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Search Results: 1 - 10 of 97149 matches for " GUO Yang-hao "
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Study of Breeding Saccharomyces cerevisiae with Improved Temperature and Ethanol Tolerance by Genome Shuffling
基因组改组技术选育耐高温、耐高乙醇酿酒酵母菌株的研究

WANG Hao,WANG Hang,MENG Chun,GUO Yang-Hao,
王灏
,王航,孟春,郭养浩

微生物学通报 , 2007,
Abstract: 当以f4、f5、f6作为出发菌株,用酵母菌原生质体紫外诱变的方法,在不同温度下,用含有不同浓度乙醇的平板筛选,分别获得了在耐高温和耐乙醇性状有较大提高的f4.2、f5.1、f6.2、f4.5等正突变菌株。以这些菌株作为出发菌株,进一步用硫酸二乙酯诱变,获得了f5.1.1、f4.2.1两个乙醇耐受性能较高的菌株。在建立了上述不同突变株后,通过基因组改组(genome shuffling)的方法,将上述不同特性的菌株经过两轮genome shuffling,获得了耐高温性能和耐乙醇性能都较好的酵母菌株。经过摇瓶发酵后证明,R24株在35℃发酵过程中,发酵液中的最高乙醇浓度12.93%(W/V),比原始出发菌株f4在35℃的发酵液中最高乙醇浓度8.11%提高了近5%。
The study of epigenetic mechanism of ethanol tolerance genetic instability of yeast
酵母乙醇耐受性状遗传不稳定的表观遗传探讨

MENG Chun,WANG Hang,REN Hong-Zhen,LI Feng,GUO Yang-Hao,
孟春
,王航,任鸿桢,李锋,郭养浩

微生物学通报 , 2011,
Abstract: The improved yeast strains obtained through classical breeding methods, such as the mutation and the acclimatization, easily lost their acquired characteristics during the process of passage or conservation. We investigated the epigenetic molecular mechanism of genetic instability about the yeast acquired traits. The relationship between the genetic stability of ethanol tolerance and the change of H3K4 methylation level of promoters of pro1, tps1, sod1 was studied in the process of yeast strain breeding for improving ethanol tolerance or passage of improved strains without ethanol stress. The results showed that the genetic stability of ethanol tolerance was regulated by epigenetic variation of some genes in the yeast. The genetic instability of acquired traits of yeast might result from its epigenetic variation of relative genes because many environmental factors influenced on the epigenetic molecular modification in cells.
OPTIMIZATION OF TRANSFORMATION SYSTEM OF STREPTOMYCES AUREOFACIENS
金色链霉菌转化系统的优化

MENG Chun,GUO Yang-Hao,YE Qin,SHI Xia-Ai,CHEN Jian-Feng,
金色链霉菌转化系统的优化

微生物学通报 , 2002,
Abstract: 12% sucrose, 0.5% glycin and 1 hour enzymolysis of lysozyme were the optimal conditions for preparation of Streptomyces aureofaciens protoplast. The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium. P-buffer was better than T-buffer in plasmid transformation process. 33% PEG1000 was the fit concentrations for plasmid transformation.
Optimization of Saccharomyces cerevisiae sp. Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase
酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化

YAN Fen,WANG Qian,LIN Zi-Lin,GUO Yang-Hao,
严 芬
,王 茜,林子琳,郭养浩

微生物学通报 , 2008,
Abstract: The culture conditions of Saccharomyces cerevisiae sp.strain by 1.1 b were optimized for the production of D-(-)-mandelate dehydrogenase which is useful for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate.The optimum medium(per liter)consistes of 60 g peptone,30 g maltose, 0.5 g MgSO_4,0.01 g ZnSO_4,1.0 g KCl.After optimization of the culture medium,the enzyme production in shake flasks is enhanced from 2.56 to 20.21 U/L.The optimum fermentation conditions were determined as follows:me...
Optimization of Saccharomyces cerevisiae sp. Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase
酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化

YAN Fen,WANG Qian,LIN Zi-Lin,GUO Yang-Hao,
严 芬
,王 茜,林子琳,郭养浩

微生物学报 , 2008,
Abstract: 对一株产D-(-)-扁桃酸对映选择性脱氢酶的酿酒酵母菌(Saccharomyces cerevisiae sp. strain by1.1b)发酵产酶条件进行了优化。研究各种碳源、氮源及无机盐对产酶的影响, 应用正交试验优化发酵培养基组成, 结果为: 蛋白胨 60 g/L, 麦芽糖 30 g/L, MgSO4 0.5 g/L, ZnSO4 0.01 g/L, KCl 1.0 g/L。优化后酶产量提高了7.9倍(由2.56 U/mL增至20.21 U/mL)。摇瓶培养最佳条件为: 装液量40 %, 发酵pH 6.5, 接种量10 %, 发酵温度30 ℃。考察了细胞生长及产酶的时间进程, 最佳培养时间为25 h。
Effects of 2-phenylethanol on physiological and biochemical characteristics of Saccharomyces cerevisiae
2-苯乙醇对酿酒酵母生理生化特性影响

Wang Hang,Meng Chun,Shi Xian-Ai,Guo Yang-Hao,
王航
,孟春,石贤爱,郭养浩

微生物学通报 , 2012,
Abstract: Objective] To provide useful basis for enhancing the biosynthesis of 2-phenylethanol (PEA), we studied the variation of physiological and biochemical characteristics of Saccharomyces cerevisiae sp. strain R-UV3 treated on varied concentrations of PEA. Methods] Morphological observation was performed by a transmission electron microscope. Membrane permeabilization, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential after staining with propidium iodide, dihydroethidium and rhodamine 123 respectively were investigated at the single-cell level with flow cytometry. The expression of aro10 gene was determined by real-time fluorescence quantitative PCR. Results] With the increasing PEA concentration (0?4.0 g/L), the membrane permeabilization, glycometabolism and expression of aro10 gene in the yeast cell decreased and mitochondrial membrane potential increased. ROS increased when PEA concentration was below 3.0 g/L and decreased when PEA concentration was beyond 3.0 g/L. The physiological and biochemical characteristics of the yeast, such as membrane permeabilization, glycometabolism and expression of aro10 gene, varied notably as PEA concentration increased from 2.4 g/L to 3.0 g/L. Conclusions] The aqueous PEA concentration should be controlled at 2.4?3.0 g/L when in situ PEA removal technology was performed.
Biosynthesis of 2-Phenylethanol by Saccharomyces cerevisiae sp.Strain with ISPR Technology
原位转移技术用于酵母合成2-苯乙醇

GUANG Ang,WANG Hang,MENG Chun,SHI Xian-ai,GUO Yang-hao,
关昂
,王航,孟春,石贤爱,郭养浩

过程工程学报 , 2009,
Abstract: 研究了酵母转化L-苯丙氨酸(Phe)合成2-苯乙醇(Pea)的常规和带原位转移的补料分批培养过程特性. 在常规培养中,以优化补料策略将糖浓度控制在0.1~0.3 g/L,使副产物乙醇浓度小于1%,而Pea的最高终浓度仅为3.85 g/L,因产物抑制效应无法获得更高浓度. 采用大孔树脂FD0816作为原位转移产物的介质,Pea最终总浓度达到12.80 g/L,平均生成速率为0.38 g/-L(h),比未添加树脂的培养体系分别提高了232%和35.7%. 采用乙醇溶液对发酵用的树脂进行动态洗脱,Pea洗脱率达到95%以上,洗脱液中Pea浓度达到60 g/L.
Optimization and Kinetics of the Fed-batch Bioconversion from L-phenylalanine to 2-Phenylethanol via Saccharomyces cerevisiae
酿酒酵母转化生成2-苯乙醇分批补料工艺优化

WANG Hang,DONG Qing-feng,MENG Chun,SHI Xian-ai,GUO Yang-hao,
王航
,董清风,孟春,石贤爱,郭养浩

过程工程学报 , 2010,
Abstract: 对酿酒酵母转化生成2-苯乙醇过程的碳氮源进行优化,选择葡萄糖为碳源,1 g/L脲为氮源,8 g/L L-苯丙氨酸为前体,以呼吸商达1.1±0.1为补糖控制依据,在5 L发酵罐水平建立分批补料工艺,2-苯乙醇终浓度达4.39 g/L,生成速率达0.314 g/(L×h),L-苯丙氨酸摩尔转化率为0.8,L-苯丙氨酸残留浓度仅为0.634 g/L.
Process Characteristics of Bioleaching Meizhou Chalcopyrite by Thermophilic Acidianus brierleyi
嗜热布氏酸菌对梅州黄铜矿的生物浸出过程特性

SHI Xian-ai,LI Cong-ying,LIN Hui,MENG Chun,GUO Yang-hao,
石贤爱
,李聪颖,林晖,孟春,郭养浩

过程工程学报 , 2005,
Abstract: 研究了嗜热布氏酸菌对梅州黄铜矿的浸出机理和浸出过程特性.嗜热布氏酸菌(简称A.B菌)在梅州黄铜矿表面的吸附过程符合朗格缪尔等温线,最大吸附量XAm5.00×108cell/g,吸附平衡常数KA5.88×10?7mL/cell.梅州黄铜矿的浸出主要是通过A.B菌的直接氧化起作用.单独A.B菌处理时,铜浸出速率为0.0137g/(L?h),是Fe3+化学氧化速率的6倍.A.B菌(65℃)对梅州黄铜矿的浸出速率是常温氧化亚铁硫杆菌(简称T.F)的16倍(31℃).A.B菌生长和浸矿的最适温度均为65℃,微生物生长最佳pH为2.0,而浸矿最适pH为1.5.A.B菌处理10d可使铜浸出率达91.3%,具有潜在的工业应用价值.
Control of Phosphate Concentration on Aminoglycoside Antibiotic JI-20A Fermentation
氨基糖苷类抗生素JI-20A发酵过程的磷酸盐控制

CHEN Jian-Feng,SHAO Jing-Wei,ZHANG Yuan-Xing,CHEN Hao,GUO Yang-Hao,
陈剑锋
,邵敬伟,张元兴,陈浩,郭养浩

微生物学通报 , 2007,
Abstract: It was found that the phosphate concentration effected significantly on the cell growth and the production of aminoglycoside antibiotic JI- 20A. Although initial phosphate concentration of 6. 1mmol/L - 9.6 mmol/L was beneficial for the cell growth, the production of aminoglycoside antibiotic JI-20A was inhibited. When the phosphate concentration was controlled below 1.1 mmol/L in the biosynthesis phase of aminoglycoside antibiotic JI-20A, high alkaline phosphatase activity and low pyruvic acid concentration were resulted in, and the production of arninoglycoside antibiotic JI-20A was enhanced.
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