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DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF METOPROLOL SUCCINATE AND ATORVASTATIN CALCIUM IN A PHARMACEUTICAL DOSAGE FORM
Ginoya Charmi G.,Dinesh V. Thakkar
International Research Journal of Pharmacy , 2013,
Abstract: A new, simple, precise, accurate and selective high performance thin-layer chromatographic (HPTLC) method has been developed and validated for the simultaneous determination of Metoprolol succinate and Atorvastatin calcium in a marketed formulation. Chromatographic separation was carried out on Merck TLC aluminium sheets of silica gel 60F254 using Acetonitrile: Methanol: Ethyl acetate: Glacial acetic acid (2: 4: 4: 0.06 % v/v/v/v) as mobile phase followed by densitometric analysis at 223 nm. This system was found to give compact spots for Metoprolol succinate (Rf value of 0.32 ± 0.005) and Atorvastatin calcium (Rf value of 0.77 ± 0.004). The method was validated in terms of linearity, accuracy, precision, limit of detection, limit of quantification and specificity in accordance with International Conference on Harmonization (ICH) guidelines. The calibration curve was found to be linear between 500 to 3000 and 200 to 1200 ng/spot for Metoprolol succinate and Atorvastatin calcium, respectively with significantly high value of correlation coefficient (r2 > 0.99). The limits of detection and quantitation were found to be 0.8432 and 2.5553 ng/spot, respectively for Metoprolol succinate and 27.8428 and 84.3662 ng/spot, respectively for Atorvastatin calcium. The proposed method was found to be accurate, precise, reproducible, specific and sensitive and can be applicable for the simultaneous determination of Metoprolol succinate and Atorvastatin calcium in marketed formulation.
Formulation and Evaluation of Transdermal Patch of Repaglinide
Shailesh T. Prajapati,Charmi G. Patel,Chhagan N. Patel
ISRN Pharmaceutics , 2011, DOI: 10.5402/2011/651909
Abstract:
Morpho-Histological Study of Kidney in Farmed Juvenile Beluga, Huso huso (Linnaeus, 1758)
A. Charmi,M. Bahmani,M.M. Sajjadi,R. Kazemi
Pakistan Journal of Biological Sciences , 2009,
Abstract: In this study, structure, size and distribution of nephron cells on kidney in Huso huso were investigated. The head, body and caudal part of kidney in juvenile Huso huso were sampled. The kidney of Huso huso consisted of glomerulus, proximal, distal and collecting tubule cells. The average area in 1 and 2 year old sturgeon were 2718.07±1387.51 and 2793.89±1348 μm in proximal cells, 2678.80±1249.12 and 2599.98±1428.13 μm in distal cells, 2275.44±1289.52 and 2312.23±1629.58 μm in collecting tubule cells, 4359.8±1573.59 and 5071.04±1916.87 μm in glomerulus and 6019.68±1800.55 and 8307.49±2073.53 μm in Bowman`s capsule, respectively. Average long and small diameter in 1 and 2 year were 68.03±17.82 and 49.94±12.73, 63.29±16.15 and 45.58±12.46 μm in proximal cells, 63.25±16.01 and 44.3±15.09, 63.514±15.25 and 45.46±13.3 μm in distal cells, 51.9±13.04 and 40.54±12.21, 57.08±16.7 and 45.53±15.28 μm in the collecting tubule cells, 91.18±17.93 and 68.72±16.22, 98.7±21.85 and 72.24±17.48 μm in glomerulus and 99.32±19.82 and 76.45±1896, 125.44±24.93 and 93.85±24.78 μm in Bowman`s capsule, respectively. In all cases no statistically significant difference detected in the measured cells among 1 and 2 year old fishes. Morpho-histological pattern of kidney can be developed on the basis of size, feature and distribution of cells in farmed Sturgeon.
Research: PHARMACOGNOSTIC, PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF TWO PIPER SPECIES
Manisha N Trivedi*,Archana Khemani,Urmila D Vachhani,Charmi P Shah
Pharmacie Globale : International Journal of Comprehensive Pharmacy , 2011,
Abstract: Piper species are reported to have great medicinal value in Indian medicine. The current study was therefore carried out to provide requisite pharmacognostic, physicochemical, phytochemical, chromatographic and antimicrobial details about the two piper species namely Piper nigrum and Piper longum. The study will provide referential information for the correct identification of the crude drugs.
A Characterization of the Members of a Subfamily of Power Series Distributions  [PDF]
G. Nanjundan
Applied Mathematics (AM) , 2011, DOI: 10.4236/am.2011.26099
Abstract: This paper discusses a characterization of the members of a subfamily of power series distributions when their probability generating functions satisfy the functional equation where a, b and c are constants and is the derivative of f.
Double Negative Left-Handed Metamaterials for Miniaturization of Rectangular Microstrip Antenna  [PDF]
G. Singh
Journal of Electromagnetic Analysis and Applications (JEMAA) , 2010, DOI: 10.4236/jemaa.2010.26044
Abstract: In this paper, I have explored a significant concept for the miniaturization of microstrip patch antenna configuration by using the double negative (DNG) left-handed Metamaterials, which have dielectric permittivity and magnetic permeability both negative, simultaneously. It is achieved through the concept of phase-compensation by thin slab consist of the double positive (DPS) material, which have dielectric permittivity and magnetic permeability both positive, simultaneously and DNG metamaterials as a substrate of the microstrip patch antenna. By combining the DNG metamaterial slab with the slab made of DPS materials form a cavity resonator whose dispersion relation is independent of the sum of thickness of the slabs filling this cavity but it depends on the ratio of their thicknesses. This cavity constitutes by DPS and DNG material is used as substrate of the microstrip antennas and the DNG material slab is behave as phase compensator.
Dynamic and Configurational Approach to the Glass Transition by Nanoscale Cooperativity  [PDF]
G. Romeo
Open Journal of Biophysics (OJBIPHY) , 2012, DOI: 10.4236/ojbiphy.2012.23012
Abstract: Here we examine the findings obtained for disaccharide/water mixtures near glass transition that involves cooperative relaxation features on kinetic by viscosity and on thermodynamic behaviour by neutron scattering. Then to address cooperative phenomena that mitigate the Debye-Waller behaviour we invoke Adam-Gibbs’ idea of a cooperative rearranging region. Neutron results suggest that the excess mean square displacement behaves as free volume and is closely connected to an elementary step of the structural relaxation. Then viscosity data evidence a breakdown of the Einstein-Debye relation, decoupling attributed to the intermolecular cooperativity.
Confidence Level Estimator of Cosmological Parameters  [PDF]
G. Sironi
Journal of Modern Physics (JMP) , 2012, DOI: 10.4236/jmp.2012.329157
Abstract: Cosmological Models frequently suggest the existence of physical, quantities, e.g. dark energy, we cannot yet observe and measure directly. Their values are obtained indirectly setting them equal to values and accuracy of the associated model parameters which best fit model and observation. Apparently results are so accurate that some researchers speak of precision cosmology. The accuracy attributed to these indirect values of the physical quantities however does not include the uncertainty of the model used to get them. We suggest a Confidence Level Estimator to be attached to these indirect measurements and apply it to current cosmological models.
Mitochondrial DNA sequence analysis in Anabantoidei fish  [PDF]
G. Degani
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.33039
Abstract:

The decline of ornamental fish populations in their native habitats due to over-fishing has brought about the development of ornamental fish aquaculture, to which fish of the Anabantoidei suborder are important contributors. The genetic variations among species of this suborder were examined by mitochondrial gene sequencing analysis using the cytochrome b and 12S genes. According to the cytochrome b gene, the most similar strains were Trichogaster trichopterus (gold) and Trichogaster trichopterus (blue) (100%). Trichogaster leerii was less similar to them (86.0%), and an even lower similarity was found between the species T. trichopterus and Trichogaster labiosus (85.6%). The least similarity was observed between Betta betta and the genera Colisa (50.2%) and Trichogaster (60.1%). The phylogenetic trees of 12S and cytochrome b were very similar. According to 12S, the similarity between Trichogaster species was high (91.4% - 100%), and between species of this genus and Colisa lalia, it was lower (88.4%). In conclusion, sequence analyses, based on cytochrome

Estimation of Parameter in a New Truncated Distribution  [PDF]
G. Nanjundan
Open Journal of Statistics (OJS) , 2013, DOI: 10.4236/ojs.2013.34025
Abstract: This paper discusses the estimation of the parameter in a truncated form of a discrete distribution which is analogous to Burr distribution. The maximum likelihood and the moment estimators of the parameter are obtained. Their asymptotic properties are also established.
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