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Search Results: 1 - 10 of 36920 matches for " Guangqiang ZHAO "
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Expression of PAH-DNA Adducts in Lung Tissues of Xuanwei Female Lung Cancer Patients
Kaiyun YANG,Yunchao HUANG,Guangqiang ZHAO,Yujie LEI
Chinese Journal of Lung Cancer , 2010,
Abstract: Background and objective The coal-fired pollution in Xuanwei area has been considered to be local main reason for high incidence of female lung cancer. The aim of this study is to explore the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and to explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancer. Methods We totally collected each 20 cases of Xuanwei female lung cancer patients, Xuanwei male lung cancer patients, Non-Xuanwei female lung cancer patients and collect each 10 cases of Xuanwei, Non-Xuanwei female patients with benign lung lesions. The cancer tissues, adjacent cancer tissues and normal lung tissues were collected in lung cancer patients and only the normal tissues were collected in benign lung lesion patients. There were total 80 cases and 200 tissues. Immunofluorescence was used to detect the expression of PAHDNA adducts in each group. Image pro-plus 6.0 software was used to analyze the images and part quantified analysis. SPSS 13.0 statistical software was used to analyze the data. Results The positive expression of PAH-DNA adducts in lung cancer tissues, adjacent cancer tissues and normal lung tissues of Xuanwei female lung cancer patients were 90%, 80% and 65%. They were higher than the positive expression of PAH-DNA adducts in Xuanwei male lung cancer patients (35%, 30%, 30%) and Non-Xuanwei female lung cancer patients (20%, 15%, 10%)(P < 0.01). The expressions in lung tissues of Xuanwei female benign lung lesion patients (positive expression is 70%) were higher than it in Non-Xuanwei female benign lung lesion patients (positive expression is 10%). With the direction changing from cancer tissues, adjacent cancer tissues to normal lung tissues, the expressions of PAH-DNA adducts were decreased but had no statistical difference (P>0.05). Conclusion The expressions of PAHDNA adducts in lung tissues of Xuanwei female were higher than which in Xuanwei male and Non-Xuanwei female.
Subcellular Distribution and Genotoxicity of Silica Nanoparticles in Human Bronchial Epithelial Cells
Guangqiang ZHAO,Yunchao HUANG,Guangjian LI,Sen LI
Chinese Journal of Lung Cancer , 2013, DOI: 10.3779/j.issn.1009-3419.2013.03.01
Abstract: Background and objective Silicon nanoparticles are widely used in daily life. Therefore, they attract increased attention because of their potential biotoxicity to the lungs when inhaled. The aims of this study are to explore the organism distribution and genotoxicity of silica nanoparticles in human bronchial epithelial cells (BEAS-2B). Methods The biodistribution of silica with different particle sizes in human bronchial epithelial cells was observed by transmission electron microscopy (TEM). DNA damage was detected by single-cell gel electrophoresis (comet assay). Results TEM revealed that SiO2 nanoparticles with different sizes can be uptaken by cells and be localized in the cytoplasm and the nucleus. Compared with micro-silica, nano-silica in BEAS-2B cells can inflict more severe DNA damage (P<0.05). Conclusion The particle size of silica nanoparticles can be used to determine their distribution in biological cells. Compared with micro-silica, nano-silica has higher genotoxicity.
Therapeutic efficacy of Traditional Vein Chemotherapy and Bronchial Arterial Infusion Combining with CIKs on Ⅲ Stage Non-small Cell Lung Cancer
Guangqiang ZHAO,Yunchao HUANG,Lianhua YE,Lincan DUAN
Chinese Journal of Lung Cancer , 2009,
Abstract: Background and objective The therapeutic efficacy of late lung-cancer was very poor, and cytokine-induced killer cells (CIK) were paid more attention to treat non-small cell lung cancer (NSCLC). The aim of this study is to get insight into the role of bronchial arterial infusion bronchial arterial infusion (BAI) plus CIK about NSCLC by comparing therapeutic efficacy among BAI, traditional vein chemotherapy and BAI plus CIK, for late NSCLC. Methods A total of 120 patients were enrolled in this study, dividing randomly into three groups: bronchial arterial infusion (BAI), traditional vein chemotherapy and BAI plus CIK. Clinical effects and side effects were estimated after two period of therapy. Results The effective rate (CR+PR%) of combined group is higher than the traditional vein chemotherapy group (66.67%, n=39) and there are significant differences (χ2=4.721, P=0.03); The side effect of rate of BAI plus CIK group is significantly lower than the traditional vein chemotherapy group, and so did the non-bone marrow inhibition side effects (P < 0.05). The tumor progression rate (PD%) of bronchial arterial infusion (BAI) group is higher than combined group (χ2=4.287, P=0.038). There was no difference between the traditional vein chemotherapy group and combined group (χ2=0.082, P=0.775). Conclusion Bronchial Artery Infusion combined with cytokine-induced killer cells is an ideal, safety, effective comprehensive treatment method for late stage lung cancer.
Effects of selenium and reduced glutathione on the proliferation and apoptosis of XWLC-05 cell
Lan ZHOU,Yunchao HUANG,Yujie LEI,Guangqiang ZHAO
Chinese Journal of Lung Cancer , 2008,
Abstract: Background and objective Selenium is an essential micronutrient for mammals but toxic in large amounts. Most studies indicate that selenium has inhibitory effect on cancer. The aim of this study is to investigate the effects of selenium and reduced glutathione (GSH) combined application on the proliferation and apoptosis of human lung adenocarcinoma cell line XWLC-05. Methods XWLC-05 cells were respectively treated in vitro by four factors (sodium selenite, GSH, sodium selenite+GSH and blank control (RMPI-1640 +10% calf serum) in different concentrations for 24 h. Cell growth inhibition rates were determined by MTT assay, cytomorphology was observed under inverted phase contrast microscope and changes of cell cycle were detected by Flow Cytometry (FCM). Results Both selenium and GSH individual on the XWLC-05 cells were found to possess obvious growth inhibition effect on the XWLC-05 cells. Selenium and GSH combined application on the XWLC-05 cells had cooperative inhibition effect (P<0.01). The inhibition rate was increased in a dose-dependent relationship as selenium with concentrations between (0.5-4.0) μg/mL (P<0.01) whether it was selenium single factors or selenium and GSH combined effect. FCM results showed that some XWLC-05 cells were induced apoptosis and G1 phase cells were markedly increased and S, G2/M phase cells decreased in both selenium individual groups and selenium and GSH combined groups. Conclusion Selenium and GSH combined application on XWLC-05 cells can enhance directly the cell growth inhibitory effect compared with selenium and GSH individual. The mechanism seems to inhibit the synthesis of RNA and protein and prevent cells from entering S phase.
Research on Fast Track Surgery Application in Lung Cancer Surgery
Guangqiang ZHAO,Yunchao HUANG,Xiaobo CHEN,Lincan DUAN
Chinese Journal of Lung Cancer , 2010,
Abstract: Background and objective Fast track surgery (FTS) is a systematical method to accelerate the recovery of surgical patients by reducing the physical and mental trauma stress of them. The research is to investigate the feasibility of FTS application in lung cancer surgery. Methods A total of 80 cases of lung cancer patients with single leaf lobotomy resection were randomized into two groups. While the experimental group was treated with the conception of FTS, and the control group was treated with the traditional methods. The incident rate of post-operation pain degrees, telecasts, pleural effusion, the post-operation time stay in hospital time and the total cost during hospitalization in two groups were compared respectively. Results In FTS group: the VAS score of post-operation pain at 1 h, 6 h, 12 h, 24 h and 48 h all significantly decreased compared to the traditional therapy group. The incidence rate of telecast was 10.53%. The incidence rate of pleural effusion was 26.31%. The length of stay after operation was (4±1) d and the total cost was RMB 15 600±7 600. In the control group, the above values were 77.78%, 33.33%, 22.22%, (9±1) d, RMB 23 600±5 400, respectively. The post operation pain (VAS method) of FTS group was remarkablely below the control group. There has significant difference of the incident rate of telecasts, stay time in hospital and the total cast in two groups (P < 0.05). No significant difference was observed in the incident rate of pleural effusion. Conclusion The new methods of FTS can apparently accelerates recovery after lung cancer resection, reduces complications, shorten timestay in hospital and cut down the total cost.
Study on the Relationship between the Inhalable Fine Particulate Matter of Xuanwei Coal Combustion and Lung Cancer
Jiapeng YANG, Yu CAO, Yunchao HUANG, Guangjian LI, Lianhua YE, Guangqiang ZHAO, Yujie LEI, Xiaobo CHEN, Linwei TIAN
- , 2015, DOI: : 10.3779/j.issn.1009-3419.2015.07.03
Abstract: Background and objective The high incidence of lung cancer in Xuanwei, China, has become an important restricting factor for livelihood development, thus exerting local social and economic impacts. Coal is the main fuel of the local community and also the main source of indoor pollution. This study aims to explore the coal combustion inhalable fine particulate matter (PM2.5) and its component output differences in different areas of Xuanwei, Yunnan. Moreover, the aim of this study is to investigate the relationship between inhalation of fine particles and high incidence of local lung cancer. Methods For combustion test, coal mines designated as C1, K7 and M30 were collected from LaoLin Colliery of Laibing Town, Huchang Colliery of Baoshan Town, and Taiping Colliery of Wenxing Town in Xuanwei, respectively. PM2.5 of indoor air was weighed, analyzed for elemental composition, and morphologically compared. The pathological specimen of lung cancer patients in Xuanwei who underwent operation was observed through electron microscope. Results The PM2.5 concentrations in indoor air were (8.244 ±1.460) mg/m3 (C1), (5.066±0.984) mg/m3 (K7), and (5.071±1.460) mg/m3 (M30). The differences among pairwise comparisons were statistically significant (P=0.029). The filter impurities of C1 coal seam primarily include Si- and O-enriched compounds. Moreover, three membranes that comprised other elements, including C, S, and Si, were observed. These membranes were evident from the aggregation of silica and a Ca-Al membrane. Compared with that of other coal seams, C1 coal generated a mass of impurities, in which several particles have irregular shape. We found nanoscale fine particles in some specimens of Xuanwei lung cancer patients. Conclusion The produced combustion of C1 coal was different from that of K7 and M30 coal. PM2.5 composition may be associated with the high local incidence of lung cancer.
Study of the Changes on Tree Shrew Bronchial Epithelium ?Induced by Xuanwei Bituminous Coal Dust
Xiaobo CHEN, Meng HE, Guangjian LI, Yongchun ZHOU, Guangqiang ZHAO, Yujie LEI, Kaiyun YANG, Linwei TIAN, Yunchao HUANG
- , 2015, DOI: : 10.3779/j.issn.1009-3419.2015.08.01
Abstract: Background and objective Lung cancer is the type of cancer with the highest incidence and mortality in numerous countries and regions. Establishing an appropriate animal model that can be used to simulate lung cancer etiology, pathogenesis, and similar processes, is urgent. We explore the feasibility of establishing a lung cancer model induced by Xuanwei bituminous coal dust PM10 (particulate matter with diameters of 10 μm or less), which affects bronchial epithelium of tree shrews. Methods The neck skin of adult tree shrews is dissected, and the thyroid cartilage is fully exposed. Subsequently, the weak part at the top of the thyroid cartilage is treated with intratracheal agents by perfusion via a special infusion needle puncture method. Regular X-ray examination and lung tissue biopsy were performed on the sacrificed animals to observe changes in pulmonary imaging and bronchial epithelial cells after perfusion of Xuanwei bituminous coal dust PM 10. Results The tree shrews of the experimental group (exposed to bituminous coal dust) died in a week after perfusion with PM10, whereas no animal died until the end of the experiment in the blank control and the solvent control groups. Sections of lung tissue biopsy of the regularly killed tree shrews were stained with hematoxylin and eosin. The lung tissues of tree shrews in the experimental group showed a serial changes caused by bronchial epithelial hyperplasia, such as squamous metaplasia, dysplasia, and early invasive carcinoma, whereas no significant pathological changes were observed in the blank control and solvent control groups. Conclusion Endotracheal infusion of Xuanwei bituminous coal dust PM10 induces lung cancer in tree shrews. Thus, the lung cancer model was established.
Crowdsourcing Spatio-Temporal Data Model Considering Reputation

周晓光, 赵肄江, 李光强, 张攀
ZHOU Xiaoguang
, ZHAO Yijiang, LI Guangqiang, ZHANG Pan

- , 2018, DOI: 10.13203/j.whugis20150378
Abstract: 提出了一种顾及信誉度的众源时空数据模型。在分析众源时空数据中地理要素、目标状态、对象版本、贡献者、信誉度、改变现实空间实体或信息空间对象状态的事件等要素间的相互作用机理的基础上,采用面向对象方法设计了一种顾及信誉度的众源时空数据组织方法,用UML对其进行描述,分析了与信誉度相关操作及其联动关系,得出了8条联动规则。开发了顾及信誉度的众源时空数据管理原型系统,验证了所提模型的有效性
Large deviation principle of SDEs with non-Lipschitzian coefficients under localized conditions
Yunjiao Hu,Guangqiang Lan
Mathematics , 2014,
Abstract: Localized sufficient conditions for the large deviation principle of the given stochastic differential equations will be presented for stochastic differential equations with non-Lipschitzian and time-inhomogeneous coefficients, which is weaker than those relevant conditions existing in the literature. We consider at first the large deviation principle when $\int_0^t\sup_{x\in\mathbb{R}^d}||\sigma(s,x)||\vee|b(s,x)|ds=:C_t<\infty$ for any fixed $t$, then we generalize the conclusion to unbounded case by using bounded approximation program.
Exponential stability of the exact solutions and $θ$-EM approximations to neutral SDDEs with Markov switching
Guangqiang Lan,Chenggui Yuan
Mathematics , 2014,
Abstract: Exponential stability of the exact solutions as well as $\theta$-EM ($\frac{1}{2}<\theta\le 1$) approximations to neutral stochastic differential delay equations with Markov switching will be investigated in this paper. Sufficient conditions are obtained to ensure the $p$-th moment ($p\ge1$) and almost sure exponential stability of the exact solutions as well as $\theta$-EM approximations ($p=2$). An example will be presented to support our conclusions.
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