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Search Results: 1 - 10 of 33990 matches for " Fernández Llanes "
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Reflexiones sobre la ética en la investigación clínica y epidemiológica Reflections on clinical and epidemiological research ethics
Leticia M Fernández Garrote,Leticia Llanes Fernández,Eduardo Llanes Llanes
Revista Cubana de Salud Pública , 2004,
Abstract: La mayoría de las tecnologías en salud actúan directamente sobre el cuerpo y la mente humanos por lo tanto siempre existen riesgos biológicos o psicológicos por los que tomar en cuenta la seguridad de los individuos. También debemos pensar en el riesgo que se somete a los individuos cuando no se tiene al personal suficientemente adiestrado con pocas motivaciones o con una infraestructura sanitaria inadecuada. A pesar de la nobleza y beneficio potencial para las poblaciones que entra a el desarrollo de investigaciones tanto en pacientes como en sujetos sanos no debemos despreciar los elementos que conducen a riesgos para los sujetos participantes y un beneficio individual a veces muy peque o. Por estas razones es responsabilidad tanto de los investigadores participantes como de las autoridades que aprueban y financian estos estudios garantizar que el riesgo sea minimizado y los beneficios potenciales maximizados. Los cambios en la relación médico-paciente, la mayor especialización y las nuevas posibilidades de tecnologías médicas nos llevan a reflexionar sobre las consecuencias y los efectos a largo alcance desde el punto de vista ético, económico, demográfico, psicológico y jurídico Most health technologies directly act upon the human body and mind, therefore, there are always biological or psychological risks to be taken into consideration in relation to the safety of individuals. We must also think about the risks that an individual may take when the available staff is poorly trained or motivated or when the sanitary infrastructure is not appropriate. In spite of the potential benefits that the performance of research works on both patients and healthy subjects may bring to the populations, we should not ignore the elements that lead to risks for participating subjects and sometimes very small individual benefits. For these reasons, it is the responsibility of both the participating researchers and the authorities approving and financing the studies to assure minimum risks and great potential benefits. Changes in the physician-patient relationships, higher specialization and new possibilities of medical technologies lead us to make reflections on long-range consequences and effects from ethical, economic, demographic, psychological and legal viewpoints
DERIVADOS DEL 2-FURILETILENO INDUCEN LA MUERTE CELULAR PROGRAMADA EN CéLULAS CHO DE MAMíFERO
Hernández OC,Pimentel H,Llanes LL,Fernández S
Electronic Journal of Biomedicine , 2012,
Abstract: Aim: to evaluate the morphological and physiological changes induced by the synthesized products G1 and UC245 and the apoptotic process using CHO cell culture. Material and Methods: the Ca2+ uptake, DNA fragmentation in 1,8% agarose gel electrophoresis, nuclear morphology and mitotic index were studied as evidence of programated death cell. Results: the cytosolic Ca2+ increase and DNA fragmentation observed in association with nuclear morphology changes were consistent as molecular mark of apoptosis induced by the 2-furylethylene derivatives studied. Conclusions: these two synthesized products could confirm an inductor effect on apoptotic mechanism of this cell line.
Consideraciones para la implementación de un sistema nacional de toma y envío de muestras biológicas
Fernández Llanes,Roberto Juvenal; Weng Alemán,Zulia;
Revista Cubana de Higiene y Epidemiolog?-a , 2008,
Abstract: aim: to assess the considerations for the implementation of a national system for taking and sending biological samples. development: we made a general assessment on the conditions existing in the country in relation to the transportation of biological samples, common practice among laboratories with different purposes, on the antecedents connected with the topic in the world and in cuba, as well as on the present state of the sending of samples, and its characteristics in the national health system. conclusions: the authors highlight the advantage of having an organized and functional national health system within the structure of the socialist state to achieve in a near future the implementation of a national system for taking and sending biological samples.
Consideraciones para la implementación de un sistema nacional de toma y envío de muestras biológicas Considerations for the implementation of a national system for taking and sending biological samples
Roberto Juvenal Fernández Llanes,Zulia Weng Alemán
Revista Cubana de Higiene y Epidemiología , 2008,
Abstract: OBJETIVO: valorar consideraciones para la implementación de un sistema nacional de toma y envío de muestras biológicas. DESARROLLO: se hizo una valoración general sobre las condiciones existentes en el país relacionadas con la transportación de muestras biológicas, práctica común entre laboratorios con propósitos diversos, sobre los antecedentes relacionados con el tema en el mundo y en Cuba, así como sobre el estado actual del envío de muestras y sus características en el sistema nacional de salud. CONCLUSIONES: se destaca la ventaja que representa contar con un sistema nacional de salud organizado y funcional dentro de la estructura del Estado socialista, para lograr en un futuro no muy lejano la implementación de un sistema nacional de toma y envío de muestras biológicas. AIM: To assess the considerations for the implementation of a national system for taking and sending biological samples. DEVELOPMENT: We made a general assessment on the conditions existing in the country in relation to the transportation of biological samples, common practice among laboratories with different purposes, on the antecedents connected with the topic in the world and in Cuba, as well as on the present state of the sending of samples, and its characteristics in the national health system. CONCLUSIONS: The authors highlight the advantage of having an organized and functional national health system within the structure of the socialist State to achieve in a near future the implementation of a national system for taking and sending biological samples.
Desarrollo de un método para determinar la concentración mínima inhibitoria en cepas de referencias de leptospiras
Obregón,Ana Margarita; Llanes,Rafael; Fernández,Carmen; Hernández,Idialis; Rodríguez,José;
Revista Cubana de Medicina Tropical , 2005,
Abstract: a method to determine the minimum inhibitory concentration for leptospiras was developed, since there is not a standard method to measure it at the international level. reference strains from the pathogenic complex l. interrogans and l. biflexa were used against penicillin, cyprofloxacine, chloramphenicol, rifampicine and tetracycline. the minimum inhibitory concentration was defined as the lowest concentration of antibiotic where it was observed the inhibition of the bacterial mobility by direct examination in dark field. ranges for penicllin were from 0.095 to 152 μg/ml, for tetracycline from 0.156 to 3.13 μg/ml, for chloramphenicol, from 0.08 to 12.52 μg/ml, for rifampicine from 0.08 to 1.56 μg/ml, and for cyprofloxacine from 0.15 to 2.4 μg/ml. the antibiotics that showed the lowest values were cyprofloxacine, rifampicine and tetracycline, whereas the most elevated value was obtained against chloramphenicol and penicllin. the strains from the serogroups circulating more frequently in cuba were used in this research. this study will allow in a near future to determine the antimicrobial susceptibility in autochthonous strains isolated from patients with leptospirosis at the national level.
Desarrollo de un método para determinar la concentración mínima inhibitoria en cepas de referencias de leptospiras Development of a method to determine the minimum inhibitory concentration in reference strains from leptospiras
Ana Margarita Obregón,Rafael Llanes,Carmen Fernández,Idialis Hernández
Revista Cubana de Medicina Tropical , 2005,
Abstract: Se desarrolló un método para determinar la concentración mínima inhibitoria para las leptospiras, porque internacionalmente no existe un método estándar para medirla. Se utilizaron cepas de referencia, pertenecientes al complejo patogénico L. interrogans y L. biflexa frente a la penicilina, la ciprofloxacina, el cloranfenicol, la rifampicina, y la tetraciclina. La concentración mínima inhibitoria, fue definida como la menor concentración del antibiótico donde se observó la inhibición de la movilidad bacteriana por examen directo en campo oscuro. Los rangos fluctuaron para la penicilina desde 0,095 hasta 12-52 μg/mL, para la tetraciclina desde 0,156 hasta 3,13 μg/mL, para el cloranfenicol desde 0,08 hasta 12,52 μg/mL, para la rifampicina desde 0,08 μg/mL y hasta 1,56 μg/mL y para la ciprofloxacina desde 0,15 hasta 2,4 μg/mL. Los antibióticos que presentaron los valores más bajos fueron la ciprofloxacina, la rifampicina y la tetraciclina y el valor más elevado se obtuvo frente el cloranfenicol y la penicilina. En esta investigación fueron empleadas las cepas de los serogrupos que circulan más frecuentemente en Cuba. Este estudio permitirá en un futuro cercano, determinar la susceptibilidad antimicrobiana en cepas autóctonas aisladas de pacientes con leptospirosis, al nivel nacional. A method to determine the minimum inhibitory concentration for leptospiras was developed, since there is not a standard method to measure it at the international level. Reference strains from the pathogenic complex L. interrogans and L. biflexa were used against penicillin, cyprofloxacine, chloramphenicol, rifampicine and tetracycline. The minimum inhibitory concentration was defined as the lowest concentration of antibiotic where it was observed the inhibition of the bacterial mobility by direct examination in dark field. Ranges for penicllin were from 0.095 to 152 μg/mL, for tetracycline from 0.156 to 3.13 μg/mL, for chloramphenicol, from 0.08 to 12.52 μg/mL, for rifampicine from 0.08 to 1.56 μg/mL, and for cyprofloxacine from 0.15 to 2.4 μg/mL. The antibiotics that showed the lowest values were cyprofloxacine, rifampicine and tetracycline, whereas the most elevated value was obtained against chloramphenicol and penicllin. The strains from the serogroups circulating more frequently in Cuba were used in this research. This study will allow in a near future to determine the antimicrobial susceptibility in autochthonous strains isolated from patients with Leptospirosis at the national level.
Anomalías dentomaxilofaciales en dientes permanentes y su relación con traumas en la primera infancia
Corrales León,Arlene Liset; Serrano Fernández,Danilo; LLanes Serantes,Maribel;
Revista de Ciencias M??dicas de Pinar del R?-o , 2009,
Abstract: a descriptive, longitudinal and retrospective research was carried out including children from 7 to 14 years old belonging to the health area of "guama" dental university clinic in pinar del rio municipality, from february 2003 to march 2005 aimed at determining the presence of dental-maxillary-facial anomalies in permanent teeth and the relation to traumas in early childhood. the universe of the research was comprised of 250 children and of them a sample of 60 children was chosen, giving them a form to collect data for the research. results obtained were statistically processed through the statistic automatic system and the chi-squared test in its application of kindness-adjustment and spss package for the proportion and comparison tests. in all cases 0, 05 was the level of significance pre-fixed. among the most significant global results obtained, those traumas occurring between 5-6 years of age caused the greater number of dental-maxillary-facial anomalies in permanent teeth. male sex presented the traumas that most affected permanent teeth directly. the most frequent were the hypoplasia of the enamel and other non-orthodontic entities; female sex had a greater psychosocial implication due to these anomalies. orientations to dentists, parents and teachers were given about the relation among traumas and dental-maxillary-facial anomalies in permanent teeth.
Sensibilidad in vitro a la nistatina de aislamientos vaginales de Candida spp
Fernández Andreu,Carlos Manuel; Echemendía Medina,Yamilé; Cartaya González,Tahamara; Mendoza Llanes,Dianeya;
Revista Cubana de Medicina Tropical , 2001,
Abstract: the minimum inhibitory concentration (mic) of nistatine, one of the most used antifungal agents for this micosis, was determined in 68 candida strains isolated from vaginal smears. candida albicans repre-sented 75% of the total strains whereas c. parapsilosis, c. krusei and c. glabrata were much less frequently found. the predisposing factors were pregnancy and antibacterial treatment whereas leukorrhea and itching were the prevailing symptoms in most of the cases. mic values from the use of a broth dilution method ranged from 0,5-8mg/ml and the geometric mean was 1.36 mg/ml. for c. albicans, mic was 4 mg/ml due to two strains that showed the highest mic values (8 mg/ml). similarly, the strains showed low mic values, this means that therapeutic failures are not inherent to the emergence of resistant strains.
Anestesia subtenoniana en cirugía de estrabismo
Naranjo Fernández,Rosa M.; Pons Castro,Lucy; Pedroso Llanes,Alina; Padilla González,Carmen M.;
Revista Cubana de Oftalmolog?-a , 2004,
Abstract: a descriptive and retrospective study of 30 patients with the diagnosis of strabismus that were operated on at the service of pediatric ophthalmology and strabismus of "ramón pando ferrer" ophthalmological hospital by the technique of sub-tenon's anesthesia from april 2003 to may 2004 was conducted.the following variables were analyzed: age, diagnostic entity and pain degree. it was found a predominance of the age range 20-39. esotropia was the most frequent diagnosis. the pain degree was significant in the muscular traction and the sub-tenon's anesthetic technique proved to be effective independently of the surgical technique applied, without complications and with good analgesia.
Primer reporte del empleo de marcadores ISTR en Cactaceae (Pilosocereus sp)
Montalvo Fernández Grecia,Ortiz García Matilde,Quiala Mendoza Elisa,Keb-Llanes Miguel
Revista Colombiana de Biotecnología , 2010,
Abstract: Título en inglés: First report of the employment of ISTR markers in Cactaceae (Pilosocereus sp) Resumen Pilosocereus sp es una especie en peligro crítico de extinción, la única población conocida se encuentra en una mina de mármol verde, hoy abandonada, en la que su explotación produjo la disminución del 80% de la población en 3 a os; en la actualidad quedan 28 ejemplares, de ellos unos pocos son adultos, de los cuales solo dos producen frutos. Una de las etapas necesaria para su recuperación es la producción de plántulas para realizar el reforzamiento de la población natural. Como las plantas obtenidas serán plantadas en condiciones naturales, donde se enfrentarán a diversas situaciones ambientales, es conveniente realizar un estudio de diversidad genética. El objetivo de este trabajo fue estimar la variabilidad genética de plántulas de Pilosocereus sp empleando la técnica Inverse Sequence Tagged Repeat (ISTR). Se realizó la germinación in vitro de semillas y se determinó la variabilidad genética de las plántulas obtenidas. Con el análisis molecular se detectaron un total de 97 bandas, de ellas el 62,8% fueron polimórficas. El mayor porcentaje de bandas polimórficas (85,7%) se obtuvo con la combinación de oligonucleótidos F6/B6. Con las combinaciones de oligonucleótidos empleados se detectaron de 4 a 6 patrones de banda diferentes. La heterocigosidad media esperada fue de 0,39. Palabras clave: Cactaceae; variabilidad; extinción; polimorfismo. Abstract Pilosocereus sp is a species in critical extinction danger, the only known population is in a mine of green marble, abandoned today, but it exploitation produced the decrease of the population's 80% in 3 years, at the present time they are 28 individuals, of them some few ones are mature, of those which alone two produce fruits. One of the necessary stages for their recovery is the seedlings production to carry out the natural population's reinforcement. As the obtained plants they will be planted under natural conditions, where they will face diverse environmental situations, it is convenient to carry out a study of genetic diversity. The objective of this work was to estimate the genetic variability using the technical Inverse Sequence Tagged Repeat (ISTR). In vitro germination of seeds of Pilosocereus sp and the genetic variability of the obtained seedlings was determined. With the molecular analysis a total of 97 bands were detected, of them 62.8% were polymorphic. The biggest percentage of polymorphism (85.7%) was obtained with the primer combination F6/B6. With the primer combinations employed were
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