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Search Results: 1 - 10 of 62607 matches for " Fang-Rong Yan "
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Sparse Logistic Regression for Diagnosis of Liver Fibrosis in Rat by Using SCAD-Penalized Likelihood
Fang-Rong Yan,Jin-Guan Lin,Yu Liu
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/875309
Abstract: The objective of the present study is to find out the quantitative relationship between progression of liver fibrosis and the levels of certain serum markers using mathematic model. We provide the sparse logistic regression by using smoothly clipped absolute deviation (SCAD) penalized function to diagnose the liver fibrosis in rats. Not only does it give a sparse solution with high accuracy, it also provides the users with the precise probabilities of classification with the class information. In the simulative case and the experiment case, the proposed method is comparable to the stepwise linear discriminant analysis (SLDA) and the sparse logistic regression with least absolute shrinkage and selection operator (LASSO) penalty, by using receiver operating characteristic (ROC) with bayesian bootstrap estimating area under the curve (AUC) diagnostic sensitivity for selected variable. Results show that the new approach provides a good correlation between the serum marker levels and the liver fibrosis induced by thioacetamide (TAA) in rats. Meanwhile, this approach might also be used in predicting the development of liver cirrhosis.
Bayesian Inference for Generalized Linear Mixed Model Based on the Multivariate t Distribution in Population Pharmacokinetic Study
Fang-Rong Yan, Yuan Huang, Jun-Lin Liu, Tao Lu, Jin-Guan Lin
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058369
Abstract: This article provides a fully Bayesian approach for modeling of single-dose and complete pharmacokinetic data in a population pharmacokinetic (PK) model. To overcome the impact of outliers and the difficulty of computation, a generalized linear model is chosen with the hypothesis that the errors follow a multivariate Student t distribution which is a heavy-tailed distribution. The aim of this study is to investigate and implement the performance of the multivariate t distribution to analyze population pharmacokinetic data. Bayesian predictive inferences and the Metropolis-Hastings algorithm schemes are used to process the intractable posterior integration. The precision and accuracy of the proposed model are illustrated by the simulating data and a real example of theophylline data.
Empirical Likelihood Estimation for Population Pharmacokinetic Study Based on Generalized Linear Model
Fang-rong Yan,Jin-guan Lin,Yuan Huang,Jun-lin Liu,Tao Lu
Journal of Applied Mathematics , 2012, DOI: 10.1155/2012/250909
Abstract: To obtain efficient estimation of parameters is a major objective in population pharmacokinetic study. In this paper, we propose an empirical likelihood-based method to analyze the population pharmacokinetic data based on the generalized linear model. A nonparametric version of the Wilk's theorem for the limiting distributions of the empirical likelihood ratio is derived. Simulations are conducted to demonstrate the accuracy and efficiency of empirical likelihood method. An application illustrating our methods and supporting the simulation study results is presented. The results suggest that the proposed method is feasible for population pharmacokinetic data. 1. Introduction The parameter estimation in population pharmacokinetics (PPK) is a significant problem in clinical research. It is a novel problem in pharmacokinetic (PK) study that combines classic PK models with group statistical models. The PPK parameters, including group typical values, fixed effect parameter, interindividual variation, and intraindividual variation, which are the determinant factors of drug concentration in patients, are taken into consideration. PPK is capable of quantitatively describing the effects of different factors in drug metabolism, such as pathology, physiology, and combined medication, then providing guidance on the adjustment of therapeutic regimen, thus increasing the efficacy and safety in a new drug evaluation. Many statistical models have been proposed to fit PPK parameters. The most popular analytic statistical model for PPK data is the random effects model proposed by Larid and Ware [1]. Besides, the classic compartmental model and nonlinear mixed effect model proposed by Sheiner et al. in 1977 [2] are the most commonly used statistical models for PPK. And recently, generalized linear model proposed by Salway and Wakefield in 2008 [3] has received wide attention. In fact nonlinear models are used to estimate parameters of a chosen compartmental model. This model can provide generally good results. However, a disadvantage of the nonlinear models is that it is generally more difficult to fit. From a computational point of view, one also faces the usual challenges associated with nonlinear regression such as choosing starting values, problem with convergence, nonlinear regression diagnostics, and so forth. Other nonlinear models for the analysis of PPK data see [4–6]. Salway and Wakefield [3] proposed a generalized linear model with gamma distribution to deal with PPK data. The GLM is one of the most widely used regression models for statistical analysis. The
Combined analysis of endometrial thickness and pattern in predicting outcome of in vitro fertilization and embryo transfer: a retrospective cohort study
Shi-Ling Chen, Fang-Rong Wu, Chen Luo, Xin Chen, Xiao-Yun Shi, Hai-Yan Zheng, Yun-Ping Ni
Reproductive Biology and Endocrinology , 2010, DOI: 10.1186/1477-7827-8-30
Abstract: Cycles of IVF/ICSI-ET conducted between January 2003 and December 2008 at a university-based reproductive center were reviewed retrospectively. Endometrial ultrasonographic characteristics were recorded on the day of hCG administration. In the combined analysis, endometrial thickness groups (group 1: equal or <7 mm; group 2: 7-14 mm; group 3: >14 mm) were subdivided into two endometrial patterns (pattern A: triple-line; pattern B: no-triple line). Clinical pregnancy rate (CPR) and early miscarriage rate in different groups were analyzed.A total of 2896 cycles were reviewed. Clinical pregnancy rate (CPR) was 24.4% in group1-A. There were no second trimester pregnancies in group 1-B. Miscarriage rate in group 2-A was significantly lower compared to group 2-B (P < 0.01), although CPR did not show any significant differences between the groups. A no-triple line endometrial pattern with moderate endometrial thickness (7-14 mm) had a detrimental effect on pregnancy outcome, but not the occurrence of pregnancy. In group 3, there was no difference in CPR and miscarriage rates between the two patterns; adequate endometrial thickness (>14 mm) seemed to mitigate the detrimental impact (high miscarriage rate) of pattern B.Combined analysis of endometrial thickness and pattern on the day of hCG administration was a better predictor of the outcome of IVF/ICSI-ET and may be more helpful for patient counseling than the separate analyses.It is generally accepted that endometrial receptivity is critical to successful pregnancy. Ultrasonographic examination has been routinely performed in assisted reproduction technology (ART) treatments because of the accurate evaluation and noninvasive detection. Indeed, both endometrial thickness and endometrial pattern have been regarded as prognostic parameters for successful pregnancy in in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET).Following the periodic stimulation of ovarian hormones, the changes in
Withanolides-Induced Breast Cancer Cell Death Is Correlated with Their Ability to Inhibit Heat Protein 90
Hui-Chun Wang, Yi-Ling Tsai, Yang-Chang Wu, Fang-Rong Chang, Mei-Hsin Liu, Wen-Ying Chen, Chin-Chung Wu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0037764
Abstract: Withanolides are a large group of steroidal lactones found in Solanaceae plants that exhibit potential anticancer activities. We have previously demonstrated that a withanolide, tubocapsenolide A, induced cycle arrest and apoptosis in human breast cancer cells, which was associated with the inhibition of heat shock protein 90 (Hsp90). To investigate whether other withanolides are also capable of inhibiting Hsp90 and to analyze the structure-activity relationships, nine withanolides with different structural properties were tested in human breast cancer cells MDA-MB-231 and MCF-7 in the present study. Our data show that the 2,3-unsaturated double bond-containing withanolides inhibited Hsp90 function, as evidenced by selective depletion of Hsp90 client proteins and induction of Hsp70. The inhibitory effect of the withanolides on Hsp90 chaperone activity was further confirmed using in vivo heat shock luciferase activity recovery assays. Importantly, Hsp90 inhibition by the withanolides was correlated with their ability to induce cancer cell death. In addition, the withanolides reduced constitutive NF-κB activation by depleting IκB kinase complex (IKK) through inhibition of Hsp90. In estrogen receptor (ER)-positive MCF-7 cells, the withanolides also reduced the expression of ER, and this may be partly due to Hsp90 inhibition. Taken together, our results suggest that Hsp90 inhibition is a general feature of cytotoxic withanolides and plays an important role in their anticancer activity.
New Benzoyl Glucosides and Cytotoxic Pterosin Sesquiterpenes from Pteris ensiformis Burm.
Yung-Husan Chen,Fang-Rong Chang,Mei-Chin Lu,Pei-Wen Hsieh,Ming-Jiuan Wu,Ying-Chi Du,Yang-Chang Wu
Molecules , 2008, DOI: 10.3390/molecules13020255
Abstract: Three new compounds: 2R,3R-pterosin L 3-O-β-D-glucopyranoside (1), β-Dxylopyranosyl(1→2)-7-O-benzoyl-β-D-glucopyranoside (2) and 4-O-benzoyl-β-D-xylopyranosyl(1→2)-7-O-benzoyl-β-D-glucopyranoside (3), together with nine knowncompounds, were isolated from the ethyl acetate extract of Pteris ensiformis. 5-[2-Hydroxyethylidene]-2(5H)-furanone (4), which had been synthesized, was isolated fromnatural sources for the first time. The structures of all isolated compounds were determinedon the basis of mass and spectroscopic evidence. Compound 1 and pterosin B (5) showcytotoxicity against HL 60 cells (human leukemia) with the IC50 values of 3.7 and 8.7μg/mL, respectively.
An Aluminum Microfluidic Chip Fabrication Using a Convenient Micromilling Process for Fluorescent Poly(DL-lactide-co-glycolide) Microparticle Generation
Yung-Sheng Lin,Chih-Hui Yang,Chih-Yu Wang,Fang-Rong Chang,Keng-Shiang Huang,Wan-Chen Hsieh
Sensors , 2012, DOI: 10.3390/s120201455
Abstract: This study presents the development of a robust aluminum-based microfluidic chip fabricated by conventional mechanical micromachining (computer numerical control-based micro-milling process). It applied the aluminum-based microfluidic chip to form poly(lactic-co-glycolic acid) (PLGA) microparticles encapsulating CdSe/ZnS quantum dots (QDs). A cross-flow design and flow-focusing system were employed to control the oil-in-water (o/w) emulsification to ensure the generation of uniformly-sized droplets. The size of the droplets could be tuned by adjusting the flow rates of the water and oil phases. The proposed microfluidic platform is easy to fabricate, set up, organize as well as program, and is valuable for further applications under harsh reaction conditions (high temperature and/or strong organic solvent systems). The proposed method has the advantages of actively controlling the droplet diameter, with a narrow size distribution, good sphericity, as well as being a simple process with a high throughput. In addition to the fluorescent PLGA microparticles in this study, this approach can also be applied to many applications in the pharmaceutical and biomedical area.
Efficient Synthesis of Boron-Containing α-Acyloxyamide Analogs via Microwave Irradiation
Chih-Cheng Chai,Pin-Yi Liu,Chia-Hung Lin,Hsien-Chi Chen,Yang-Chang Wu,Fang-Rong Chang,Po-Shen Pan
Molecules , 2013, DOI: 10.3390/molecules18089488
Abstract: In this report, a Passerini three-component reaction utilizing boron-containing carboxylic acids or aldehydes is discussed. The reaction was carried out in water and facilitated by the use of microwave irradiation. This methodology allowed for the efficient formation of a broad range of boron-containing α-acyloxyamides under mild conditions within a short time. Two series of boron-containing α-acyloxyamides were synthesized and subsequently screened for cytotoxicity using the MTT cell viability assay. Two potential lead compounds were found to have potent activity against the HepG2 cancer cell line, demonstrating the potential of this methodology for use in the development of novel pharmaceuticals.
Camptothecinoids from the seeds of Taiwanese Nothapodytes foetida
Shou-Fang Wu,Pei-Wen Hsieh,Chin-Chung Wu,Chia-Lin Lee,Shu-Li Chen,Chi-Yu Lu,Tian-Shung Wu,Fang-Rong Chang,Yang-Chang Wu
Molecules , 2008, DOI: 10.3390/molecules13061361
Abstract: Two new alkaloids, 9-methoxy-18,19-dehydrocamptothecin (1) and 5- hydroxymappicine-20-O-β-glucopyranoside (2a/2b as a racemic mixture), together with nine known compounds: camptothecin (3), 9-methoxy-camptothecin (4), 5-hydroxycamptothecin (5a/5b racemic mixture), 5-hydroxy-9-methoxycamptothecin (6a/6b racemic mixture), diosmetin (7), apigenin (8), apigenin-7-O-glucopyranoside (9), rosin (cinnamyl- O-β-D-glucopyranoside) (10) and amarantholidoside IV (11) were isolated from the immature seeds of Nothapodytes foetida (Wight) Sleumer. The structures were elucidated by spectroscopic analyses. In the present research, compounds 1, 3, 4, 5a/5b and 6a/6b, also showed in vitro cytotoxicity against six cancer cell lines (HepG2, Hep3B, MDA-MB- 231, MCF-7, A549, and Ca9-22). Among them, compound 1 exhibited significant cytotoxicity against these cancer cell lines, with IC50 of 0.24-6.57 μM. Furthermore, HPLC profiles were developed for qualitative and quantitative analysis of these active constituents in different parts of this plant, including mature and immature seeds, leaves, stems and roots. The results revealed that compounds 3 and 4 have the highest concentrations, which are found in the roots part of the plant.
Antiproliferation and Induction of Apoptosis in Ca9-22 Oral Cancer Cells by Ethanolic Extract of Gracilaria tenuistipitata
Chi-Chen Yeh,Chao-Neng Tseng,Jing-Iong Yang,Hurng-Wern Huang,Yi Fang,Jen-Yang Tang,Fang-Rong Chang,Hsueh-Wei Chang
Molecules , 2012, DOI: 10.3390/molecules170910916
Abstract: The water extract of Gracilaria tenuistipitata have been found to be protective against oxidative stress-induced cellular DNA damage, but the biological function of the ethanolic extracts of G. tenuistipitata (EEGT) is still unknown. In this study, the effect of EEGT on oral squamous cell cancer (OSCC) Ca9-22 cell line was examined in terms of the cell proliferation and oxidative stress responses. The cell viability of EEGT-treated OSCC cells was significantly reduced in a dose-response manner (p < 0.0001). The annexin V intensity and pan-caspase activity of EEGT-treated OSCC cells were significantly increased in a dose-response manner (p < 0.05 to 0.0001). EEGT significantly increased the reactive oxygen species (ROS) level (p < 0.0001) and decreased the glutathione (GSH) level (p < 0.01) in a dose-response manner. The mitochondrial membrane potential (MMP) of EEGT-treated OSCC cells was significantly decreased in a dose-response manner (p < 0.005). In conclusion, we have demonstrated that EEGT induced the growth inhibition and apoptosis of OSCC cells, which was accompanied by ROS increase, GSH depletion, caspase activation, and mitochondrial depolarization. Therefore, EEGT may have potent antitumor effect against oral cancer cells.
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