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Cell death via remote heating of microparticles with potential applications in atherosclerosis and thrombosis therapy
Angelo Gaitas,Gwangseong Kim
PeerJ , 2015, DOI: 10.7287/peerj.preprints.815v1
Abstract: We report a method to cause cell death by remotely heating microparticles by induction heating, this technique could be used to remove vascular deposits and thrombosis. In this preliminary work, we used micrometer size spherical (ferromagnetic) particles and (pure) iron particles to heat remotely macrophages using inductive heating. Iron particles achieved maximum temperatures of 51 ± 0.5 oC after 30 minutes of inductive heating, while spherical particles achieved a maximum temperature of 43.9 ± 0.2 oC (N=6). The therapeutic outcome was determined by monitoring cell re-growth for 2 days following inductive heating treatment. The initial density of cells in the first day prior to induction heating was 105,000 ± 20,820 cells/ml (N=3). 24 hours after induction heating this number was reduced to 6,666 ± 4,410 cells/ml for the spherical particles and 16,666 ± 9,280 cells/ml for the iron particles. The second day the cells grew to 26,667 ± 6,670 cells/ml and 30,000 ± 15,280 cells/ml respectively. Compared to cell cultures with iron and spherical particles that were not subjected to induction heating, we observed a 97% reduction in cell count for the spherical particles and a 91% reduction for the iron particles after the first 24 hours. After 48 hours we observed a 95% reduction in cell growth for both spherical and iron particles. Induction heating of microparticles was highly effective in reducing the macrophage population and preventing their growth.
Chemically modified plastic tube for high volume removal and collection of circulating tumor cells
Angelo Gaitas,Gwangseong Kim
PeerJ , 2015, DOI: 10.7287/peerj.preprints.793v1
Abstract: In this preliminary effort, we use a commercially available and chemically modified tube to selectively capture circulating tumor cells (CTCs) from the blood stream by immobilizing human anti-EpCAM antibodies on the tube's interior surface. We describe the steps required to modify a tube into a cancer cell capturing device. Using these simple modifications, at this proof-of-concept stage of development, we were able to capture about 85% of cancer cells from suspension and 44% of cancer cells from spiked whole blood, the capture percentage being dependent on the tube's length and the number of cancer cells present. Previous work by other researchers has focused on extracting small blood volumes and capturing CTCs with complicated micro-fluidic devices for diagnostic purposes. In addition, prior results of other researchers point to a possible reduction in metastasis achieved by removing CTCs from the bloodstream. We believe that with the utilization of appropriate tube lengths and procedures, we can ensure capture and removal of nearly the entire CTC population in whole blood. Following whole blood flow through the tube, the tube can be trypsinized to release the captured live CTCs for further analysis and testing.
Educa??o, diferen?a e psicologia
Gaitas,Sérgio; Morgado,José;
Análise Psicológica , 2010,
Abstract: the models guiding the intervention of the educational psychologist suggest a multiplicity of opportunities / options that each professional will have to take. research has long suggested that learning together leads all students to better results (e.g., johnson & johnson, 1990, 1994; slavin, 1991). although many psychologists still work only with an individual perspective, the purpose of this paper is to discuss the ways in which the educational psychology can create and sustain authentic inclusion settings. this is based on vigotsky’s belief that all children, in spite of their disabilities, should have access to the regular school system. starting from the analysis of three key aspects inclusion, special education needs and psychology; it concludes with some suggestions that help define how the educational psychology can contribute to the success of all students in the regular school system.
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