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Search Results: 1 - 10 of 39 matches for " Grisolia "
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Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment
Palhares, Dario;Grisolia, Cesar Koppe;
Genetics and Molecular Biology , 2002, DOI: 10.1590/S1415-47572002000300005
Abstract: the frequencies of mitomycin c and cyclophosphamide-induced kidney and gill erythrocyte micronuclei were compared in tilapia rendalli and oreochromis niloticus. cyclophosphamide (cp) and mitomycin c (mmc) were previously used to investigate fish sensitivity to micronuclei induction in kidney erythrocytes. fish were treated with a single dose of cyclophosphamide (40 mg/kg) or mitomycin c (2 mg/kg). peripheral blood samples and kidney blood samples were obtained, 1, 2, 3, 5, 7, and 15 days after the intra-abdominal injection of mmc. t. rendalli showed to be more sensitive than o. niloticus to micronuclei production. both compounds were used at the maximum tolerated dose (mtd), and mitomycin c proved to be more toxic than cyclophosphamide. the frequency of micronucleated erythrocytes observed varied significantly among the treated individuals. the difference between the kidney and gill micronuclei frequencies was not significant. higher levels of micronucleated erythrocytes were observed in tilapia rendalli than in o. niloticus. t. rendalli produced erythrocytes with deformed nuclei, which o. niloticus did not.
In vitro and in vivo studies demonstrate non-mutagenicity of the herbicide metolachlor
Grisolia, Cesar Koppe;Ferrari, Iris;
Brazilian Journal of Genetics , 1997, DOI: 10.1590/S0100-84551997000300009
Abstract: the herbicide metolachlor was evaluated for genotoxic potential. metolachlor did not induce micronuclei in mice, however at 40 mg/kg it significantly decreased the percentage of polychromatic erythrocytes, which is a cytotoxic effect. metolachlor did not induce chromosomal aberrations in human lymphocytes in vitro, but 2.0 mg/ml culture medium resulted in cytotoxicity, decreasing the mitotic index significantly. the indirect exposure test was carried out by adding plasma from metolachlor-pretreated rats to the human lymphocyte cultures. there was no indication of clastogenicity by metolachlor metabolites. on the other hand, plasma of cyclophosphamide-pretreated rats had a significant clastogenic effect
Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment
Palhares Dario,Grisolia Cesar Koppe
Genetics and Molecular Biology , 2002,
Abstract: The frequencies of mitomycin C and cyclophosphamide-induced kidney and gill erythrocyte micronuclei were compared in Tilapia rendalli and Oreochromis niloticus. Cyclophosphamide (CP) and mitomycin C (MMC) were previously used to investigate fish sensitivity to micronuclei induction in kidney erythrocytes. Fish were treated with a single dose of cyclophosphamide (40 mg/kg) or mitomycin C (2 mg/kg). Peripheral blood samples and kidney blood samples were obtained, 1, 2, 3, 5, 7, and 15 days after the intra-abdominal injection of MMC. T. rendalli showed to be more sensitive than O. niloticus to micronuclei production. Both compounds were used at the maximum tolerated dose (MTD), and mitomycin C proved to be more toxic than cyclophosphamide. The frequency of micronucleated erythrocytes observed varied significantly among the treated individuals. The difference between the kidney and gill micronuclei frequencies was not significant. Higher levels of micronucleated erythrocytes were observed in Tilapia rendalli than in O. niloticus. T. rendalli produced erythrocytes with deformed nuclei, which O. niloticus did not.
DNA damage levels in systemic lupus erythematosus patients with low disease activity: An evaluation by comet assay  [PDF]
Thaís Muniz Montalv?o, Ana Luisa Miranda-Vilela, Mariana Matos Roll, Cesar Koppe Grisolia, Leopoldo Santos-Neto
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.327121
Abstract: Oxidative stress has been implicated in the inflammatory process of Systemic Lupus Erythematosus (SLE), particularly by the formation of anti-DNA autoantibodies, which can lead to DNA damage. The aim of this study was to investigate, through comet assay, whether the level of DNA damage in SLE patients is different from that of healthy subjects. Twenty-five adult SLE patients with SLEDAI up to ten, and 25 healthy subjects were paired according to age, gender and Body Mass Index (BMI). Other anthropometric variables were also assessed. Comet assay was assessed as the marker of oxidative stress described as DNA Damage (DD) percentage. Waist Circumference (WC), Hip Circumference (HC) and BMI were also performed. Exclusion criteria for patients and controls comprised smoking and other chronic disorders. Level of damage index was remarkably higher in SLE patients than in controls, and no significant differences between the groups were found for age, BMI, WC and HC. No stratification concerning gender was performed, since there were just two males per group. No correlation was observed between BMI and DD (%). DD increased in SLE, which reflects the oxidant/antioxidant imbalance in these patients. These findings support an association between oxidative stress and SLE. This stronger correlation observed in patients with low disease activity may be useful in elucidating the mechanisms of disease pathogenesis.
Toxicity and genotoxicity of wastewater from gasoline stations
Oliveira-Martins, Cynthia R.;Grisolia, Cesar K.;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000094
Abstract: the toxicity and genotoxicity of wastewater from eight gasoline stations in brasília, brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in allium cepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (oreochromis niloticus). the content of gasoline station effluents was also analyzed based on several physico-chemical parameters. none of the wastewater samples was genotoxic to a. cepa root cells, although cell proliferation was significantly inhibited, especially at the highest concentrations. likewise, no micronuclei were observed in o. niloticus peripheral erythrocytes, even after exposure to high concentrations, but there was an increase in the number of nuclear abnormalities and fish mortality. these results show that although the effluent from gasoline stations is processed by an oil/water separation system before being discharged into the main sewage system, the wastewater still contains toxic compounds.
Variability in micronucleus induction with different mutagens applied to several species of fish
Grisolia, Cesar Koppe;Cordeiro, Célia Maria Torres;
Genetics and Molecular Biology , 2000, DOI: 10.1590/S1415-47572000000100041
Abstract: fish are often used for screening genotoxicity of water. for such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. to investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, tilapia rendalli, oreochromis niloticus and cyprinus carpio, were exposed to the clastogens bleomycin (blm), cyclophosphamide (cp), 5-fluorouracil (5-fu), and mitomycin c (mmc). the binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin b was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes. micronucleus frequencies induced by cp were significantly greater than their respective controls for the three fish species throughout all treatment periods. during the whole evaluation period (30 days) cp was also the most effective clastogen. in general, until the 14th day of evaluation period t. rendalii was the most sensitive species to clastogens. no difference in micronucleus frequencies among species was observed in the 4th evaluation (at the 30th day). a micronucleus peak was observed at the 7th day after treatment. after the 14th day the frequencies were stabilized. the cytochalasin b experiment was carried out to demonstrate that micronuclei induced in the young kidney erythrocyte cells were detected in the circulating blood 2-4 days later.
La percepción de la violencia entre pares en contextos escolares: un estudio cualitativo
Veccia,Teresa Ana; Calzada,Javier Gonzalo; Grisolia,Erika Valeria;
Anuario de investigaciones , 2008,
Abstract: the violence has increased in different levels and countries during the last years. this problem is caused by historic, institutional, family and personal components. in this article we focused on the violence in schools and more specific the violence among children. this kind of maltreatment is a perturbation of the relationship among the members of a group, and is an expression of the interpersonal violence. when these aggressiveness relationships become in a rigid configuration dominate-dominated and persist in time, these are known as bullying. these type of behaviors bring serious psychological and social consequences. this article has used a qualitative methodology and has the aim of understanding and interpreting the reality of bullying from the perception of the adults who belong to one of the schools where the research has taken place.
Características físicas e químicas do habitat da Biomphalaria tenagophila (Mollusca, Planorbidae)
Grisolia, Maria de Lourdes Machado;Freitas, José Rabelo de;
Memórias do Instituto Oswaldo Cruz , 1985, DOI: 10.1590/S0074-02761985000200016
Abstract: samples of water and snails collected through aleatory scoops in a small dam were done to obtain data concerning the physical and chemical characteristics of the water and their possible influence on biological aspects of the life cycle of snails. among the 17 analysed parameters, alkalinity and conductivity showed a positive correlation with the density of b. tenagophila (r = +0.224 and r = +0.290), while co2 and acidity were negatively correlated with this populacional parameter (r = -0.592 and 0.601). alkalinity and total hardness values were slightly higher than 100 mg/l caco3. chlorides and conductivity showed means of 94.9 ± 38.7 and 680.1 ± 643 micros/cm; these values are very high for that region. other factors like ph and od are according to the pattern for provision untreated waters. the density of b. tenagophila declined in the 6th month after a long period of torrential rains and in the rainy summers. in the colder months of the following year the density was higher (until 124 individuals/months/90 scoops). the monthly mean of the diameters of the snails was always larger than 13 mm, reaching 21.4 ± 4.1 mm, but the mode was about 17 mm. there was no correlation between diameter/density (r = 0.037) and density/temperature (r = 0.065).
Variability in micronucleus induction with different mutagens applied to several species of fish
Grisolia Cesar Koppe,Cordeiro Célia Maria Torres
Genetics and Molecular Biology , 2000,
Abstract: Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, Tilapia rendalli, Oreochromis niloticus and Cyprinus carpio, were exposed to the clastogens bleomycin (BLM), cyclophosphamide (CP), 5-fluorouracil (5-FU), and mitomycin C (MMC). The binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin B was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes. Micronucleus frequencies induced by CP were significantly greater than their respective controls for the three fish species throughout all treatment periods. During the whole evaluation period (30 days) CP was also the most effective clastogen. In general, until the 14th day of evaluation period T. rendalii was the most sensitive species to clastogens. No difference in micronucleus frequencies among species was observed in the 4th evaluation (at the 30th day). A micronucleus peak was observed at the 7th day after treatment. After the 14th day the frequencies were stabilized. The cytochalasin B experiment was carried out to demonstrate that micronuclei induced in the young kidney erythrocyte cells were detected in the circulating blood 2-4 days later.
Brain network involved in visual processing of movement stimuli used in upper limb robotic training: an fMRI study
Nocchi Federico,Gazzellini Simone,Grisolia Carmela,Petrarca Maurizio
Journal of NeuroEngineering and Rehabilitation , 2012, DOI: 10.1186/1743-0003-9-49
Abstract: Background The potential of robot-mediated therapy and virtual reality in neurorehabilitation is becoming of increasing importance. However, there is limited information, using neuroimaging, on the neural networks involved in training with these technologies. This study was intended to detect the brain network involved in the visual processing of movement during robotic training. The main aim was to investigate the existence of a common cerebral network able to assimilate biological (human upper limb) and non-biological (abstract object) movements, hence testing the suitability of the visual non-biological feedback provided by the InMotion2 Robot. Methods A visual functional Magnetic Resonance Imaging (fMRI) task was administered to 22 healthy subjects. The task required observation and retrieval of motor gestures and of the visual feedback used in robotic training. Functional activations of both biological and non-biological movements were examined to identify areas activated in both conditions, along with differential activity in upper limb vs. abstract object trials. Control of response was also tested by administering trials with congruent and incongruent reaching movements. Results The observation of upper limb and abstract object movements elicited similar patterns of activations according to a caudo-rostral pathway for the visual processing of movements (including specific areas of the occipital, temporal, parietal, and frontal lobes). Similarly, overlapping activations were found for the subsequent retrieval of the observed movement. Furthermore, activations of frontal cortical areas were associated with congruent trials more than with the incongruent ones. Conclusions This study identified the neural pathway associated with visual processing of movement stimuli used in upper limb robot-mediated training and investigated the brain’s ability to assimilate abstract object movements with human motor gestures. In both conditions, activations were elicited in cerebral areas involved in visual perception, sensory integration, recognition of movement, re-mapping on the somatosensory and motor cortex, storage in memory, and response control. Results from the congruent vs. incongruent trials revealed greater activity for the former condition than the latter in a network including cingulate cortex, right inferior and middle frontal gyrus that are involved in the go-signal and in decision control. Results on healthy subjects would suggest the appropriateness of an abstract visual feedback provided during motor training. The task contributes to hig
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