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Search Results: 1 - 10 of 34952 matches for " Giuseppina Pace Pereira Lima "
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A??o do BAP na regenera??o in vitro de Blc Owen Holmes Ponkan x Brassavola digbiana no 2
Giatti, Luciana;Lima, Giuseppina Pace Pereira;
Ciência e Agrotecnologia , 2007, DOI: 10.1590/S1413-70542007000500001
Abstract: cattleya is considered the most beautiful and searched among all orchids. this fact creates the need of developing more effective propagation techniques to become available a greater number of individuals in reduced periods of time and physical space, besides maintenance of uniformity and genotipic identity to meet the needs of the market. the objective of this work was to evaluate the regeneration of the cultivated shoot meristematic in vitro of the hybrid blc owen holmes ponkan x brassavola digbiana no 2 in the different bap (6-benzylaminopurine) concentrations. the explants were inoculated in the liquid medium with or without growth regulators in the following combinations: t1: ms liquid + coconut water; t2: ms + coconut water + 0.1 mg l-1 naa + 0.2 mg l-1 bap; t3: ms + coconut water + 0.1 mg l-1 naa + 0.5 mg l-1 bap and t4: ms + coconut water + 0.1 mg l-1 naa + 1.0 mg l-1 bap. subcultivatings and evaluations (percentage of survival, contamination, medium color, oxidation and regeneration) were made at 18, 32, 62, 93, 123, 153, and 184 days. at 93 days the first signs of regeneration were observed. bap (1.0 mg l-1) enhanced good results with the formation of 132 sprouts in the 184 days, concluded that the utilization of bap improved morphogenic response with a higher number of shoots.
Cádmio e a atividade de peroxidase durante a germina??o de sementes de feijoeiro
Rossi, Celso;Lima, Giuseppina Pace Pereira;
Scientia Agricola , 2001, DOI: 10.1590/S0103-90162001000100030
Abstract: seeds of common bean seeds cv. carioca were germinated in the dark in different concentrations of cadmium (1, 2, 5 and 10 mmol l-1) and collected after 24, 48, 72 and 96 hours with the objective of verifying if theperoxidase activity could be used as a biochemical marker of stress induced by cadmium. alterations in the peroxidase activity (ec were oberved in the cotyledons as well as in roots, as a function of the exhibition time of seeds in different cadmium concentrations. this enzyme can be used as a stress marker caused by cadmium during bean seed germination as an indicative of possible injure of the seeds by this metal.
Cádmio e a atividade de peroxidase durante a germina o de sementes de feijoeiro
Rossi Celso,Lima Giuseppina Pace Pereira
Scientia Agricola , 2001,
Abstract: Sementes de feijoeiro cv. Carioca, foram germinadas no escuro em diferentes concentra es de cádmio (1, 2, 5 e 10 mimol L-1) e coletadas após 24, 48 , 72 e 96 horas com o objetivo de verificar se a atividade da peroxidase, poderia ser utilizada como marcador bioquímico de estresse promovido pelo cádmio. Observaram-se altera es na atividade específica da peroxidase (EC tanto nos cotilédones como nas raízes, em fun o do tempo de exposi o das sementes nas diferentes concentra es de cádmio. A enzima peroxidase (EC pode ser usada como um indicador de estresse causado pelo cádmio durante a germina o de sementes de feij o, indicando possíveis injúrias causadas nas sementes pelo metal.
Sanitizers Effect in Mango Pulp and Peel Antioxidant Compounds  [PDF]
Kamila de Almeida Monaco, Sergio Marques Costa, Maíra Rodrigues Uliana, Giuseppina Pace Pereira Lima
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.510103

Effects of ozonated water as sanitizer method on mango was studied on total phenolics, flavonoids, carotenoids and vitamin C of pulp or peel. Mango cultivar “Palmer” was harvested and subjected to sanitization treatments by immersion in water, chlorinated water (10 minutes sodium hypochlorite 100 mg·L-1) or ozonated water for 10 and 20 minutes. After the sanitization process, the mangoes were stored at 15°C ± 1°C and 85% ± 5% RH for seven days, followed by 4 days of storage at room temperature (simulating the trading period), totaling 11 days after harvested. Mangoes pulp sanitized with ozonated water for 20 minutes showed the highest values of TA, total soluble carbohydrates, vitamin C, carotenoids and flavonoid content. These data suggest that the use of ozonated water may contribute to induce increase antioxidants compounds.

Antioxidant Properties and Chemical Composition Relationship of Europeans and Brazilians Propolis  [PDF]
Sabrina Fabris, Mariangela Bertelle, Oxana Astafyeva, Elena Gregoris, Roberta Zangrando, Andrea Gambaro, Giuseppina Pace Pereira Lima, Roberto Stevanato
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.41006
Abstract: The antioxidant activity of ethanol extracts of propolis, bee glue, of various climate and orographic characteristics, collected from Italy, Brazil and Russia, was evaluated measuring their inhibitory action on peroxidation of linoleic acid, radical scavenging ability towards 2,2’-diphenyl-1-picrylhydrazyl, total phenolic content and reducing capacity by enzymatic and Folin method respectively. Propolis samples were chemically characterized by HPLC-MS/MS in order to find a possible correlation between antioxidant activity and polyphenols composition and quantification. The results obtained indicate that Italian and Russian propolis samples have similar polyphenolic composition and, as a consequence, almost similar antioxidant activity, while Brazilian propolis evidence lower polyphenolic and antioxidant characteristics. Climate and orography reasons of these differences are also suggested.
Polyphenols in Fruits and Vegetables and Its Effect on Human Health  [PDF]
Giuseppina Pace Pereira Lima, Fabio Vianello, Camila Renata Corrêa, Renê Arnoux da Silva Campos, Milena Galhardo Borguini
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.511117

Polyphenols represent a group of chemical substances common in plants, structurally characterized by the presence of one or more phenol units. Polyphenols are the most abundant antioxidants in human diets and the largest and best studied class of polyphenols is flavonoids, which include several thousand compounds. Numerous studies confirm that they exert a protective action on human health and are key components of a healthy and balanced diet. Epidemiological studies correlate flavonoid intake with a reduced incidence of chronic diseases, such as cardiovascular disease, diabetes and cancer. The involvement of reactive oxygen species (ROS) in the etiology of these degenerative conditions has suggested that phytochemicals showing antioxidant activity may contribute to the prevention of these pathologies. The present review deals with phenolic compounds in plants and reports on recent studies. Moreover, the present work includes information on the relationships between the consumption of these compounds, via feeding, and risk of disease occurrence, i.e. the effect on human health. Results obtained on herbs, essential oils, from plants grown in tropical, subtropical and temperate regions, were also reported.

Estudo das poliaminas na morfogênese in vitro de Hemerocallis sp.
Debiasi, Clayton;Fráguas, Chrystiane Borges;Lima, Giuseppina Pace Pereira;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000400015
Abstract: in tissue culture, the action of induction of the vegetal development and also oxidative control of cells and tissues are attributed to the polyamines. this study was aimed at verifing the participation of the polyamines spermidine (spd), spermine (spm) and putrescine (put) in the induction of callus differentiation and in the oxidative control at hemerocallis sp. cultivated in vitro. cell mass produced by callus in vitro were cultivated in medium ms, supplemented with spd, spm and put in different concentrations and combinations, by evaluating endogenous contents of polyamines and soluble proteins, and peroxidase activity (ec, number and height of shoots, hyperhydricity and necrosis, and formation of calluses. the results indicate that polyamines participate not only at the morphogenesis induction, but also to the oxidative standards of cells and tissues. at the end of the study put, isolated (10μm put) or with spd (10μm 10 spd + 10μm put) or spm (10μm spm + 10μm put), induced the biggest average related to number and height of differentiated shoots. already the combination of the three polyamines in the medium (10μm spd + 10μm spm + 10μm put) induced mayor parts (60%) of micro shoots in the callus. the spd and spm combination (10μm spd + 10μm spm) induced the biggest quantity of necrosis tissue (65%). it was verified that spm, applied exogenously in the medium (10 μm spm) can act as antioxidant in the tissue culture of this species.
Espermidina e espermina exogena na micropropaga??o de Aloe vera (L.) Burm
Mógor,Gilda; Pace Pereira Lima,Giuseppina; Mogor,Atila;
Revista Cubana de Plantas Medicinales , 2007,
Abstract: the lateral shoots of the aloe vera l. burm have been inoculated in culture medium. the work has been divided in two phases. in the phase i different concentrations of naa and bap were added to the ms . in the phase ii, plants proceeding of the ms were inoculated in medium containing or not spermidine and/or spermine. in the phase i the best results for mass production and shooting came out with the use of ms + 8,88 m mol l -1 bap + 5,36 m mol l -1 naa and these treatments didn′t induce the rooting. plants submitted to treatments with polyamines presented emission of roots during the phase ii, suggesting possible inductor effect when used by isolated means during the rooting. no traces of oxidation were noted in the culture medium containing the polyamines. the use of spermidine in the culture-medium promoted a greater mass increasing and the most number of shoots was obtained with the use of the combination of polyamines.
Espermidina e espermina exogena na micropropaga o de Aloe vera (L.) Burm Es permidine y espermine exógeno en el micropropagación de Aloe vera (L.) Burm Exogenous spermidine and spermine in micropropagation of Aloe vera (L.) Burm
Gilda Mógor,Giuseppina Pace Pereira Lima,Atila Mogor
Revista Cubana de Plantas Medicinales , 2007,
Abstract: ápices caulinares de Aloe vera L. Burm. foram inoculados em meios de cultura. O experimento foi dividido em duas fases. Na fase I diferentes concentra es e combina es de NAA e BAP foram adicionadas ao meio MS . Na fase II, p lantas provenientes de MS foram inoculadas em meio contendo ou n o espermidina e/ou espermina. Na fase I, os melhores resultados para produ o de massa e perfilhamento ocorreram em MS + 8,88 m mol.L -1 BAP + 5,36 m mol.L -1 NAA e n o foi observado enraizamento. Plantas submetidas aos tratamentos com poliaminas (10 mM) apresentaram emiss o de raízes na fase II, sugerindo possível efeito indutor, quando usadas isoladamente durante a rizogênese. N o foram notados indícios de oxida o no meio de cultura contendo poliaminas. O uso de espermidina no meio de cultura promoveu maior incremento de massa e o maior número de perfilhos foi obtido com o uso da combina o das poliaminas. ápices caulinares de plantas de Aloe vera L. Burm. fueron inoculados en medios de cultivo. El experimento fue dividido en dos fases. En la fase l diferentes concentraciones y combinaciones de NAA y BAP fueron adicionadas al medio MS. En la fase II, plantas provenientes del tratamiento MS fueron inoculadas en medio que contenía, o no, espermidina y/ o espermina. En la fase I, los mejores resultados para producción de masa y brotaciones ocurrieron con el uso de MS + 8,88 m mol L -1 BAP + 5,36 m mol L -1 NAA y no indujeron al enraizamiento. Las plantas sometidas a los tratamientos con espermidina o espermina (10 mM) presentaron emisión de raíces durante la fase II, sugiriendo un posible efecto inductor, cuando fueron usadas aisladamente durante la rizogénesis. No fueron notados indicios de oxidación en el medio de cultivo que contenía poliaminas. El uso de espermidina en el medio de cultivo promovió mayor incremento de masa y un mayor número de brotaciones fue obtenido con el uso de la combinación de las poliaminas. The lateral shoots of the Aloe vera L. Burm have been inoculated in culture medium. The work has been divided in two phases. In the phase I different concentrations of NAA and BAP were added to the MS . In the phase II, plants proceeding of the MS were inoculated in medium containing or not spermidine and/or spermine. In the phase I the best results for mass production and shooting came out with the use of MS + 8,88 m mol L -1 BAP + 5,36 m mol L -1 NAA and these treatments didn′t induce the rooting. Plants submitted to treatments with polyamines presented emission of roots during the phase II, suggesting possible inductor effect when used by isolated me
Export of nutrients in plants jambu under different fertilization Exporta o de nutrientes em plantas de jambu, sob diferentes aduba es
Luciana da Silva Borges,Rumy Goto,Giuseppina Pace Pereira Lima
Semina : Ciências Agrárias , 2013,
Abstract: The jambu is a broad vegetable consumption in Northern Brazil, especially in Pará, known by the jambu and other common names is native to the Amazon region has been used and cultivated for culinary and also recently in natural medicines by their chemical properties, attributed to the spilanthol compound. Knowing the amount of nutrient uptake in plants, especially at the taken, it is important to evaluate the removal of nutrients necessary for economic fertilizer recommendations. So the goal of this project was to determine the accumulation of nutrients in plants of jambu (leaf and inflorescence) under different fertilizations. The experiment was conducted at S o Manuel Experimental Farm UNESP. The statistical was arranged in the randomized block design, in a 2 x 6 factorial scheme, two sources of fertilizers (organic and mineral) and six doses of nitrogen, with four replications. We evaluated the macronutrients of accumulation N, P, K, Ca, Mg, S and micronutrients of accumulation B, Cu, Fe and Zn in leaves and inflorescence. The plants responded more jambu nutrients of translocation phosphorus (P), magnesium (Mg), sulfhur (S), boron (B), copper (Cu) and iron (Fe) in the inflorescences and phosphorus (P), calcium (Ca), manganese (Mg), sulfur (S), boron (B), copper (Cu) and iron (Fe) in leaves to organic fertilization demonstrating the effectiveness of using this source of fertilizer nutrients indicating that this was a defining characteristic in response to the accumulation of nutrients in the leaves and inflorescences jambu. Plants jambu are more responsive to fertilizer for the mineral of translocation nitrogen (N) and manganese (Mn) for both the sheet and for the inflorescences of plants jambu. O jambu é uma hortali a de largo consumo na regi o Norte do Brasil, conhecida por diferentes nomes populares, como agri o do Pará, erva maluca, bot o de ouro, é uma espécie nativa da Amaz nia, bastante utilizada na culinária regional e também em medicamentos naturais, pela suas propriedades químicas, atribuído ao composto espilantol. O conhecimento da quantidade de nutrientes acumulados nas plantas, principalmente na parte colhida, é importante para se avaliar a remo o dos nutrientes necessários para as recomenda es econ micas de aduba o. Assim o objetivo deste projeto foi determinar o acúmulo de nutrientes em plantas de jambu (folha e inflorescência) sob diferentes aduba es. O experimento foi conduzido na Fazenda Experimental S o Manuel, FCA/UNESP. O delineamento estatístico foi em blocos casualizados, com esquema fatorial (2 x 6), sendo duas fontes de adub
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