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Search Results: 1 - 10 of 582409 matches for " G.; García-Rivera "
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Dengue severity in the elderly in Puerto Rico
García-Rivera,Enid J.; Rigau-Pérez,José G.;
Revista Panamericana de Salud Pública , 2003, DOI: 10.1590/S1020-49892003000500004
Abstract: objective: severe dengue affects all age groups in the americas, but little detailed information is available about this disease in the elderly. the objective of this article is to describe the disease in this age group. methods: we reviewed suspected dengue-case investigation forms submitted with diagnostic samples as well as clinical reports from infection control nurses in puerto rico, for the period of 1994 through 1999. results: we assigned the laboratory-positive case-patients to four age groups: infants (1 year: 554), youth (2 to 18 years: 6 857), adults (19 to 64 years: 9 433), and elderly (> 65 years: 822). regardless of infecting serotype, the elderly were more likely to have been hospitalized (48% vs. 33%) (p <0.01) and were less likely to show hemorrhage (26% vs. 33%) (p <0.01). on multivariate analysis, controlling for gender and the presence of hemorrhage, the elderly had a higher risk for hospitalization and death than did the youths and the adults. conclusions: the elderly appear to be more likely than youth and younger adults to develop severe illness when infected with the dengue virus, in a pattern similar to that of infants. the clinical evaluation of elderly patients with dengue must include a careful assessment of increased capillary permeability and occult hemorrhage in order to avoid complications from delayed identification and treatment of severe dengue infection. these findings are of increasing importance for dengue epidemiology and medical care in view of the expanding nature of dengue and dengue hemorrhagic fever in a world that also has a growing number and proportion of elderly persons.
Dengue severity in the elderly in Puerto Rico
García-Rivera Enid J.,Rigau-Pérez José G.
Revista Panamericana de Salud Pública , 2003,
Abstract: OBJECTIVE: Severe dengue affects all age groups in the Americas, but little detailed information is available about this disease in the elderly. The objective of this article is to describe the disease in this age group. METHODS: We reviewed suspected dengue-case investigation forms submitted with diagnostic samples as well as clinical reports from infection control nurses in Puerto Rico, for the period of 1994 through 1999. RESULTS: We assigned the laboratory-positive case-patients to four age groups: infants (1 year: 554), youth (2 to 18 years: 6 857), adults (19 to 64 years: 9 433), and elderly (> 65 years: 822). Regardless of infecting serotype, the elderly were more likely to have been hospitalized (48% vs. 33%) (P <0.01) and were less likely to show hemorrhage (26% vs. 33%) (P <0.01). On multivariate analysis, controlling for gender and the presence of hemorrhage, the elderly had a higher risk for hospitalization and death than did the youths and the adults. CONCLUSIONS: The elderly appear to be more likely than youth and younger adults to develop severe illness when infected with the dengue virus, in a pattern similar to that of infants. The clinical evaluation of elderly patients with dengue must include a careful assessment of increased capillary permeability and occult hemorrhage in order to avoid complications from delayed identification and treatment of severe dengue infection. These findings are of increasing importance for dengue epidemiology and medical care in view of the expanding nature of dengue and dengue hemorrhagic fever in a world that also has a growing number and proportion of elderly persons.
Knowledge and attitudes in Puerto Rico concerning dengue prevention
Pérez-Guerra,Carmen L.; Seda,Hilda; García-Rivera,Enid J.; Clark,Gary G.;
Revista Panamericana de Salud Pública , 2005, DOI: 10.1590/S1020-49892005000400005
Abstract: objective: dengue has been endemic in puerto rico for three decades. multiple educational and community-based efforts have been developed to inform the population about dengue prevention. we undertook this study to understand the community members' knowledge, attitudes, and practices related to dengue prevention and to elicit their ideas for future prevention campaigns. methods: a qualitative study based on grounded theory analysis was conducted between february and may of 2001. the study involved a total of 34 participants in four group interviews who had been identified through the puerto rico dengue surveillance system. results: in general, participants had correct knowledge about dengue prevention, but they did not associate the mosquitoes inside their houses with aedes aegypti. participants insisted that "neighbors" needed to control larval habitats, and the participants also asked the government to fumigate. conclusions: the patterns of knowledge and opinion that emerged in the discussions can be arranged along an axis going from high levels of correct knowledge to low levels of correct knowledge about dengue and dengue hemorrhagic fever and related practices. there were few participants at either extreme. three themes explained these patterns: misconceptions about dengue (based on previously delivered information), the "invisibility" of dengue (as compared to other diseases), and responsibility (individual and government). four strategies for preventive behaviors were recommended: developing community groups to identify community priorities on prevention, developing volunteer groups to deliver prevention messages, making house visits to demonstrate specific control measures, and conducting a complementary media campaign to support these strategies.
Utilidad de las formulaciones Vimang? en pacientes con osteoartrosis de rodilla
Valverde,S.; Duarte,E.M.; Ducangé,D.; Garrido,G.; García-Rivera,D.; Jáuregui,U.; Garrido,B.;
Revista de la Sociedad Espa?ola del Dolor , 2009,
Abstract: osteoarthrosis (oa) is the most common form of arthritis and localization in the knee is common. symptomatic slow acting drugs for osteoarthritis include antioxidant agents as possible modifiers of disease progression. vimang? is a registered brand name that covers several formulations of mangifera indica l., used for its antioxidant, antiinfl ammatory, analgesic and immunomodulatory properties. the aim of the present study was to determine the analgesic activity of vimang tablets and cream in patients with knee oa and identify possible posttreatment functional improvement. a further aim was to characterize the effects of vimang on the synovial infl ammation (effusion and proliferation) associated with this entity using ultrasonography. ten patients from hogar santovenia with a clinical-radiological diagnosis of knee oa were studied. mean daily pain scores on an 11-point likert scale were 4 or higher in the week prior to treatment initiation. the western ontario and mcmaster universities (womac) osteoarthritis index was evaluated in the initial consultation and at the end of the study. ultrasonographic examination (aloka device 1100 with a 7.5 mhz transducer) of the soft tissue of both knees was performed. the patients were randomly assigned to three groups. group 1 (n = 3) received a daily dose of 1,800 mg/day (tablets), group 2 (n = 4) received 900 mg/day (tablets) and group 3 (n = 3) received a combination of tablets 900 mg/day and vimang cream 1.2%. the tables were administered in three daily doses every 8 hours and the cream was applied three times per day every 8 hours on the affected knee. treatment duration was 3 months. satisfactory analgesia was achieved in all patients from days 15-21 until 3 months. this effect was at least partly related to the decrease in synovial effusion observed in most affected joints and was independent of the reduction in synovial thickness observed on ultrasound. both effects, the increase and decrease in synovial thickness, wer
Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein
Israel López-Reyes,Guillermina García-Rivera,Cecilia Ba uelos,Silvia Herranz,Olivier Vincent,César López-Camarillo,Laurence A. Marchat,Esther Orozco
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/890674
Abstract: Eukaryotic endocytosis involves multivesicular bodies formation, which is driven by endosomal sorting complexes required for transport (ESCRT). Here, we showed the presence and expression of homologous ESCRT genes in Entamoeba histolytica. We cloned and expressed the Ehvps4 gene, an ESCRT member, to obtain the recombinant EhVps4 and generate specific antibodies, which immunodetected EhVps4 in cytoplasm of trophozoites. Bioinformatics and biochemical studies evidenced that rEhVps4 is an ATPase, whose activity depends on the conserved E211 residue. Next, we generated trophozoites overexpressing EhVps4 and mutant EhVps4-E211Q FLAG-tagged proteins. The EhVps4-FLAG was located in cytosol and at plasma membrane, whereas the EhVps4-E211Q-FLAG was detected as abundant cytoplasmic dots in trophozoites. Erythrophagocytosis, cytopathic activity, and hepatic damage in hamsters were not improved in trophozoites overexpressing EhVps4-FLAG. In contrast, EhVps4-E211Q-FLAG protein overexpression impaired these properties. The localization of EhVps4-FLAG around ingested erythrocytes, together with our previous results, strengthens the role for EhVps4 in E. histolytica phagocytosis and virulence.
EhADH112 Is a Bro1 Domain-Containing Protein Involved in the Entamoeba histolytica Multivesicular Bodies Pathway
Cecilia Ba uelos,Guillermina García-Rivera,Israel López-Reyes,Leobardo Mendoza,Arturo González-Robles,Silvia Herranz,Olivier Vincent,Esther Orozco
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/657942
Abstract: EhADH112 is an Entamoeba histolytica Bro1 domain-containing protein, structurally related to mammalian ALIX and yeast BRO1, both involved in the Endosomal Sorting Complexes Required for Transport (ESCRT)-mediated multivesicular bodies (MVB) biogenesis. Here, we investigated an alternative role for EhADH112 in the MVB protein trafficking pathway by overexpressing 166 amino acids of its N-terminal Bro1 domain in trophozoites. Trophozoites displayed diminished phagocytosis rates and accumulated exogenous Bro1 at cytoplasmic vesicles which aggregated into aberrant complexes at late stages of phagocytosis, probably preventing EhADH112 function. Additionally, the existence of a putative E. histolytica ESCRT-III subunit (EhVps32) presumably interacting with EhADH112, led us to perform pull-down experiments with GST-EhVps32 and [35S]-labeled EhADH112 or EhADH112 derivatives, confirming EhVps32 binding to EhADH112 through its Bro1 domain. Our overall results define EhADH112 as a novel member of ESCRT-accessory proteins transiently present at cellular surface and endosomal compartments, probably contributing to MVB formation during phagocytosis.
Calbindin-D32k Is Localized to a Subpopulation of Neurons in the Nervous System of the Sea Cucumber Holothuria glaberrima (Echinodermata)
Carlos A. Díaz-Balzac, María I. Lázaro-Pe?a, Enrique M. García-Rivera, Carlos I. González, José E. García-Arrarás
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0032689
Abstract: Members of the calbindin subfamily serve as markers of subpopulations of neurons within the vertebrate nervous system. Although markers of these proteins are widely available and used, their application to invertebrate nervous systems has been very limited. In this study we investigated the presence and distribution of members of the calbindin subfamily in the sea cucumber Holothuria glaberrima (Selenka, 1867). Immunohistological experiments with antibodies made against rat calbindin 1, parvalbumin, and calbindin 2, showed that these antibodies labeled cells and fibers within the nervous system of H. glaberrima. Most of the cells and fibers were co-labeled with the neural-specific marker RN1, showing their neural specificity. These were distributed throughout all of the nervous structures, including the connective tissue plexi of the body wall and podia. Bioinformatics analyses of the possible antigen recognized by these markers showed that a calbindin 2-like protein present in the sea urchin Strongylocentrotus purpuratus, corresponded to the calbindin-D32k previously identified in other invertebrates. Western blots with anti-calbindin 1 and anti-parvalbumin showed that these markers recognized an antigen of approximately 32 kDa in homogenates of radial nerve cords of H. glaberrima and Lytechinus variegatus. Furthermore, immunoreactivity with anti-calbindin 1 and anti-parvalbumin was obtained to a fragment of calbindin-D32k of H. glaberrima. Our findings suggest that calbindin-D32k is present in invertebrates and its sequence is more similar to the vertebrate calbindin 2 than to calbindin 1. Thus, characterization of calbindin-D32k in echinoderms provides an important view of the evolution of this protein family and represents a valuable marker to study the nervous system of invertebrates.
mRNA Decay Proteins Are Targeted to poly(A)+ RNA and dsRNA-Containing Cytoplasmic Foci That Resemble P-Bodies in Entamoeba histolytica
Itzel López-Rosas, Esther Orozco, Laurence A. Marchat, Guillermina García-Rivera, Nancy Guillen, Christian Weber, Eduardo Carrillo-Tapia, Olga Hernández de la Cruz, Carlos Pérez-Plasencia, César López-Camarillo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045966
Abstract: In higher eukaryotes, mRNA degradation and RNA-based gene silencing occur in cytoplasmic foci referred to as processing bodies (P-bodies). In protozoan parasites, the presence of P-bodies and their putative role in mRNA decay have yet to be comprehensively addressed. Identification of P-bodies might provide information on how mRNA degradation machineries evolved in lower eukaryotes. Here, we used immunofluorescence and confocal microscopy assays to investigate the cellular localization of mRNA degradation proteins in the human intestinal parasite Entamoeba histolytica and found evidence of the existence of P-bodies. Two mRNA decay factors, namely the EhXRN2 exoribonuclease and the EhDCP2 decapping enzyme, were localized in cytoplasmic foci in a pattern resembling P-body organization. Given that amoebic foci appear to be smaller and less rounded than those described in higher eukaryotes, we have named them “P-body-like structures”. These foci contain additional mRNA degradation factors, including the EhCAF1 deadenylase and the EhAGO2-2 protein involved in RNA interference. Biochemical analysis revealed that EhCAF1 co-immunoprecipitated with EhXRN2 but not with EhDCP2 or EhAGO2-2, thus linking deadenylation to 5′-to-3′ mRNA decay. The number of EhCAF1-containing foci significantly decreased after inhibition of transcription and translation with actinomycin D and cycloheximide, respectively. Furthermore, results of RNA-FISH assays showed that (i) EhCAF1 colocalized with poly(A)+ RNA and (ii) during silencing of the Ehpc4 gene by RNA interference, EhAGO2-2 colocalized with small interfering RNAs in cytoplasmic foci. Our observation of decapping, deadenylation and RNA interference proteins within P-body-like foci suggests that these structures have been conserved after originating in the early evolution of eukaryotic lineages. To the best of our knowledge, this is the first study to report on the localization of mRNA decay proteins within P-body-like structures in E. histolytica. Our findings should open up opportunities for deciphering the mechanisms of mRNA degradation and RNA-based gene silencing in this deep-branching eukaryote.
Efecto de los tratamientos antirretrovirales en las manifestaciones orales de los pacientes VIH+
Jané-Salas,E.; Chimenos-Küstner,E.; López-López,J.; Roselló-Llabrés,X.; Oca?a-Rivera,I.;
Avances en Odontoestomatología , 2006, DOI: 10.4321/S0213-12852006000600003
Abstract: we evaluate the effects of antiretroviral treatments in hiv seropositive patients, who were seen in the clinic for infectious diseases in the vall d′hebron hospital, barcelona. 90 patients were seen, 51 males (56.7%) and 39 females (43.3%), the mean age of the patients was 36.2 years with a deviation of ±17.8, and a mode of 35 years. the visits were carried out between january and december 1999. these patients were receiving antiretroviral treatment with one or more drugs and some of them (32.2%) were subjected to high activity antiretroviral treatment (haart). also, in these patients the cd4 count and bimodal viral load was measured, at the start of the disease process and at the time of visit. theoral cavity was examined at this time, in search of lesions associated with aids according to the classification by pindborg et al, 1989. the most prevalent pathology found in this study was the sensation of dry mouth or xerostomia in 47.8%, followed by, in order of frequency, multiple caries (34.4%) and erythematous candidiasis (31.1%). other oral manifestations are very rare, in comparison with the results of other authors in the time before the use of protease inhibitors (pi). there is, for example, only one case of leukoplakia, so characteristic before 1996.
Acute Toxicity and Genotoxic Evaluation of Metlin® and Metlos® (Organic Agave Fructans)  [PDF]
M. I. Gracia, M. M. Tinoco, H. M. Rivera, B. F. Sanchez, P. G. Tapia, L. M. Altamirano, R. L. Romero, O. L. García
Food and Nutrition Sciences (FNS) , 2013, DOI: 10.4236/fns.2013.47A013
Abstract:

The aim of the study was to contribute to the information on agave soluble fibers since research has been focused on chicory fiber but not in agave products; thus we assess the acute toxicity and genotoxicity of two organic and high purity dietary soluble fibers from agave, Metlin? and Metlos?. We performed an acute toxicity assay in Hsd:ICR mice and Hsd:Wistar rats and an in vivo genotoxic test. Results showed that there are no deaths at any doses or genotoxicity, so it can be concluded that these products are non-toxic, at the administrated doses and none showed a cytotoxic, clastogenic or aneuploidic effect.

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