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Search Results: 1 - 10 of 1119 matches for " Gordana Joksi "
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Simple Fluorescent Micronucleus Assay-combination of Hg-banding Technique and In situ Hybridisation
Gordana Joksi,Angelina Petrovi?-Novak
Journal of Medical Sciences , 2001,
Abstract: Recent development in cytogenetic and molecular techniques, particularly combination of cytochalasin B method and fluorescence In situ hybridisation (FISH) now allow sensitive identification of clastogenic versus aneugenic events. To simplify the fluorescence micronucleus assay a technique based on combination of hypotonic banding and In situ hybridisation with pancentromeric probe (SO-αAllCen) is described. This work demonstrates more comfortable, easier and faster way to perform fluorescent micronucleus test
Positive correlation between micronuclei and necrosis of lymphocytes in medical personnel occupationally exposed to ionizing radiation
Petrovi? Sandra,Leskovac Andreja,JoksiGordana
Archive of Oncology , 2005, DOI: 10.2298/aoo0502065p
Abstract: BACKGROUND: Current radiation protection standards are based on premise that any radiation dose may result in detrimental health effects. The aim of this study was to evaluate extent of the DNA damages (measured by induction of micronuclei) and interphase cell death in circulating lymphocytes of medical personnel exposed to ionizing radiation. METHODS: Baseline micronuclei were assessed using the cytokinesis-block micronucleus test. Cytotoxicity was analyzed by flow cytometry for human white blood cells to identify cells that displayed apoptosis-associated DNA condensation. Necrotic cells were analyzed simultaneously. All parameters were compared with corresponding controls. RESULTS: A statistically significant difference (t = 4.54, p = 0.002) was found between exposed and control group in the yield of baseline micronuclei. The level of baseline micronuclei correlated positively with necrosis of leucocytes (r=0.09, p=0.68 in exposed group, r=0.02, p=0.97 in controls). An inverse correlation between baseline micronuclei and apoptosis was noted in both groups of examinees (r = -0.26, p = 0.27 in exposed group, r = -0.09, p=0.80 in controls). The data obtained also suggested an inverse correlation between necrosis and apoptosis (r = -0.37, p = 0.11 in exposed group, r = -0.89, p = 0.001 in controls). CONCLUSION: Flow cytometry being a rapid, fast, and accurate method is strongly recommended in evaluation of radiation injuries. The integration of apoptosis and necrosis into micronucleus assay could be very important in the assessment of cumulative effects of ionizing radiation in occupationally exposed medical personnel.
Incidence of micronuclei in pregnant women and cord blood samples before and after the bombing of Serbia
Stankovi? Miroslava,JoksiGordana,Gu?-??eki? Marija P.
Archive of Oncology , 2004, DOI: 10.2298/aoo0404200s
Abstract: BACKGROUND: This study provides the data regarding monitoring of population using CB-micronuclei assay in the period 1995-2001 in Serbia. The target groups consisted of 45 pregnant women of mean age 34.3(6.56) years, unaware of being exposed to chemicals drugs or other substances and undergoing cordocentesis. The incidence of micronuclei (MN) in peripheral blood lymphocytes and in fetal cord blood lymphocytes was analyzed. METHODS: The study was carried out on cultures of PHA-stimulated blood lymphocytes. Three drops of blood samples ware added into 5ml RPMI-1640 (Gibco) medium supplemented with 15% of calf serum and PHA (Gibco, 2.5μg/ml). For micronuclei preparation the cytokinesis block method was used (Fenech et al., 1993). RESULTS: The results of the study showed that in the year 1995, the incidence of micronuclei in pregnant women was 9.61(3.26) per 1000 binucleated (BN) cells, and 3.74(1.60) in cord blood samples per 1000 BN cells, respectively. In 2000 the incidence of micronuclei in study group was 28.26(7.87) per 1000 BN cells, and in cord blood samples 22.22(5.63) per 1000 BN cells. One year later (2001) the incidence of micronuclei in pregnant woman slightly decreased and reached the value of 26.98(4.50), while in cord blood it slightly increased up to 26.58(6.85) per 1000 BN cells. CONCLUSION: The monitoring data obtained in this study have shown significantly increase of micronuclei (2- to 3-fold) in study groups in 2000 and 2001.
Radioprotective properties of nutraceutical Gonebazol: In vivo study
JoksiGordana,Ili? Nevenka,Spasojevi?-Ti?ma Vera
Archive of Oncology , 2006, DOI: 10.2298/aoo0602015j
Abstract: BACKGROUND: In vitro investigation of radioprotective properties of novel nutraceutical Gonebazol (Biofarm, Belgrade) has displayed its remarkable potential to reduce chromosomal aberrations and micronuclei induced by x rays. The goal of this study was to evaluate its protective properties in vivo. For this purpose, a group of medical staff performing invasive radiological diagnostics, that was identified to carry dicentrics in their lymphocytes, was selected for further monitoring. They consumed this nutraceutical for 3 weeks, 2 x 10 g per day (2 x 2 teaspoons dissolved in a glass of water). METHODS: Hematological parameters, chromosomal aberrations and micronucleus frequency were examined at the beginning of monitoring, on day 10 and 21 of the treatment. RESULTS: In the course of three weeks the incidence of chromosomal aberrations and micronuclei was significantly reduced (50%-80%), absolute number of granulocytes was lowered whereas the number of monocytes significantly was enhanced. This study has revealed that nutraceutic posses immunomodulatory properties seen as an improving monocyte-macrophage activity, which inversely correlates with incidence of lymphocyte micronuclei (r=0.75, p<0.05). CONCLUSION: Observed finding could be of particular importance in reducing cumulative effects of ionizing radiation in radiosensitive tissues and in preventing adverse health effects. No side effects were evidenced. Mechanisms of its immunomodulatory effects should be further examined.
Inhibition of Aldose Reductase by Gentiana lutea Extracts
Chandrasekhar Akileshwari,Puppala Muthenna,Branislav Nastasijevi ,Gordana Joksi ,J. Mark Petrash,Geereddy Bhanuprakash Reddy
Experimental Diabetes Research , 2012, DOI: 10.1155/2012/147965
Abstract: Accumulation of intracellular sorbitol due to increased aldose reductase (ALR2) activity has been implicated in the development of various secondary complications of diabetes. Thus, ALR2 inhibition could be an effective strategy in the prevention or delay of certain diabetic complications. Gentiana lutea grows naturally in the central and southern areas of Europe. Its roots are commonly consumed as a beverage in some European countries and are also known to have medicinal properties. The water, ethanol, methanol, and ether extracts of the roots of G. lutea were subjected to in vitro bioassay to evaluate their inhibitory activity on the ALR2. While the ether and methanol extracts showed greater inhibitory activities against both rat lens and human ALR2, the water and ethanol extracts showed moderate inhibitory activities. Moreover, the ether and methanol extracts of G. lutea roots significantly and dose-dependently inhibited sorbitol accumulation in human erythrocytes under high glucose conditions. Molecular docking studies with the constituents commonly present in the roots of G. lutea indicate that a secoiridoid glycoside, amarogentin, may be a potential inhibitor of ALR2. This is the first paper that shows G. lutea extracts exhibit inhibitory activity towards ALR2 and these results suggest that Gentiana or its constituents might be useful to prevent or treat diabetic complications.
Randomized, placebo-controlled, double-blind trial of Swedish snus for smoking reduction and cessation
Gordana Joksi, Vera Spasojevi?-Ti?ma, Ruza Anti?, Robert Nilsson, Lars E Rutqvist
Harm Reduction Journal , 2011, DOI: 10.1186/1477-7517-8-25
Abstract: We conducted a randomized, double-blind, placebo-controlled, clinical trial aimed at assessing the efficacy of snus to help adult cigarette smokers in Serbia to substantially reduce, and, eventually, completely stop smoking. The study enrolled 319 healthy smokers aged 20-65 years at two occupational health centers in Belgrade, Serbia. Most of them (81%) expressed an interest to quit rather than just reduce their smoking. Study products were used ad libitum throughout the 48-week study period. The main study objective during the first 24 weeks was smoking reduction. The primary end-point was defined as a biologically verified reduction of ≥ 50% in the average number of smoked cigarettes per day during week 21-24 compared to baseline. During week 25-48 participants were actively instructed to stop smoking completely. Outcome measures of biologically verified, complete smoking cessation included 1-week point prevalence rates at clinical visits after 12, 24, 36, and 48 weeks, as well as 4-, 12- and 24-week continued cessation rates at the week 36 and 48 visits.At the week 24 visit, the proportion of participants who achieved the protocol definition of a ≥ 50% smoking reduction was similar in the two treatment groups. However, the proportion that reported more extreme reductions (≥ 75%) was statistically significantly higher in the snus group than in the placebo group (p < 0.01). The results for biologically verified complete cessation suggested that participants in the snus group were more likely to quit smoking completely than the controls; the odds ratio (snus versus placebo) for the protocol estimates of cessation varied between 1.9 to 3.4, but these ratios were of borderline significance with p-values ranging from 0.04-0.10. Snus was well tolerated and only 2/158 (1.3%) participants in the snus group discontinued treatment due to an adverse event (in both cases unrelated to snus).Swedish snus could promote smoking cessation among smokers in Serbia, that is, in a cul
Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts
Rushendhiran Kesavan, Uma Rani Potunuru, Branislav Nastasijevi?, Avaneesh T, Gordana Joksi, Madhulika Dixit
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061393
Abstract: Gentiana lutea belonging to the Gentianaceae family of flowering plants are routinely used in traditional Serbian medicine for their beneficial gastro-intestinal and anti-inflammatory properties. The aim of the study was to determine whether aqueous root extracts of Gentiana lutea consisting of gentiopicroside, gentisin, bellidifolin-8-O-glucoside, demethylbellidifolin-8-O-glucoside, isovitexin, swertiamarin and amarogentin prevents proliferation of aortic smooth muscle cells in response to PDGF-BB. Cell proliferation and cell cycle analysis were performed based on alamar blue assay and propidium iodide labeling respectively. In primary cultures of rat aortic smooth muscle cells (RASMCs), PDGF-BB (20 ng/ml) induced a two-fold increase in cell proliferation which was significantly blocked by the root extract (1 mg/ml). The root extract also prevented the S-phase entry of synchronized cells in response to PDGF. Furthermore, PDGF-BB induced ERK1/2 activation and consequent increase in cellular nitric oxide (NO) levels were also blocked by the extract. These effects of extract were due to blockade of PDGF-BB induced expression of iNOS, cyclin D1 and proliferating cell nuclear antigen (PCNA). Docking analysis of the extract components on MEK1, the upstream ERK1/2 activating kinase using AutoDock4, indicated a likely binding of isovitexin to the inhibitor binding site of MEK1. Experiments performed with purified isovitexin demonstrated that it successfully blocks PDGF-induced ERK1/2 activation and proliferation of RASMCs in cell culture. Thus, Gentiana lutea can provide novel candidates for prevention and treatment of atherosclerosis.
Inhibition of Aldose Reductase by Gentiana lutea Extracts
Chandrasekhar Akileshwari,Puppala Muthenna,Branislav Nastasijevi?,Gordana Joksi,J. Mark Petrash,Geereddy Bhanuprakash Reddy
Journal of Diabetes Research , 2012, DOI: 10.1155/2012/147965
Abstract: Accumulation of intracellular sorbitol due to increased aldose reductase (ALR2) activity has been implicated in the development of various secondary complications of diabetes. Thus, ALR2 inhibition could be an effective strategy in the prevention or delay of certain diabetic complications. Gentiana lutea grows naturally in the central and southern areas of Europe. Its roots are commonly consumed as a beverage in some European countries and are also known to have medicinal properties. The water, ethanol, methanol, and ether extracts of the roots of G. lutea were subjected to in vitro bioassay to evaluate their inhibitory activity on the ALR2. While the ether and methanol extracts showed greater inhibitory activities against both rat lens and human ALR2, the water and ethanol extracts showed moderate inhibitory activities. Moreover, the ether and methanol extracts of G. lutea roots significantly and dose-dependently inhibited sorbitol accumulation in human erythrocytes under high glucose conditions. Molecular docking studies with the constituents commonly present in the roots of G. lutea indicate that a secoiridoid glycoside, amarogentin, may be a potential inhibitor of ALR2. This is the first paper that shows G. lutea extracts exhibit inhibitory activity towards ALR2 and these results suggest that Gentiana or its constituents might be useful to prevent or treat diabetic complications. 1. Introduction According to the latest WHO estimates, currently approximately 200 million people all over the world are suffering from diabetes. This may increase to at least 350 million by the year 2025, which could have a severe impact on human health [1]. Prolonged exposure to chronic hyperglycemia in diabetes can lead to various complications affecting the cardiovascular, renal, neurological, and visual systems [2]. Although mechanisms leading to diabetic complications are not completely understood, many biochemical pathways associated with hyperglycemia have been implicated [2]. Among these, the polyol pathway has been extensively studied [3]. Aldose reductase (ALR2; EC: 1.1.1.21) belongs to aldo-keto reductases (AKR) super family. It is the first and rate-limiting enzyme in the polyol pathway where it reduces glucose to sorbitol utilizing NADPH as a cofactor. Subsequently, sorbitol dehydrogenase catalyzes the conversion of sorbitol to fructose, thus constituting the polyol pathway [3]. Accumulation of sorbitol leads to osmotic swelling, changes in membrane permeability, and also oxidative stress culminating in tissue injury [4]. Experimental animal models suggest
Elements of spatial data quality as information technology support for sustainable development planning
Joksi? Du?an,Bajat Branislav
Spatium , 2004, DOI: 10.2298/spat0411077j
Abstract: We are witnessing nowadays that the last decade of the past century, as well as the first years of the present one, have brought technology expansion with respect to spatial data gathering and processing which makes a physical basis for management of spatial development. This has resulted in enlargement of the spatial data market. New technologies, presented in computer applications, have greatly expanded the number of users of these products. The philosophy of spatial data collecting has changed; analogue maps and plans printed on paper have been replaced by digital data bases which enable their presentation in a way that is the best for a particular user. Further, digital spatial data bases provide the possibility of their further upgrading by users. The two aspects, with respect to circumstances mentioned above, are very important in the process of data bases production and distribution. Firstly, the users of these data bases should be the ones who decide which of the available bases could satisfy their requirements, or in other words, what is the data quality level necessary for a certain application. On the other hand, the visualization of digital data bases could often mislead, since review of data bases could present data with better accuracy then the actual one. Thus, certain methods that would point to a quality of the selected data in the process of their analysis should be available to users. Specific, already adopted international standards, or specially developed procedures and methodologies, so called de facto standards, could be used in this data processing, enabling the estimation of these data quality. The development of Open GIS concept requires the adoption of widely accepted standards for spatial data quality. It is recommended that ISO standards should be accepted, firstly TC211 standards which are related to geographic information and geomatics. The realization of projects on ISO standards should be finished by 2006, so, all participants of these data bases should be both familiar with this project and ready to adapt to the given solutions. The basic components defining quality of data bases are explained by this work, and the results of the standardization regarding the procedures and methodology of their quality assessment, obtained so far, are also presented.
Probability maps as a measure of reliability for indivisibility analysis
Joksi? Du?an,Bajat Branislav
Spatium , 2005, DOI: 10.2298/spat0512022j
Abstract: Digital terrain models (DTMs) represent segments of spatial data bases related to presentation of terrain features and landforms. Square grid elevation models (DEMs) have emerged as the most widely used structure during the past decade because of their simplicity and simple computer implementation. They have become an important segment of Topographic Information Systems (TIS), storing natural and artificial landscape in forms of digital models. This kind of a data structure is especially suitable for morph metric terrain evaluation and analysis, which is very important in environmental and urban planning and Earth surface modeling applications. One of the most often used functionalities of Geographical information systems software packages is indivisibility or view shed analysis of terrain. Indivisibility determination from analog topographic maps may be very exhausting, because of the large number of profiles that have to be extracted and compared. Terrain representation in form of the DEMs databases facilitates this task. This paper describes simple algorithm for terrain view shed analysis by using DEMs database structures, taking into consideration the influence of uncertainties of such data to the results obtained thus far. The concept of probability maps is introduced as a mean for evaluation of results, and is presented as thematic display.
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