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Search Results: 1 - 10 of 25011 matches for " Fagundes Letícia Martins "
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Criopreserva o de ovócitos de bovinos imaturos desnudados ou n o, utilizando o etilenoglicol pelo método da vitrifica o
Costa Eduardo Paulino da,Guimar?es José Domingos,Torres Ciro Alexandre Alves,Fagundes Letícia Martins
Revista Brasileira de Zootecnia , 2002,
Abstract: Objetivou-se avaliar os efeitos da vitrifica o em ovócitos de bovinos após o cultivo in vitro, utilizando o etilenoglicol como crioprotetor. Ovócitos obtidos de ovários de vacas abatidas em matadouro foram distribuídos aleatoriamente em três tratamentos. Tratamento 0 (testemunha): ovócitos n o-desnudados e n o-congelados. Tratamento 1: vitrifica o de ovócitos imaturos n o desnudados, desidratados previamente por cinco minutos em três solu es contendo 20, 20 e 40% de etilenoglicol, acrescidas de 0,3 mol L-1 de trehalose e 20% de PVP, em meio de Talp Hepes. Tratamento 2: vitrifica o de ovócitos imaturos desnudados, conforme o Tratamento 1. Após o descongelamento (imers o em banho-maria a 30oC por 20 segundos), os ovócitos foram reidratados gradativamente, mantendo-os por 6 minutos em cada uma das solu es a seguir, sucessivamente: meio Talp Hepes com 20% de etilenoglicol + 0,3 mol L-1 de trehalose + 10% de PVP e meio Talp Hepes sem etilenoglicol, trehalose e PVP, onde foram lavados três vezes. Posteriormente, os ovócitos foram cultivados a 38,5oC, com 95% de umidade e atmosfera de 5% de CO2 por 24 horas. Após o cultivo, os ovócitos foram fecundados e os embri es cultivados in vitro por sete dias. Foi encontrada uma taxa de matura o nuclear de 81 (68/84), 19 (7/36) e 0% (0/31), nos Tratamentos 0, 1 e 2, respectivamente. As taxas de clivagem e de desenvolvimento embrionário foram de 56,4 (102/181) e 54,9% (56/102), 1,7 (1/60) e 0,0% (1/60), 0,0 (0/71) e 0,0% (0/71), nos Tratamentos 0, 1 e 2, respectivamente. Esses resultados indicam que o procedimento de vitrifica o, segundo os protocolos utilizados, n o é indicado para a criopreserva o de ovócitos de bovinos.
Criopreserva??o de ovócitos de bovinos imaturos desnudados ou n?o, utilizando o etilenoglicol pelo método da vitrifica??o
Costa, Eduardo Paulino da;Guimar?es, José Domingos;Torres, Ciro Alexandre Alves;Fagundes, Letícia Martins;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000500008
Abstract: the objective was to evaluate the effects of vitrification of immature bovine oocytes after in vitro culture, by the use of cryoprotectors ethylene glycol. oocytes from cows ovaries from slaughters houses were randomly alocated into three treatments. treatment 0 (control): frozen-thawed undesnude oocytes; treatment 1, immature vitrificated undesnude oocytes dehydrated for 5 minutes in each of the following solutions of 20, 20 and 40% of ethylene glycol, respectively, associated to 0.3 mol l-1 of trehalose and 20% of pvp, in media talp hepes, and, treatment 2, the same as treatment 1, but desnudes oocytes. after frozen-thawed of the oocytes (imersion in water bath at 30oc for 20 seconds), the oocytes were gradually rehydrated, in the following sequence of solutions: media talp hepes with 20% of ethylene glycol + 0.3 mol l-1 of trehalose + 10% of pvp and media talp hepes without ethylene glycol, trehalose and pvp, were washed three times. ultimately, the oocytes were cultured at 38.5oc, with 95% umidity and atmosphere of 5% of co2 for 24 hours. after culture, the oocytes were fertilized and the embryos cultured in vitro for seven days. the nuclear maturation were 81 (68/84), 19 (7/36) and 0% (0/31), for treatments 0, 1 and 2, respectively. the cleavage and development rates were: 56.4(102/181) and 54,9% (56/102), 1,7. (1/60) and 0,0% (1/60), 0,0 (0/71) and 0,0% (0/71), for the treatments 1, 2 e 3, respectively. these results show that the vitrification procedures, by the used protocols, are not indicated for bovine oocytes cryopreservation.
Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
Congela??o de ovócitos desnudados ou n?o, maturos e imaturos de bovinos, utilizando o Etileno Glicol pelo método convencional
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Guimar?es, José Domingos;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000800014
Abstract: the objective of this study was to evaluate the cryopreservation of in vitro mature or immature oocytes by conventional method. the experiment was conducted using oocytes of cows' ovaries from slaughterhouse, distributed into six treatments: unfrozen oocytes with the cumulus oophorus cells (t1) and naked (t2) which were submitted to the process of miv, fiv and civ; immature oocytes, with the cumulus oophorus cells (t3) and naked (t4), which were submitted to the cryopreservation, unfrozen and the miv, fiv and civ; oocytes with the cumulus oophorus cells (t5) and naked (t6), which were submitted to in vitro maturation, cryopreservation, and fiv and civ. the oocytes were frozen by the conventional methods, dehydrated by the immersion in three solutions with 0.6; 1.2 and 1.8 mol l-1 of ethylene glycol (eg) during 5 minutes each phase. the thawing phase was done by the immersion in water-bath at 30 oc during 20 seconds, and so, the oocytes were re-hydrated in three phases (0.9 mol l-1 eg + 0.3 mol l-1 of sacarose; 0.3 l-1 of sacarose without eg and sacarose) for six minutes each one. the mainly ultrastructural changes in cryopreserved matured oocytes were prematurely released of cortical granules. however, the frozen immature and mature oocytes showed vacuolization and disappearance of cristae mitochondrial. the frozen immature oocytes showed the maturation rate of 82.5, 75.4, 9.2 and 5.8% for t1, t2, t3 and t4, respectively. the fecundation rate were 56.2, 0.0, 38.7, 8.6, 63.6 and 16.7% and from the cleavage were 36.3, 7.9, 0.4, 0.0, 0.0 and 0.0% for t1, t2, t3, t4, t5 and t6, respectively. morula and blastocyst were observed only for unfrozen and naked oocytes (t1) (34.5%). these results showed that the frozen protocols affect the viability of the oocytes.
Saúde mental do cuidador na institui??o hospitalar
Bertoletti, Juliana;Cabral, Patrícia Martins Fagundes;
Psicologia: Teoria e Pesquisa , 2007, DOI: 10.1590/S0102-37722007000100012
Abstract: this article has the purpose of verifying the laboural experiences of the nursing professionals and its consequences on their mental health. the research was made with nursing professionals of the instituto de cardiologia in porto alegre, who were dismissed from their activities during 2003 due to mental and behavioral disturbances (brief depressive disorder, recurrent depressive disorder and general anxiety), diagnosed according to cid-10. the data were collected through semi-structured individual interviews, and for their interpretation the technique of content analisys was used. the results show that aspects of work contributed to the process of falling ill of these professionals, such as the nature of the caretaker activities, overburden of tasks and dificulties of interpersonal relations. it was further verified the perception of the individuals as to the role of the organization vis-à-vis their dismissal, in order to increase the discussion about management of people and health promotion at work.
Qualidade pós-colheita de banana Prata An? armazenada sob diferentes condi??es
Viviani, Letícia;Leal, Paulo Martins;
Revista Brasileira de Fruticultura , 2007, DOI: 10.1590/S0100-29452007000300012
Abstract: the inadequate handling during the post-harvest has been responsible by the lost of value of the product in the internal market, and lost of opportunity of exportation of this brazilian fruit. the other observed aspect is that the commerce of banana is highly affected by the bad visual aspects of the fruits and inadequate packing. inside of this context, this research has, as major objective, the evaluation of post-harvest quality of banana fruits, variety prata an?. experiments were realized to evaluate the quality variation, appearance, and colorin until the fruits were inappropriate for consumption, using physical-chemical and sensorial analysis, by comparing cold storage at 13oc and maintenance of the products at natural environment with no temperature control. the fruits of banana during the experiments were packed in three different packing ( wood box type "torito" with capacity for 18kg and cardboard wrapper, wood box type "? caixa", with capacity for 13kg and cardboard box with capacity for 18kg). physical or mechanical damages were, also, evaluated during the transport of the fruits from the packing house to the distribution center, and after acclimatization. keeping the fruits at refrigerated environment increased the life storage in five days. the packing used did not promote difference in the conservation of the fruits, neither in the indicatives attributes of maturation and also in the increase of physical damages.
Sinopse do gênero Phyllanthus L. (Phyllanthaceae) do Estado de S?o Paulo
Martins, Erika Ramos;Lima, Letícia Ribes de;
Hoehnea , 2011, DOI: 10.1590/S2236-89062011000100011
Abstract: the genus phyllanthus occurs worldwide, and in brazil the latest survey points the occurrence of 107 species. the survey also shows that approximately one quarter of them are mostly found in rocky fields, cerrados and caatingas. the purpose of this study is to summarize the genus phyllanthus in order to contribute to the completion of the monograph of the family phyllanthaceae as a whole. furthermore, this study was aimed to better circurscribe the species that occur in s?o paulo state as well as present information morphology, geographical distribution and taxonomy about the taxa studied. in the state of sao paulo 16 species of the genus were found.
Revis?es sistemáticas de antibioticoprofilaxia em cesarianas
Martins, Ana Cristina Marques;Krauss-Silva, Letícia;
Cadernos de Saúde Pública , 2006, DOI: 10.1590/S0102-311X2006001200002
Abstract: this study reviews the available evidence on the efficacy of antibiotic prophylaxis in cesarean sections. the study included a detailed analysis of the quality of design and performance and the clinical heterogeneity of selected clinical trials. meta-analysis of placebo-controlled trials estimated an efficacy of some 65% for the two study endpoints, endometritis and surgical wound infection, corresponding to a decrease in infection rates of some 11% and 5%, respectively. sensitivity analyses showed summary effects similar to those observed for all studies. results for the non-elective cesarean sections subgroup (12 studies) indicated a relevant benefit for both endpoints, corresponding to a decrease in incidence rates of some 14% (endometritis) and 5% (surgical infection wound). for elective surgeries (two trials), no relevant benefit was found. evidence from comparative trials on number of doses was limited due to important methodological shortcomings and to the small number of patients enrolled (three trials). comparative drug trials (seven studies) did not show evidence of different performance between first and second-generation cephalosporins.
Qual o seu diagnóstico?
Machado Márcio Martins,Rosa Ana Cláudia Ferreira,Azeredo Letícia Martins,Barros Nestor de
Radiologia Brasileira , 2003,
Aspectos ultra-sonográficos dos hamartomas dos ductos biliares (complexo de von Meyenburg): resultado de uma busca ativa de oito anos
Machado Márcio Martins,Rosa Ana Cláudia Ferreira,Barros Nestor de,Azeredo Letícia Martins
Radiologia Brasileira , 2003,
Abstract: A correta identifica o de tumores hepáticos benignos é importante, pois a maioria n o necessita de conduta intervencionista. Os autores relatam os aspectos ultra-sonográficos dos hamartomas de ductos biliares (complexo de von Meyenburg) em 16 pacientes estudados prospectivamente, resultados de uma busca ativa de oito anos. Em 14 pacientes foram identificadas múltiplas les es menores ou iguais a 0,8 cm, e em dois, apenas duas les es em cada (medindo de 0,4 cm a 1,3 cm). O aspecto ultra-sonográfico que predominou foi o de múltiplas pequenas imagens hiperecogênicas com ou sem reverbera o sonora posterior e margens irregulares (14 pacientes). Menos comumente, foi encontrado o aspecto "em alvo", com centro com maior ecogenicidade que a periferia (dois pacientes com duas les es cada) e margens bem definidas.
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