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Search Results: 1 - 10 of 181357 matches for " Gitahy Patrícia de Medeiros "
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Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis
Salles, Joana Falc?o;Gitahy, Patrícia de Medeiros;Sk?t, Leif;Baldani, José Ivo;
Brazilian Journal of Microbiology , 2000, DOI: 10.1590/S1517-83822000000300001
Abstract: the goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. the endophytic nitrogen-fixing bacteria gluconacetobacter diazotrophicus strain br11281 and herbaspirillum seropedicae strain br11335 were used as models. the cry3a gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid n-free medium. the presence of the cry gene was detected by southern-blot using an internal fragment of 1.0 kb as a probe. the production of d-endotoxin by the recombinant h. seropedicae strain was detected by dot blot while for g. diazotrophicus the western-blot technique was used. in both cases, a specific antibody raised against the b. thuringiensis toxin was applied. the d-endotoxin production showed by the g. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3a gene was fused to a nif promoter. in the case of h. seropedicae the d-endotoxin expression was not affected by the promoter (rhi) used. these results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis
Salles Joana Falc?o,Gitahy Patrícia de Medeiros,Sk?t Leif,Baldani José Ivo
Brazilian Journal of Microbiology , 2000,
Abstract: The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Western-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The delta-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the delta-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)
Gitahy, Patrícia de Medeiros;Souza, Marlene Teixeira de;Monnerat, Rose Gomes;Arrigoni, Enrico de Beni;Baldani, José Ivo;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000300028
Abstract: the control of the major sugarcane pest, diatraea saccharalis, is limited by the stem location of the caterpillar. as part of a long-term project towards the development of an alternative and efficient delivery system of cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a brazilian b. thuringiensis strain with prominent activity towards d. saccharalis. strain s76 was eleven-fold more active than the hd-1 lepidoptera-standard strain, as estimated by the lc50 of 13.06 μg/l and 143.88 μg/l, respectively. we observed bipiramidal and cuboidal crystals similar to those found in other b. thuringiensis strains with entomopathogenic activity against lepidoptera and diptera. in addition, smaller and spherical crystalline inclusions were also observed. the plasmid profile of strain s76 is similar to that of hd-1. pcr amplifications of s76 dna using cry specific primers confirmed the presence of cry1aa,cry1ab,cry1ac,cry2aa1, and cry2ab2, but not cry1ad, cry2ac and cry9 type genes. no differences that could explain the superior activity of s76 when compared to hd-1, the lepidoptera standard strain, were observed. nevertheless, its higher entomopathogenic activity has pointed this strain s76 as a potential source of cry genes to control sugarcane borer, an important pest that affects sugarcane, a crop that occupies a planted area of about 6 million ha in brazil.
Procura-se Amy: uma musa diferente
Bruschi, Michel Euclides,Medeiros, Patrícia Flores de
Sess?es do Imaginário , 2000,
Abstract:
Manejo de crises asmáticas em crian?as: conhecimento de profissionais de saúde quanto ao tópico em hospitais-escola do Recife (PE)
Lustosa, Giovanna Menezes de Medeiros;Britto, Murilo Carlos Amorim de;Bezerra, Patrícia Gomes de Matos;
Jornal Brasileiro de Pneumologia , 2011, DOI: 10.1590/S1806-37132011000500004
Abstract: objective: knowledge of acute asthma management in children is a subject that has rarely been explored. the objective of this study was to assess the level of such knowledge among health professionals in the city of recife, brazil. methods: this was a cross-sectional survey involving 27 pediatricians and 7 nurses, all with at least two years of professional experience, at two large pediatric teaching hospitals in recife. the participants completed a self-administered multiple-choice questionnaire. results: the pediatricians and nurses all possessed insufficient knowledge regarding the use of metered dose inhalers, nebulization, and types/doses of medications, as well as techniques for decontamination and disinfection of the equipment. conclusions: insufficient knowledge of acute asthma management in children can lead to less effective treatment in hospitals such as those evaluated here. educational programs should be developed in order to minimize this problem.
A mídia como ferramenta de pesquisa: produ??o de saberes no cotidiano sobre a saúde das filhas deste solo
Medeiros, Patrícia Flores de;Guareschi, Neuza Maria de Fátima;
Psicologia & Sociedade , 2008, DOI: 10.1590/S0102-71822008000400012
Abstract: this article aims at discussing media as a research tool considering its knowledge production in everyday life and, therefore, power relations. power is understood as a productive network that traverses the whole social body and produces things, pleasures, ways of knowing, and discourses. we have focused on the television program fantástico, more specifically, on its section dedicated to health, presented by dr. drauzio varella, who presents statistics about the brazilian population and dialogues with women about family planning programs. we intend to make media as a social field, delimiting it as evidence, so to problematize it using foucaultian theoretical tools. thus, media is no longer thought as an evidence that naturalizes the objects it talks about, and lets us discuss it, that is, think of it as something that produces what it talks about.
Testes empíricos sobre o comportamento assimétrico dos custos nas empresas brasileiras
Medeiros, Otávio Ribeiro de;Costa, Patrícia de Souza;Silva, César Augusto Tibúrcio;
Revista Contabilidade & Finan?as , 2005, DOI: 10.1590/S1519-70772005000200005
Abstract: the study tests and confirms the hypothesis that costs of brazilian firms are sticky, i.e. that costs increase with more intensity when revenues increase than in the opposite direction, as shown by recent empirical evidence involving american firms. as opposed to this evidence, however, the sticky behavior does not seem to decrease when periods over one year are considered. the possibility of a partial reversion of the asymmetry when lagged periods are tested is confirmed in the study. the adopted methodology involves different types of panel data regressions. the paper intends to contribute to a better understanding of brazilian firms' cost behavior related to changes in the firms' activity level, which is a relevant theme for business administration, accountants and external financial analysts. by using a sample of 198 firms within a 17-year period, it was found that the properties of the sticky costs model proposed by anderson, banker e janakiraman (2003) are partially applicable to brazil.
Tumour necrosis factor-α, interleukin-2 soluble receptor and different inflammatory parameters in patients with rheumatoid arthritis
Tania Silvia Fr de,Patrícia Tenconi,Marilei Reynaud Debiasi,Yara Santos Medeiros
Mediators of Inflammation , 2002, DOI: 10.1080/0962935021000051539
Abstract: Background and aims: Although the participation of cytokines in the pathogenesis of rheumatoid arthritis (RA) seems to be unequivocal, their relationship with current serum markers of this disease is not clear. The present study analyses whether there is any correlation between the levels of tumour necrosis factor-α (TNF-α), interlukin-2 soluble receptor (sIL-2R) and the concentrations of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and β2-microglobulin in a group of 21 patients with RA, all rheumatoid factor positive.
O papel dos inibidores do fator de necrose tumoral no tratamento da artrite idiopática juvenil
Martin, Patrícia;Medeiros, Ana Cristina de;Goldenstein-Schainberg, Cláudia;
Revista Brasileira de Reumatologia , 2006, DOI: 10.1590/S0482-50042006000200007
Abstract: juvenile idiopathic arthritis (jia) is a chronic disease that may result in permanent disability, requiring sometimes aggressive treatment. while some patients may have an inadequate response to methotrexate and to other immunosuppressive drugs, others do not tolerate them. in these patients tumor necrosis factor inhibitors such as etanercept, infliximab and adalimumab are indicated. multicentric and randomized studies have shown that etanercept is efficacious in disease control, reaching best effects within the first three months of therapy with sustained clinical improvement up to two years of continuous treatment. there are no published controlled studies involving children receiving infliximab and adalimumab, but their effects seem to be similar to that of etanercept. these drugs are relatively safe but it may be observed infectious complications, including tuberculosis and herpes zoster. autoimmune events, lymphoproliferative disorders and other adverse reactions such as headache and abdominal pain barely occur. nevertheless, it is important to emphasize that despite overall beneficial effects of tnf inhibitors, their long term effects remain undefined and therefore indications must be criterious. in addition, the high cost of these drugs limits their use, specially in our country.
Aspectos reprodutivos do cangati, Parauchenipterus galeatus Linnaeus (Osteichthyes, Auchenipteridae) da Lagoa de Extremoz, Rio Grande do Norte, Brasil
Medeiros, Ana Patrícia T. de;Chellappa, Naithirithi T.;Chellappa, Sathyabama;
Revista Brasileira de Zoologia , 2003, DOI: 10.1590/S0101-81752003000400015
Abstract: the lake extremoz is one of the three big lakes in the state of rio grande do norte. the present work was carried out in this lake during the period of october 2000 to september 2001, which included the dry and the rainy seasons. parauchenipterus galeatus linnaeus, 1766 represent 10% of the commercially important species captured from this lake. fish samples were collected on a monthly basis, besides registering the rainfall, temperature, and dissolved oxygen of the lake. the populational structure of cangati and the stage of maturation of the gonads were determined, besides the gonadosomatic relation. the results indicate that the study population presented higher mean values of total length and weight during the dry season. mature fishes were caught during the months of may, june and july which represented the reproductive season.
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