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BIOSURFACTANT AS A PESTICIDE CLEANING AGENT IN LEAFY VEGETABLES PRODUCED BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE ECOSYSTEM
GOVINDAMMAL M,PARTHASARATHI R
Golden Research Thoughts , 2013, DOI: 10.9780/22315063
Abstract: In this present investigation, biosurfactant was produced by Pseudomonas fluorescens MFS03 isolated from mangrove forest soil using groundnut oil cake as substrate. Characterization of the biosurfactant revealed that, it is a glycolipid with chemical composition of carbohydrate (0.868 mg/ml) and lipid (1.912 mg/ml). Based on the TLC analysis, carbohydrate and lipid with an Rf value of 0.72 were separated and it was partially identified as rhamnolipid. In FT-IR spectral analysis, important absorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure is identical to those of rhamnolipids. Biosurfactant was used for the cleanup of insecticide residues in the spinach varieties Amaranthus tricolor. Lgenerally called as Arakkeerai or Killukeerai. The experiment was carried out under in-vitro conditions. Among the different washing solution (Salt, vinegar KMnO4 and biosurfactant) used for the reduction of pesticide residues, biosurfactant solution shows higher reduction. However the amount of biosurfactant and the contact times shows significant role in reducing cypermethrin residues in spinach. Cypermethrin solution with 100ppm concentration was reduced to below 2 ppm, which is safe level using 25 ppm of biosurfactant solution for 5 minutes. The increase in amount of biosurfactant concentration shows decrease in contact time in reducing cypermethrin residues. From this study we concluded that production of biosurfactant using groundnut oil cake is economically low-cost medium and eco-friendly and it can be used as an effective agent to clean up pesticide residues in vegetables and leafy vegetables.
BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL.
GOVINDAMMAL M,PARTHASARATHI R
Indian Streams Research Journal , 2013,
Abstract: In the present investigation, Pineapple juice has been tested for its suitability for biosurfactant production using Pseudomonas fluorescens MFS03 isolated from the crude oil enriched mangrove soil. The strain recorded maximum biosurfactant production (9.43 g/l) when pineapple juice was used as a medium. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, CTAB, lipase activity and emulsification index. The isolate shows lowest surface tension of 25.4 mN/m, an interfacial tension of 0.98 mN/m and a CMC of 34 mg/l. The active compound was extracted with ethyl acetate. The extract was fractionated by TLC to isolate the pure compound. Based on the TLC, chromatogram the biosurfactant was identified as glycolipid type. FT-IR spectrum revealed that the important adsorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure of rhamnolipid. The results demonstrated that the Pineapple juice can be a suitable medium for the biosurfactant production, which can improve the process economical.
INVESTIGATION ON ANTIMICROBIAL ACTIVITY OF BIOSURFACTANT PRODUCED BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE ECOSYSTEM
Govindammal M,Parthasarathi R
International Research Journal of Pharmacy , 2013,
Abstract: The aim of this present study is to investigate the antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas fluorescens MFS03 isolated from mangrove forest soil using groundnut oil cake as substrate. The biosurfactant was extracted with an equal amount of ethyl acetate and the concentrated extract was subjected to FT-IR analysis. The important adsorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1indicate the chemical structure of rhamnolipid. The rhamnolipid biosurfactant was investigated for the potential antimicrobial activity by using disc-diffusion method against Gram positive bacteria (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Methicillin resistance S. aureus) Gram negative bacteria (Escherichia coli, Salmonella typhimurium) and a yeast (Candida albicans). The biosurfactant showed distinct antibacterial activity towards tested bacteria and shows an antifungal activity against yeast. The biosurfactant with different concentration was performed for the evaluation of antimicrobial activity. Maximum antimicrobial activity of the biosurfactant (50μl) was observed in S. aureus (23 mm) and it was found that the biosurfactant activity was dependent on the concentration. So it could be used as a therapeutic agent in pharmaceutical application.
Study of Duct Characteristics Deduced from Low Latitude Ground Observations of Day-Time Whistler at Jammu  [PDF]
M. Altaf, M. M. Ahmad
International Journal of Astronomy and Astrophysics (IJAA) , 2013, DOI: 10.4236/ijaa.2013.33032
Abstract:

Propagation characteristics of low latitude whistler duct characteristics have been investigated based on day-time measurements at Jammu. The morphogical characteristics of low latitude whistlers are discussed and compared with characteristics of middle and high latitude whistlers. The Max. electron density (Nm) at the height of the ionosphere obtained from whistler dispersion comes out to be higher than that of the background which is in accordance with the characteristics of whistler duct. The equivalent width is found to be close to the satellite observations and the characteristics of whistler duct in low latitude ionosphere are similar to those in middle and high latitude ionosphere. The width of ducts estimated from the diffuseness of the whistler track observed during magnetic storm is found to lie in the range of 50 - 200 Km.

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology  [PDF]
Magdy M. M. Elnashar
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2010, DOI: 10.4236/jbnb.2010.11008
Abstract: Immobilized molecules using biomaterials and nanobiotechnology is a very interesting topic that touching almost all aspects of our life. It uses the sciences of biology, chemistry, physics, materials engineering and computer science to develop instruments and products that are at the cutting edge of some of today’s most promising scientific frontiers. In this review article, the author based on his experience in this arena has tried to focus on some of the supports for im-mobilization; the most important molecules to be immobilized such as DNA, cells, enzymes, metals, polysaccharides, etc and their applications in medicine, food, drug, water treatment, energy and even in aerospace. He specified a special section on what is new in the arena of supports and technologies used in enzyme immobilization and finally a recommendation by the author for future work with a special attention to up-to-date references.
Using of the generalized special relativity (GSR) in estimating the neutrino masses to explain the conversion of electron neutrinos  [PDF]
M. H. M. Hilo
Natural Science (NS) , 2011, DOI: 10.4236/ns.2011.34044
Abstract: In this work the Generalized Special Relativity (GSR) is utilized to estimate masses of some elementary particles such as, neutrinos. These results are found to be in conformity with experimental and theoretical data. The results obtained may explain some physical phenomena, such as, conversion of neutrinos from type to type when solar neutrino reaches the Earth.
Postischemic Lower Extremity Wounds Treated with and without Negative Pressure Dressing  [PDF]
Naz?mmü?
Surgical Science (SS) , 2012, DOI: 10.4236/ss.2012.37075
Abstract: Background: Vacuum-assisted dressing is a noninvasive closure system of the wound, which makes localized and controlled negative pressure. Its mechanical tension reduces edema, stimulates granulation tissue formation and angiogenesis, and prepares the wound bed for closure. In this study, a patient has been presented, who suffered from serious lower extremity wounds due to arterial emboli, one of her wounds has been treated with vacuum-assisted dressing and the other with conventional dressing to evaluate the efficacy of vacuum-assisted dressing in acute ischemic wounds. Methods: A 65-year-old female patient was admitted to the emergency clinic, due to complaint of severe pain in her right lower extremity which suggested an acute arterial occlusion. She immediately underwent an embolectomy operation; however, a few days later, severe ischemia on the leg and foot became appearant. All of the necrosis was sharply debrided under sterile conditions in the operating room, and then lower leg wound was covered with vacuum-assisted dressing, also calcaneal wound was dressed with silver sulphadiazine. Results: Eighteen days after the first dressing with negative pressure, distal leg wound became ready for closure, whereas, enough granulation tissue over the calcaneal area developed merely thirty-four days later. Calcaneal wound closed spontaneously within fifty-three days. Conclusions: When dealing with this experience, using the negative pressure dressing in patient with severe lower limb wounds following arterial emboli, accelerates wound healing by means of developing the granulation tissue, and rapidly prepares the wound for closure, so it may reduce the risk of amputation.
Laplace Transform Method for Unsteady Thin Film Flow of a Second Grade Fluid through a Porous Medium  [PDF]
M. Ali, M. Awais
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.53017
Abstract:

In this article, we have effectively used the Numerical Inversion of Laplace transform to study the time-dependent thin film flow of a second grade fluid flowing down an inclined plane through a porous medium. The solution to the governing equation is obtained by using the standard Laplace transform. However, to transform the obtained solutions from Laplace space back to the original space, we have used the Numerical Inversion of Laplace transform. Graphical results have been presented to show the effects of different parameters involved and to show how the fluid flow evolves with time.

An Integral Collocation Approach Based on Legendre Polynomials for Solving Riccati, Logistic and Delay Differential Equations  [PDF]
M. M. Khader, A. M. S. Mahdy, M. M. Shehata
Applied Mathematics (AM) , 2014, DOI: 10.4236/am.2014.515228
Abstract:

In this paper, we propose and analyze some schemes of the integral collocation formulation based on Legendre polynomials. We implement these formulae to solve numerically Riccati, Logistic and delay differential equations with variable coefficients. The properties of the Legendre polynomials are used to reduce the proposed problems to the solution of non-linear system of algebraic equations using Newton iteration method. We give numerical results to satisfy the accuracy and the applicability of the proposed schemes.

ДОСЛ ДЖЕННЯ АНАЛ ТИЧНОГО МЕТОДУ ОЦ НЮВАННЯ ЙМОВ РНОСТ ПОТЕНЦ ЙНО КОНФЛ КТНИХ СИТУАЦ Й В ПОВ ТРЯНОМУ РУС The study of the analytical method of evaluating the probability of potential conflict situations in air traffic Исследование аналитического метода оценки вероятности потенциально конфликтных ситуаций в воздушном движении
В.M. Василь?в
Proceedings of National Aviation University , 2007,
Abstract: Розглянуто застосування запропонованого ран ше методу оц нювання ймов рност потенц йних конфл кт в для системи керування пов тряним рухом. Досл джено властивост методу, наведено проанал зовано результати комп’ютерного моделювання. Application of the method of potential conflicts probability evaluation offered before for air traffic control system is considered. The properties of the method are investigated, results of computer modeling are represented and analyzed. Рассмотрено применение предложенного ранее метода оценки вероятности потенциальных конфликтов для системы управления воздушным движением. Исследованы свойства метода, приведены и проанализированы результаты компьютерного моделирования.
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