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Search Results: 1 - 10 of 104163 matches for " Fengping Zhang "
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Gradient Estimates for Weak Solutions of -Harmonic Equations
Yao Fengping
Journal of Inequalities and Applications , 2010,
Abstract: We obtain gradient estimates in Orlicz spaces for weak solutions of -Harmonic Equations under the assumptions that satisfies some proper conditions and the given function satisfies some moderate growth condition. As a corollary we obtain -type regularity for such equations.
Local estimates for gradients of solutions to elliptic equations with variable exponents
Fengping Yao
Electronic Journal of Differential Equations , 2013,
Abstract: In this article we present local $L^infty$ estimates for the gradient of solutions to elliptic equations with variable exponents. Under proper conditions on the coefficients, we prove that $$ left| abla u ight|in L^{infty}_{loc} $$ for all weak solutions of $$ operatorname{div} (g(| abla u|^2,x) abla u )=0quad ext{in } Omega. $$
Gradient Estimates for Weak Solutions of -Harmonic Equations
Fengping Yao
Journal of Inequalities and Applications , 2010, DOI: 10.1155/2010/685046
Abstract: We obtain gradient estimates in Orlicz spaces for weak solutions of -Harmonic Equations under the assumptions that satisfies some proper conditions and the given function satisfies some moderate growth condition. As a corollary we obtain Lp-type regularity for such equations.
Global Optimal Regularity for the Parabolic Polyharmonic Equations
Yao Fengping
Boundary Value Problems , 2010,
Abstract: We show the global regularity estimates for the following parabolic polyharmonic equation in under proper conditions. Moreover, it will be verified that these conditions are necessary for the simplest heat equation in .
Global Optimal Regularity for the Parabolic Polyharmonic Equations
Fengping Yao
Boundary Value Problems , 2010, DOI: 10.1155/2010/879821
Abstract: We show the global regularity estimates for the following parabolic polyharmonic equation ut+( Δ)mu=f in n×(0,∞),m∈ + under proper conditions. Moreover, it will be verified that these conditions are necessary for the simplest heat equation ut Δu=f in n×(0,∞).
Expression of the intact C4 typepepc gene cloned from maize in transgenic winter wheat
Xuqing Chen,Xiaodong Zhang,Rongqi Liang,Liquan Zhang,Fengping Yang,Mingqing Cao
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03185779
Abstract: Maize intactC 4-pepc gene was amplified through LA-PCR and successfully sub-cloned into modified vector pGreen0029 to form a stable expression construct named as pBAC214 (12 kb), which contains CaMV 35S promoter drivenbar gene as selection marker. Comparing the cloned DNA sequences (6.7 kb) with published maizeC 4-pepc gene (GenBank accession E17154) sequences, the identity of DNA sequence alignment is 98.96%. There are only 49 differences between these two intact DNA sequences, of which 13 occur in the region of promoter, 18 in introns, and 18 in exons. The homology of mRNA sequence alignment is 99.38%, and the putative amino acids sequence identity is 99.38% There are only 15 differences between these two mRNA, and these differences bring 4 sites mutant on the putative amino acids of PEPC protein. Through biolistic bombardment of PDS1000/He system, expression vector pBAC214 has been transformed into winter wheat. Southern blotting results show that the intactC 4 pepc gene has been integrated into genome of winter wheat. SDS-PAGE analysis of leaf soluble protein in transgenic wheat showed that the intactC 4-pepc gene was well transcribed, spliced and translated as in maize. The enzyme activity of leaf PEPC in transgenic wheat has been detected. The activities of leaf PEPC increased over 3–5 times in some transgenic plants. The data of photosynthesis rate and transpiration rate of transgenic wheat flag leaves showed that theC 4-pepc gene can increase the photosynthesis rate and transpiration rate of transgenic wheat.
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat
Xiaodong Zhang,Rongqi Liang,Xüqing Chen,Fengping Yang,Liquan Zhang
Chinese Science Bulletin , 2003, DOI: 10.1007/BF03187050
Abstract: The expression vector pBPC30, which carries the high molecular weight glutenin subunit (HMW-GS)1Dx5 and1Dy10 genes, was transferred into hexaploid winter wheat cv. Jinghua No. 1, Jing411 and Jingdong No. 6 explants of immature embryos and immature inflorescence by particle bombardment. A. large number of resistant transgenic plants were obtained under the selection of herbicide bialaphos or phosphinothricin (PPT). Confirmed transgenic plants of T0 generation showed successful integration of HMW-GS genes and bar gene into the wheat genome. T1 generation of transgenic plants can resist 20–150 mg/L PPT. Protein analysis of T2 seed by SDS-PAGE showed that HMW-GS1Dx5 and1Dy10 genes were well expressed in offspring seed of transgenic lines by co-expression with or substitution of endogenous1Dx2 or1Dy10. In one transgenic line, TG3-74, a new protein band between endogenous protein subunits 7 and 8 (marked as 8*) of glutenin appeared, but endogenous subunit 8 (encoded by lBy8 gene) was absent. Analysis of gluten rheological quality on seed proteins of 102 T3 plants showed that the sedimentation value of 5 transgenic lines (44.2–49.0 mL) was remarkably improved, 59.6%–64.3% higher than that of wild type Jinghua No. 1 and Jingdong No. 6, similar to bread wheat Cheyenne (48.0 mL). Analysis of dough rheological properties of transgenic lines showed that the dough stable time of 5 transgenic lines range from 16 to 30 min, whereas the dough stable time of wild type was only between 3–7 min. Our research suggests that introducing novel HMW-GS genes into wheat is an efficient way to improve its bread-making quality.
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS)genes in winter wheat

ZHANG Xiaodong,LIANG Rongqi,YANG Fengping,Zhang Liquan,
CHEN
,Xüqing

科学通报(英文版) , 2003,
Abstract: The expression vector pBPC30, which carries the high molecular weight glutenin subunit (HMW-GS) 1Dx5 and 1Dy10 genes, was transferred into hexaploid winter wheat cv. Jinghua No. 1, Jing411 and Jingdong No. 6 ex-plants of immature embryos and immature inflorescence by particle bombardment. A large number of resistant trans-genic plants were obtained under the selection of herbicide bialaphos or phosphinothricin (PPT). Confirmed transgenic plants of T0 generation showed successful integration of HMW-GS genes and bar gene into the wheat genome. T1 generation of transgenic plants can resist 20150 mg/L PPT. Protein analysis of T2 seed by SDS-PAGE showed that HMW-GS 1Dx5 and 1Dy10 genes were well expressed in offspring seed of transgenic lines by co-expression with or substitution of endogenous 1Dx2 or 1Dy10. In one transgenic line, TG3-74, a new protein band between endogenous pro-tein subunits 7 and 8 (marked as 8*) of glutenin appeared, but endogenous subunit 8 (encoded by 1By8 gene) was ab-sent. Analysis of gluten rheological quality on seed proteins of 102 T3 plants showed that the sedimentation value of 5 transgenic lines (44.249.0 mL) was remarkably improved, 59.6%64.3% higher than that of wild type Jinghua No. 1 and Jingdong No. 6, similar to bread wheat Cheyenne (48.0 mL). Analysis of dough rheological properties of transgenic lines showed that the dough stable time of 5 transgenic lines range from 16 to 30 min, whereas the dough stable time of wild type was only between 37 min. Our research suggests that introducing novel HMW-GS genes into wheat is an effi-cient way to improve its bread-making quality.
Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor
Yu Zhang, Lo?s Maignien, Xianxian Zhao, Fengping Wang, Nico Boon
BMC Microbiology , 2011, DOI: 10.1186/1471-2180-11-137
Abstract: In this study we analysed the long-term enrichment (286 days) of this microbial community, which was mediating SR-AOM in a continuous high-pressure bioreactor. 99.7% of the total biovolume represented cells in the form of small aggregates (diameter less then 15 μm). An increase of the total biovolume was observed (2.5 times). After 286 days, the ANME-2 (anaerobic methanotrophic archaea subgroup 2) and SRB (sulphate reducing bacteria) increased with a factor 12.5 and 8.4, respectively.This paper reports a net biomass growth of communities involved in SR-AOM, incubated at high-pressure.Anaerobic oxidation of methane coupled to sulphate reduction (SR-AOM) is a major process determining deep-sea geochemistry and cold-seep ecosystems. First of all, it controls the atmospheric methane efflux from the ocean floor, consuming more than 90% of the methane produced in marine sediments [1]. Moreover, it fuels the deep sea ecosystem by channelling thermal generated and biogenetic methane into organic matter and carbonate. Finally, SR-AOM shapes the sea floor landscape by contributing to bicarbonate and alkalinity production, resulting in massive carbonate precipitation [2]. The overall SR-AOM reaction is:Two groups of microorganisms are the key players in SR-AOM process: anaerobic methanotrophic archaea (ANME) with three groups (ANME-1, ANME-2 and ANME-3) and sulphate reducing bacteria (SRB) [3-6]. All ANME groups discovered so far are related clades of methanogens, while their SRB partner was always found in the same environment with or without forming spatial closely related consortia [7]. However, neither ANME nor SRB from SR-AOM active spots has been obtained in pure culture yet. The main difficulty lies on the extremely long doubling time (several months) and low growth yield (0.05 g dry weight/g carbon oxidized) of ANME and SRB from in vitro incubations [8-10].To stimulate the in vitro SR-AOM activity and to enrich the SR-AOM community, different types of bioreactors, whic
The synthesis of astaxanthin esters, independent of the formation of cysts, highly correlates with the synthesis of fatty acids in Haematococcus pluvialis
FengPing Miao,DaYan Lu,ChengWu Zhang,JinCheng Zuo,YaHong Geng,HongJun Hu,YeGuang Li
Science China Life Sciences , 2008, DOI: 10.1007/s11427-008-0141-6
Abstract: The compositions and contents of astaxanthin esters and fatty acids in four types of Haematococcus pluvialis cells were studied by HPLC and GC-MS. Results showed that the synthesis and accumulation of astaxanthin was independent of the formation of cysts, but was highly correlated with the synthesis and accumulation of fatty acids, though it is an well known phenomenon that the accumulation of astaxanthin is usually accompanied by the formation of cyst. The red cysts contain more than 30% of fatty acids, with 81% of the unsaturated fatty acids. Taken together, besides a resource of astaxanthin, H. pluvialis would be a good resource of valuable fatty acids.
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