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Search Results: 1 - 10 of 1930 matches for " Graciela Caino "
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EL ANALISIS DEL DISCURSO DE PROFESORES UNIVERSITARIOS EN LA CLASE Analysis of the discourse of University professors in Class
Montserrat de la Cruz,Virginia Baudino,Graciela Caino,Rosario Ayastuy
Estudios Pedagógicos (Valdivia) , 2000,
Abstract: El trabajo se ocupa de las concepciones de ense anza de profesores universitarios, que se infieren del análisis de sus discursos en clase. Con este propósito se identifican los actos de habla y el tipo de conocimiento implicado en cada uno de ellos. El análisis se lleva a cabo sobre 12 clases desgrabadas de distintos profesorados: matemática, biología y educación física y del área pedagógica. Los resultados muestran diferencias en los actos de habla que se corresponden con las diferencias en las concepciones de ense anza. La ense anza orientada hacia la actividad del docente y la transmisión de conocimientos se llevan a cabo en la clase a través del predominio de actos de habla como expone, indaga, usa recursos didácticos y corrige; en cambio, la ense anza centrada en la actividad del alumno y la facilitación del acceso al conocimiento es la que utiliza entre los actos de habla: indaga, aclara, argumenta. This is a study of the discourse university professors use in their classes. The corpus consists of twelve recorded classes by professors of Math, Biology, Physical Education and the Pedagogical Area. We analyse the speech acts taking place and the kinds of knowledge implied in each of them. Results show differences in the speech acts coinciding with differences in the professors' teaching conceptions as revealed by their answers to a written interview. Professors sustaining conceptions of teaching focussed on teachers' activity and transmission of knowledge use speech acts as expose, inquire, use of didactical resources and correct. In contrast, professors sustaining teaching conceptions focusses in the pupils' activity and facilitation of knowledge use speech acts as inquire, clarify and argue.
EL ANALISIS DEL DISCURSO DE PROFESORES UNIVERSITARIOS EN LA CLASE
de la Cruz,Montserrat; Baudino,Virginia; Caino,Graciela; Ayastuy,Rosario; Ferrero,Teresa; Huarte,; Palacio,Mónica; Reising,Ailín; Scheuer,Nora; Siracusa,Paula;
Estudios pedagógicos (Valdivia) , 2000, DOI: 10.4067/S0718-07052000000100001
Abstract: this is a study of the discourse university professors use in their classes. the corpus consists of twelve recorded classes by professors of math, biology, physical education and the pedagogical area. we analyse the speech acts taking place and the kinds of knowledge implied in each of them. results show differences in the speech acts coinciding with differences in the professors' teaching conceptions as revealed by their answers to a written interview. professors sustaining conceptions of teaching focussed on teachers' activity and transmission of knowledge use speech acts as expose, inquire, use of didactical resources and correct. in contrast, professors sustaining teaching conceptions focusses in the pupils' activity and facilitation of knowledge use speech acts as inquire, clarify and argue.
Comparación de dos técnicas de medición de peso en ni?os menores de cuatro a?os
Caino,Silvia; Adamo,Paula; Abeyá-Gilardon,Enrique O; Lejarraga,Horacio;
Archivos argentinos de pediatr?-a , 2006,
Abstract: introduction. weight is a widely used anthropometric measurement as indicator of health and nutritional status in children. usually, an appropriate plate scale is used to weigh infants (direct weight) but in some occasions, it is necessary to measure weight in an indirect way. indirect weight is measured as the difference between the weight of the child held up by the mother and the actual weight of the mother alone. objective. to compare the two techniques of weight measurement (direct and indirect) in children younger than four years of age. population. a convenience sample of children younger than 4 years attending at hospital of pediatría "prof. dr. juan p. garrahan". material and methods. the same observer measured each child's weight with the direct method on a mechanical scale for infants at the laboratory of anthropometry (precision, 10 g) and with the indirect method on an electronic scale (precision, 50 g). both scales were previously calibrated. the intra-observer measurement error was of 37 g in the electronic scale (cv 0.06%) and 4.6 g in the mechanical plate scale (cv 0.05%). arithmetic mean, ci 95%, sd and cv of the differences between the direct method and the indirect method were calculated. a paired student t test was performed. results. 49 children between 0.17 and 3.97 years of age participated in the study (weight range: 3,61-15.55 kg). the observed average difference between both methods (direct-indirect) was of 26.5 grams (±106.6 g), cv 0.58%, ic95% 10.7-42.4 g. this difference was statistically significant (p: 0.0015). conclusions: weight measurement with an indirect technique underestimated the weight of child as compared with the direct technique. the importance of this difference will depend on the precision needed for evaluating the weight measurement.
Pacto em defesa da saúde: divulgando os direitos dos usuários pela pesquisa-a??o
Koerich, Magda Santos;Backes, Dirce Stein;Marchiori, Mara Caino;
Revista Gaúcha de Enfermagem , 2009, DOI: 10.1590/S1983-14472009000400014
Abstract: we objective to discuss the users' rights booklet of the health unified system with patients and family members who had looked for attendance in public hospital service. one has chosen the action research, a qualitative and constructive method, which takes into account collective participation and planned action for changes of situations. the data were collected along with the research participants' discussions by means of weekly meetings throughout 2007. the codified speeches were analyzed and resulted in three themes: rights and duties of healthcare users; qualification of health professionals; receptivity to healthcare users. the users' rights booklet consists of an important means for citizens to know their rights of access to the healthcare system, allowing them to contribute in a critical and co-responsible way to discussions and actions in favor of a better healthcare system.
Regulation of Transcriptional Networks by PKC Isozymes: Identification of c-Rel as a Key Transcription Factor for PKC-Regulated Genes
Rachana Garg, M. Cecilia Caino, Marcelo G. Kazanietz
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067319
Abstract: Background Activation of protein kinase C (PKC), a family of serine-threonine kinases widely implicated in cancer progression, has major impact on gene expression. In a recent genome-wide analysis of prostate cancer cells we identified distinctive gene expression profiles controlled by individual PKC isozymes and highlighted a prominent role for PKCδ in transcriptional activation. Principal Findings Here we carried out a thorough bioinformatics analysis to dissect transcriptional networks controlled by PKCα, PKCδ, and PKCε, the main diacylglycerol/phorbol ester PKCs expressed in prostate cancer cells. Despite the remarkable differences in the patterns of transcriptional responsive elements (REs) regulated by each PKC, we found that c-Rel represents the most frequent RE in promoters regulated by all three PKCs. In addition, promoters of PKCδ-regulated genes were particularly enriched with REs for CREB, NF-E2, RREB, SRF, Oct-1, Evi-1, and NF-κB. Most notably, by using transcription factor-specific RNAi we were able to identify subsets of PKCδ-regulated genes modulated by c-Rel and CREB. Furthermore, PKCδ-regulated genes condensed under the c-Rel transcriptional regulation display significant functional interconnections with biological processes such as angiogenesis, inflammatory response, and cell motility. Conclusion/Significance Our study identified candidate transcription factors in the promoters of PKC regulated genes, in particular c-Rel was found as a key transcription factor in the control of PKCδ-regulated genes. The deconvolution of PKC-regulated transcriptional networks and their nodes may greatly help in the identification of PKC effectors and have significant therapeutics implications.
The Foundations of Probability with Black Swans
Graciela Chichilnisky
Journal of Probability and Statistics , 2010, DOI: 10.1155/2010/838240
Abstract: We extend the foundation of probability in samples with rare events that are potentially catastrophic, called black swans, such as natural hazards, market crashes, catastrophic climate change, and species extinction. Such events are generally treated as ‘‘outliers’’ and disregarded. We propose a new axiomatization of probability requiring equal treatment in the measurement of rare and frequent events—the Swan Axiom—and characterize the subjective probabilities that the axioms imply: these are neither finitely additive nor countably additive but a combination of both. They exclude countably additive probabilities as in De Groot (1970) and Arrow (1971) and are a strict subset of Savage (1954) probabilities that are finitely additive measures. Our subjective probabilities are standard distributions when the sample has no black swans. The finitely additive part assigns however more weight to rare events than do standard distributions and in that sense explains the persistent observation of ‘‘power laws’’ and ‘‘heavy tails’’ that eludes classic theory. The axioms extend earlier work by Chichilnisky (1996, 2000, 2002, 2009) to encompass the foundation of subjective probability and axiomatic treatments of subjective probability by Villegas (1964), De Groot (1963), Dubins and Savage (1965), Dubins (1975) Purves and Sudderth (1976) and of choice under uncertainty by Arrow (1971). 1. Introduction Black swans are rare events with important consequences, such as market crashes, natural hazards, global warming, and major episodes of extinction. This article is about the foundations of probability when catastrophic events are at stake. It provides a new axiomatic foundation for probability requiring sensitivity both to rare and frequent events. The study culminates in Theorem 6.1, that proves existence and representation of a probability satisfying three axioms. The last of these axioms requires sensitivity to rare events, a property that is desirable but not respected by standard probabilities. The article shows the connection between those axioms and the Axiom of Choice at the foundation of Mathematics. It defines a new type of probabilities that coincide with standard distributions when the sample is populated only by relatively frequent events. Generally, however, they are a mixture of countable and finitely additive measures, assigning more weight to black swans than do normal distributions, and predicting more realistically the incidence of “outliers,” “power laws,” and “heavy tails” [1, 2]. The article refines and extends the formulation of probability in an
Quality control in the nucleus
Graciela Flores
Genome Biology , 2005, DOI: 10.1186/gb-spotlight-20050329-02
Abstract: Protein quality control systems, which fix or rid the cell of unfolded, misfolded, or improperly modified proteins, have long been known to exist both in prokaryotes and eukaryotes. But they have been found only in compartments where protein synthesis occurs: the cytoplasm, the endoplasmic reticulum, and the mitochondria, senior author Dan Gottschling, of Seattle's Fred Hutchinson Cancer Research Center, told The Scientist."It largely has been thought that as proteins are being made, they're being sampled and destroyed right during the process of folding and assembly," said Gottschling. "We wanted to find a place where proteins that become old could be recognized and degraded, and the nucleus is the only compartment in which that could potentially happen, because there's no protein synthesis there."The team conducted what they called a "virtual genetic screen." It means that we didn't do anything except read the literature," joked Gottschling.Their approach was a rather smart one, according to Christian Hirsch of the Max-Delbrück Center for Molecular Medicine, who coauthored a related preview in the same issue of Cell. "They looked at screens that had found suppressors of temperature-sensitive mutant proteins that are functional in the cell, but that are degraded because they are recognized by a quality control system," Hirsch told The Scientist. Those results pointed to a gene that was an excellent candidate for a component of a degradation system of misfolded proteins."First we looked for nuclear proteins in yeast that were temperature sensitive," said Gottschling. "Then we looked at the subset in which there had been a suppressor screened, and we looked for a common gene that was a suppressor of all these different temperature-sensitive mutations. We found one gene, and that was SAN1, which turned out to be a ubiquitin-ligase." The team later discovered that San1 only targets aberrant proteins - not wildtype ones - and that it only functions in the nucleus."The m
Transcription factor moonlights
Graciela Flores
Genome Biology , 2005, DOI: 10.1186/gb-spotlight-20050527-01
Abstract: "Here, we show for the first time that a transcription factor has a function in the DNA damage response that is completely independent of its transcriptional activity," Ze'ev Ronai, of the Burnham Institute in La Jolla, Calif., told The Scientist.For its transcriptional regulatory roles, ATF2 is activated by phosphorylation via the JNK/p38 pathway. The group found alternate phosphorylation sites at serines 490 and 498 that are generally recognized by the kinase ataxia telangiectasia mutated (ATM), which is implicated in double-strand break (DSB) repair. They found ATF2 phosphorylated at those serines localizing at irradiation-induced DSB repair foci. When they inhibited JNK/p38 or disrupted transcriptional regulatory activity of ATF2, the protein continued to show up at these DSBs and maintained proper S-phase checkpoint, indicating that its role in DNA damage response is uncoupled from its transcriptional activities.Using antibodies to ATF2 and RNA interference, Ronai and colleagues studied ATF2 in cultured mammalian cells subjected to ionizing radiation and in ATF2 mutant mice. The results showed that ATF2 is phosphorylated by ATM and that ATF2 plays an important role in the double-strand break repair process.In the last decade, evidence supporting the idea that certain proteins - so-called moonlighting proteins - can perform multiple jobs has mounted. The novelty of this paper is that it demonstrates that the two functions of ATF2 are uncoupled."ATF2 doesn't need its transcriptional activity to participate in the DNA damage response," said Dan Mercola at the Sidney Kimmel Cancer Center, San Diego, Calif., who was not involved in the study. "The authors show that a nontranscriptionally active form appears to be aggregating at DNA damage sites. The studies are very beautiful, and the data are very convincing."William Bonner at the National Cancer Institute's Center for Cancer Research, who did not participate in the research, said the paper was an interesting devel
MicroRNAs linked to cancer
Graciela Flores
Genome Biology , 2005, DOI: 10.1186/gb-spotlight-20050610-01
Abstract: Todd Golub at the Dana-Farber Cancer Institute and the Broad Institute of the Massachusetts Institute of Technology and Harvard and coauthors report that they could accurately classify human cancers with an novel assay that they say could become a powerful diagnostic tool. In a second paper, the groups of Gregory Hannon at Cold Spring Harbor and Scott Hammond at the University of North Carolina describe elevated levels of miRNAs in human lymphoma samples and cell lines. Experimental overexpression of those miRNAs caused cancer in a mouse model.The highly conserved miRNAs and their link to cancer has been in people's minds for many years, Paul Meltzer at the National Human Genome Research Institute told The Scientist. "Previous works have reported changes in miRNA expression associated with cancer, but this new constellation of papers provides very strong support for that idea. Golub's paper provides a very nice database in terms of the pattern of expression of the 217 annotated miRNAs in human cancers," said Meltzer, who wrote an accompanying News and Views commentary.A third paper by Joshua Mendell at The Johns Hopkins University School of Medicine and colleagues unravels the connection between miRNA and c-Myc, a transcription regulator known to be overexpressed in human cancers. Most importantly, they identify an elusive miRNA target.To study miRNAs expression, Golub and his colleagues developed a bead-based flow cytometric profiling assay that first amplifies the miRNAs in the cell and then captures them on fluorescently labeled beads. "This method enabled us to measure the expression of a great number of miRNAs from samples including several human cancers," Golub told The Scientist. "We found a highly informative pattern of miRNA expression that varied across tumor types and reflected the developmental lineage as well as the differentiation state of the tumors. The patterns of which miRNAs are turned on and off in a cell can be diagnostic for whether that cell i
Zinc finger nucleases correct genes
Graciela Flores
Genome Biology , 2005, DOI: 10.1186/gb-spotlight-20050404-01
Abstract: Michael Holmes and his colleagues at Richmond, Ca. - based Sangamo Biosciences corrected a defective version of the IL2R-gamma receptor gene, which is mutant in children with X-linked severe combined immunodeficiency. Sangamo had used zinc-finger proteins before in several instances, among them, to inhibit specific single gene expression in vivo."With our technology platform here at Sangamo, we can basically engineer zinc-finger proteins to recognize virtually any sequence," Holmes, the senior author of the study, told The Scientist. "Here, we show that gene correction can be done at a specific site of the genome, where a mutation occurs, and at high frequency."The authors report that more than 18% of the cells were correctly modified without selection, of which 7% acquired the desired modification in both chromosomes."This is the highest level of gene targeting I've seen," said Dana Carroll of the University of Utah, who described the paper as "terrific." Recent estimates have found an absolute rate of gene targeting in human cells of 3 to 5%.According to Carroll, the key for the researchers' success was their ability to introduce a break in the desired gene. "Normally, gene targeting is done by isolating and manipulating a segment of the gene in the laboratory, and then putting it back into cells, hoping that homologous recombination will lead to the replacement of the endogenous gene with this manipulated gene," explained Carroll. "The problem is that the frequency of that sort of gene targeting is typically quite low - about one in a million cells gets the targeting event."Sangamo's strategy built upon two fundamental observations previously made by other researchers: that a double-strand break stimulates homologous recombination at a target and that such breaks can be made with designed zinc-finger nucleases.Although other researchers had been successful at targeting specific sequences, no one had designed the zinc-finger protein nucleases from scratch, Sriniva
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