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Search Results: 1 - 10 of 316952 matches for " Francisco J. Boye "
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Arch Reconstruction in Hypoplastic Left Heart Syndrome: Handling the Diminutive Aorta  [PDF]
Francisco J. Boye, Frank A. Pigula
World Journal of Cardiovascular Surgery (WJCS) , 2013, DOI: 10.4236/wjcs.2013.36039
Abstract: The diminutive aorta presents technical challenges in the palliation of hypoplastic left heart syndrome. Furthermore, aortic arch caliber changes and variable great vessel relationships can add complexity to an already difficult arch repair. We describe a technical approach that simplifies the aortic reconstruction and makes the procedure more generalizable and reproducible.
A high-resolution record of carbon accumulation rates during boreal peatland initiation
J. Bown, M. Boye,D. M. Nelson
Biogeosciences (BG) & Discussions (BGD) , 2012,
Abstract: The organic speciation of dissolved cobalt (DCo) was investigated in the subtropical region of the southeastern Atlantic, and in the Southern Ocean in the Antarctic Circumpolar Current (ACC) and the northern Weddell Gyre, between 34°25′ S and 57°33′ S along the Greenwich Meridian during the austral summer of 2008. The organic speciation of dissolved cobalt was determined by competing ligand exchange adsorptive cathodic stripping voltammetry (CLE-AdCSV) using nioxime as a competing ligand. The concentrations of the organic ligands (L) ranged between 26 and 73 pM, and the conditional stability constants (log K'CoL) of the organic complexes of Co between 17.9 and 20.1. Most dissolved cobalt was organically complexed in the water-column (60 to >99.9%). There were clear vertical and meridional patterns in the distribution of L and the organic speciation of DCo along the section. These patterns suggest a biological source of the organic ligands in the surface waters of the subtropical domain and northern subantarctic region, potentially driven by the cyanobacteria, and a removal of the organic Co by direct or indirect biological uptake. The highest L:DCo ratio (5.81 ± 1.07 pM pM 1) observed in these surface waters reflected the combined effects of ligand production and DCo consumption. As a result of these combined effects, the calculated concentrations of inorganic Co ([Co']) were very low in the subtropical and subantarctic surface waters, generally between 10 19 and 10 17 M. In intermediate and deep waters, the South African margins can be a source of organic ligands, as it was suggested to be for DCo (Bown et al., 2011), although a significant portion of DCo (up to 15%) can be stabilized and transported as inorganic species in those DCo-enriched water-masses. Contrastingly, the distribution of L does not suggest an intense biological production of L around the Antarctic Polar Front where a diatom bloom had recently occurred. Here [Co'] can be several orders of magnitude higher than those reported in the subtropical domain, suggesting that cobalt limitation was unlikely in the ACC domain. The almost invariant L:DCo ratio of ~1 recorded in these surface waters also reflected the conservative behaviours of both L and DCo. In deeper waters higher ligand concentrations were observed in waters previously identified as DCo sources (Bown et al., 2011). At those depths the eastward increase of DCo from the Drake Passage to the Greenwich Meridian could be associated with a large scale transport and remineralisation of DCo as organic complexes; here, the fraction stabilized as inorganic Co was also significant (up to 25%) in the low oxygenated Upper Circumpolar Deep Waters. Organic speciation may thus be a central factor in the biogeochemical cycle of DCo in those areas, playing a major role in the bioavailability and the geochemistry of Co.
New insights on the role of organic speciation in the biogeochemical cycle of dissolved cobalt in the southeastern Atlantic and the Southern Ocean
J. Bown,M. Boye,D. M. Nelson
Biogeosciences Discussions , 2012, DOI: 10.5194/bgd-9-3381-2012
Abstract: The organic speciation of dissolved cobalt was investigated in the subtropical region of the southeastern Atlantic, and in the Antarctic Circumpolar Current (ACC) and the northern Weddell Gyre in the Southern Ocean between 33°58′S and 57°33′S along the Greenwich Meridian during the austral summer of 2008. The organic speciation of cobalt was determined by Competing Ligand Exchange Adsorptive Cathodic Stripping Voltammetry (CLE-AdCSV) using nioxime as a competing ligand. The conditional stability constants (log K'CoL) of the organic complexes of Co ranged between 17.9 and 20.1, and the concentrations of the organic ligands (L) between 26 and 73 pM. Most dissolved cobalt (DCo) was organically complexed in the water-column (60 to ≥99.9 %). There were clear vertical and meridional patterns in the distribution of L and the organic speciation of DCo along the section. These patterns suggested a biological source of the organic ligands in the surface waters of the subtropical domain and northern subantarctic region, potentially driven by the cyanobacteria, and a removal of the organic Co by direct or indirect biological uptake. The highest L:DCo ratio (e.g. 5.81 ± 1.07 pM pM–1) observed in these surface waters reflected the combined effects of ligand production and consumption of DCo. As a result of these combined effects, the calculated concentrations of free, unbound Co ([Co′]) in subtropical and subantarctic surface waters were very low, generally between 10–19 and 10–17 M. In intermediate and deep waters, the South African margins can be a source of organic ligands, as it was suggested to be for DCo (Bown et al., 2011), although a significant portion of DCo (up to 15 %) can be stabilized and transported as inorganic species in those DCo-enriched water-masses. Contrastingly, the distribution of L did not suggest an intense biological production of L around the Antarctic Polar Front where a diatom bloom had recently occurred. Here [Co′] can be several orders of magnitude higher than those reported in the subtropical domain, suggesting that cobalt limitation was unlikely in the ACC domain. The almost invariant L:DCo ratio of ~1 recorded in these surface waters also reflected the conservative behaviours of both the organic ligands and DCo. In deeper waters relatively higher ligand concentrations were observed in waters previously identified as DCo sources (Bown et al., 2011). At those depths the eastward increase of DCo could be associated with a large scale transport and remineralisation of DCo as organic complexes; here, the fraction stabilized as inorganic
Imprint of a dissolved cobalt basaltic source on the Kerguelen Plateau
J. Bown,M. Boye,P. Laan,A. R. Bowie
Biogeosciences Discussions , 2012, DOI: 10.5194/bgd-9-7291-2012
Abstract: Processes of cobalt (Co) entrainment from shelf sediments over the Kerguelen Plateau were studied during the KEOPS (Kerguelen Ocean Plateau compared Study) in order to explain the exceptionally high dissolved cobalt concentrations that have been measured in the surface waters above the Kerguelen Plateau, and in intermediate and deep waters above its eastern slope. Lateral advection and dissolution of Co contained in basalt sediments around Heard Island, a main source of lithogenic Co in the study area, were shown to imprint the process of surface enrichment over the plateau. Dissolved Co enrichment was strongest at the intercept of the eastern slope with intermediate and deep waters, probably due to more efficient mobilisation of the sediments in the slope current, in addition to advection of Co-enriched and low oxygenated ocean water-masses. In surface waters, strong sedimentary Co inputs were estimated to be much higher than biological Co uptake in phytoplankton blooms, underlining the potential role of cobalt as lithogenic tracer. Based on a simple, steady state balance equation of the external input of dissolved iron over the plateau, the fertilization of iron inferred by using Co as a tracer of basalt sources is estimated to be 28 × 102 ± 21 × 102 ton yr 1 in surface waters of the Kerguelen Plateau. This estimate is consistent with preceding ones and the required iron supply matching the phytoplankton demand.
Distributions of dissolved trace metals (Cd, Cu, Mn, Pb, Ag) in the southeastern Atlantic and the Southern Ocean
M. Boye,B. D. Wake,P. Lopez Garcia,J. Bown
Biogeosciences Discussions , 2012, DOI: 10.5194/bgd-9-3579-2012
Abstract: Comprehensive synoptic datasets (surface water down to 4000 m) of dissolved cadmium (Cd), copper (Cu), manganese (Mn), lead (Pb) and silver (Ag) are presented along a section between 34° S and 57° S in the southeastern Atlantic Ocean and the Southern Ocean to the south off South Africa. The vertical distributions of Cu, Ag, and of Cd display nutrient-like profiles similar to silicic acid, and phosphate, respectively. The distribution of Mn shows a subsurface maximum in the oxygen minimum zone, whereas Pb concentrations are rather invariable with depth. Dry deposition of aerosols is thought to be an important source of Pb to surface waters close to South Africa, and dry deposition and snowfall may have been significant sources of Cu and Mn at the higher latitudes. Furthermore, the advection of water-masses enriched in trace metals following contact with continental margins appeared to be an important source of trace elements to the surface, intermediate and deep waters in the southeastern Atlantic Ocean and the Antarctic Circumpolar Current. Hydrothermal inputs appeared to have formed a source of trace metals to the deep waters over the Bouvet Triple Junction ridge crest, as suggested by relatively enhanced dissolved Mn concentrations. The biological utilization of Cu and Ag was proportional to that of silicic acid across the section, suggesting that diatoms formed an important control over the removal of Cu and Ag from surface waters. However uptake by dino- and nano-flagelattes may have influenced the distribution of Cu and Ag in the surface waters of the subtropical Atlantic domain. Cadmium correlated strongly with phosphate (P), yielding lower Cd/P ratios in the subtropical surface waters where phosphate concentrations were below 0.95 μM. The greater depletion of Cd relative to P observed in the Weddell Gyre compared to the Antarctic Circumpolar Current could be due to increase Cd-uptake induced by iron-limiting conditions in these High-Nutrient Low-Chlorophyll waters. Similarly, an increase of Mn uptake under Fe-depleted conditions may have caused the highest depletion of Mn relative to P in surface waters of the Weddell Gyre. In addition, a cellular Mn-transport channel of Cd was possibly activated in the Weddell Gyre, which in turn may have yielded depletion of both Mn and Cd in these surface waters.
Are Tent Cities an Illusion of Success? Clients’ Perceptions of Neoliberalism Strategies  [PDF]
Francisco J. Alatorre
Open Journal of Social Sciences (JSS) , 2016, DOI: 10.4236/jss.2016.411010
Abstract:
The term, neoliberalism has been used to describe the overarching political and economic framework in which services for the poor living outside institutional life has operated for roughly the past 30 years. This implies a unified intent to “manage the poor” to benefit the larger society, in particular the business sector and the wealthy which are the key actors in society according to neoliberal doctrine. This paper explores and questions the conventional ideology of “managing the poor” and thus inquires further about the identified objectives of such management: whether such programs are intended to help the homeless re-establish themselves or prevent them from impinging on the wealthier classes. This questioning is done by reporting on and analyzing the experiences of twenty homeless people who are currently being “managed” by services provided by the agency Mesilla Valley Community of Hope (MVCH) in Las Cruces, New Mexico. This agency, reflecting Neoliberal doctrines, has created and manages a tent city which provides services for people who are homeless and near homeless. These services include showers, laundry, mail, voice mail, phone, lockers, case management, training programs, and donated goods, including clothing, shoes, hygiene products and household goods. MVCH also has various housing programs including transitional and permanent housing for people who are homeless. The findings derived from the interviewed participants reveal that “the managed” provide a contrasting view with those who assess neoliberalism practices as only benefiting society.
Hpz1 Modulates the G1-S Transition in Fission Yeast
Cathrine A. B?e, Jon Halvor J. Knutsen, Erik Boye, Beáta Grallert
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044539
Abstract: Here we characterize a novel protein in S. pombe. It has a high degree of homology with the Zn-finger domain of the human Poly(ADP-ribose) polymerase (PARP). Surprisingly, the gene for this protein is, in many fungi, fused with and in the same reading frame as that encoding Rad3, the homologue of the human ATR checkpoint protein. We name the protein Hpz1 (Homologue of PARP-type Zn-finger). Hpz1 does not possess PARP activity, but is important for resistance to ultraviolet light in the G1 phase and to treatment with hydroxyurea, a drug that arrests DNA replication forks in the S phase. However, we find no evidence of a checkpoint function of Hpz1. Furthermore, absence of Hpz1 results in an advancement of S-phase entry after a G1 arrest as well as earlier recovery from a hydroxyurea block. The hpz1 gene is expressed mainly in the G1 phase and Hpz1 is localized to the nucleus. We conclude that Hpz1 regulates the initiation of the S phase and may cooperate with Rad3 in this function.
An Unexpected Location of the Arginine Catabolic Mobile Element (ACME) in a USA300-Related MRSA Strain
Mette Damkj?r Bartels,Lars Hestbjerg Hansen,Kit Boye,S?ren J. S?rensen,Henrik Westh
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0016193
Abstract: In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially described in USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A common health-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCR positive for the ACME specific arcA-gene. This study is the first to describe an ACME element upstream of the SCCmec in MRSA. By traditional SCCmec typing schemes, the SCCmec of t024-ST8 strain M1 carries SCCmec IVa, but full sequencing of the cassette revealed that the entire J3 region had no homology to published SCCmec IVa. Within the J3 region of M1 was a 1705 bp sequence only similar to a sequence in S. haemolyticus strain JCSC1435 and 2941 bps with no homology found in GenBank. In addition to the usual direct repeats (DR) at each extremity of SCCmec, M1 had two new DR between the orfX gene and the J3 region of the SCCmec. The region between the orfX DR (DR1) and DR2 contained the ccrAB4 genes. An ACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highly similar to parts of the ACME composite island of S. epidermidis strain ATCC12228. Sequencing of an ACME negative t024-ST8 strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-like element inserted downstream of orfX and upstream of SCCmec indicates a novel recombination between staphylococcal species.
Distributions of dissolved trace metals (Cd, Cu, Mn, Pb, Ag) in the southeastern Atlantic and the Southern Ocean
M. Boye, B. D. Wake, P. Lopez Garcia, J. Bown, A. R. Baker,E. P. Achterberg
Biogeosciences (BG) & Discussions (BGD) , 2012,
Abstract: Comprehensive synoptic datasets (surface water down to 4000 m) of dissolved cadmium (Cd), copper (Cu), manganese (Mn), lead (Pb) and silver (Ag) are presented along a section between 34° S and 57° S in the southeastern Atlantic Ocean and the Southern Ocean to the south off South Africa. The vertical distributions of Cu and Ag display nutrient-like profiles similar to silicic acid, and of Cd similar to phosphate. The distribution of Mn shows a subsurface maximum in the oxygen minimum zone, whereas Pb concentrations are rather invariable with depth. Dry deposition of aerosols is thought to be an important source of Pb to surface waters close to South Africa, and dry deposition and snowfall may have been significant sources of Cu and Mn at the higher latitudes. Furthermore, the advection of water masses enriched in trace metals following contact with continental margins appeared to be an important source of trace elements to the surface, intermediate and deep waters in the southeastern Atlantic Ocean and the Antarctic Circumpolar Current. Hydrothermal inputs may have formed a source of trace metals to the deep waters over the Bouvet Triple Junction ridge crest, as suggested by relatively enhanced dissolved Mn concentrations. The biological utilization of Cu and Ag was proportional to that of silicic acid across the section, suggesting that diatoms formed an important control over the removal of Cu and Ag from surface waters. However, uptake by dino- and nano-flagellates may have influenced the distribution of Cu and Ag in the surface waters of the subtropical Atlantic domain. Cadmium correlated strongly with phosphate (P), yielding lower Cd / P ratios in the subtropical surface waters where phosphate concentrations were below 0.95 μM. The greater depletion of Cd relative to P observed in the Weddell Gyre compared to the Antarctic Circumpolar Current could be due to increase Cd uptake induced by iron-limiting conditions in these high-nutrient–low-chlorophyll waters. Similarly, an increase of Mn uptake under Fe-depleted conditions may have caused the highest depletion of Mn relative to P in the surface waters of the Weddell Gyre. In addition, a cellular Mn-transport channel of Cd was possibly activated in the Weddell Gyre, which in turn may have yielded depletion of both Mn and Cd in these surface waters.
Rapid, widespread transduction of the murine myocardium using self-complementary Adeno-associated virus
Andino Lourdes M,Conlon Thomas J,Porvasnik Stacy L,Boye Sanford L
Genetic Vaccines and Therapy , 2007, DOI: 10.1186/1479-0556-5-13
Abstract: Adeno-associated virus (AAV) has shown great promise as a gene transfer vector. However, the incubation time needed to attain significant levels of gene expression is often too long for some clinical applications. Self-complementary AAV (scAAV) enters the cell as double stranded DNA, eliminating the step of second-strand synthesis, proven to be the rate-limiting step for gene expression of single-stranded AAV (ssAAV). The aim of this study was to compare the efficiency of these two types of AAV vectors in the murine myocardium. Four day old CD-1 mice were injected with either of the two AAV constructs, both expressing GFP and packaged into the AAV1 capsid. The animals were held for 4, 6, 11 or 21 days, after which they were euthanized and their hearts were excised. Serial sections of the myocardial tissue were used for real-time PCR quantification of AAV genome copies and for confocal microscopy. Although we observed similar numbers of AAV genomes at each of the different time points present in both the scAAV and the ssAAV infected hearts, microscopic analysis showed expression of GFP as early as 4 days in animals injected with the scAAV, while little or no expression was observed with the ssAAV constructs until day 11. AAV transduction of murine myocardium is therefore significantly enhanced using scAAV constructs.
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