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Search Results: 1 - 10 of 168363 matches for " Gaspar E.; "
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On the dynamics of relativistic multi-layer spherical shell systems
Merse E. Gaspar,Istvan Racz
Physics , 2011, DOI: 10.1088/0264-9381/28/8/085005
Abstract: The relativistic time evolution of multi-layer spherically symmetric shell systems---consisting of infinitely thin shells separated by vacuum regions---is examined. Whenever two shells collide the evolution is continued with the assumption that the collision is totally transparent. The time evolution of various multi-layer shell systems---comprised by large number of shells thereby mimicking the behavior of a thick shell making it possible to study the formation of acoustic singularities---is analyzed numerically and compared in certain cases to the corresponding Newtonian time evolution. The analytic setup is chosen such that the developed code is capable of following the evolution even inside the black hole region. This, in particular, allowed us to investigate the mass inflation phenomenon in the chosen framework.
Rigidity and flexibility of biological networks
Merse E. Gaspar,Peter Csermely
Computer Science , 2012, DOI: 10.1093/bfgp/els023
Abstract: The network approach became a widely used tool to understand the behaviour of complex systems in the last decade. We start from a short description of structural rigidity theory. A detailed account on the combinatorial rigidity analysis of protein structures, as well as local flexibility measures of proteins and their applications in explaining allostery and thermostability is given. We also briefly discuss the network aspects of cytoskeletal tensegrity. Finally, we show the importance of the balance between functional flexibility and rigidity in protein-protein interaction, metabolic, gene regulatory and neuronal networks. Our summary raises the possibility that the concepts of flexibility and rigidity can be generalized to all networks.
Propriedades moleculares de um isolado brasileiro do Southern bean mosaic virus
Fitopatologia Brasileira , 2002, DOI: 10.1590/S0100-41582002000300009
Abstract: an isolateof the southern bean mosaic virus (sbmv), genus sobemovirus, found in the state of s?o paulo, brazil, was purified and some of its molecular properties determined. the virus particles are 28-30 nm in diameter and the coat protein has a molecular mass of 30 kda. a heterogeneous population of rnas (4.2 kb, 3.1 kb, 2.65 kb, 2.15 kb, 1.64 kb, 1.36 kb and 1.0 kb) was extracted from virus particles, where those of 4.2 kb and 1.0 kb are, respectively, the genomic and the putative subgenomic rna for coat protein. rnas of the same size were also extracted from infected common bean (phaseolus vulgaris) leaves, indicating that they must have some function in the virus replication cycle. only two species of double-stranded rnas (dsrna) were extracted from infected tissues (4.2 kbp and 1.0 kbp), corresponding to the replicative forms of the genomic rna and the subgenomic rna for coat protein. these results indicate that only these two rnas replicate by means of replicative forms (rf) while the others are probably synthesized by an internal initiation of the negative strand of the genomic rna. the sbmv-bsp showed molecular properties analogous to the sbmv described in north america.
Propriedades moleculares de um isolado brasileiro do Southern bean mosaic virus
Fitopatologia Brasileira , 2002,
Abstract: Um isolado do Southern bean mosaic virus (SBMV), gênero Sobemovirus, encontrado em feijoeiro (Phaseolus vulgaris) no Estado de S o Paulo, foi purificado e algumas de suas propriedades moleculares determinadas. As partículas virais apresentam diametro de 28-30 nm e proteína capsidial com massa molecular estimada em 30 kDa. Das partículas virais foi extraído RNA de vários tamanhos (4,2 Kb, 3,1 Kb, 2,65 Kb, 2,15 Kb, 1,64 Kb, 1,36 Kb e 1,0 Kb) sendo aquele de 4,2 Kb o RNA gen mico e o de 1,0 Kb supostamente um subgen mico que codifica para a proteína capsidial. ácidos ribonucleicos de mesmo tamanho foram também detectados in vivo, indicando estar associados à replica o viral. Na análise do RNA de fita dupla (dsRNA), somente duas espécies foram detectadas (4,2 Kpb e 1,0 Kpb) correspondendo às formas replicativas do RNA gen mico e do subgen mico para proteína capsidial. Os resultados indicam que somente estes dois RNA s o replicados por meio de formas replicativas (RFs), enquanto os demais devem ser formados talvez por inicia o interna da fita negativa do RNA gen mico. O SBMV-B SP apresentou propriedades moleculares análogas àquelas do SBMV descrito na América do Norte.
Identifica??o e caracteriza??o de um potyvírus isolado de Zinnia elegans
Maritan, Ana C;Gaspar, José O;Camargo, Luis E. A;
Fitopatologia Brasileira , 2004, DOI: 10.1590/S0100-41582004000100005
Abstract: the present work describes the identification and characterization of a potyvirus isolated from zinnia elegans in the northwest region of the state of s?o paulo, brazil. the zinnia potyvirus was transmitted by mechanical inoculation. its host range was restricted mainly to members of the asteraceae family. by sds-page, the molecular mass of the coat protein (cp) was estimated to be 33 kda and, in western-blot, it reacted with antiserum to bidens mosaic virus (bimv). a fragment of about 820 bp was amplified by rt/pcr, cloned and sequenced. the fragment, which included the cp gene, displayed amino acids similarity in the cp core ranging from 55% (tobacco vein mottling virus, tvmv) to 95% (sunflower chlorotic mottle virus, sucmov). for the whole cp, it ranged from 55% (tvmv) to 91% (sucmov). in the n-terminal region the zinnia potyvirus has a deletion of four amino acids (positions 9 to 12 downstream of the cleavage site between the protein nib and the cp) when compared with the sucmov sequence. filogenetic analysis grouped the zinnia potyvirus and sucmov in the same branch in 100% of the replicates, showing a very close relationship between these two viruses. the results obtained in the present work demonstrate that the zinnia potyvirus and sucmov are strains of the same virus. the name sunflower chlorotic mottle virus, zinnia isolate (sucmov-zi), is suggested for the potyvirus found in z. elegans in brazil.
Detec??o do Grapevine virus A e Grapevine virus B por hibridiza??o "dot-blot" com sondas moleculares n?o radioativas
Moreira, Andreia E;Gaspar, José O;Kuniyuki, Hugo;
Fitopatologia Brasileira , 2005, DOI: 10.1590/S0100-41582005000500015
Abstract: grapevine virus a (gva) and grapevine virus b (gvb) are involved in the kober stem grooving and grapevine corky bark diseases, respectively. this work reports the molecular detection of isolates of gva (gva-sp) and gvb (gvb-c-sp and gvb-i-sp) in grapevines (vitis spp.) and tobacco (nicotiana occidentalis) by non-radioactive molecular probes. the digoxigenin-labeled probes were generated by rt-pcr using specific primers to the coat protein genes. total rna was extracted from 45 plants of several grapevine varieties and from 13 plants of tobacco mechanically inoculated with gvb. the rna extracted from infected plants, considered infected by biological indexing, reacted to the cdna probes while there was no hybridization with healthy plants. these results were also confirmed by rt-pcr experiments. the use of the cdna probes hybridization was proved to be efficient in detecting both gva and gvb with high specificity and sensitivity. however, mature leaves and dormant cuttings should be preferably used in diagnostic tests of gvb and gva, respectively.
As injúrias n?o intencionais no ambiente domiciliar: a casa segura
Paes, Carlos E. N;Gaspar, Vera L. V;
Jornal de Pediatria , 2005, DOI: 10.1590/S0021-75572005000700004
Abstract: objective: to review the characteristics of unintentional injuries and their impact on children and adolescents. sources of data: articles published between 2000 and 2005 in the medline, ebsco, proquest, scielo, bvs and google scholar databases were selected. the authors used the keywords unintentional injuries, injuries, safe home, burns, falls, drowning, scorpions, snakes, poisoning, child, adolescent, mortality, injury control, and hospitalization. some articles were evaluated based on the selected publications. summary of the findings: unintentional injuries in the world and in brazil are analyzed, and so are the behaviors currently adopted for injury prevention and control. the impact on mortality, on physical damage, and the economic burden of injuries are evaluated. special emphasis is placed on home environment, approaching the effects of child development, social disparities and contextualization of home environment on children's world and vulnerabilities. the main types of events that cause physical damage to the child and adolescent in the home environment are described. conclusion: the prevention of injuries in the home environment is possible. in this case, health professionals have the challenge to reduce the consequences of unintentional injuries on the morbidity and mortality of children and young people in brazil and in the whole world.
Estudo de solu??es aquosas de copolímeros em bloco de poli(óxido de etileno)-poli(óxido de propileno) utilizando calorimetria exploratória diferencial (DSC)
Mansur, Claudia R. E.;González, Gaspar;Lucas, Elizabete F.;
Polímeros , 1999, DOI: 10.1590/S0104-14281999000200012
Abstract: the behavior of aqueous systems containing block copolymers of poly(ethylene oxide) (peo) and poly(propylene oxide) (ppo), coupled to hydrocarbon groups, was evaluated by differential scanning calorimetry (dsc). endothermic transitions in the copolymer solutions were observed under a linear heating rate and the initial temperatures of these transitions are in agreement with the cloud points of the copolymers studied. two transitions were observed in the cooling curves; these transitions were better defined at faster cooling rates of the copolymer solutions which can be related to the micelles formation. the effect of the increasing concentration of the hydrotropic agent, sodium p-toluenesulfonate (napts), in the copolymer solutions was observed by the increase in the cloud points given by the dislocation of the endothermic peaks to higher temperatures.
Stellenwert der ambulanten Blutdruck-Langzeitmessung bei Patienten mit Tinnitus
Gaspar L,Jurcova D,Gasparova E,Makovnik M
Journal für Hypertonie , 2010,
Abstract: Obwohl die arterielle Hypertonie ein wichtiger Faktor in der Entstehung und Fortdauer des Tinnitus sein dürfte, zeigen unsere Erfahrungen, dass ihrer Diagnostik zu wenig Aufmerksamkeit gewidmet wird. Wir untersuchten 18 Personen, die unter Tinnitus leiden, mithilfe der ambulanten Blutdruck-Langzeitmessung (ABDM). Zw lf (67 %) von ihnen hatten eine bislang nicht bekannte Hypertonie, die erst durch das ABDM-Verfahren erfasst wurde. Die ABDM sollte deshalb bei allen Patienten mit Tinnitus Anwendung finden und zwar gleich nach dem Beginn sowie auch w hrend der Fortdauer des Tinnitus. M glichen Komplikationen einer arteriellen Hypertonie wie Herzinfarkt, Schlaganfall, Herz- und/oder Nierenversagen k nnte man so potenziell zuvorkommen.
Identifica o e caracteriza o de um potyvírus isolado de Zinnia elegans
Maritan Ana C,Gaspar José O,Camargo Luis E. A
Fitopatologia Brasileira , 2004,
Abstract: O presente trabalho teve como objetivo a identifica o e caracteriza o de um potyvírus isolado de Zinnia elegans, na Regi o Noroeste do Estado de S o Paulo. O potyvírus foi transmitido por inocula o mecanica e apresentou uma gama restrita de hospedeiras sendo que as espécies mais afetadas pertencem à família Asteraceae. Em SDS-PAGE, a massa molecular da proteína capsidial (CP) foi estimada em 33 kDa e, em "Western-blot", reagiu com anti-soro para o Bidens mosaic virus (BiMV). Um fragmento de aproximadamente 820 pb foi amplificado por RT/PCR, clonado e seqüenciado. O fragmento, que inclui o gene da proteína capsidial, mostrou similaridade de aminoácidos do "core" da CP variando de 55% (Tobacco vein mottling virus, TVMV) a 95% (Sunflower chlorotic mottle virus, SuCMoV) e da CP completa de 55% (TVMV) a 91% (SuCMoV). Na regi o N-terminal, o potyvírus de Zinnia tem uma dele o de quatro aminoácidos (posi es 9 a 12 após o sítio de clivagem entre a proteína NIb e a CP) quando comparada com a seqüência do SuCMoV. A análise filogenética agrupou o potyvírus de Zinnia e o SuCMoV em um mesmo ramo em 100% das réplicas, mostrando uma rela o de parentesco muito próxima entre esses dois vírus. Os resultados obtidos no presente trabalho demonstraram que o potyvírus de Zinnia e o SuCMoV s o estirpes do mesmo vírus. Sugere-se o nome Sunflower chlorotic mottle virus, isolado Zinnia (SuCMoV-Zi), ao potyvírus encontrado em Z. elegans no Brasil.
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