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Search Results: 1 - 10 of 48848 matches for " FU Song-bin "
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Zymographic Analysis of MMP in Human Lung Adenocarcinoma Cell Lines with Differential Metastatic Potential
不同转移能力的肺腺癌细胞系金属蛋白酶活性分析 Zymographic Analysis of MMP in Human Lung Adenocarcinoma Cell Lines with Differential Metastatic Potential

YAN Cheng-hui,WU Yan,JIN Yan,FU Song-bin,
闫承慧
,吴焱,金焰,傅松滨YAN Cheng-hui,WU Yan,JIN Yan,FU Song-bin

遗传 , 2000,
Abstract: To investigate the relationship between the MMP activities and metastatic ability in human lung adenocarcinoma cell lines with differential metastatic potential.Nine lung adenocarcinoma cell lines were tested for their MMP activities by zymographic analysis methods.The MMP production capabilities of carcinoma cells rose following the increase of their metastatic potentials.The result suggested that there was some relationship between MMP-9 activties and metastatic potential in human lung adenocarcinomas.
Study on the Relationship Between the Resistance to MTX and the Transport Protein Superfamily of ATP-binding Cassette that Induces Multiple Drug Resistance
介导多药耐药的ABC转运蛋白超家族与MTX耐药性的关系研究

ZHANG Chun-Yu,FENG Yuan-Xi,LI Pu,FU Song-Bin,
张春玉
,冯源熙,李璞,傅松滨

遗传 , 2006,
Abstract: A major problem, especially the multidrug resistance, in chemotherapy was the resistance to the chemotherapeutic agents. ATP-binding cassette transporter superfamily that mediated the efflux of drugs was involved in multidrug resistance. In order to understand the relationship between the resistance to MTX and the transport protein superfamily of ATP-binding cassette, and to investigate the mechanism of resistance to MTX, the study detected the expressions of mdr1, mrp1, mrp2, mrp3, mrp5, mrp6 and abcg2 that encoded the transport proteins by SuperArray analysis and the expressions of MRPland MRP5 proteins by Western blot analysis. The results showed that the multidrug resistance proteins were the chief member of ATP-binding cassette transporter superfamily related to resistance to MTX. And the high expression levels of mrp1 and mrp5 were detected. Moreover, it revealed by SuperArray analysis that expression of mrp5 in MTX-resistant cells was significantly higher than that in normal mouse cells. Besides, corresponding excessive expression of MRP5 protein in MTX-resistant cells was also confirmed by Western blot. So, MRP5 could play important roles in the resistance to MTX and would be a new potential drug target.
The Roles of rab5a Gene in the Mechanism of Tumor Metastasis
rab5a基因在肿瘤转移中的作用研究

SHI Zhong-Cheng,YU Yang,LI Yu,FU Song-Bin,
史忠诚
,于旸,李钰,傅松滨

遗传 , 2005,
Abstract: To study the role of rab5a gene in the mechanism of tumor metastasis, rab5a gene was stable transfected into AGZY83-a cell, a lung adenocarcinoma cell line with low metastatic potential. Superarray was applied to study the effect of rab5a on the differential expression of genes related to tumor metastasis. Five differentially expressed genes were obtained, including one up-regulated gene s100a4 and 4 down-regulated genes, nm23a, rac1, cst3 and col4a2. These results were confirmed at both the RNA and protein levels. We concluded that rab5a promoted tumor metastasis by affecting multiple genes in several pathways involved in tumor metastasis.
Gastric cancer cell lines induced by trichostatin A
Xiao-Ming Zou, Yun-Long Li, Hao Wang, Wu Cui, Xiao-Lin Li, Song-Bin Fu, Hong-Chi Jiang
World Journal of Gastroenterology , 2008,
Abstract: AIM: To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901.METHODS: The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot.RESULTS: TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.CONCLUSION: TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.
Association Study of Apolipoprotein E Gene Polymorphism and Cerebral Infarction in Type 2 Diabetic Patients
载脂蛋白E基因多态性与2型 糖尿病脑梗死的关系Association Study of Apolipoprotein E Gene Polymorphism and Cerebral Infarction in Type 2 Diabetic Patients

ZHOU Jun,XUE Ya-li,GUAN Ya-Xin,YANG Yin-dong,FU Song-bin,ZHANG Jin-Chao,
周 君
,薛雅丽,关亚新,杨印东,傅松滨,张巾超ZHOU Jun,XUE Ya-Li,GUAN Ya-Xin,YANG Yin-Dong,FU Song-Bin,ZHANG Jin-Chao

遗传 , 2005,
Abstract: In order to explore the association of apolipoprotein E (ApoE) gene polymorphism with cerebral infarction in type 2 diabetic patients of Han nationality in Northeast China , the genotypes of ApoE gene were analyzed by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) in the 208 cases, including 69 cases in control (CON) group and 67 in type 2 diabetes mellitus (T2DM) group as well as 72 in type 2 diabetes mellitus with cerebral infarction (T2DMCI) group. Plasma lipid content in T2DMCI was also detected for 70 cases. The distribution of genotypes in ApoE gene,epsilon(2)epsilon(3),epsilon(3)epsilon(3) as well as epsilon(3)epsilon(4) was no significant difference in three groups (epsilon(2)epsilon(3) : 13.2%,epsilon(3)epsilon(3) : 67.6%,epsilon(3)epsilon(4) : 16.2%in CON group;epsilon(2)epsilon(3) : 19.4%,epsilon(3)epsilon(3): : 70.1%epsilon(3)epsilon(4) : 9%in T2DM group;epsilon(2)epsilon(3) : 15.2%,epsilon(3)epsilon(3) : 75%,epsilon(3)epsilon(4) : 4.2%in T2DMCI group).The allele frequencies of epsilon(2),epsilon(3) and epsilon(4) were not significantly different in the three groups, either(epsilon(2) : 9.6%,epsilon(3) : 82.4%,epsilon(4) : 8.1%in CON group; epsilon(2) :10.5%,epsilon(3) :84.3%,epsilon(4) : 5.2%in T2DM group; epsilon(2) :11.8%,epsilon(3) :84.7%,epsilon(4) : 3.5%in T2DMCI group). The levels of total cholesterol (TC), tryglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were not significantly different among the different genotypes in T2DMCI group. The study confirmed that the polymorphisms of ApoE gene are neither associated with the T2DMCI, nor with the levels of plasma lipid in T2DMCI.
Overexpression of Tumor Suppressor Gene P21 in a Pair of Lung Adenocarcinoma Cell Lines,Anip973 and AGZY83-a,with Different Metastasis Potential
高低转移肺腺癌细胞系Anip973和AGZY83-a中P21过表达的研究 Overexpression of Tumor Suppressor Gene P21 in a Pair of Lung Adenocarcinoma Cell Lines,Anip973 and AGZY83-a,with Different Metastasis Potential

WANG Bai-qiu,YAN Cheng-hui,WU Yan,HUANG Cheng-bin,FU Song-bin,LI Pu,
王柏秋
,闫承慧,吴焱,黄承滨,傅松滨,李璞

遗传 , 2000,
Abstract: 为探讨肿瘤抑制基因对肺腺癌细胞生长的抑制作用。利用FuGene转染方法将P21基因的表达质粒转入一对分别具高、低转移能力的肺癌细胞系Anip973和AGZY83-a中,对P21蛋白过表达的细胞系进行了细胞生长曲线,克隆形成率,原位末端标记分析和流式细胞仪分析。P21蛋白过表达的一对细胞系细胞生长曲线斜率降低、克隆形式能力下降并出现明显的G1基因阻滞,但未检测到凋亡信号,结果表明P21基因的过表达通
Establishment and Characterization of Human Lung Carcinoma Cell Line HB-99
人肺癌细胞系HB-99的建立及其生物学特征 Establishment and Characterization of Human Lung Carcinoma Cell Line HB-99

黄昀,吴焱,杨焕杰,张临友,傅松滨,刘权章HUANG Yun,WU Yan,YANG Huan-jie,ZHANG Lin-you,FU Song-bin,LIU Quan-zhang
遗传 , 2001,
Abstract: 利用一例鳞癌手术标本通过原代培养建立了肺癌细胞系命名为HB-99,该细胞系呈单层贴壁生长,从相差显微镜和电镜分析具有细胞的多形性,细胞倍增时间为24小时,克隆形成率40%,染色体改变复杂,众数63-65,将细胞移植到裸鼠体内而生长的肿块具有与原始病人手术标本相似的组织形态,免疫组织化学分析,近100%的细胞表达角蛋白17(CK17),10%的细胞表达波形蛋白(vimentin),根据该细胞系的生物学特征提示HB099是一新建立的肺鳞癌细胞系。
13q14 Aberration is related to the Metastatic Potential of Human NSCLC
13q14断裂重排与非小细胞肺癌转移潜能关系的研究

HUANG Yun,YANG Huan-jie,JIN Yan,LI Hui-Min,FU Song-bin,
黄 昀
,杨焕杰,金 焰,李慧敏,傅松滨

遗传 , 2005,
Abstract: A large number of numerical and structural aberrations were analyzed in human tumor metastatic cells and 13q14 aberrations were frequently detected in some types of metastatic cancers. The rearrangement of 13q14 was identified previously in two lung adenocarcinoma cell lines with the same origin but different metastatic potential AGZY83-a and Anip973. BRI gene showed different expression levels in the cell lines as revealed by mRNA differential display (mRNA DD) in the two cell lines, and located in 13q14. In order to investigate the relationship between 13q14 abnormalities and tumor metastasis, a painting probe (13q) was used to hybridize three G-banded NSCLC cell lines with different metastatic potential. The major abnormality of 13q differs among different cell lines, including 13q32-33 frequent breakpoint in these three cell lines. But low metastatic potential cell lines PAa, SPC-1-A were not found breakpoint in 13q14, while 95D cell line with high metastatic potential had the common breakpoint 13q14 in two cell clones. The results suggested that the breakage at 13q14 may possibly be related to lung cancer metastasis. The affirmative relationship between 13q14 aberration and NSCLC needs further investigation.
Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines
野生型P53、P16基因协同对肺腺癌细胞系生长抑制作用的研究 Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines

YAN Cheng-hui,WANG Bai-qiu,WU Yan,FU Song-bin,LI Pu,
闫承慧
,王柏秋,吴焱,傅松滨,李璞

遗传 , 2001,
Abstract: To investigate the suppression effect of tumor suppressor genes in lung adenocarcinoma cell lines,we transferred a pair of lung adenocarcinoma cell lines with different metastasis potential,Anip973(High metastasis potential cell line) and AGZY83-a (Low metastasis potential cell line)and this pair of cell lines transfected with P16 gene:AGZY83-a P16 and Anip973 P16 with wild type P53 gene with adenovirus vector.The suppression effects of P53 gene were evaluated by cell growth curve,MTT,western blotting analysis and TUNEL technique.Overexpression of wild type P53 gene in AGZY83-a,Anip973,Anip973 P16 and AGZY83-a P16 inhibited the growth of these four kinds of lung cancer cells and induced apoptosis of the cells.The suppression effect of P53 gene in Anip973 and Anip973 P16 was higher than AGZY83-a and AGZY83-a P16 while co expression of P53 and P16 in this pair of cell lines inhibited the cells more efficiently comparing with the expression of P53 alone.Wild type P53 gene might act as a candidate gene in lung adenocarcinoma gene therapy while co transfection of P53 and P16 genes was a more effective method.
中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存 Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China
薛雅丽,王琦,史忠诚,刘岸,张钰,黄小义,黄承滨,陈白滨,杨焕杰,傅松滨,李璞XUE Ya-li,WANG Qi,SHI Zhong-cheng,LIU An,ZHANG Yu,HUANG Xiao-yi,HUANG Cheng-bin,CHEN Bai-bin,YANG Huan-jie,FU Song-bin,LI Pu
遗传 , 2001,
Abstract: 本文采用EBV(Epstein?Barr Virus)上清液转化B淋巴细胞,并加入环胞霉素A(Cyclosporine A)抑制T淋巴细胞,成功地对中国东北地区鄂温克族、鄂伦春族及达斡尔族的部分个体建立了永生细胞系,其中鄂温克族49株,鄂伦春族40株,达斡尔族51株,总计140株。永久保存我国特有民族的基因组,为分析其遗传学差异奠定了基础。 Abstract:The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhabit the activity of T cells.In the present study ,140 immortal cell lines of the Ewenki,the Oroqen and the Daur ethnic groups in the Northeast China were established .This is an important part of the research of human genome diversity for the exploration of the origin and progression of different ethnic groups ,and also provide enough research materials for further studies.
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