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Search Results: 1 - 10 of 325306 matches for " Galbinski Sérgio "
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Viabilidade e Fertiliza o in vitro de Oócitos Bovinos após Vitrifica o em Solu o 6 M de DMSO
Galbinski Sérgio
Revista Brasileira de Ginecologia e Obstetrícia , 2002,
Abstract:
Viabilidade e fertiliza??o in vitro de oócitos bovinos após vitrifica??o
Galbinski, Sérgio;Bos-Mikich, Adriana;Ferrari, Arnaldo Nicola;
Revista Brasileira de Ginecologia e Obstetrícia , 2003, DOI: 10.1590/S0100-72032003000800003
Abstract: purpose: to verify vitrification techniques using 6 m dmso to cryopreserve in vitro matured bovine oocytes, and to assess the effects of the time of exposure to vitrification solutions (vs). methods: dilutions of vs were prepared from the stock vs (vs 100%) consisting of 6 m dmso to give 25 and 65% dmso solutions. bovine oocytes were in vitro matured for 18-22 h. matured oocytes were placed first into 25% vs, at room temperature for 5 min, then transferred to 65% vs, before being pipetted into the 100% vs in plastic straws. three experimental groups were formed: in the first group, time of pipetting through 65% vs and loading the straw took up to 60 s, in the second group it did not exceed 30 s. for thawing, straws were held in air for 10 s and then in a water bath for 10 s. the contents of each straw were expelled in sucrose solution and held for 5 min. in the third experimental group, oocytes went through all vs, but were not vitrified. all retrieved oocytes were inseminated. for control, fresh, in vitro matured oocytes were inseminated. results: after vitrification, 69.1 and 59.8% of the oocytes were retrieved from the 30 s and 60 s groups, respectively, and 93 and 89% of these oocytes appeared morphologically normal 24 h after insemination, respectively. in the group of oocytes exposed without vitrification, 75.6% were retrieved and 84.7% were morphologically viable, 24 h after insemination. no fertilization was observed in the experimental groups. among controls, 65.4% were fertilized. conclusions: the vitrification technique using 6 m dmso is not a feasible approach to cryopreserve in vitro matured bovine oocytes. decreasing the time of exposure to vs did not overcome deleterious effects of the procedure on the fertilizability of oocytes. improvements in the technique are needed to protect the zona pellucida and oolemma.
Viabilidade e fertiliza o in vitro de oócitos bovinos após vitrifica o
Galbinski Sérgio,Bos-Mikich Adriana,Ferrari Arnaldo Nicola
Revista Brasileira de Ginecologia e Obstetrícia , 2003,
Abstract: OBJETIVOS: avaliar a técnica de criopreserva o por vitrifica o em DMSO 6 M para oócitos bovinos maturados in vitro e os efeitos do tempo de exposi o às solu es de vitrifica o (SV). MéTODOS: estudo experimental tipo coorte. Ovários de bovinos foram obtidos em frigorífico e transportados ao laboratório. Os oócitos foram aspirados. A partir da SV contendo DMSO 6 M (SV 100%), foram preparadas solu es a 25 e 65%. Oócitos foram maturados in vitro por 18-22 horas. Para vitrifica o, os oócitos foram colocados em SV 25%, por 5 minutos, transferidos à SV 65%, pipetados em SV 100% para palhetas e estocados em nitrogênio líquido. No primeiro grupo experimental, a exposi o à SV 65% tomou até 60 segundos e no segundo grupo n o ultrapassou 30 segundos. Para descongelamento, as palhetas foram expostas ao ar por 10 segundos, colocadas em banho-maria por 10 segundos e seu conteúdo expelido e mantido em solu o de sacarose por 5 minutos. No terceiro grupo, os oócitos passaram por todas SV menos pelo nitrogênio líquido. Os oócitos recuperados foram inseminados. Para controle, oócitos frescos, maturados in vitro, foram inseminados. RESULTADOS: após vitrifica o, foram recuperados 69,1 e 59,8% dos oócitos nos grupos de 30 segundos e 60 segundos, respectivamente, e 24 horas após insemina o pareceram morfologicamente normais 93 e 89,1% deles, respectivamente. No grupo de oócitos expostos às SV sem vitrifica o, foram recuperados 75,6%, sendo 84,6% destes viáveis 24 horas após insemina o. N o ocorreu fertiliza o nos grupos experimentais. Entre os controles frescos, foram fertilizados 65,4% dos oócitos. CONCLUS ES: a técnica de vitrifica o utilizando DMSO 6 M n o é aplicável para criopreserva o de oócitos bovinos maturados in vitro. A redu o do tempo de exposi o às SV n o superou o efeito deletério sobre a capacidade fertilizadora dos oócitos. Aprimoramentos da técnica s o necessários para prote o da zona pelúcida e do oolema.
Interval Integration Revisited  [PDF]
Sérgio Galdino
Open Journal of Applied Sciences (OJAppS) , 2012, DOI: 10.4236/ojapps.2012.24B026
Abstract: We present an overview of approaches to selfvalidating?one-dimensional integration quadrature formulas and?a verified numerical integration algorithm with an adaptive?strategy. The new interval integration adaptive algorithm delivers?a desired integral enclosure with an error bounded by a specified?error bound. The adaptive technique is usually much more?efficient than Simpson’s rule and narrow interval results can?be reached.
Model of Three-Phase Transmission Line with the Theory of Modal Decomposition Implied  [PDF]
Rodrigo Cleber da Silva, Sérgio Kurokawa
Energy and Power Engineering (EPE) , 2013, DOI: 10.4236/epe.2013.54B217
Abstract:

This paper shows the development of transmission line model, based on lumped element circuit that provides answers directly in the time and phase domain. This model is valid to represent the ideally transposed line, the phases of each of the small line segments are separated in their modes of propagation and the voltage and current are calculated at the modal domain. However, the conversion phase-mode-phase is inserted in the state equations which describe the currents and voltages along the line of which there is no need to know the user of the model representation of the theory in the line modal domain.

Influence of Phosphoric Acid Etching on the Bond Strength of a Universal Adhesive System to Caries-Affected Dentin  [PDF]
Renata Pavanello, Sérgio Luiz Pinheiro
Advances in Biological Chemistry (ABC) , 2018, DOI: 10.4236/abc.2018.83004
Abstract: The purpose of this study was to evaluate the influence of acid etching on the bond strength of a universal adhesive system (Single Bond Universal, 3M) to caries-affected dentin. Forty permanent third molars were selected and carious lesions were induced by the microbiological method with?S. mutans?ATCC25175. Teeth were allocated randomly across four experimental designs (n=10): PA-I: phosphoric acid etching and application of the adhesive system, followed by immediate microtensile bond strength testing; PA-CC: phosphoric acid etching and application of the adhesive system, followed by microtensile bond strength testing after a 14-day cariogenic challenge; NPA-I: application of the adhesive system without acid etching, followed by immediate bond strength testing and NPA-CC: application of the adhesive system without acid etching followed by bond strength testing after 14-day cariogenic challenge. For microtensile bond strength testing,a restoration with Charisma composite resin was made and each specimen was sectioned with a cross-sectional area of 1 mm2. Only adhesive and mixed fractures were considered for bond strength calculation. Results were evaluated by the Kruskal-Wallis and Friedman tests. The highest bond strengths were observed in the phosphoric acid etching groups (p<0.05). Cariogenic challenge did not affect bond strength (p>0.05). The predominant fracture type was adhesive. We conclude that phosphoric acid increased the bond strength of the Single Bond Universal system to caries-affected dentin, and
Sombras como indicadores da percep??o de profundidade
Fukusima, Sérgio S.;
Psicologia: Reflex?o e Crítica , 1997, DOI: 10.1590/S0102-79721997000200008
Abstract: the cast shadow of a flat square was depicted on a computer screen by orthografic projection, and investigated as a perceived depth cue. the shadow was projected to five positions relative to the incidence of the light in each quadrant of the screen. five adults participated in the experiment. results indicated that the magnitude of the depth estimates increased as a function of the exposed shadow area when the same was partially ocluded. in the conditions in which the shadow was not ocluded, perceptual and cognitive factor can bias the depth estimates.
Sombras como indicadores da percep o de profundidade
Fukusima Sérgio S.
Psicologia: Reflex?o e Crítica , 1997,
Abstract: A sombra de um quadrado projetada ortograficamente sobre uma superfície plana foi investigada como indicadora de profundidade percebida. A sombra foi simulada por computador em cinco posi es, relativas ao angulo de incidência da luz, em cada quadrante da tela do monitor. Os resultados indicaram que as magnitudes das estimativas de profundidade aumentam em fun o da área exposta da sombra nos casos em que a mesma é parcialmente ocluída. No caso em que a sombra n o é ocluída, fatores perceptivos e cognitivos podem afetar as estimativas de profundidade.
Feedback boundary stabilization to trajectories for 3D Navier-Stokes equations
Sérgio S. Rodrigues
Mathematics , 2015,
Abstract: Given a nonstationary trajectory of the Navier-Stokes system, a finite-dimensional feedback boundary controller stabilizing locally the system to the given trajectory is derived. Moreover the controller is supported in a given open subset of the boundary of the domain containing the fluid. In a first step a controller is derived that stabilizes the linear Oseen-Stokes system "around the given trajectory" to zero; for that a corollary of a suitable truncated boundary observability inequality, the regularizing property for the system, and some standard techniques of the optimal control theory are used. Then it is shown that the same controller also stabilizes, locally, the Navier-Stokes system to the given trajectory.
Fever and Infection Prevalence in Hematology-Oncology Hospitalized Patients: A Single-Center Retrospective Study  [PDF]
Inês Almeida Costa, Sérgio Chacim, José Mariz
Journal of Cancer Therapy (JCT) , 2018, DOI: 10.4236/jct.2018.93020
Abstract: Fever is prevalent in hospitalized patients. In hematology-oncology patients, it is related with infection, a disease manifestation or a consequence of immunological dysfunction. This single-center retrospective study aims to determine fever prevalence, describe its causes and evaluate the impact of infection on mortality. We reviewed clinical records of 222 patients admitted to the hospital in three random months of 2015. Around 35% of all hospitalizations were complicated by fever, 42.5% of unknown origin. 90.4% were under treatment. Around 81% did antibiotics, most commonly piperacillin/tazobactam, for around 11 days. 41% changed antibiotics due to fever persistence or microbiological results. The diagnostic yield of microbiological tests was 25%; in 66.7%, a Gram-negative microorganism was isolated. We didn’t find an association between infection and mortality. We conclude that fever is common in our hematology-oncology clinic and that the team is trained to assume infection; empirical treatment is in accordance with international recommendations.
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