o foram influenciados pela esta o de pari o, mas sim pela composi o genética dos animais. Neste estudo, os índices reprodutivos mostraram-se satisfatórios, os animais zebuínos apresentaram os menores índices produtivos e os taurinos, a menor eficiência reprodutiva, provavelmente pela maior sensibilidade às condi es de manejo.
Criopreserva o de ovócitos de bovinos imaturos desnudados ou n o, utilizando o etilenoglicol pelo método da vitrifica o
Costa Eduardo Paulino da,Guimares José Domingos,Torres Ciro Alexandre Alves,Fagundes Letícia Martins
Revista Brasileira de Zootecnia , 2002,
Abstract: Objetivou-se avaliar os efeitos da vitrifica o em ovócitos de bovinos após o cultivo in vitro, utilizando o etilenoglicol como crioprotetor. Ovócitos obtidos de ovários de vacas abatidas em matadouro foram distribuídos aleatoriamente em três tratamentos. Tratamento 0 (testemunha): ovócitos n o-desnudados e n o-congelados. Tratamento 1: vitrifica o de ovócitos imaturos n o desnudados, desidratados previamente por cinco minutos em três solu es contendo 20, 20 e 40% de etilenoglicol, acrescidas de 0,3 mol L-1 de trehalose e 20% de PVP, em meio de Talp Hepes. Tratamento 2: vitrifica o de ovócitos imaturos desnudados, conforme o Tratamento 1. Após o descongelamento (imers o em banho-maria a 30oC por 20 segundos), os ovócitos foram reidratados gradativamente, mantendo-os por 6 minutos em cada uma das solu es a seguir, sucessivamente: meio Talp Hepes com 20% de etilenoglicol + 0,3 mol L-1 de trehalose + 10% de PVP e meio Talp Hepes sem etilenoglicol, trehalose e PVP, onde foram lavados três vezes. Posteriormente, os ovócitos foram cultivados a 38,5oC, com 95% de umidade e atmosfera de 5% de CO2 por 24 horas. Após o cultivo, os ovócitos foram fecundados e os embri es cultivados in vitro por sete dias. Foi encontrada uma taxa de matura o nuclear de 81 (68/84), 19 (7/36) e 0% (0/31), nos Tratamentos 0, 1 e 2, respectivamente. As taxas de clivagem e de desenvolvimento embrionário foram de 56,4 (102/181) e 54,9% (56/102), 1,7 (1/60) e 0,0% (1/60), 0,0 (0/71) e 0,0% (0/71), nos Tratamentos 0, 1 e 2, respectivamente. Esses resultados indicam que o procedimento de vitrifica o, segundo os protocolos utilizados, n o é indicado para a criopreserva o de ovócitos de bovinos.
Atividade Ovariana em Fêmeas Bovinas da Ra a Holandesa e Mesti as Holandês x Zebu, Durante Dois Ciclos Estrais Normais Consecutivos
Alves Nadja Gomes,Costa Eduardo Paulino da,Guimares José Domingos,Silva Márcio Ribeiro
Revista Brasileira de Zootecnia , 2002,
Abstract: Foram utilizadas 25 fêmeas, distribuídas de acordo com o grau de sangue Holandês x Zebu nos grupos: I = 1/2 Holandês x Zebu; II = 3/4 Holandês x Zebu; III= 15/16 Holandês x Zebu; IV= Holandês--PB PC; e V= 7/8 Holandês x Zebu. Após o segundo estro natural pós-parto, esses animais foram monitorados por exames ultra-sonográficos diários por dois ciclos estrais consecutivos, para verificar a atividade ovariana quanto à dinamica folicular e à atividade do corpo lúteo. O período interovulatório médio foi de 22,08±2,46 dias, sendo de 21,76±3,33 e 21,94±2,90 dias no primeiro e no segundo ciclo estral, respectivamente, n o havendo diferen a entre os grupos estudados. Observou-se o crescimento de uma (6%), duas (78%) e três (16%) ondas foliculares durante o ciclo estral, com a predominancia de duas ondas foliculares. O período interovulatório dos ciclos com três ondas mostrou-se mais longo (25,33±4,37 dias), em compara o com uma (16,0±3,6 dias) e duas (21,83±1,77 dias) ondas foliculares. A dura o das ondas foliculares para ciclos estrais com três ondas de crescimento folicular foi de 11,0±3,69; 7,17±1,60; e 8,5±1,52 dias na 1a, 2a e 3a ondas, respectivamente, e de 11,15±1,65 e 12,07±2,32 dias nos ciclos estrais com duas ondas foliculares. Os dias de maior concentra o plasmática de progesterona no 1o e 2o ciclos estrais foram, respectivamente, de 13,6±3,0 (7,29±4,3 ng/mL) e 11,8±3,4 (5,6±2,0 ng/mL) dias, n o se mostrando diferentes entre os grupamentos genéticos e ciclos estrais estudados. Pelas análises dos dados, pode-se concluir que os diferentes grupamentos genéticos apresentaram o mesmo comportamento na dinamica folicular e que as concentra es de progesterona, embora diferentes em números absolutos entre os grupamentos, mostraram-se indicativas de atividade luteal normal.
Perfil eletrolítico e peso corporal em suínos submetidos a dietas com diferentes teores de fósforo
Dantas, Waleska de Melo Ferreira;Ribeiro Filho, José Dantas;Guimares, José Domingos;Farias, Sheila Kreutzfeld;Guimares, Simone Eliza Facioni;Saraiva, Alysson;Oliveira, Tania Toledo de;
Pesquisa Agropecuária Brasileira , 2010, DOI: 10.1590/S0100-204X2010001000021
Abstract: the objective of this work was to evaluate the effect of diets containing different levels of phosphorus on blood electrolytic profile and on body weight gain in pigs. sixty hybrid pigs were used, all barrows, with initial body weight of 61.07 ± 0.86 kg and about 110 days old. the animals were distributed in a randomized block design, in 5 treatments, with 12 replicates each. the treatments consisted of diets containing 0.135, 0.200, 0.265, 0.330 and 0.395% available phosphorus. at the beginning and at the end of the experiment, body weight was determined and blood samples were collected, in the animals without fasting, to measure sodium, potassium, chloride, total magnesium, phosphorus and ionized calcium. a quadratic response in body weight was observed, according to treatments, and for serum phosphorus, total magnesium and ionized calcium in the treatments. weight gain was higher in animals fed with phosphorus supplementation in levels above 0.135%. the best result occurred with 0.330% p supplementation, however, an increase from 0.200% in the diet caused an elevation in phosphorus serum concentration.
Uso de própolis e ácido ascórbico na criopreserva??o do sêmen caprino
Castilho, Erick Fonseca de;Guimares, José Domingos;Martins, Leonardo Franco;Pinho, Rogério Oliveira;Guimares, Simone Eliza Facioni;Espeschit, Cláudio José Borela;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009001200007
Abstract: the objectives of this study were to verify whether propolis and ascorbic acid have an effect on plasmatic membrane integrity of goat spermatozoa and investigate the potential of these antioxidants in the use of goats spermatozoa cryopreservation extenders. five adult boars were used of the alpine (n = 2) and saanen (n = 3) breeds. after semen collection, the evaluation consisted of the physical and morphological exam, live and dead cells (supravital test) and hyposmotic swelling test. afterwards, the fresh semen was diluted with the bioxcell? extender (control); bioxcell? + 0.25% freeze dried propolis extract); bioxcell? + 0.5% freeze dried propolis extract); bioxcell? + 0.05% ascorbic acid) or bioxcell? + 0.25% ascorbic acid). after final dilutions, the sperm motility and vigor were assessed obtained in each extender, and then the semen was sealed, cooled and frozen. in fresh semen, the physical and morphological aspects and the results of the supravital test and hyposmotic swelling test did not differ between animals or breeds. the general means of the sperm motility and vigor, supravital test and hyposmotic swelling test obtained immediately after thawing and after three hours of heat resistence test were different, so that the extender with ascorbic acid and the control were similar and higher than the extenders containing propolis. the ascorbic acid maintained the structural integrity of the spermatozoa membrane during the cryopreservation process and its viability after the heat resistance test, and may be an alternative in extender composition for cryopreservation of goat semen; the propolis was not effective in maintaining sperm integrity and viability after thawing and was toxic to spermatozoa at concentrations of 0.25 and 0.5%.
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