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CURSO DE ESCRITURAPARA INVESTIGADORES: UNA EXPERIENCIA EN CIENCIAS DE LA SALUD
Beatriz Veracoechea F,Graciela Henríquez G
Salud, Arte y Cuidado , 2011,
Abstract: This article describes the experience of virtualizing an presential course, so the results of its usability. In 2009 began the process of virtualizing the subject: Writing Tools for Researchers in Health. The course was redesigned based on ASSURE model instructional plan. It involved the audience’s characteristics and skills analysis regarding to the use of ICT, as well as objective changing, means, methods, materials, and new strategies of learning assessment, so the virtual classroom edition on Moodle platform. Once the course was redesigned, it was offered to 17 professors of the Faculty of Health Science at University Lisandro Alvarado, who were professors and counselors in nursing research having ICT skills. The study found that participants thought that this mode was relevant, due to its efficacy as tutors’ labor. So, it is highly recommend to some other colleagues.
AULA VIRTUAL INTRODUCCIóN A LA COMPUTACIóN PROGRAMA DE ENFERMERíA DE LA UCLA
Henríquez Graciela,Ugel Eunice,Torrealba Keyla,Veliz, Roberto
Salud, Arte y Cuidado , 2011,
Abstract: A field research feasible project for the implementation of a virtual classroom to students from the second semester of Nursing Program in the course Introduction to Computing was performed. In the first diagnosis phase, the need for the implementation of a virtual classroom in that subject was determined. Information was collected by means of a questionnaire validated through expert opinions before being applied. In the second phase, a feasibility study determined the legal and institutional support framework of the project. In the third phase, taking into account the results of diagnostic and feasibility phases, a proposal was designed meeting the objectives of the program using multimedia strategies. The design and development of the proposal will result in: a) four trained teachers competent to assume the facilitator and/or tutor role at distance, b) a screening test to obtain general and specific information about the students and c) a subject updated program, educational guides as well as the design of a virtual classroom.
ELECTRODEPOSITION AND CHARACTERIZATION OF ZnX (X=Se, Te) SEMICONDUCTOR THIN FILMS
G. RIVEROS,H GóMEZ,R. HENRíQUEZ,R. SSHREBLER
Boletín de la Sociedad Chilena de Química , 2002,
Abstract: In present work we report the one step electrodeposition of ZnX (X = Se y Te) thin films in acid solution. In order to establish the appropriate conditions for the electrodeposition, a voltammetric and photovoltammetric study on different substrates was previously performed. The films were analyzed by different techniques (SEM, EDS, XRD and optical reflectance). The composition of the ZnTe films was very close to the stoichiometric one, instead, ZnSe films presented a selenium excess that can be eliminated with a proper annealing. Optical reflectance characterization of ZnSe and ZnTe samples grown on titanium gave direct band gaps values of 2.64 eV and 2.27 eV, respectively, in agreement with those reported in the bibliography. El presente trabajo describe la síntesis y la caracterización de películas delgadas de ZnX (X = Se y Te) obtenidas por electrodeposición a potencial constante en medio ácido. Previamente, se realizó un estudio voltamétrico y fotovoltamétrico sobre diferentes substratos conductores los que permitieron determinar las mejores condiciones para la electro obtención de estos compuestos. Las películas delgadas de ZnSe y de ZnTe fueron analizadas por diferentes técnicas (SEM, EDS, XRD y medidas ópticas). Las películas de ZnTe presentaron una composición muy cercana a la estequiometrica, en tanto que las de ZnSe presentaron un exceso de Se el cual puede ser eliminado por un adecuado tratamiento térmico. La caracterización óptica de ambos semiconductores depositados sobre titanio arrojó valores de ancho de banda prohibida de transición directa de 2,64 eV para ZnSe y 2,27 eV para ZnTe, muy cercanos a los aceptados en bibliografía.
Additional evidence of the facultative intracellular nature of the fish bacterial pathogen Piscirickettsia salmonis
Gómez,F; Henríquez,V; Marshall,SH;
Archivos de medicina veterinaria , 2009, DOI: 10.4067/S0301-732X2009000300011
Abstract: piscirickettsia salmonis is a highly virulent and contagious microorganism that affects net pen-reared salmonid fish worldwide since the last third of the past century, with little knowledge about its intracellular survival mechanisms. following a number of recent and non-conclusive reports which questioned its obligate intracellular condition, we decided to show additional evidence to challenge this well-established paradigm carrying on both basic biology as well as classical molecular experiments that confirm its facultative intracellular nature. in this report, we unequivocally demonstrate that the bacteria recovered from tissue culture amplification, in vitro grown agar plates or grown in liquid cultures, were the same organism and all of these isolates equally fulfils koch?s postulates. in addition, genomic and proteomic analyses confirmed that bacteria from different growth source conditions belonged to the same lf89 prototype strain as originally described by fryer in 1992. notwithstanding, growth of the bacteria both in cell-free media as well as in tissue culture cell lines were definitively suboptimal, and much more analysis are required to fully understand the biology of the pathogen. interestingly, we were able to grow the bacteria in liquid media but at very slow rate. we conclude that, in addition to previous reports, our results confirmed the facultative intracellular nature of this fish pathogen.
Additional evidence of the facultative intracellular nature of the fish bacterial pathogen Piscirickettsia salmonis Evidencia adicional de la naturaleza intracelular facultativa del patógeno bacteriano de peces Piscirickettsia salmonis
F Gómez,V Henríquez,SH Marshall
Archivos de medicina veterinaria , 2009,
Abstract: Piscirickettsia salmonis is a highly virulent and contagious microorganism that affects net pen-reared salmonid fish worldwide since the last third of the past century, with little knowledge about its intracellular survival mechanisms. Following a number of recent and non-conclusive reports which questioned its obligate intracellular condition, we decided to show additional evidence to challenge this well-established paradigm carrying on both basic biology as well as classical molecular experiments that confirm its facultative intracellular nature. In this report, we unequivocally demonstrate that the bacteria recovered from tissue culture amplification, in vitro grown agar plates or grown in liquid cultures, were the same organism and all of these isolates equally fulfils Koch’s postulates. In addition, genomic and proteomic analyses confirmed that bacteria from different growth source conditions belonged to the same LF89 prototype strain as originally described by Fryer in 1992. Notwithstanding, growth of the bacteria both in cell-free media as well as in tissue culture cell lines were definitively suboptimal, and much more analysis are required to fully understand the biology of the pathogen. Interestingly, we were able to grow the bacteria in liquid media but at very slow rate. We conclude that, in addition to previous reports, our results confirmed the facultative intracellular nature of this fish pathogen. Piscirickettsia salmonis es un microorganismo altamente contagioso y virulento que afecta a la salmonicultura mundial desde el último tercio del siglo pasado y del cual sus mecanismos de sobrevivencia intracelular son completamente desconocidos. Después de algunos reportes recientes en donde se cuestiona su condición de intracelular obligado, hemos decidido mostrar evidencia adicional para cambiar este paradigma, llevando a cabo experimentos tanto clásicos como moleculares que confirman su naturaleza de intracelular facultativo. En este reporte se demuestra inequívocamente que la bacteria recuperada desde cultivos celulares infectados, de placas de agar o de medio líquido, es el mismo organismo, el cual cumple con los postulados de Koch. Además, análisis genéticos y proteómicos confirman que la bacteria obtenida de diferentes fuentes de crecimiento corresponde a la misma cepa tipo LF-89, la que fue originalmente descrita por Fryer en 1992. Sin embargo, el crecimiento de la bacteria, tanto en medios libres de células como en cultivo celular, es subóptimo, por lo cual se requieren más análisis para entender completamente la biología del patógeno.
Margination regimes and drainage transition in confined multicomponent suspensions
R. G. Henríquez Rivera,K. Sinha,M. D. Graham
Physics , 2015, DOI: 10.1103/PhysRevLett.114.188101
Abstract: A mechanistic theory is developed to describe segregation in confined multicomponent suspensions such as blood. It incorporates the two key phenomena arising in these systems at low Reynolds number: hydrodynamic pair collisions and wall-induced migration. In simple shear flow, several regimes of segregation arise, depending on the value of a "margination parameter" M. Most importantly, there is a critical value of M below which a sharp "drainage transition" occurs: one component is completely depleted from the bulk flow to the vicinity of the walls. Direct simulations also exhibit this transition as the size or flexibility ratio of the components changes.
Búsqueda de la mutación delta F508 y análisis de dos polimorfismos de nucleótido único en el gen CFTR, en una muestra de población general de Valparaíso, Chile
Vera L,Alejandra; Henríquez-Roldán,Carlos F; González R,Francisco J; Molina F,Graciela;
Revista médica de Chile , 2005, DOI: 10.4067/S0034-98872005000700003
Abstract: background: the cystic fibrosis (cf) carrier rate in chile was estimated to be 1/40. cf is caused by mutations in the cystic fibrosis transmembrane conductance regulator (cftr) gene. delta f508 mutation is the most common in cf patients in chile and worldwide. delta f508 has linkage disequilibrium with two single nucleotide polymorphisms (snp), often used to define the haplotypic frameworks of cf mutations. aim: to know the frequency of the delta f508 mutation and to establish the snps, m470v and t854t, haplotypic frequency, in a valparaiso general population sample. subjects and methods: fifty subjects were studied. genetic material was obtained from blood samples, amplified by pcr and analyzed by restriction fragment length polymorphism. results: two of the 100 chromosomes analyzed, carried the delta f508 mutation. therefore, the observed frequency carrier rate (0.02) was higher than the expected (0.01). both carrier chromosomes had the same snps haplotypic framework (1-2). in normal chromosomes, the haplotype 2-1 was the most common. discussion: these results suggest that the chromosomes that bear delta f508 mutation have most likely a mediterranean european origin, since this haplotypic framework has been reported in that region. we suggest that cf could be more common in valparaiso than it was previously
Búsqueda de la mutación delta F508 y análisis de dos polimorfismos de nucleótido único en el gen CFTR, en una muestra de población general de Valparaíso, Chile Screening of the delta-F508 mutation and analysis of two single nucleotide polymorphism of the CFTR gene, in a sample of the general population of Valparaíso, Chile
Alejandra Vera L,Carlos F Henríquez-Roldán,Francisco J González R,Graciela Molina F
Revista médica de Chile , 2005,
Abstract: Background: The Cystic Fibrosis (CF) carrier rate in Chile was estimated to be 1/40. CF is caused by mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. Delta F508 mutation is the most common in CF patients in Chile and worldwide. Delta F508 has linkage disequilibrium with two Single Nucleotide Polymorphisms (SNP), often used to define the haplotypic frameworks of CF mutations. Aim: To know the frequency of the delta F508 mutation and to establish the SNPs, M470V and T854T, haplotypic frequency, in a Valparaiso general population sample. Subjects and Methods: Fifty subjects were studied. Genetic material was obtained from blood samples, amplified by PCR and analyzed by restriction fragment length polymorphism. Results: Two of the 100 chromosomes analyzed, carried the delta F508 mutation. Therefore, the observed frequency carrier rate (0.02) was higher than the expected (0.01). Both carrier chromosomes had the same SNPs haplotypic framework (1-2). In normal chromosomes, the haplotype 2-1 was the most common. Discussion: These results suggest that the chromosomes that bear delta F508 mutation have most likely a Mediterranean European origin, since this haplotypic framework has been reported in that region. We suggest that CF could be more common in Valparaiso than it was previously
Biosorption of Pb(II) by Thiobacillus ferrooxidans
Graciela Rodelo,Agustín Gómez,Arturo Ruiz Manríquez
Revista internacional de contaminación ambiental , 2002,
Abstract: Debido principalmente al valor comercial y al impacto que los metales pesados causan, se han realizado muchos estudios sobre la posibilidad de removerlos y recuperarlos de soluciones diluidas. Los métodos tradicionales para la remoción tienen varias desventajas cuando los metales están presentes en concentraciones menores de 100 mg/L. La biosorción, que implica el uso de materiales biológicos como adsorbentes, ha sido considerada como un método alternativo. En este trabajo, se estudió el efecto de variables como pH y tratamiento químico de la biomasa en la capacidad de biosorción de Pb(II) por Thiobacillus ferrooxidans, así como el tiempo de biosorción. Los resultados indican que pueden ser obtenidos hasta 443 mg de Pb(II)/g de biomasa seca cuando el sistema se trabaja a pH 5, 25 oC y las células se tratan con NaOH 0.075 M. También se encontró que el proceso ocurre en los primeros 30 minutos.
A Novel Liquid Medium for the Efficient Growth of the Salmonid Pathogen Piscirickettsia salmonis and Optimization of Culture Conditions
Mirtha Henríquez, Ernesto González, Sergio H. Marshall, Vitalia Henríquez, Fernando A. Gómez, Irene Martínez, Claudia Altamirano
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071830
Abstract: Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free) medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L?1 were obtained, about eight times higher than those reported for the blood containing medium. A 2- level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23–27°C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.
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