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OALib Journal期刊

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Estructura de la comunidad de macroinvertebrados del arroyo Achiras (Córdoba, Argentina): análisis previo a la construcción de una presa
Gualdoni, Cristina Mabel;Oberto, Ana M.;
Iheringia. Série Zoologia , 2012, DOI: 10.1590/S0073-47212012000200010
Abstract: regulation of rivers and streams modifies parameters that directly influence on the biota. in achiras-del gato endorheic basin has completed the construction of a midsize dam that will alter the limnological characteristics of the system and the structure of benthic communities. this study provides the first taxonomic records of macrobenthos of this lotic system and analyzes the spatial and temporal variations of the community structure in the foothills stretches of achiras stream. zoobenthos samples were collected and abiotic variables were recorded in runs and riffles, during a year, in two sites located upstream and downstream of the dam area, prior to the dam was built. for each site, habitat and season we determined the abundance, frequency, taxonomic richness, shannon and evenness, and the data were analyzed using one-way and three-way anova. attributes of the reference communities were complemented by the determination of ecological indicators and detectors, and exclusive taxa. in the first site 73 taxa were recorded, simulium was the most abundant and leptohyphes eximius eaton, 1882, was the most frequent. in the second site we found 75 taxa, caenis was the most abundant and cricotopus sp. 3, was the most frequent. in this study composition and distribution of the biota and indicators taxa are discussed. we determined that the community attributes were affected by the hydrological periods in wich the samples were taken. achiras stream has a phreatic-pluvial hydrological regime with low flows in winter and high flows in summer. therefore, we expect changes in native biota and in system integrity in response to hydraulic variability reduction when achiras dam start functioning and alters the natural flow.
Estado ecológico de dos arroyos serranos del sur de Córdoba, Argentina
Gualdoni,Cristina M; Duarte,Claudia A; Medeot,Erica A;
Ecolog?-a austral , 2011,
Abstract: the development of methodology that allows us to assess the environmental deterioration of fluvial systems from the biotic components has been of high attention in the last time. with the aim to evaluate the ecological state of two mountains streams in sections that cross communes of tourist interest, indexes of water and riparian forest quality were combined. in each stream, two stations were selected, one downstream and the other upstream of the recreation areas. environmental data were registered and benthos samples were collected in the main geomorphological units, during high and low water periods. metric indexes and índice biótico carcara?á (ibc) based on benthic macroinvertebrates were calculated, and the marginal forest quality was determined by the riparian quality index (qbr). the results were integrated by a modification of index ecostrimed to evaluate the ecological status of the fluvial system. in both streams, the physico-chemical variables showed normal values and the metric indexes determined that the water quality vary between "moderately deteriorated" and "little deteriorated", whereas the ibc revealed a "unpolluted environment" in all sampling situations. application of qbr showed that the riparial forest with important alterations and extreme degradation were located close to urban areas, while in the other reaches the alteration degree was translated in judgments of acceptable and good quality. when combining the results of the metric indexes with those of the qbr, in both streams were obtained quality judgments that indicated "good" and "intermediate" ecological status in the stretches located upstream of urban areas and "intermediate" in the stretches downstream of recreation areas. the results of this study contribute to corroborate that, from a biological perspective; the application of a set of metric is the most efficient and economic methodology, to evaluate the quality of the water since they integrate information derived from diverse as
Low nasal carriage of drug-resistant bacteria among medical students in Vienna
Guido A. Gualdoni,Tilman Lingscheid,Selma Tobudic,Heinz Burgmann
GMS Krankenhaushygiene Interdisziplin?r , 2012,
Abstract: Background: Multi-drug resistant bacteria are increasing and remain a major public health challenge worldwide. In order to understand the potential role of medical students as a reservoir for circulating pathogenic bacteria and their transmission, we analysed the nasal colonisation among 86 clinically exposed medical students of the Medical University of Vienna, which is integrated into General Hospital of Vienna. Methods: Nasal swabs obtained from 79 students were eligible for further analysis. Nasal swabs were analysed for Gram-positive and Gram-negative bacteria with special emphasis on methicillin-resistant Staphylococcus aureus. Results: 25.3% of participants were positive for Staphylococcus aureus colonization; none of the isolates showed methicillin-resistance or expression of Pantoin-Valentine-leukocidin. However, 2.5% were positive for methicillin-resistant Staphylococcus epidermidis. No participant showed Streptococcus pneumoniae colonisation. Furthermore, 10.1% of the samples displayed growth of Gram-negative bacteria, yet none showed any relevant drug-resistance. Conclusion: In conclusion, our investigation did not reveal any clinically relevant multi-drug resistant bacterial colonisation among clinically exposed medical students in Vienna. This might be explained by well-established hygienic precautions or comparably low circulation of resistant bacteria.
Rac1 and Rac3 GTPases Regulate the Development of Hilar Mossy Cells by Affecting the Migration of Their Precursors to the Hilus
Roberta Pennucci, Stefania Tavano, Diletta Tonoli, Sara Gualdoni, Ivan de Curtis
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024819
Abstract: We have previously shown that double deletion of the genes for Rac1 and Rac3 GTPases during neuronal development affects late developmental events that perturb the circuitry of the hippocampus, with ensuing epileptic phenotype. These effects include a defect in mossy cells, the major class of excitatory neurons of the hilus. Here, we have addressed the mechanisms that affect the loss of hilar mossy cells in the dorsal hippocampus of mice depleted of the two Rac GTPases. Quantification showed that the loss of mossy cells was evident already at postnatal day 8, soon after these cells become identifiable by a specific marker in the dorsal hilus. Comparative analysis of the hilar region from control and double mutant mice revealed that synaptogenesis was affected in the double mutants, with strongly reduced presynaptic input from dentate granule cells. We found that apoptosis was equally low in the hippocampus of both control and double knockout mice. Labelling with bromodeoxyuridine at embryonic day 12.5 showed no evident difference in the proliferation of neuronal precursors in the hippocampal primordium, while differences in the number of bromodeoxyuridine-labelled cells in the developing hilus revealed a defect in the migration of immature, developing mossy cells in the brain of double knockout mice. Overall, our data show that Rac1 and Rac3 GTPases participate in the normal development of hilar mossy cells, and indicate that they are involved in the regulation of the migration of the mossy cell precursor by preventing their arrival to the dorsal hilus.
Spatial-temporal patterns of functional feeding groups in mountain streams of Córdoba, Argentina
Príncipe,Romina E; Gualdoni,Cristina M; Oberto,Ana M; Raffaini,Graciela B; Corigliano,María C;
Ecolog?-a austral , 2010,
Abstract: trophic structure of benthic communities is influenced by the availability of food resources which indeed may be conditioned by stream size, shading and substrate. this study aims to analyze the distribution of macroinvertebrate functional feeding groups in different habitats of mountain streams (córdoba, argentina) and to assess the environmental variables conditioning this distribution at the habitat level. four streams were sampled in two hydrological periods (high and low discharge) and three benthic samples were taken in riffles and runs of coarse and fine substrate. gathering collectors were dominant in most of the habitats, streams and periods except in riffles during the low water period in which filtering collectors dominated. at the habitat level, current velocity, substrate, abundance of macroalgae and twigs and leaves were the most important variables explaining functional feeding group distribution. functional feeding group abundances varied in relation to the stream, the hydrological period and the habitat. the dominance of collectors demonstrates the importance of the role of this functional group and that fine detritus is the main food resource in these lotic ecosystems. the phenology and life history of the species, and the amount and type of organic matter retained in each habitat may explain the observed spatial-temporal patterns.
Immunogenicity and Tolerability after Two Doses of Non-Adjuvanted, Whole-Virion Pandemic Influenza A (H1N1) Vaccine in HIV-Infected Individuals
Heimo Lagler, Katharina Grabmeier-Pfistershammer, Veronique Touzeau-R?mer, Selma Tobudic, Michael Ramharter, Judith Wenisch, Guido Andrés Gualdoni, Monika Redlberger-Fritz, Theresia Popow-Kraupp, Armin Rieger, Heinz Burgmann
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036773
Abstract: Background During the influenza pandemic of 2009/10, the whole-virion, Vero-cell-derived, inactivated, pandemic influenza A (H1N1) vaccine Celvapan? (Baxter) was used in Austria. Celvapan? is adjuvant-free and was the only such vaccine at that time in Europe. The objective of this observational, non-interventional, prospective single-center study was to evaluate the immunogenicity and tolerability of two intramuscular doses of this novel vaccine in HIV-positive individuals. Methods and Findings A standard hemagglutination inhibition (HAI) assay was used for evaluation of the seroconversion rate and seroprotection against the pandemic H1N1 strain. In addition, H1N1-specific IgG antibodies were measured using a recently developed ELISA and compared with the HAI results. Tolerability of vaccination was evaluated up to one month after the second dose. A total of 79 HIV-infected adults with an indication for H1N1 vaccination were evaluated. At baseline, 55 of the 79 participants had an HAI titer ≥1:40 and two patients showed a positive IgG ELISA. The seroconversion rate was 31% after the first vaccination, increasing to 41% after the second; the corresponding seroprotection rates were 92% and 83% respectively. ELISA IgG levels were positive in 25% after the first vaccination and in 37% after the second. Among the participants with baseline HAI titers <1:40, 63% seroconverted. Young age was clearly associated with lower HAI titers at baseline and with higher seroconversion rates, whereas none of the seven patients >60 years of age had a baseline HAI titer <1:40 or seroconverted after vaccination. The vaccine was well tolerated. Conclusion The non-adjuvanted pandemic influenza A (H1N1) vaccine was well tolerated and induced a measurable immune response in a sample of HIV-infected individuals.
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