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Search Results: 1 - 10 of 2763 matches for " Guido Mastrobuoni "
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Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii
Guido Mastrobuoni, Susann Irgang, Matthias Pietzke, Markus Wenzel, Heike E Assmus, Waltraud X Schulze, Stefan Kempa
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-215
Abstract: To study de novo protein synthesis an arginine auxotroph Chlamydomonas strain was cultivated in presence of stable isotope-labeled arginine for 24 hours. From the time course experiment in 3 salt concentrations we could identify more than 2500 proteins and their H/L ratio in at least one experimental condition; for 998 protiens at least 3 ratio counts were detected in the 24?h time point (0?mM NaCl). After fractionation we could identify 3115 proteins and for 1765 of them we determined their de novo synthesis rate. Consistently with previous findings we showed that RuBisCO is among the most prominent proteins in the cell; and similar abundance and turnover for the small and large RuBisCO subunit could be calculated. The D1 protein was identified among proteins with a high synthesis rates. A global median half-life of 45?h was calculated for Chlamydomonas proteins under the chosen conditions.To investigate the temporal co-regulation of the proteome and metabolome, we applied salt stress to Chlamydomonas and studied the time dependent regulation of protein expression and changes in the metabolome. The main metabolic response to salt stress was observed within the amino acid metabolism. In particular, proline was up-regulated manifold and according to that an increased carbon flow within the proline biosynthetic pathway could be measured. In parallel the analysis of abundance and de novo synthesis of the corresponding enzymes revealed that metabolic rearrangements precede adjustments of protein abundance.
Exploring Proteins in Anopheles gambiae Male and Female Antennae through MALDI Mass Spectrometry Profiling
Francesca R. Dani, Simona Francese, Guido Mastrobuoni, Antonio Felicioli, Beniamino Caputo, Frederic Simard, Giuseppe Pieraccini, Gloriano Moneti, Mario Coluzzi, Alessandra della Torre, Stefano Turillazzi
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002822
Abstract: MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and small proteins in biological tissues. In this work we applied them to the study of Anopheles gambiae antennae, with the aim of analysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by direct profiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained through profiling had a richer ion population and higher signal to noise ratio. Male and female antennae showed distinct protein profiles. MALDI imaging experiments were also performed and differences were observed in the localization of some proteins. Two proteins were identified through high resolution measurement and top-down MS/MS experiments. A 8 kDa protein only present in the male antennae matched with an unannotated sequence of the An. gambiae genome, while the presence of odorant binding protein 9 (OBP-9) was confirmed through experiments of 2-DE, followed by MS and MS/MS analysis of digested spots. This work shows that MALDI MS profiling is a technique suitable for the analysis of proteins of small and medium MW in insect appendices, and allows obtaining data for several specimens which can be investigated for differences between groups. Proteins of interest can be identified through other complementary MS approaches.
Primi dati sulla chirotterofauna del Parco Nazionale del Circeo (Lazio)
Giovanni Mastrobuoni,S. Zerunian
Hystrix : the Italian Journal of Mammalogy , 2003, DOI: 10.4404/hystrix-14.0-4282
Abstract: Il Parco Nazionale del Circeo è situato sulla costa del Lazio meridionale, nella provincia di Latina, ed ha un?estensione di circa 8.400 ha. Il territorio è caratterizzato da una notevole complessità di ambienti rappresentati principalmente dalla foresta planiziale, da quattro laghi costieri (Fogliano, Monaci, Caprolace, Sabaudia) con gli acquitrini circostanti, dalla duna litoranea, dal Promontorio del Circeo (Picco di Circe 541 m s.l.m.) e dall?Isola di Zannone. Grazie alla sua elevata diversità ambientale ed all?ampia disponibilità di habitat acquatici importanti per il foraggiamento, il Parco è, potenzialmente, un?area di notevole interesse per i Chirotteri. Il livello di conoscenza scientifica di molti gruppi di Vertebrati terrestri del territorio dell?area protetta si presenta ancora molto scarno e lacunoso; in particolare i Chirotteri costituiscono molto probabilmente il gruppo in assoluto meno conosciuto. Dal febbraio 2002 gli autori hanno avviato una ricerca sulla composizione in specie e sulla distribuzione della chirotterofauna del Parco. Nell?ambito della ricerca sono state utilizzate tecniche di rilievo diretto, quali l?ispezione di cavità naturali ed artificiali, edifici, resti archeologici, ponti, con cattura e successivo rilascio di alcuni individui; con l?ausilio di un bat detector, nelle modalità ?divisione di frequenza? ed ?eterodina?, sono state effettuate perlustrazioni del territorio dell?area protetta per individuare quei siti caratterizzati da una notevole attività di volo e di foraggiamento e quindi predisporre, per la stagione di campo 2003, un protocollo di catture con mist nets. L?indagine ha permesso, fino ad oggi, di ottenere una prima serie di dati; è stata rilevata la presenza di: Rhinolophus ferrumequinum, Rhinolophus hipposideros, Myotis myotis, Myotis daubentonii, Pipistrellus kuhli, Tadarida teniotis. A queste vanno aggiunte tre specie rilevate fra il 1994 ed il 2001: Myotis emarginatus, Pipistrellus pygmaeus ed Hypsugo savii. La ricerca di rifugi invernali, condotta da ottobre 2002 a marzo 2003, non ha dato esito positivo. L?indagine relativa ai rifugi estivi, condotta nel 2002 ed attualmente in corso per il 2003, ha invece permesso di individuare una colonia mista di Rinolofidi e Vespertilionidi (settembre 2002) ed una colonia riproduttiva di Myotis daubentonii (giugno 2003); in alcuni siti sono stati trovati maschi isolati o in piccoli gruppi. I risultati finora ottenuti costituiscono sicuramente solo un primo quadro della chirotterofauna del Parco Nazionale del Circeo. Al fine di compilare una puntuale checklist d
A Proteomic Investigation of Soluble Olfactory Proteins in Anopheles gambiae
Guido Mastrobuoni, Huili Qiao, Immacolata Iovinella, Simona Sagona, Alberto Niccolini, Francesca Boscaro, Beniamino Caputo, Marta R. Orejuela, Alessandra della Torre, Stefan Kempa, Antonio Felicioli, Paolo Pelosi, Gloriano Moneti, Francesca Romana Dani
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075162
Abstract: Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector.
Does Gravitation Have an Influence on Electromagnetism?  [PDF]
Guido Zbiral
Journal of Modern Physics (JMP) , 2012, DOI: 10.4236/jmp.2012.329158
Abstract: For many years physicists have been engaged on research around the globe in fields such as the unification of gravita- tion and electromagnetism, and an explanation for dark matter and dark energy, etc., but so far to little avail. One is left with the impression that something might be fundamentally wrong with the premises underlying the doctrine of physics applicable today, which is preventing a solution of these problems from being found. As a possible cause, the author proposes that the gravitation of the photons is not so negligible that it can be completely ignored (although this assumption does not accord with the current state of physics). Departing therefore from the accepted doctrine, he assumes that gravitation might possess a hitherto unknown important influence on electromagnetism. This paper then examines the consequences of this assumption on physics. A precise analysis will lead to the insight that the gravitation of a photon is as dynamic as the photon itself, and therefore must be taken into account with all associated physical considerations. The hitherto accepted case of a static gravitation of photons, on the other hand, can be totally neglected, as it does not exist for photons. Of key importance is the statement that the gravitation of photons is produced by gravitational quanta, and thus appears in quantised form. It is therefore necessary to rethink the physics of photons. This leads to a number of other interesting insights, as will be borne out in the further course of this paper. In the event that the assumption of the influence of gravitation on electromagnetism turns out to be correct, then this would represent a major step in unravelling the still largely unknown nature of gravitation and its significance in the natural events of the microcosmos; furtheron it would be an important contribution regarding a “New Physics” and a “New Cosmology”.
The “Dynamic Gravitation of Photons: A Hitherto Unknown Physical Quantity”. New Aspects on the Physics of Photons  [PDF]
Guido Zbiral
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.55030
Abstract:

In order to explore the nature of photons, no doubts can be allowed to exist concerning the “physics of photons”. While static gravitation plays no role in the physics of photons, this paper will show that the previously unknown nonbaryonic dynamic gravitation of photons determines not only the external physical behaviour of photons but also, in particular, the hitherto unknown physical events occurring within the photons themselves. For this reason, the paper places particular emphasis on dynamic gravitation as a new hitherto unknown physical quantity. Moreover the new type of gravitation postulated here also provides a plausible explanation of the mysterious nonbaryonic dark matter. As no generally accepted scientific explanation of the creation and essence of dark matter exists to date, it is to be anticipated that the nonbaryonic dynamic gravitation of photons is of general interest to physicists as well as cosmologists and may serve to initiate a general debate among them. Furthermore, this paper will also show that there exists a close mutual relationship between electrodynamics <> dynamic gravitation <> static gravitation <> electrostatics <> electrodynamics (refer to paragraph 4). Due to the fact that the insights into the relationship between photons and their dynamic gravitation have not been described by any other author to date, there exists only a few references that I can cite in support of my paper.

On the Cosmic Ray Sources  [PDF]
Guido Pizzella
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.55034
Abstract:

The cosmic ray energy spectrum is calculated assuming that cosmic rays are generated by astrophysical objects provided by magnetospheres with a dipole magnetic field. With simple geometric considerations, the energy spectrum E-2.5 is obtained, independently on the particle species.

The Dynamic Gravitation of Photons from the Perspective of Maxwell’s Wave Equations  [PDF]
Guido Zbiral
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.512111
Abstract:

Although the gravitational constant (G) does not explicitly occur in the Maxwell Wave Equations, this paper will show that G is indeed implicitly contained in them. The logical consequence hereby is that electromagnetic radiation is associated with dynamic gravitation and notas assumed in Einstein’s Special Theory of Relativitywith “static” gravitation, dynamic gravitation being at the time unknown. According to the Maxwell Wave Equations, gravitation experiences the same dynamic (speed of light c) as electromagnetic radiation and must therefore also be of a quantum nature. There must exist an equal number of gravitational quanta as there are photons. Since photons do not possess a baryonic rest mass but only a relativistic mass, this mass must be nonbaryonic in nature—precisely as their dynamic gravitation.

Ideas on an Alternative Cosmological World Model with Different Initial Conditions  [PDF]
Guido Zbiral
Journal of Modern Physics (JMP) , 2015, DOI: 10.4236/jmp.2015.63037
Abstract: The actual world model, the “Standard Model of Cosmology” (SMC), which dates back to the 1950s, no longer corresponds to the latest state of knowledge on the cosmos. By way of example, the assumption made in the SMC that the expansion of the cosmos is continually being reduced due to the effect of the gravitation exerted on all the matter in the universe is now contradicted by recent measurements. The reason for the expansion of cosmic space in accordance with Hubble’s Law is not physically explained by the SMC but merely stated as a fact. Another example is provided by the “dark phenomena”, which make up by far the greatest part of the energy of the cosmos, and exert a dominant influence on its behaviour. In spite of intensive research over the decades to provide answers to these as well as other open issues in cosmology, no satisfactory and plausible answers have hitherto been found. It is indeed time to propose an alternative cosmological world model to the SMC in the light of the latest insights on the universe.
Considerations on the Unification of Quantum Physics with the General Theory of Relativity  [PDF]
Guido Zbiral
Journal of Modern Physics (JMP) , 2016, DOI: 10.4236/jmp.2016.710105
Abstract: From a holistic perspective of a physical space of any given size1, it is invariably necessary to consider its energy content, since no physical means exists of making a physical space completely devoid of energy. Such a space would therefore only be a fictive “geometric space”—that can be intellectually conceived and treated according to the rules of the appropriate geometry—although not existing in reality in the cosmos. Cosmic space always contains energy in one form or another, limited by the space under consideration. Therefore, each space possesses an energy density—no matter how low, which never becomes zero. Because of the mass-energy equivalence relationship \"\", cosmic space also possesses a mass equivalent and is therefore “materialistic” in nature. If this is considered in association with Einstein’s space-time, what is obtained instead is an “energy-time”, i.e. an energy effect, which is based on Planck’s action quantum h. Under this condition, a close relationship would appear to exist between the General Theory of Relativity and Quantum Physics. Furthermore, it will be shown that the physical conditions of space are such that a natural quantisation of space and time exists, thus obviating the need for any artificial or arbitrary quantisation.
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