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Search Results: 1 - 10 of 218717 matches for " Fernando Esperón "
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Antimicrobial Resistance Genes in Animal Manure, Manure-Amended and Nonanthropogenically Impacted Soils in Spain  [PDF]
Fernando Esperón, Carlos Sacristán, Matilde Carballo, Ana de la Torre
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.99032
Abstract: Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended [n = 8] and nonmanure-amended [n = 8]). All samples were collected in central Spain. Detection was based on 18 selected antimicrobial resistance genes (ARGs). The most commonly detected genes in animal manure were sul1 (16/16), sul2 (16/16), tet(A) (16/16), aadA (16/16), tet(B) (15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8) were more frequently detected in chicken manure, whereas pig slurry samples presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the four genes selected for their clinical relevance, three—blaCTX-M
Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain  [PDF]
Matilde Carballo, Fernando Esperón, Carlos Sacristán, Miguel González, Belén Vázquez, Sonia Aguayo, Ana de la Torre
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.42A040
Abstract:
The usage of antibiotics in animal husbandry has dramatically increased the concentration of antibiotic residues and has promoted the development and abundance of antibiotic resistance in manure. When it is spread onto agricultural land, both residues of antibiotics and bacteria carrying antibiotic resistance genes may be introduced into the environment. In this research, we isolated resistant gram negative bacteria from manure produced in two dairy and two beef cattle farms, located in Madrid (Spain), to determine their resistance to seventeen representative antibiotics commonly used in veterinary therapy. A total of 63 isolates were used to assess the overall bacterial antimicrobial resistance on cattle manure samples. Predominant species were Escherichia coli and Comamonas testosteroni accounting for 25% and 19.6% of the total, respectively. The most found antimicrobial resistances in gram-negative bacteria were to tetracycline (66.7%), sulphamethoxazole (55.6%), ampicillin (52.4%), cephalothin (46.0%), chloramphenicol (44.4%), nalidixic acid (39.7%) and trimethroprim- sulphamethoxazole (33.3%). The mean of resistance and the percentage of multi-resistant bacteria in beef farms were higher and statistically significant when compared to dairy farms which is opposite from the findings of the previous studies. The presence of three tetracyclines in all manure samples was also examined with stable recoveries (76% - 82%) and high sensitivity (limit of quantification 0.015 - 0.03 μg/kg). The concentrations of tetracyclines detected (<0.015 - 10 mg/kg) were consistent to the theoretical tetracycline levels in manure in Spain according to the ex- cretion rate of these antibiotics and the values re-ported in scientific literature in other European coun- tries.
Screening for several potential pathogens in feral pigeons (Columba livia) in Madrid
Belén Vázquez, Fernando Esperón, Elena Neves, Juan López, Carlos Ballesteros, María Mu?oz
Acta Veterinaria Scandinavica , 2010, DOI: 10.1186/1751-0147-52-45
Abstract: A total of 118 pigeons were captured in three samplings carried out in 2006-2007 in public parks and gardens in Madrid, Spain. Standard haematological and morphological analyses were carried out on the pigeons. PCR was used to screen for the presence of Campylobacter jejuni, C. coli and Chlamydophila psittaci. Positive samples were confirmed by DNA sequencing.The analyses demonstrated a high prevalence of Chlamydophila psittaci (52.6%) and Campylobacter jejuni (69.1%) among the birds captured. In contrast, Campylobacter coli was rarely detected (1.1%).Pigeons in Madrid can carry Chlamydophila psittaci and Campylobacter jejuni. They may be asymptomatic or subclinical carriers of both pathogens.Public parks and gardens are home to abundant populations of birds. One of the most frequent species is the feral pigeon (Columba livia), which can be present at densities higher than 2,000 individuals per km2, as in Milan [1] or Barcelona [2]. Unfortunately no data are available about pigeon densities in many other major cities, such as Madrid.Although there are few reports of disease transmission between pigeons and humans [3], their close interaction, together with the observation that these birds are vectors for zoonotic agents [4], may make them a public health risk. In addition, recent work showed that pigeons can cover a maximum distance of 5.29 km [5]; thus, they can spread pathogens locally in their environment.Thermophilic Campylobacter species, especially Campylobacter jejuni and Campylobacter coli, are considered the primary pathogens responsible for acute diarrhea in the world [6]. In fact, in several countries - e.g. England and Wales, Canada, Australia and New Zealand - Campylobacter jejuni infection causes more cases of acute diarrhea annually than do Salmonella spp. [7]. In Spain an average of 3,500 cases of C. jejuni infection per year has been reported for the period 1989-2001 [7].In the US, as many as 15% of Campylobacter spp. infections may be attributable
Herpes virus infection associated with interstitial nephritis in a beaked whale (Mesoplodon densirostris)
Manuel Arbelo, Edwige N Bellière, Eva Sierra, Simona Sacchinni, Fernando Esperón, Marisa Andrada, Miguel Rivero, Josue Diaz-Delgado, Antonio Fernández
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-243
Abstract: On April 2004, an old adult male Blainville’s beaked whale (Mesoplodon densirostris) 420 cm long with a poor body condition was stranded on Tenerife Island. During necropsy, no gross lesions were observed in the kidneys. However, membranous glomerulonephritis, multifocal interstitial lymphoplasmacytic nephritis and acute multifocal necrotizing tubulointerstitial nephritis with intranuclear inclusion bodies was diagnosed by histological analysis. Tissue samples were submitted for bacteriological analysis and molecular viral screening.A novel alpha herpesvirus associated with interstitial nephritis was identified in an old adult male Blainville's beaked whale (M. densirostris) with a poor body condition stranded in the Canary Islands. This report suggests that identification of herpesvirus infection could be used as a differential diagnosis for interstitial nephritis in cetaceans.The presence of herpesviruses (HV) in cetaceans was shown in the late 1980s by electron microscopy (EM) analysis demonstrating HV-like particles in skin biopsies from beluga whales (Delphinapterus leucas) [1,2]. EM analysis also demonstrated that HV was associated with encephalitis in a harbor porpoise (Phocoena phocoena) [3] and with skin lesions of dusky dolphins (Lagenorhynchus obscures) [4]. In addition to EM, immunoperoxidase staining [3], serum neutralization and enzyme-labeled immunosorbent assays have been used as indicators for the presence of HV in cetaceans [5]. Alpha-HV have been associated with fatal systemic infections in bottlenose dolphins (Tursiops truncatus) [6], a Cuvier’s beaked whale (Ziphius cavirostris) [7], and with cutaneous lesions in bottlenose dolphins [8]. Gamma-HV have been identified in mucosal lesions in bottlenose dolphins, Risso’s dolphins (Grampus griseus), dwarf sperm whales (Kogia sima) and Blainville’s beaked whales (Mesoplodon densirostris) [8-10].The capacity of HV to cause disease in cetaceans is unclear and may show variations depending on the differe
Metodología para definir funciones profesionales
Torres Esperón,Maricela;
Revista Cubana de Salud Pública , 2008, DOI: 10.1590/S0864-34662008000400017
Abstract: a methodology for definition of professional functions, designed and validated by the author in a national research work of the branch research program on health systems and services in cuba performed from 2002 to 2004, was used. the results of the study have been used for the regulation of nursing practice in 2008. this methodology is structured in three phases in which use of qualitative techniques such as literature review, expert consultation, in this case to two different groups, one as expert committee and the other as expert panel for the implementation of delphi method, and finally, the observational technique for verification in practice. a survey of the two groups of experts is administered in the intermediate process. the dialectical and participatory nature of the submitted methodology makes it possible that each phase be enhanced by the other phase and thus a scientifically proven product is achieved.
Memorias y perspectivas de la I Reunión de Redes de Enfermería en Cuba
Torres Esperón,Maricela;
Revista Cubana de Enfermer?-a , 2011,
Abstract: the scientific-technical development of nursing staff needs of collaborative construction to strength the care process, teaching and research. nowadays, the networks are the more effective route for it, thus if these are conceived as a source of information, exchange and communication, based on the tolerance, respect and solidarity, will become a science. to make the most of the chances from nursing congresses to carry out networks meetings is an alternative to motivate and to strength the scientific and human exchange, this is the case of the 1st networks meeting had in the xiv congress of the cuban nursing society. the aim of present paper is to describe the memories of this successful meeting and to give shape to the perspectives serving of guide for the future work of the nursing networks in cuba over 2011-2013.
V Reunión Internacional de Organización de Redes de Enfermería. Por la educación hacia el logro de los Objetivos del Milenio
Torres Esperón,Maricela;
Revista Cubana de Enfermer?-a , 2011,
Abstract: the international networks of nursing are aimed to improve the education, the care practice and the research depending on the person's health. the initiative has important backgrounds as regards the network strategy in the pan-american nursing environment and to collect and make operational the guidelines of the paho concerning the coordination of knowledges networks. the collaboration work of these networks started in the 2006 allowed to develop already the v international meeting of nursing networks organization held in september, 201l. in present paper are showed some notes related to this fifth meeting where guiding were approached and recommended to transform of nursing education in each subject matter area towards the achievement of the millennium objectives and the development of health systems based on the primary health care.
El trabajo en redes en la práctica de enfermería:: "Acortando la distancia"
Torres Esperón,Maricela;
Revista Cubana de Enfermer?-a , 2010,
Abstract: the networking is a strategy that links the linkages and exchanged between institutions and/or people who decide to involve voluntary and concerted efforts, experience and expertise to achieve common goals. the network is the result of that strategy and is an organizational and management mode, whose key features are: adaptability, regulatory flexibility, openness, horizontality, fluency and spontaneity of relationships. the incorporation of nursing to this modality has not been for no reason but has responded to factors that have predisposed including the growing dispersion and transience of information, the advancement of technology in communications systems and the need ¨ bridge the gap¨ between the growing number of professionals in the field of research for the foundation of care. this paper aims to clarify some elements related to the manner of articulation of networking and its contribution to the development of nursing.
IV Reunión Internacional de Organización de Redes de Enfermería: un paso más por la integración para la salud
Torres Esperón,Maricela;
Revista Cubana de Enfermer?-a , 2010,
Abstract: the network work is an alternative more and more used for the integration and the development, in the nursing case there is a ongoing work by regions and continents and it has been spreading until to achieve an international overview, such network meeting that initially were called pan-americans, in the 2010 the iv international meeting of nursing networks organization is carried out. in present paper are showed some notes of this fourth nursing networks meeting that also to propose techniques and methodological guiding for the development emphasizing in systematization of good nursing practices allowed to share experiences in specific area of each network, as well as to define researches priorities and to reconstruct the work plans and all this was made in communication, solidarity and affection environment.
Experiencias de la Red de Enfermería en Salud Infantil Cubana Experiences of Nursing Network in Cuban children health
Maricela Torres Esperón
Revista Cubana de Enfermer?-a , 2010,
Abstract:
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