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Search Results: 1 - 10 of 14718 matches for " Fushan Tang "
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The Amphiphilic Self-Assembling Peptide EAK16-I as a Potential Hydrophobic Drug Carrier
Jing Wang,Fushan Tang,Feng Li,Juan Lin,Yinghui Zhang,Linfang Du,Xiaojun Zhao
Journal of Nanomaterials , 2008, DOI: 10.1155/2008/516286
Abstract: It is crucial for hydrophobic drugs to be dissolved and stabilized by carriers in aqueous systems and then to be delivered into target cells. An amphiphilic self-assembling peptide EAK16-I (Ac-AEAKAEAKAEAKAEAK-NH2) is reported here to be able to stabilize a model hydrophobic compound, pyrene, in aqueous solution, resulting in the formation of colloidal suspensions. Egg phosphatidylcholine (EPC) vesicles are used as plasma membranes mimic. Fluorescence data shows that the pyrene is presented in the crystalline form when stabilized by EAK16-I and molecularly migrates from its peptide encapsulations into the membrane bilayers of EPC vesicles when the suspension is mixed with EPC vesicles. Furthermore, the release rate can be controlled by changing peptide-to-pyrene ratio, and the higher ratios lead to the slower release rates due to a thicker encapsulation on the pyrene microcrystals. This demonstrates that EAK16-I, as a promising nanobiomaterial, has the potential to be a hydrophobic compounds carrier.
A Justification of Two-Dimensional Nonlinear Viscoelastic Shells Model
Fushan Li
Abstract and Applied Analysis , 2012, DOI: 10.1155/2012/287865
Abstract: By applying formal asymptotic analysis and Laplace transformation, we obtain two-dimensionalnonlinear viscoelastic shells model satisfied by the leading term of asymptotic expansion of the solutionto the three-dimensional equations.
A Constructive Study of English and Chinese Double Negation
Fushan Sun
Theory and Practice in Language Studies , 2011, DOI: 10.4304/tpls.1.10.1442-1445
Abstract: As a universally used linguistic structure, double negation has long attracted the attention of scholars in Chinese and foreign countries. But so far the research of double negation is limited to the study and analysis of negative words, negative markers and the surface structure of double negation, and is also restricted to one particular language. In this essay, based on contrastive linguistics and cognitive linguistics, I launch a comparison between English double negation and its Chinese counterpart from the cognitive perspective to identify similarities and differences between them, and furthermore I also attempt to elaborate the causes and motivations for its meaning.
Pedagogical Implications to Teaching English Writing
Fushan Sun
Journal of Language Teaching and Research , 2010, DOI: 10.4304/jltr.1.6.867-870
Abstract: English, as an international language, takes such a position in foreign language teaching and learning in China. As we all know that the ultimate purpose of language teaching is to cultivate students’ communicative ability including not only the oral ability, but also the writing ability. However, teaching of English writing is far from satisfactory, and many students still have a great difficulty in writing English composition. They find it hard to express their ideas and feeling freely and fluently, also they make many mistakes in vocabulary and grammar. Even though there are few grammar mistakes in the composition, it still seems more or less odd when read by native speakers. Teachers should make corresponding changes of the emphasis and pedagogy in their teaching and thus provide the most effective way to ease the students in the writing process and motivate them to write. The aim of this paper is to provide pedagogical implications to help the students to improve their English writing competence.
Asymptotic Energy Estimates for Nonlinear Petrovsky Plate Model Subject to Viscoelastic Damping
Xiuli Lin,Fushan Li
Abstract and Applied Analysis , 2012, DOI: 10.1155/2012/419717
Abstract: We consider the nonlinear Petrovsky plate model under the presence of long-time memory. Under suitable conditions, we show that the energy functional associated with the equation decays exponentially or polynomially to zero as time goes to infinity.
MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
Alexey A Fushan, Dennis T Drayna
BMC Biotechnology , 2009, DOI: 10.1186/1472-6750-9-83
Abstract: In this study we developed a quick and efficient strategy for introduction of multiple complex mutations in a target DNA without intermediate subcloning by using a combination of connecting SDM and suppression PCR. The procedure consists of sequential rounds, with each individual round including PCR amplification of target DNA with two non-overlapping pairs of oligonucleotides. The desired mutation is incorporated at the 5' end of one or both internal oligonucleotides. DNA fragments obtained during amplification are mixed and ligated. The resulting DNA mixture is amplified with external oligonucleotides that act as suppression adapters. Suppression PCR limits amplification to DNA molecules representing full length target DNA, while amplification of other types of molecules formed during ligation is suppressed. To create additional mutations, an aliquot of the ligation mixture is then used directly for the next round of mutagenesis employing internal oligonucleotides specific for another region of target DNA.A wide variety of complex multiple mutations can be generated in a short period of time. The procedure is rapid, highly efficient and does not require special chemical reagents. Thus, MALS represents a powerful alternative to the existing methods for multiple SDM.The introduction of multiple mutations in the same gene is often necessary for a wide range of studies, including studying gene expression and protein structure and function. Thus, site-directed mutagenesis is a central method in molecular biology. A number of strategies have been developed to simplify the generation of multiple mutant sites within a target sequence [1,2].A popular approach employs several pairs of mutagenic primers for sequential rounds of mutagenesis. This procedure is robust, however it is time-consuming because it requires a subcloning procedure between rounds of mutagenesis [3]. Another strategy utilizes combining mutagenic oligonucleotides in the same reaction [4-7]. Non-PCR based
Studies on the Distribution of Wild Soybean (Glycine soja) in China
Kejing Wang,Fushan Li,Akbar Ali Cheema
Pakistan Journal of Biological Sciences , 2001,
Abstract: Investigations showed that wild soybean grew in a vast area from about 24 N (middle-northern Guangdong Province and Guangxi Zhuang Autonomous Regions) in the south to 53 N (Heilongjiang River valley) in the northern China, and that there was no wild soybean to find in three regions Xinjiang, Qinghai and Hainan. The area of 30 to 45 N was a diverse region of the wild soybean in China, where the wild soybean has an extensive distribution, large populations and rich types. The highest point of wild soybean distribution in China was 2670 m, locating at 27 30` N, Ninglang County, Yunnan Province. Two critical meteorological indexes related temperature and humidity was key factors to influence the distribution of wild soybean in China: no wild soybeans were found in the areas having a monthly mean temperature of below 20 C in the warmest months or lasting seven months and over of more than 20 C monthly mean temperature, and having an annual precipitation of less than 300 mm during a year.
Dosimetric Study of Coplanar and Non-Coplanar Intensity-Modulated Radiation Therapy Planning for Esophageal Carcinoma  [PDF]
Ying Li, Bing Liu, Fushan Zhai, Yongfeng Yang, Ming Liu, Chaoen Bao, Qingxiang Zhou
International Journal of Medical Physics,Clinical Engineering and Radiation Oncology (IJMPCERO) , 2013, DOI: 10.4236/ijmpcero.2013.24018
Abstract: Purpose: To compare the dosimetric impact of coplanar intensity modulated radiation therapy (IMRT) and non-coplanar IMRT for the esophageal carcinoma. Methods: There are forty-five esophageal carcinoma patients, fifteen of whom were cervical and upper thoracic (Group 1) and thirty were middle and lower thoracic (Group 2). Gross tumor volume (GTV), clinical target volume (CTV), and organs at risk (OAR) were contoured by the chief physician in the CMS-XiO treatment planning system. For each patient, one coplanar plan and two non-coplanar plans have been created using the same physical objective function. A detailed dose-volume histogram (DVH) comparison among three plans was then carried out in a tabulated format. Results: 1) In Group 1 patients with PTV volume less than 100cc, the mean dose and dose gradient of non-coplanar plan were much better than those in coplanar plan. 2) In Group 2 patients, the conformity index (CI) for coplanar and two non-coplanar plans were 0.69 ± 0.13, 0.41 ± 0.13, and 0.68 ± 0.15, respectively. The V5, V10, V20, and the mean dose to the lung were lower in the non-coplanar plans compared to ones in coplanar plan. However, the non-coplanar plans resulted in an increase in a dose to the heart, but the dose was still within heart toxicity tolerance. Conclusion: For Group 1 patients, the
Study on Cellulase Gene Expressed in Pichia pastoris and Analyses of Its Biochemical Characters  [PDF]
Min Lu, Juan Chang, Ping Wang, Qingqiang Yin, Xiaowei Dang, Tianzeng Gao, Fushan Lu
Journal of Materials Science and Chemical Engineering (MSCE) , 2018, DOI: 10.4236/msce.2018.67006
Abstract:
Objectives: In order to increase cellulose degradation, cellulase was expressed in this study. Literature Review: Cellulose is the most abundant organic carbon source on Earth; its enzymatic hydrolysis will be very useful for bioenergy production and resource recycling. Methods: Cellobiohydrlase I (CBH I) gene was amplified from genomic DNA of Trichoderma koningii and inserted into pGAPZα A plasmid to construct the vector of pGAPZαA-CBH I. It was linearized and transformed into Pichia pastoris by electroporation. The recombinant Pichia pastoris was selected and incubated with YPD medium for cellulase secretion. Results: The result showed that CMCase and avicelase activity in the supernatant was 1.1798 U/mL and 0.1276 U/mL, the molecular weight of the expressed protein was 53 kDa determined with SDS-PAGE analyses, and the optimal temperature and pH of the expressed cellulase were 45?C - 50?C and 4.5 - 5.0, respectively. Conclusion: Cellulase gene from T. koningii has been successfully cloned and expressed in Pichia pastoris.
Primary Reasoning behind the Double ITCZ Phenomenon in a Coupled Ocean-Atmosphere General Circulation Model
LI Jianglong,ZHANG Xuehong,YU Yongqiang,DAI Fushan,
LI Jianglong
,ZHANG Xuehong,YU Yongqiang,DAI Fushan

大气科学进展 , 2004,
Abstract: This paper investigates the processes behind the double ITCZ phenomenon, a common problem in Coupled ocean-atmosphere General Circulation Models (CGCMs), using a CGCM-FGCM-0 (Flexible General Circulation Model, version 0). The double ITCZ mode develops rapidly during the first two years of the integration and becomes a perennial phenomenon afterwards in the model. By way of Singular Value Decomposition (SVD) for SST, sea surface pressure, and sea surface wind, some air-sea interactions are analyzed. These interactions prompt the anomalous signals that appear at the beginning of the coupling to develop rapidly. There are two possible reasons, proved by sensitivity experiments: (1) the overestimated east-west gradient of SST in the equatorial Pacific in the ocean spin-up process, and (2) the underestimated amount of low-level stratus over the Peruvian coast in CCM3 (the Community Climate Model, Version Three). The overestimated east-west gradient of SST brings the anomalous equatorial easterly. The anomalous easterly, affected by the Coriolis force in the Southern Hemisphere, turns into an anomalous westerly in a broad area south of the equator and is enhanced by atmospheric anomalous circulation due to the underestimated amount of low-level stratus over the Peruvian coast simulated by CCM3. The anomalous westerly leads to anomalous warm advection that makes the SST warm in the southeast Pacific.The double ITCZ phenomenon in the CGCM is a result of a series of nonlocal and nonlinear adjustment processes in the coupled system, which can be traced to the uncoupled models, oceanic component, and atmospheric component. The zonal gradient of the equatorial SST is too large in the ocean component and the amount of low-level stratus over the Peruvian coast is too low in the atmosphere component.
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