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Search Results: 1 - 10 of 160302 matches for " Fu-feng Li "
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Metabolite changes in the greasy tongue coating of patients with chronic gastritis
Fu-feng Li
Zhong Xi Yi Jie He Xue Bao , 2012,
Abstract: OBJECTIVE: To explore the changes in metabolites in the greasy tongue coating in patients with chronic gastritis.METHODS: Forty chronic gastritis patients presenting with greasy tongue coating, 30 chronic gastritis patients presenting with non-greasy tongue coating, and 20 healthy control persons presenting with light red tongues and thin white coating were enrolled, and the tongue coating was detected by combining artificial diagnosis and the Z-BOX Tongue Digital Analyzer’s diagnosis. Samples of all the tongue coatings were collected before treatment. The metabolic fingerprinting of the tongue coating samples was obtained using ultra-performance liquid chromatography and mass spectrometry (UPLC-MS), and the metabolic components in the tongue coating samples were detected. After this, principal component analysis, partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to identify potential metabolic markers. Finally, the components were identified using the Chemspider and HMDB searching.RESULTS: UPLC-MS results were analyzed by OPLS-DA and showed that the metabolites among the three groups were distributed in different regions. The different potential metabolic markers between the patients with or without greasy coating were 3-ketolactose, 2-deoxy-D-ribose, UDP-D-galactose metarhodopsin, ascorbate, picolinate and histidine. The different potential metabolic markers between the greasy coating group and the normal group were 3-ketolactose, UDP-D-galactose, leukotriene A4 and vitamin D2.CONCLUSION: The metabolites of the greasy coating group, the non-greasy coating group and the normal group show significant differences in energy metabolism, mainly of glucose metabolism. This demonstrated that glucose metabolism may be one of the mechanisms leading to the formation of greasy coating.
Immunological mechanism of exfoliative tongue fur in children with asthma
LI Fu-Feng
Zhong Xi Yi Jie He Xue Bao , 2005,
Abstract: Objective: To explore the immunological mechanism of exfoliative tongue fur in children with asthma. Methods: Thirty-nine children with asthma, twenty-eight children with repetitive respiratory tract infection (non-asthma) and eleven healthy children were divided into five groups, which were asthma with exfoliative fur or with non-exfoliative fur groups, non-asthma with exfoliative fur or with non-exfoliative fur groups and normal control group. The concentrations of keratin 13 and bcl-2 in cells exfoliated from tongue fur were detected by immunohistochemical method. The expression levels of blood cell chemokine receptor-3 (CCR-3) and CD4+ were examined by flow cytometry, and the levels of serum cortisol and IgE were detected by radioimmunoassay. Results: The levels of blood CD4+ and CCR-3 of children with asthma and exfoliative fur were higher than those in the asthma with non-exfoliative fur group and the normal control group (P<0.05). The serum level of cortisol in the groups of asthma with exfoliative fur and non-asthma with exfoliative fur were lower than that in the other groups (P<0.05). The serum levels of IgE in asthma with exfoliative fur or with non-exfoliative fur groups were higher than that in the other groups (P<0.05). Concentrations of keratin 13 in the cells exfoliated from tongue fur in the groups of asthma or non-asthma with exfoliative fur were lower than that of the other groups (P<0.05). There was no significant difference of expression level of bcl-2 in the cells exfoliated from tongue fur among these five groups. Conclusion: There is a reasonably close relationship between the formation of exfoliative tongue fur and the immune system such as low level of serum cortisol and high levels of blood CD4+ and CCR-3, which may all promote the formation of exfoliative fur. The disability of keratinization and apoptosis of epithelial cells of tongue may also be one cause for its formation
Fu-feng Yan,Chong-jia Liang
Acta Crystallographica Section E , 2009, DOI: 10.1107/s1600536809046248
Abstract: In the title molecule, C12H15NS2, the 1,3-thiazinane ring has a half-boat conformation; the C atom at position 5 deviates by 0.715 (2) from the mean plane (P) of the remaining five atoms. Plane P and the phenyl ring form a dihedral angle of 83.62 (3)°. In the crystal structure, weak intermolecular C—H...S hydrogen bonds link molecules related by translation along the axis a into chains.
Fu-Feng Yan,Chong-Jia Liang
Acta Crystallographica Section E , 2009, DOI: 10.1107/s1600536809046352
Abstract: In the title compound, C12H13Cl2NS2, the thiazinane ring adopts a half-boat conformation. An intramolecular C—H...S hydrogen bond is observed. In the crystal structure, centrosymmetrically related molecules interact through an aromatic π–π stacking interactions, with a centroid–centroid separation of 3.790 (2) .
Molecular Basis for the Dissociation Dynamics of Protein A-Immunoglobulin G1 Complex
Fu-Feng Liu, Bo Huang, Xiao-Yan Dong, Yan Sun
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066935
Abstract: Staphylococcus aureus protein A (SpA) is the most popular affinity ligand for immunoglobulin G1 (IgG1). However, the molecular basis for the dissociation dynamics of SpA-IgG1 complex is unclear. Herein, coarse-grained (CG) molecular dynamics (MD) simulations with the Martini force field were used to study the dissociation dynamics of the complex. The CG-MD simulations were first verified by the agreement in the structural and interactional properties of SpA and human IgG1 (hIgG1) in the association process between the CG-MD and all-atom MD at different NaCl concentrations. Then, the CG-MD simulation studies focused on the molecular insight into the dissociation dynamics of SpA-hIgG1 complex at pH 3.0. It is found that there are four steps in the dissociation process of the complex. First, there is a slight conformational adjustment of helix II in SpA. This is followed by the phenomena that the electrostatic interactions provided by the three hot spots (Glu143, Arg146 and Lys154) of helix II of SpA break up, leading to the dissociation of helix II from the binding site of hIgG1. Subsequently, breakup of the hydrophobic interactions between helix I (Phe132, Tyr133 and His137) in SpA and hIgG1 occurs, resulting in the disengagement of helix I from its binding site of hIgG1. Finally, the non-specific interactions between SpA and hIgG1 decrease slowly till disappearance, leading to the complete dissociation of the SpA-hIgG1 complex. This work has revealed that CG-MD coupled with the Martini force field is an effective method for studying the dissociation dynamics of protein-protein complex.
Experimental Investigation on Flow Field Structure of Unsteady Cavitation Flow

HUANG Biao,WANG Guo-yu,WANG Fu-feng,GAO De-ming,

实验力学 , 2011,
Abstract: Flow field structure of unsteady cavitation flow around a Clark-Y type hydrofoil was experimentally investigated.Experiments were carried out in a rectangular test section of a cavitation tunnel.A high-speed video camera was used to visualize the unsteady cloud cavitation flow pattern,and a particle image velocimetry(PIV) was also used to measure instantaneous velocity field and vortex field in cavitation flow field around the hydrofoil.Experimental results show that cavitation phenomennon has an important ...
Study on the Comprehensive Financial Performance and Provincial Difference of Travel Agency Industry of China—Based on Catastrophe Progression Theory and Entropy Method  [PDF]
Yunxin Fu, Qiong Li, Haiyan Feng
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.52008
Abstract: The performance of travel agencies has always been the focus and its quantitative evaluation is also an important study field. From financial view, the catastrophe progression method and the entropy method are used to build a evaluation indicator system of comprehensive financial performance for travel agency industry applying statistical data of the major financial indicator of travel agency from 2005 to 2012, and then empirical research on comprehensive financial performance of travel agencies from 30 different provinces, except Hong Kong, Macao and Taiwan regions and Tibet is made. The result indicates that when the financial performance of travel agency is measured, profitability is the most important indicator, followed by development, solvency and asset management capabilities; through empirical research, the financial performance of travel agencies from 30 different provinces is evaluated and ranked. Finally the reasons are discussed.
On the Integration Schemes of Retrieving Impulse Response Functions from Transfer Functions
Kui Fu Chen,Yan Feng Li
Mathematical Problems in Engineering , 2010, DOI: 10.1155/2010/143582
Abstract: The numerical inverse Laplace transformation (NILM) makes use of numerical integration. Generally, a high-order scheme of numerical integration renders high accuracy. However, surprisingly, this is not true for the NILM to the transfer function. Numerical examples show that the performance of higher-order schemes is no better than that of the trapezoidal scheme. In particular, the solutions from high-order scheme deviate from the exact one markedly over the rear portion of the period of interest. The underlying essence is examined. The deviation can be reduced by decreasing the frequency-sampling interval. 1. Introduction Some linear dynamic systems are described at first in the frequency domain (FD) via the frequency response function (FRF) or transfer function . For example, the characteristic of an unbounded media is relatively conveniently described in FD, as are the cases of soil-structure interaction and crack analysis [1–6]. Another salient example is where the transfer function is modified directly in FD to match some special material properties, as is the case of a hysteretic damping model [7–12]. The media property of attenuating wave propagation is also easily described via the FD expression [13]. The unitary impulse response function (UIRF) is the inverse Fourier transform of the FRF as or the inverse Laplace transform Here the real number is the convergence abscissa, that is, all the poles lie on the left side of . Though and are equivalent for describing a linear time invariant system, sometimes, is preferred, such as when inspecting the system causality, or computing in time domain [3, 7, 8, 12, 14]. For simple cases, the transform from to can be carried out analytically [15–17]. But from an engineering point of view, a numerical approach is recommended, especially when the closed-form solutions do not exist, for example, in the case of an ideal hysteretic model [8–11]. The numerical inverse Laplace transform (NILT) appears in many engineering problems [18, 19], and lots of algorithms have been constructed. Novel approaches are still developing, for example, Wang’s approach based on the wavelet [20]. The first issue in implementing the NILT is the infinite integral bound of (1.2). This is addressed by choosing a large enough to accommodate the essential part of , and ignoring contribution beyond the , that is, Provided that attenuates very fast as , and is large enough, then the approximation (1.3) is acceptable. Otherwise, if attenuates slowly, then, even if is very small for , we cannot ignore the truncating error arbitrarily, because
Expression and localization of the spore wall protein SWP26 of Nosema bombycis in the silkworm BmN cell line  [PDF]
Feng Zhu, Zhongyuan Shen, Shengyan Xiao, Yajie Yue, Xuliang Fu, Xudong Tang, Li Xu, Xijie Guo
Agricultural Sciences (AS) , 2013, DOI: 10.4236/as.2013.42013

The microsporidian spore wall proteins, as the main components of the spore wall, play a key role in spore adherence to host cells and in recognition of the parasite by the host during the invasion process. In this study, we used the Bac-to-Bac baculovirus expression system to express the spore wall protein SWP26, fused to enhanced green fluorescent protein (EGFP), in the silkworm BmN cell line. The SWP26 and EGFP genes were inserted into the baculovirus transfer vector pFastBac1. The transfer vector pFastBac1-swp26-egfp was transformed into the bacterium Escherichia coli DHl0Bac/Bombyx mori nucleopolyhedrovirus (BmNPV) to construct the recombinant vBmswp26-egfp bacmid. The vBmswp26-egfp bacmid DNA was then used to transfect BmN cells to obtain the recombinant baculovirus. Western blotting analysis of total protein lysates in BmN cells infected by the recombinant virus showed a protein band of approximately 51 kDa, which corresponded to the deduced molecular weight of the swp26-egfp fusion protein. In addition, a fluorescence signal was observed in the cytoplasm and nucleoplasm of transfected cells, indicating that SWP26 had been successfully expressed in BmN cells. The SWP26 expression system established in this study lays the foundation for additional molecular and cellular studies, especially those focused on the interaction between the SWP26 protein of Nosema bombycis and the proteins of the silkworm, Bombyx mori.

On coordinated development of oasis and environment in arid area

FU Xiao-feng,LI Zhe,

地理学报 , 2001,
Abstract: Based upon the formation and evolution of oasis and the factors restricting the coordinated development of oasis economy and environment, this paper presents a goal of the development in coordination. It suggests that the sustainable survival and development of oasis could be ensured only if the oasis-desert and water source ecology are managed in a combined way to form a macro system. In light with the above mentioned, the approach to the development of economy and environment of oasis in arid area should depend upon the establishment of an oasis ecological and economic system, which suits the arid environment and promotes the efficiency of resource configuration, stabilizes economic increment and benefits ecological development.
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