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Search Results: 1 - 10 of 6690 matches for " Fernanda Monego "
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OCORRêNCIA DE BOTULISMO EM BOVINOS CONFINADOS NO RIO GRANDE DO SUL
Franciele Maboni,Fernanda Monego,Iveraldo Dutra,Mateus Matiuzzi da Costa
Ciência Animal Brasileira , 2010,
Abstract: Botulism is the intoxication caused by a neurotoxin produced by the Clostridium botulinum, distinguished by flacid or complete skeletal musculature paralysis. This study reports a botulism outbrake in feedlot bovine, fed with corn silage. Samples of the liver, and of the intestinal and ruminal content from one of the bovine that presented typical clinical state for botulism, as well as a portion of corn silage were sent for bacterial analysis. In the laboratory, bioassay and serum neutralization were performed on rats, confirming the suspect for type C botulism. This report intends to warn veterinaries and breeders, which practice herd feedlot, because this procedure increases botulism susceptibility, and it is mainly related to the type and quality of the food ingested.
Surto de listeriose sistêmica em chinchilas
Kirinus, Jackeline Karsten;Krewer, Carina;Zeni, Diego;Monego, Fernanda;Silva, Marcia Cristina da;Kommers, Glaucia Denise;Vargas, Agueda Castagna de;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000300033
Abstract: listeriosis is an infectious disease, which affects a variety of animal species and cause septicemia, encephalitis and abortion. chinchillas are the most susceptible animals to the systemic infection by listeria monocytogenes. this report describes an outbreak of systemic listeriosis in a farm of chinchillas in the central region of the rio grande do sul state, brazil. mortality rate was about 16%. on necropsy, there were multiple random small white foci on the liver capsule and parenchyma and enlargement of the hepatic lymph node. histologically, there were multifocal necro-suppurative hepatitis and suppurative lymphadenitis with numerous intralesional bacilli. l. monocytogenes was the etiology of the systemic listeriosis outbreak. the diagnosis was based on gross and microscopic lesions, genotypical and phenotypical characteristics and by immunohistochemistry technique.
Epidemiologic pattern of patients with neurocysticercosis diagnosed by computed tomography in Curitiba, Brazil
Grazziotin, Ana Laura;Fontalvo, Mariana Campos;Santos, Marilis Beckert Feijó;Monego, Fernanda;Grazziotin, Ana Line;Kolinski, Victor Hugo Zanini;Bordignon, Rodrigo Henrique;Biondo, Alexander Welker;Antoniuk, Affonso;
Arquivos de Neuro-Psiquiatria , 2010, DOI: 10.1590/S0004-282X2010000200022
Abstract: the aim of this study was to drawn an epidemiological pattern of neurocystisticercosis (ncc) patients diagnosed by computed tomography at the major private diagnostic center in curitiba, brazil. a total of 1,009 medical files of consecutive patients presenting neurological indications were diagnosed by computed tomography from july 2007 to april 2008. patient data included sex, age, municipality and tomography findings were analysed by epi-info version 6.0.1. software. most patients (81.10%) were living in curitiba. a total of 91/1,009 cases (9.02%) were considered positive to ncc; 88 (96.7%) patients had inactive form of ncc and only 3 (3.2%) patients had cysts in granulomatous process. no patients had both forms. the prevalence of ncc cases in studied group was greater in patients between 51 to 60 years old, however, difference between sex was not significant. this epidemiological pattern of ncc was similar to the first ncc study in curitiba, performed in 1995-1996 with 9.24% of positive cases.
Molecular identification and typing of Mycobacterium massiliense isolated from postsurgical infections in Brazil
Monego, Fernanda;Duarte, Rafael Silva;Nakatani, Sueli Massumi;Araújo, Wildo Navegantes;Riediger, Irina Nastassja;Brockelt, Sonia;Souza, Verena;Cataldo, Jamyra Iglesias;Dias, Rubens Clayton da Silva;Biondo, Alexander Welker;
Brazilian Journal of Infectious Diseases , 2011, DOI: 10.1590/S1413-86702011000500004
Abstract: objective: one hundred thirty-one cases of postsurgical infections were reported in southern region of brazil between august 2007 and january 2008. thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak. methods: all 39 isolates were analyzed by ziehl-nielsen stained smear, bacterial culture and submitted to rpob partial gene sequencing for identification. the isolates were also evaluated for their susceptibility to amikacin, cefoxitin, clarithromycin, ciprofloxacin, doxycycline, tobramycin and sulfamethoxazole. results: thirty-six isolates out of the confirmed cases were identified as mycobacterium massilienseand the remaining three were identified as mycobacterium abscessus, mycobacterium chelonae and mycobacterium fortuitum. all m. massiliense isolates were susceptible to amikacin (mic90 = 8 μg/ml) and clarithromycin (mic90 = 0.25 μg/ml) but resistant to cefoxitin, ciprofloxacin, doxycycline, tobramycin and sulfamethoxazole. molecular analysis by pulsed-field gel electrophoresis clustered all 36 m. massiliense isolates and showed the same pattern (bra 100) observed in three other outbreaks previously reported in brazil. conclusions: these findings suggest a common source of infection for all patients and reinforce the hypotheses of spread of m. massiliense bra100 in brazilian hospital surgical environment in recent years.
Determinantes de risco para doen?as cardiovasculares em escolares
Monego, Estelamaris T.;Jardim, Paulo César Brand?o Veiga;
Arquivos Brasileiros de Cardiologia , 2006, DOI: 10.1590/S0066-782X2006001400006
Abstract: objective: to investigate the occurrence and association of arterial hypertension with several lifestyle variables. methods: transversal population-based study with a random sample of students (7 to 14 years of age) of public and private schools. variables investigated were nutritional status, blood pressure, and lifestyle (tobacco use, alcohol intake, physical activity and eating habits). results: out of the 3,169 schoolchildren assessed, 5.0% had arterial hypertension and 6.2% had normal-high blood pressure. classification by gender shows boys 6.4% and girls 6.0% with normal-high blood pressure, and boys 4.3% and girls 5.7% with arterial hypertension. body mass index (bmi) measurements identified 16.0% excess weight students, 4.9% of whom were obese. a significant association (p = 0.01) between arterial hypertension and excess weight was observed. among the students participating in the study, 11.6% did not attend physical education classes and 37.8% had sedentary leisure habits. twenty students (0.6%) were smokers and 32.7% had already experimented with alcohol. none of these variables showed statistical significance as to blood pressure values and nutritional status. conclusion: in light of the findings in this study which show schoolchildren with a higher than expected frequency of mean blood pressure and bmi values, associated with a lifestyle that tends to favour the development of cardiovascular diseases, we felt led to propose interventional measures focused on the school as an agent of change and capable of conveying information to family units. this possibility encourages us to propose that schools be partners in promoting health.
Transcriptome of pig muscle assessed by erial analysis of gene expression (SAGE)
S. Dal Monego,A. Pallavicini,G. Graziosi,B. Stefanon
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2005.2s.88
Abstract: Serial analysis of gene expression (SAGE) is a molecular biology technique applied to measure the global gene expression levels, characterise transcriptomes, compare the transcript levels between tissues and uncover new molecules within defined signal transduction pathways (Tutela and Tuteja, 2004).
Evaluation of gene expression profiles of pig skeletal muscle in response to energy content of the diets using human microarrays
Simeone Dal Monego,Monica Colitti,Alberto Pallavicini,Mariasilvia D'Andrea
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.45
Abstract: The aim of the research was to compare gene transcription profiles of Musculus longissimus dorsi (MLD) between pigs fed diets with high (HED) or low (LED) energy contents. Two groups of 4 Casertana pigs were reared from 3 to 12 months of age in the same environmental conditions and fed HED or LED. In the HED, the ave rage daily gain and back fat thickness were significantly (P<0.05) higher than in LED pigs. Differential expression of genes in MLD of pigs fed diets with different energy density was assessed by a human high-density complementary DNA (cDNA) muscle microarray consisting of 4670 probes and further confirmed by quantitative real time RT-PCR analysis. Seven of the genes up-regulated in MLD of HED pigs were invo l ved in the glycolytic and ox i d a t i ve metabolism (phosphoglycerate mutase 2, glyc e ra l d e hyde-3-phosphate dehydrogenase, NADH dehydrogenase ubiquinone1 beta 9, muscle pyruvate kinase, enolase 3, muscle creatine kinase, isocitrate dehydrogenase 3 (NAD+) gamma) and four in the contractile apparatus (tropomyosin 1 alpha, troponin C2, fast, fast skeletal myosin light chain 2, troponin T3, skeletal, fast). Instead, HED diet reduced the level of expression of muscle proteins associated with slow fibre type (troponin T1, skeletal, slow, supervillin, myosin binding protein C, slow type, titin, myosin, heavy polypeptide 7, beta, calponin homology-associated smooth muscle) and signal transduction (SH3-binding domain protein 5-like, hypothetical protein FLJ21438, protein kinase cAMP-dependent, catalytic, rho guanine nucleotide exchange factor 15). The down-regulation of CTSB was also observed for HED group. From the results it can be assumed that high energy content of the diet influence physiological processes in the muscle tissue by switching slow fibres into fast reacting fibres and thus enhancing meat quality.
Serial Analysis of Gene Expression (SAGE) in the Skeletal Muscle of Pig
Simeone Dal Monego,Alberto Pallavicini,Giorgio Graziosi,Bruno Stefanon
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2009.417
Abstract: Skeletal muscle growth represents one of the main economic traits in pig production. To gain a better understanding of expressions profile in pig muscle, serial analysis of gene expression (SAGE) was performed in Longissimus dorsi of two pigs at 3 and 9 months of age. A total of 53,120 long tags were obtained and sequenced from the four muscle SAGE libraries, representing 17,902 different tags, or putative transcripts, 0.64% (+0.09) of which had a relative expression level higher than 1‰. Overall, a total of 218 tags were highly expressed and 31 had a frequency higher than 3‰. Functional characterisation of the expression profiles was performed using Kyoto Encyclopedia of Genes and Genomes metabolic maps and 139 pathways were identified for swine skeletal muscle. Focal adhesion, Mitogen-Activated Protein Kinase signalling, oxidative phosphorylation, ribosomal proteins, regulation of actin cytoskeleton and insulin signalling pathways showed an abundance of genes greater than 1.5% of all the expressed transcripts. A comparison with human SAGE library indicated no statistical differences for the frequency of genes involved in these metabolic pathways.
Evolution of transcriptome profiles during muscle development in Casertana and cosmopolite pig breeds
Mariasilvia D'Andrea,Alberto Pallavicini,Simeone Dal Monego,Renè Dreos
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2009.s2.66
Abstract: In order to determine candidate genes involved in production traits, the mRNA levels of muscle tissue of extremely different pig breeds (genotypes) were studied using microarray tool. Casertana, an autochthonous breed characterized by slow growth and a massive accumulation of backfat, was compared to Large White and to crossbred Duroc x Landrace x Large White)pigs. The differential expression of muscle genes was evaluated on 3 pigs for each genetic type using a microarray consisting of 10,665 oligos. Animals were of the same age and raised in the same environmental conditions. Muscle tissues were collected at 3, 6, 9, and 11 months and a total of 219 (157 genes in the two main clusters) genes showed differential expression between genetic types. Time series cluster analysis indicated that Casertana breed had a different pattern of gene expressions compared to the Large White and the crossbreed. For Casertana pigs, a first cluster showed 105 genes under expressed at 3 months of age and a second cluster indicated 52 genes over expressed at 3 months of age, in comparison to the other genetic types. As expected, some of the differentially expressed genes were in the category of “contractile fiber” and transcriptional factors involved in muscle development and differentiation.
Lycopene Inhibits NF-kB-Mediated IL-8 Expression and Changes Redox and PPARγ Signalling in Cigarette Smoke–Stimulated Macrophages
Rossella E. Simone,Marco Russo,Assunta Catalano,Giovanni Monego,Kati Froehlich,Volker Boehm,Paola Palozza
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019652
Abstract: Increasing evidence suggests that lycopene, the major carotenoid present in tomato, may be preventive against smoke-induced cell damage. However, the mechanisms of such a prevention are still unclear. The aim of this study was to investigate the role of lycopene on the production of the pro-inflammatory cytokine IL-8 induced by cigarette smoke and the possible mechanisms implicated. Therefore, human THP-1 macrophages were exposed to cigarette smoke extract (CSE), alone and following a 6-h pre-treatment with lycopene (0.5–2 μM). CSE enhanced IL-8 production in a time- and a dose-dependent manner. Lycopene pre-treatment resulted in a significant inhibition of CSE-induced IL-8 expression at both mRNA and protein levels. NF-kB controlled the transcription of IL-8 induced by CSE, since PDTC prevented such a production. Lycopene suppressed CSE-induced NF-kB DNA binding, NF-kB/p65 nuclear translocation and phosphorylation of IKKα and IkBα. Such an inhibition was accompanied by a decrease in CSE-induced ROS production and NOX-4 expression. Lycopene further inhibited CSE-induced phosphorylation of the redox-sensitive ERK1/2, JNK and p38 MAPKs. Moreover, the carotenoid increased PPARγ levels which, in turn, enhanced PTEN expression and decreased pAKT levels in CSE-exposed cells. Such effects were abolished by the PPARγ inhibitor GW9662. Taken together, our data indicate that lycopene prevented CSE-induced IL-8 production through a mechanism involving an inactivation of NF-kB. NF-kB inactivation was accompanied by an inhibition of redox signalling and an activation of PPARγ signalling. The ability of lycopene in inhibiting IL-8 production, NF-kB/p65 nuclear translocation, and redox signalling and in increasing PPARγ expression was also found in isolated rat alveolar macrophages exposed to CSE. These findings provide novel data on new molecular mechanisms by which lycopene regulates cigarette smoke-driven inflammation in human macrophages.
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