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Search Results: 1 - 10 of 3176 matches for " Guadalupe; Trejo-Tapia "
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Propagation and conservation of Castilleja tenuiflora Benth. ("hierba del cancer") through in vitro culture
Salcedo-Morales, Guadalupe;Rosas-Romero, Gabriel;Nabor-Correa, Nayeli;Bermúdez-Torres, Kalina;López-Laredo, Alma R.;Trejo-Tapia, Gabriela;
Polibotánica , 2009,
Abstract: we undertook this study to (1) evaluate an in vitro procedure for plantlet regeneration of castilleja tenuiflora benth. (scrophulariaceae) from axillary buds and (2) induce callugenesis and organogenesis through the manipulation of explant type, culture media and plant growth regulators. an efficient propagation protocol for in vitro multiplication and plantlet regeneration of c. tenuiflora using axillary buds of wild plants was developed. shoot multiplication was induced from axillary buds in murashige and skoog (ms) medium containing 0.2 mg l-1 bap and 0.1 mg l-1 naa with an efficiency of 33%. shoot multiplication and elongation were achieved in one step using 0.1 mg l-1 iba and 0.25 mg l-1 bap. after 14 days, an average of four shoots per explant was observed. for rooting, iba was increased to 1.0 mg l-1 and bap was excluded. hyperhydricity was not observed and 88% of the shoots rooted. from one axillary bud, 250 plantlets were produced within eight weeks. to induce callugenesis and organogenesis, explants (leaves and internodes) from plantlets were excised and inoculated into ms, b5 and nn culture media in combination with naa (0-10 μm) and kinetin (0-0.5 μm). in general, rhizogenesis was the main in vitro response (up to 100%) followed by shoot formation (5-50%) and, finally, callugenesis (2-35%). internodes were more competent than leaves for both callugenesis and organogenesis, along with the fact that leaf explants oxidized easily. rhizogenesis depended on exogenous naa, but auxin requirement varied according to the culture medium and type of explant used. on the basis of our results, conditions for callugenesis and organogenesis induction of c. tenuiflora can be recommended: a) callus-internode, 0.1 μm naa and b5 medium; b) rhizogenesis-0.1 μm naa and nn medium; and c) shoots-internode, 0.1 μm naa and ms medium. results of the present study show the feasibility of using in vitro culture to propagate and conserve germplasm of the 'cancer herb' c. tenuiflora.
Establecimiento de un protocolo de permeabilización con detergentes que incrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L
Salcedo-Morales,Guadalupe; Trejo-Tapia,Gabriela; Martínez-Bonfil,Blanca P; De Jesús-Sánchez,Antonia; Arenas-Ocampo,Martha L; Jiménez-Aparicio,Antonio R;
Interciencia , 2009,
Abstract: red beet (beta vulgaris l.) cell suspensions were permeabilized by means of four chemical detergent agents, tween 20, 40 and 80, and triton x-100?, to evaluate the recovery of betacianins (bc). the permeabilizating agent was selected as a function of the quantity of bc released and the contact time. betacianin concentration was measured using digital color image analysis. the results showed that 36% of betacianins was released using triton x-100? (0.7mm) during 10min; using these extraction conditions, the viability remained at 60-70%. this treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.
La agregación celular en la producción de metabolitos secundarios en cultivos vegetales in vitro
Trejo-Tapia,Gabriela; Rodríguez-Monroy,Mario;
Interciencia , 2007,
Abstract: plant cell culture represents a biotechnological alternative to direct extraction of whole plant for production of secondary metabolites. however, the productivity of in vitro systems is lower than that obtained from plants. this review illustratates cell differentiation and compartmentalization as necessary events for the biosynthesis of chemical compounds. the induction of in vitro cell aggregation is discussed as one of the strategies to stimulate the accumulation of the compounds of interest. this positive effect might be explained as a consequence of the development of morphogenic structures and/or a stress condition induced by oxygen limitation in the interior of the aggregates. finally, it is shown that the combination of aggregation with other strategies such as selection of cell lines, elicitation or precursor addition constitutes an alternative for the development of bioprocesses based on plant cell cultures for the production of high value chemical compounds
Oxygen limitations to grow Azadirachta indica cell culture in shake flasks
Orozco-Sanchez, F.;Sepúlveda-Jiménez, G.;Trejo-Tapia, G.;Zamilpa, A.;Rodríguez-Monroy, M.;
Revista mexicana de ingeniería química , 2011,
Abstract: it was evaluated the growth of azadirachta indica cell suspension in different conditions of oxygen delivery in erlenmeyer shake flask. oxygen transfer rate (otr, kg o2 m-3 day-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). a. indica cells growing during 6 weeks of subculture showed that lower otr reduced cell viability, the ph of broth medium, and azadirachtins production. while, higher otr induced the formation of aggregates. using a stirred tank bioreactor, it was determined that a. indica cells had an oxygen consumption of 0.100 kg o2 kg dw-1 day-1, a higher value than other plant cell cultures. these results show that otr generated in erlenmeyer s hake flasks is lower to oxygen uptake rate of a. indica cells and it is a limiting factor to grow this plant.
El discurso de A Favor de lo Mejor, A.C. un estudio de recepción
María Guadalupe Trejo Estrada
Estudios sobre las Culturas Contemporaneas , 2009,
Abstract: El artículo presenta resultados de una experiencia de evaluación del Curso de Recepción Crítica de A Favor de lo Mejor, A.C., y de otros materiales de esta organización, realizada en una localidad michoacana en el 2005. Se utilizaron, desde la perspectiva teórico-metodológica de los estudios culturales, la observación participante, el grupo de discusión y el análisis crítico del discurso para recoger e interpretar la ideología subyacente al curso de referencia, así como la manera particular de decodificar y resignificar dicha ideología por parte de un grupo de madres de familia de origen otomí en el ejido Jesús del Monte, municipio de Morelia. Este enfoque develó, asimismo,los sentidos que estas mujeres otorgan al uso y a los contenidos de los medios de comunicación, así como las características de su relación y la de sus hijos en edad escolar con aquéllos. Las recomendaciones finales subrayan la importancia de sustituir la estructura comunicacional del curso (dise ado para un modelo de tipo instruccional), por una que permita la reflexión y construcción del conocimiento a partir de la riqueza cultural y experiencial de los participantes en él.
Números cromosómicos de Asteraceae de la Reserva Ecológica del Pedregal de San ángel (REPSA), México, Distrito Federal
Soto-Trejo, Fabiola;Palomino, Guadalupe;Villase?or, José Luis;
Revista mexicana de biodiversidad , 2011,
Abstract: mitotic or meiotic chromosome numbers are recorded for 31 species of asteraceae growing in the ecological reserve of the pedregal de san angel (repsa), mexico city. the chromosome counts of 3 species endemic to mexico are recorded for the first time: acourtia cordata (2n= 54), ageratina cilyndrica (2n= 34), and viguiera buddleiiformis (n= 17). the results are discussed in relation to the available data for each of the analyzed taxa.
Obtención y caracterización de combustible sólido (RDF) a partir de los residuos sólidos urbanos de una ciudad mexicana
Rodolfo Trejo Vázquez,Ma. Guadalupe Morales Cervantes
Revista internacional de contaminación ambiental , 1989,
Abstract: Sc presenta un cstudio experimental a nivel de laboratorio del proceso de obtención y caractcrizaci6n dc combustible sólido almacenable (conocido internacionalmcnte como RDF), logrado a partir de los residuos sólidos urbanos de una ciudad mexicana. Sc analizó el efecto de las variaciones en la composición del RDF sobre algunas de sus propicdadcs como dcnsidad, consistencia, contcnido de ccniias, podcr. calorífico y tiempo mínimo de residencia en el horno para combusii6n total. Los resultados y conclusiones alcanzados no son solamente válidos para csta investigación, sino que permiten visualizar la sensibilidad de cualquier RDF a su composición.
Números cromosómicos de Asteraceae de la Reserva Ecológica del Pedregal de San ángel (REPSA), México, Distrito Federal Chromosome numbers in Asteraceae of the Ecological Reserve of the Pedregal de San ángel (REPSA), Mexico City, Mexico
Fabiola Soto-Trejo,Guadalupe Palomino,José Luis Villase?or
Revista mexicana de biodiversidad , 2011,
Abstract: Se registraron los números cromosómicos mitóticos o meióticos de 31 especies de Asteraceae en la Reserva Ecológica del Pedregal de San ángel (REPSA), y por primera vez, los conteos cromosómicos de 3 especies endémicas de México: Acourtia cordata (2n= 54), Ageratina cylindrica (2n= 34) y Viguiera buddleiiformis (n= 17). Se discuten los resultados obtenidos de acuerdo con los datos disponibles para cada especie analizada. Mitotic or meiotic chromosome numbers are recorded for 31 species of Asteraceae growing in the Ecological Reserve of the Pedregal de San Angel (REPSA), Mexico City. The chromosome counts of 3 species endemic to Mexico are recorded for the first time: Acourtia cordata (2n= 54), Ageratina cilyndrica (2n= 34), and Viguiera buddleiiformis (n= 17). The results are discussed in relation to the available data for each of the analyzed taxa.
Presence of Sargassum horneri at Todos Santos Bay, Baja California, Mexico: Its Effects on the Local Macroalgae Community  [PDF]
Giuliana I. Cruz-Trejo, Silvia E. Ibarra-Obando, Luis E. Aguilar-Rosas, Miriam Poumian-Tapia, Elena Solana-Arellano
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.617271
Abstract: To describe the annual cycle of Sargassum horneri in Mexican waters, we selected two sites differing in their degree of wave exposure and sediment type: Rincón de Ballenas (RB), and Rancho Packard (RP). From June 2009 to April 2010 we followed the seasonal changes in S. horneri density and biomass along two intertidal transects per site. The effects of this non-indigenous species on the local macroalgae community were assessed by comparing their species composition, density, biomass, species richness, and diversity index in quadrats with and without S. horneri. There were significant differences in S. horneri density and biomass between sites (P < 0.001). At RB the invasive alga density average was 2 ± 0.94 individual m-2, with a mean biomass of 4 ± 0.95 g DW m-2. At RP, S. horneri density average was 10 ± 0.96 individual m-2, and mean biomass of 102 ± 0.97 g DW m-2. At RB, the invasive alga promoted a significant reduction in the four selected structural variables, and the corticated macrophytes and the foliose functional forms were severely reduced. At RP, there were only marginally significant effects (P = 0.06) of S. horneri presence on the local macroalgae community, and higher density, biomass, and diversity values were found when S. horneri was present. Most of the functional forms were found, even if the invasive alga was present. At both locations, the highest biomass corresponded to the articulated calcareous functional form. These contrasting results could be due to the fact that the native macroalgae community has already been altered by the early invasion of S. muticum, with the most resilient species and functional forms remaining in place. One of the most important changes we noticed is the severe reduction of the canopy forming species at both sites.
Diferencias sociales de la detección oportuna de cáncer cérvico uterino en las mujeres trabajadoras de una universidad de la ciudad de México
Trejo Amador,Ulises; Granados Cosme,José Arturo; Ortiz Hernández,Luis; Delgado Sánchez,Guadalupe;
Revista Espa?ola de Salud Pública , 2005, DOI: 10.1590/S1135-57272005000300008
Abstract: background: in mexico, cervical uterine cancer (cuc) is one of the leading causes of death among women, however a low degree of participation in the early detection programs has been found. this study is aimed at describing the social determinants of knowledge related to cervical uterine cancer and proper testing being conducted by establishing the social differences with regard thereto among a population of female employees at a public university in mexico. methods: an analytical cross-sectional study was made. during the march-april 2003 period, a survey was conducted of a representative sample of the female employees who were working at a public university (n=228), 110 of whom were professors (pf) and 118 administrative staff (as). this population was chosen due to the major socioeconomic differentiation thereof in terms of their job positions (pf versus as), thus affording the possibility of evaluating the bearing social inequality has on health-related behavior. the survey explored three aspects: living conditions, knowledge of cuc and of proper cervical uterine cancer detection testing. results: the female professors showed higher levels of income, schooling and knowledge of cuc and of cervical uterine cancer detection testing than the administrative staff. income and schooling were positively related to the knowledge regarding cervical uterine cancer, cervical uterine cancer detection testing being related to the latter. conclusions: socioeconomic inequality among the women studied was reflected in differences in the degree of knowledge and in cervical uterine cancer detection testing being undergone. these differences are associated, above all, to the differences in the level of schooling.
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