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Search Results: 1 - 10 of 3011 matches for " Florian Willecke "
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Successful Therapy of Ventricular Rupture by Percutaneous Intrapericardial Instillation of Fibrin Glue: A Case Report
Florian Willecke,Christoph Bode,Andreas Zirlik
Case Reports in Vascular Medicine , 2013, DOI: 10.1155/2013/412341
Abstract: Rupture of the ventricular myocardium is an often lethal complication after myocardial infarction. Due to the dramatic hemodynamics and the short time frame between ventricular rupture and surgical closure of the defect, additional therapeutic strategies are needed. Here we report the successful therapy of ventricular rupture by percutaneous intrapericardial instillation of fibrin glue in a 72-year-old male patient with postinfarct angina secondary to anterior myocardial infarction. 1. Case Report Rupture of the ventricular myocardium after myocardial infarction is a dramatic and often lethal complication. Due to the dramatic hemodynamic dysfunction, immediate therapies are imperative. As surgical repair of the defect is often not available, percutaneous intrapericardial instillation of fibrin glue can be an alternative. A 72-year-old male patient with postinfarct angina secondary to anterior myocardial infarction was transferred to our center from a community hospital after administration of systemic thrombolytic therapy using streptokinase. Coronary angiography showed single vessel disease with high grade stenosis of the LAD. Stent implantation was successfully performed with uncomplicated postinterventional course. On day three, the patient developed another episode of angina. Recatheterization excluded acute restenosis or stent thrombosis. On the same day, the patient developed rapid onset cardiogenic shock with need for resuscitation, intubation, high dose catecholamine treatment, and an intra-aortic balloon pump. Echocardiography showed an acute pericardial tamponade suggesting a ventricular rupture (Figure 1(a)). Pericardiocentesis was performed, and large amounts of blood could be aspirated and were directly retransfused. Hemodynamics stabilised only under constant aspiration. As ultima ratio, we instillated a total of 30?mL of a two-component fibrin glue normally used for bleeding ulcers in gastroenterology. This resulted in a sustained hemodynamic stabilization. The patient could be weaned off the balloon pump and catecholamines in the following three days. Echocardiography showed a stable minor pericardial effusion of 100?mL without any signs of hemodynamic relevance (Figure 1(b)). Unfortunately, on day nine, the patient gradually developed signs of progressive cardiogenic shock again with the need of cathecolamine treatment and finally died from pump failure on day 13. Serial echocardiographic evaluations were negative for relevant pericardial effusion. Autopsy revealed a fibrin glue induced focal peri-epicardial adhesion and extensive
CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies
Dennis Wolf, Felix Jehle, Alexandra Ortiz Rodriguez, Bianca Dufner, Natalie Hoppe, Christian Colberg, Andrey Lozhkin, Nicole Bassler, Benjamin Rupprecht, Ansgar Wiedemann, Ingo Hilgendorf, Peter Stachon, Florian Willecke, Mark Febbraio, Christoph J. Binder, Christoph Bode, Andreas Zirlik, Karlheinz Peter
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033026
Abstract: Background Adipose tissue inflammation fuels the metabolic syndrome. We recently reported that CD40L – an established marker and mediator of cardiovascular disease – induces inflammatory cytokine production in adipose cells in vitro. Here, we tested the hypothesis that CD40L deficiency modulates adipose tissue inflammation in vivo. Methodology/Principal Findings WT or CD40L?/? mice consumed a high fat diet (HFD) for 20 weeks. Inflammatory cell recruitment was impaired in mice lacking CD40L as shown by a decrease of adipose tissue macrophages, B-cells, and an increase in protective T-regulatory cells. Mechanistically, CD40L-deficient mice expressed significantly lower levels of the pro-inflammatory chemokine MCP-1 both, locally in adipose tissue and systemically in plasma. Moreover, levels of pro-inflammatory IgG-antibodies against oxidized lipids were reduced in CD40L?/? mice. Also, circulating low-density lipoproteins and insulin levels were lower in CD40L?/? mice. However, CD40L?/? mice consuming HFD were not protected from the onset of diet-induced obesity (DIO), insulin resistance, and hepatic steatosis, suggesting that CD40L selectively limits the inflammatory features of diet-induced obesity rather than its metabolic phenotype. Interestingly, CD40L?/? mice consuming a low fat diet (LFD) showed both, a favorable inflammatory and metabolic phenotype characterized by diminished weight gain, improved insulin tolerance, and attenuated plasma adipokine levels. Conclusion We present the novel finding that CD40L deficiency limits adipose tissue inflammation in vivo. These findings identify CD40L as a potential mediator at the interface of cardiovascular and metabolic disease.
Cannabinoid Receptor 2 Signaling Does Not Modulate Atherogenesis in Mice
Florian Willecke,Katharina Zeschky,Alexandra Ortiz Rodriguez,Christian Colberg,Volker Auw?rter,Stefan Kneisel,Melanie Hutter,Andrey Lozhkin,Natalie Hoppe,Dennis Wolf,Constantin von zur Mühlen,Martin Moser,Ingo Hilgendorf,Christoph Bode,Andreas Zirlik
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019405
Abstract: Strong evidence supports a protective role of the cannabinoid receptor 2 (CB2) in inflammation and atherosclerosis. However, direct proof of its involvement in lesion formation is lacking. Therefore, the present study aimed to characterize the role of the CB2 receptor in Murine atherogenesis.
Expression of connexin genes in the human retina
Goran S?hl, Antonia Joussen, Norbert Kociok, Klaus Willecke
BMC Ophthalmology , 2010, DOI: 10.1186/1471-2415-10-27
Abstract: Northern blot hybridizations, RT-PCR as well as immunofluorescence analyses helped to explore at least partially the expression pattern of the following human connexin genes GJD2 (hCx36), GJC1 (hCx45), GJA9 (hCx59) and GJA10 (hCx62) in the human retina.Here we report that Northern blot hybridization signals of the orthologuous hCx36 and hCx45 were found in human retinal RNA. Immunofluorescence signals for both connexins could be located in both inner and outer plexiform layer (IPL, OPL). Expression of a third connexin gene denoted as GJA10 (Cx62) was also detected after Northern blot hybridization in the human retina. Interestingly, its gene structure is similar to that of Gja10 (mCx57) being expressed in mouse horizontal cells. RT-PCR analysis suggested that an additional exon of about 25 kb further downstream, coding for 12 amino acid residues, is spliced to the nearly complete reading frame on exon2 of GJA10 (Cx62). Cx59 mRNA, however, with high sequence identity to zebrafish Cx55.5 was only weakly detectable by RT-PCR in cDNA of human retina.In contrast to the neuron-expressed connexin genes Gjd2 coding for mCx36, Gjc1 coding for mCx45 and Gja10 coding for mCx57 in the mouse, a subset of 4 connexin genes, including the unique GJA9 (Cx59) and GJA10 (Cx62), could be detected at least as transcript isoforms in the human retina. First immunofluorescence analyses revealed a staining pattern of hCx36 and hCx45 expression both in the IPL and OPL, partially reminiscent to that in the mouse, although additional post-mortem material is needed to further explore their sublamina-specific distribution. Appropriate antibodies against Cx59 and Cx62 protein will clarify expression of these proteins in future studies.Gap junction channels allow direct metabolic and electrical communication between neighboring cells. They are composed of two hemi-channels also denoted as connexons, each constisting of six connexin protein subunits [1]. Gap Junctions are widely distributed among m
Cell-Sorting at the A/P Boundary in the Drosophila Wing Primordium: A Computational Model to Consolidate Observed Non-Local Effects of Hh Signaling
Sabine Schilling,Maria Willecke,Tinri Aegerter-Wilmsen,Olaf A. Cirpka,Konrad Basler,Christian von Mering
PLOS Computational Biology , 2011, DOI: 10.1371/journal.pcbi.1002025
Abstract: Non-intermingling, adjacent populations of cells define compartment boundaries; such boundaries are often essential for the positioning and the maintenance of tissue-organizers during growth. In the developing wing primordium of Drosophila melanogaster, signaling by the secreted protein Hedgehog (Hh) is required for compartment boundary maintenance. However, the precise mechanism of Hh input remains poorly understood. Here, we combine experimental observations of perturbed Hh signaling with computer simulations of cellular behavior, and connect physical properties of cells to their Hh signaling status. We find that experimental disruption of Hh signaling has observable effects on cell sorting surprisingly far from the compartment boundary, which is in contrast to a previous model that confines Hh influence to the compartment boundary itself. We have recapitulated our experimental observations by simulations of Hh diffusion and transduction coupled to mechanical tension along cell-to-cell contact surfaces. Intriguingly, the best results were obtained under the assumption that Hh signaling cannot alter the overall tension force of the cell, but will merely re-distribute it locally inside the cell, relative to the signaling status of neighboring cells. Our results suggest a scenario in which homotypic interactions of a putative Hh target molecule at the cell surface are converted into a mechanical force. Such a scenario could explain why the mechanical output of Hh signaling appears to be confined to the compartment boundary, despite the longer range of the Hh molecule itself. Our study is the first to couple a cellular vertex model describing mechanical properties of cells in a growing tissue, to an explicit model of an entire signaling pathway, including a freely diffusible component. We discuss potential applications and challenges of such an approach.
A Liquid Chromatography Assay for the Simultaneous Quantification of Piperacillin and Ciprofloxacin in Human Plasma and Dialysate in Critically Ill Patients Undergoing Continuous Renal Replacement Therapy  [PDF]
Florian Scheer, Irene Kr?mer
International Journal of Analytical Mass Spectrometry and Chromatography (IJAMSC) , 2014, DOI: 10.4236/ijamsc.2014.22005

Piperacillin/tazobactam and ciprofloxacin are often used in combination as initial empiric anti-biotic therapy in critical ill patients. Especially in patients undergoing continuous renal replacement therapy (CRRT) the pharmacokinetics of antimicrobial agents can be highly variable. In order to avoid under- or overdosage of antibiotics therapeutic drug monitoring (TDM) is highly re-commendable. Based on two known HPLC assays for piperacillin a new method in combination with solid phase extraction (SPE) for the simultaneous determination of piperacillin and ciprofloxacin was developed. Method validation was performed according to the EMA guideline on validation of bioanalytical methods. The HPLC column used was a Perfect Bond ODS-HD C18 analytical column (100 mm × 4.6 mm i.d., particle size 5 μm), equipped with a guard column (10 mm × 4.6 mm, particle size 5 μm) containing the same packing material. Detection wavelength was set at 228 nm for piperacillin and benzylpenicillin was used as internal standard (IS). Ciprofloxacin was determined at two wavelengths (280 nm, 315 nm). This newly developed HPLC method in combination with SPE-extraction allows an accurate, precise, specific and efficient determination of piperacillin and ciprofloxacin in biological matrices. Results allow the calculation of all relevant pharmacokinetic data for critically ill patients undergoing CRRT and the optimization of dosing and TDM.

On the equation
Florian Luca
International Journal of Mathematics and Mathematical Sciences , 2002, DOI: 10.1155/s0161171202004696
Abstract: We find all positive integer solutions (x,y,a,b,n) of x2
How to Understand the Cell by Breaking It: Network Analysis of Gene Perturbation Screens
Florian Markowetz
PLOS Computational Biology , 2010, DOI: 10.1371/journal.pcbi.1000655
A Novel Platform for Research on Toxins
Florian Lang
Toxins , 2009, DOI: 10.3390/toxins1010001
Abstract: Toxins are ubiquitous in nature and as such they impact our daily life. Toxins may come from a wide variety of sources and influence a myriad of biological functions. Research on toxins may address their production, structure, chemical properties, biological activity, and economic impact. On the one hand, toxins may be used to decipher biological mechanisms, to favourably influence disease or to combat unwanted organisms. On the other hand, toxins may be a hazard jeopardizing health of humans, animals or plants. [...]
An application of kernel methods to variety identification based on SSR markers genetic fingerprinting
Florian Martin
BMC Bioinformatics , 2011, DOI: 10.1186/1471-2105-12-177
Abstract: An approach encoding SSR polymorphisms in a positive definite kernel is presented, which then allows the usage of any kernel supervised method. The polymorphism between the samples is encoded through the Nei-Li genetic distance, which is shown to define a positive definite kernel between the genotyped samples. Additionally, a greedy feature selection algorithm for selecting SSR marker kits is presented to build economical and efficient prediction models for discrimination. The algorithm is a filter method and outperforms other filter methods adapted to this setting. When combined with kernel linear discriminant analysis or kernel principal component analysis followed by linear discriminant analysis, the approach leads to very satisfactory prediction models.The main advantage of the approach is to benefit from a flexible way to encode polymorphisms in a kernel and when combined with a feature selection algorithm resulting in a few specific markers, it leads to accurate and economical identification models based on SSR genotyping.Genetic markers are target sites in the genome that differ between individuals of a population. These differences can occur in DNA that codes for specific genes, or usually in the vast areas of intergenic DNA. These differences in the make-up of the genetic content at a specific site in the genome are often referred to as polymorphisms (literally "multiple forms"). These polymorphisms are detected with a range of different technologies of which simple sequence repeat markers (SSRs) [1] and single nucleotide polymorphisms (SNPs) are currently the most commonly used types. The markers used in this study are SSRs. The SSRs of interest for marker development include di-nucleotide and higher order repeats (e.g. (AG)n, (TAT )n, etc.). The number of repeats usually ranges between just a few units to several dozens of units. The polymorphism can exist at a locus containing a microsatellite between individuals of a population and is characterized as a
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