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Search Results: 1 - 10 of 63 matches for " Granjeiro "
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Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products  [PDF]
M. N. P. Vidal, J. M. Granjeiro
Journal of Biomedical Science and Engineering (JBiSE) , 2017, DOI: 10.4236/jbise.2017.109033
Abstract: The risks and damages related to the use of products, technologies and services of sanitary interest can be due to defects or manufacturing failures. Certain products already contain a certain degree of risk, which requires strict quality control in their production, distribution and use, as well as in the disposal of their waste in the environment. With continuous development in science and technology, medical devices must undergo intradermal irritation and testing for sensitization, cytotoxicity, and acute systemic toxicity. In health care, biotechnology aims to provide technology-based products or processes related to energy, food, and health, which are capable of stimulating new businesses, expanding exports, integrating the value chain and stimulating new demands for innovative products and processes, taking into account health policies. The present article was prepared by a bibliographical survey of the electronic databases PubMed, Lilacs, and Bireme. Cell culture testing can be successfully employed, as it is reproducible, rapid, sensitive, and financially accessible for performing in vitro toxicity testing. Thus, it has been possible to optimize the development phase of new products by decreasing animal use or even replacing them in certain tests. Some in vitro assays validated by the Organization for Economic Cooperation and Development in the area of health products have already replaced animal testing.
Increase of gastric area and weight gain in rats submitted to the ingestion of gasified water
Santiago, José Roberto Ferreira;Kobayasi, Shoiti;Granjeiro, José Mauro;
Acta Cirurgica Brasileira , 2004, DOI: 10.1590/S0102-86502004000300007
Abstract: purpose: due to the progressive increasing in the use of gasified drinks and weight gain in the brazilian population, in addition to the fact that carbonic gas is present in all soft drinks, an experimental study was conducted using rats as the subject to investigate the effects of gasified water in the hydric ingestion and food intake, weight gain, gastric area, blood sugar, hematocrit, and hemoglobin. methods: four groups of 12 rats were studied for 36 days while receiving the following daily diet, four times per day: group 1 - 35g/day of rat food "ad libitum" and 20ml of non-gasified water; group 2 - 35g/day of rat food "ad libitum" and 20 ml of gasified water; group 3 - 10g/day of rat food "ad libitum" and 20ml of non-gasified water; and group 4 - 10g/day of rat food "ad libitum" and 20ml of gasified water. results: the results showed that the animals submitted to the treatment with gasified water (groups 2 and 4), presented a larger volume of hydric ingestion and significant increase of the gastric area (p<0,001). in group 2, the food intake and the weight gain were significant (p<0,01). blood sugar, hematocrit and hemoglobin data didn't show significant alterations among the studied groups. conclusion: the authors of this study concluded that gasified water favored the hydric ingestion, food intake, and weight gain, as well as expanded the gastric area.
Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
Conz, Marcio Baltazar;Granjeiro, José Mauro;Soares, Gloria de Almeida;
Journal of Applied Oral Science , 2011, DOI: 10.1590/S1678-77572011005000007
Abstract: objective: the physicochemical properties of hydroxyapatite (ha) granules were observed to affect the biological behavior of graft materials. the aim of this work was to analyze the tissue response of two ha granules with different crystallinity and ca/p ratio in vivo. material and methods: the ha granules were produced in the biomaterials laboratory (coppe/ufrj). the testing materials were ha granules presenting a ca/p molar ratio of 1.60 and 28% crystallinity (ha-1), and a ca/p molar ratio of 1.67 and 70% crystallinity (ha-2). both has were implanted into a critical-size calvaria rat defects. results: to note, in the control group, the bone defects were filled with blood clot only. descriptive and histomorphometric analyses after 1, 3, and 6 months postoperatively showed mild inflammatory infiltrate, mainly comprising macrophage-like and multinucleated giant cells, and an increase in the volume density of the fibrous tissues (p<0.05), which was in contrast to the similar volume density of the newly formed bone and biomaterials in relation to the control group. conclusion: thus, we concluded that ha-1 and ha-2 are biocompatible and non-degradable, and that crystallinity does not affect bone repair of critical size defects.
Physicochemical characterization of six commercial hydroxyapatites for medical-dental applicatons as bone graft
Conz, Marcio Baltazar;Granjeiro, José Mauro;Soares, Gloria de Almeida;
Journal of Applied Oral Science , 2005, DOI: 10.1590/S1678-77572005000200008
Abstract: the aim of this work was to characterize six hydroxyapatites in granular form for applications in medicine and dentistry as bone graft and to compare with manufacturers' specification. these samples were produced by four different manufacturers, and all of them are easily available in the brazilian market. physicochemical characterization was carried out by using electron microscopy (sem), x-ray diffraction (xrd), infrared spectroscopy (ftir) and surface area (bet) measurements. the results show that just one hydroxyapatite sample exhibited characterisitics in perfect agreement with the manufacturers' specifications. the studied parameters, namely crystallinity, surface area and composition may be used to estimate hydroxyapatite's biodegradability and as a criterion for quality control.
O estudo psicossocial forense como subsídio para a decis?o judicial na situa??o de abuso sexual
Granjeiro, Ivonete Araújo Carvalho Lima;Costa, Liana Fortunato;
Psicologia: Teoria e Pesquisa , 2008, DOI: 10.1590/S0102-37722008000200005
Abstract: this research aimed at deepen knowledge in the psychology/law interface, involving an intrafamiliar violence situation. we tried to know the way of thinking of the various actors who participated in the decisions related to a process of sexual abuse committed by a father against two daughters. the context was a criminal court and the method was the case study. the analysis of the results followed the guidelines of the in-depth hermeneutics. the results were discussed in accordance with three sets of comprehension of the forensic psychosocial study function: to whom it serves? , when it must be made? and what subsidizes the judicial process: the evaluation report or the study? we came to the conclusion that it is necessary to promote the interaction among the various actors who complement their decisions in order to increase the comprehension of the reality surrounding the situations of familiar violence so that the judicial context may participate in the promotion of citizenship in these cases.
Increase of gastric area and weight gain in rats submitted to the ingestion of gasified water
Santiago José Roberto Ferreira,Kobayasi Shoiti,Granjeiro José Mauro
Acta Cirurgica Brasileira , 2004,
Abstract: PURPOSE: Due to the progressive increasing in the use of gasified drinks and weight gain in the Brazilian population, in addition to the fact that carbonic gas is present in all soft drinks, an experimental study was conducted using rats as the subject to investigate the effects of gasified water in the hydric ingestion and food intake, weight gain, gastric area, blood sugar, hematocrit, and hemoglobin. METHODS: Four groups of 12 rats were studied for 36 days while receiving the following daily diet, four times per day: Group 1 - 35g/day of rat food "ad libitum" and 20ml of non-gasified water; Group 2 - 35g/day of rat food "ad libitum" and 20 ml of gasified water; Group 3 - 10g/day of rat food "ad libitum" and 20ml of non-gasified water; and Group 4 - 10g/day of rat food "ad libitum" and 20ml of gasified water. RESULTS: The results showed that the animals submitted to the treatment with gasified water (Groups 2 and 4), presented a larger volume of hydric ingestion and significant increase of the gastric area (p<0,001). In group 2, the food intake and the weight gain were significant (p<0,01). Blood sugar, hematocrit and hemoglobin data didn't show significant alterations among the studied groups. CONCLUSION: The authors of this study concluded that gasified water favored the hydric ingestion, food intake, and weight gain, as well as expanded the gastric area.
Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
Marcio Baltazar Conz,José Mauro Granjeiro,Gloria de Almeida Soares
Journal of Applied Oral Science , 2011,
Abstract: OBJECTIVE: The physicochemical properties of hydroxyapatite (HA) granules were observed to affect the biological behavior of graft materials. The aim of this work was to analyze the tissue response of two HA granules with different crystallinity and Ca/P ratio in vivo. MATERIAL AND METHODS: The HA granules were produced in the Biomaterials Laboratory (COPPE/UFRJ). The testing materials were HA granules presenting a Ca/P molar ratio of 1.60 and 28% crystallinity (HA-1), and a Ca/P molar ratio of 1.67 and 70% crystallinity (HA-2). Both HAs were implanted into a critical-size calvaria rat defects. RESULTS: To note, in the control group, the bone defects were filled with blood clot only. Descriptive and histomorphometric analyses after 1, 3, and 6 months postoperatively showed mild inflammatory infiltrate, mainly comprising macrophage-like and multinucleated giant cells, and an increase in the volume density of the fibrous tissues (p<0.05), which was in contrast to the similar volume density of the newly formed bone and biomaterials in relation to the control group. CONCLUSION: Thus, we concluded that HA-1 and HA-2 are biocompatible and non-degradable, and that crystallinity does not affect bone repair of critical size defects.
Use of acid phosphatase as biomarker during the castor bean seeds germination (ricinus communis)
Paulo Afonso Granjeiro,Carmen Ferreira Veríssima,Hiroshi Aoyama
Engenharia Ambiental : Pesquisa e Tecnologia , 2008,
Abstract: One of the main oil crop of prominent social and economic importance is to mamoneira (Ricinus communis L.); with countless application in the industry and agricultural. Broadly it distributed in Brazil; his cultivation can be an alternative of sustainability in the Brazilian northeast. It know the physiological and biochemical mechanisms of the germination they are important for the best utilization of the plant. The objective of this work was use acid phosphatase as biomarker during the germination. In the rough extract occurred the dosage of the activity for pNPP; Tyr-Pi and PPi; determination of protein and inorganic phosphatse. The peak of activity for pNPP was in the seventh day; for PPi and Tyr-Pi in the ninth and for PEP in the fifth. The concentration of protein increased according to the days of germination; with peak of activity in the eighth day; being coincidental with the peaks of the activities for the substrates. The content of inorganic phosphate diminished with the time of germination and after the third day occurred a fall accentuated of its concentration. We concluded that acid phosphatase is important for the germination of the seeds and his paper is related with the mobilization of inorganic phosphate; the main nutrients for the development.
Unveiling novel genes upregulated by both rhBMP2 and rhBMP7 during early osteoblastic transdifferentiation of C2C12 cells
Juan C Bustos-Valenzuela, Andre Fujita, Erik Halcsik, Jose M Granjeiro, Mari C Sogayar
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-370
Abstract: BMPs (bone morphogenetic proteins) are members of the TGFβ (transforming growth factor-β) super-family of proteins, which regulate growth and differentiation of different cell types in various tissues, and play a critical role in the differentiation of mesenchymal cells into osteoblasts. In particular, rhBMP2 and rhBMP7 promote osteoinduction in vitro and in vivo, and both proteins are therapeutically applied in orthopaedics and dentistry.Using DNA microarrays and RT-qPCR, we identified both previously known and novel genes which are upregulated by rhBMP2 and rhBMP7 during the onset of osteoblastic transdifferentiation of pre-myoblastic C2C12 cells. Subsequent studies of these genes in C2C12 and mesenchymal or pre-osteoblastic cells should reveal more details about their role during this type of cellular differentiation induced by BMP2 or BMP7. These studies are relevant to better understanding the molecular mechanisms underlying osteoblastic differentiation and bone repair.Bone formation and fracture repair depends on the expression and action of the bone morphogenetic proteins (BMPs), which are members of the transforming growth factor beta (TGF-beta) superfamily of dimeric, disulphide-linked growth factors, comprising more than 15 related proteins. In addition to a crucial role in osteogenesis, BMPs display a myriad of roles in cell proliferation, differentiation, migration and apoptosis, in different cell types [1]. Their role is essential at early phases of development and organogenesis, such as axial embryo determination [2], as well as in limb, eye and kidney development, such that ablation of these genes results in death at very early stages of development, as observed in knock-out mice [3]. In humans, recombinant BMP2 and BMP7 have gained attention in bone repair and in non-union spinal fractures due to their capacity to stimulate the differentiation of mesenchymal stem cells from the periosteum near the lesion site after migration and proliferation induced
The rabbit as an animal model for experimental surgery
Calasans-Maia, M?nica Diuana;Monteiro, Maria Lucia;áscoli, Fábio Oliveira;Granjeiro, José Mauro;
Acta Cirurgica Brasileira , 2009, DOI: 10.1590/S0102-86502009000400014
Abstract: the white new zealand rabbit (oryctolagus cuniculus) is frequently used as a model for in vivo studies. however, information on precautions when using this animal as an experimental model is limited. this review of the literature covers the gamut from the selection of the animal model all the way to its death, and describes procedures for transporting, raising, breeding, housing, administering anesthesia and handling so as to rationalize the utilization of this species while exploiting its unique characteristics. based upon the literature and our own experience with white new zealand rabbits, we conclude that the rabbit is an adequate model for experimental surgery.
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