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Search Results: 1 - 10 of 13608 matches for " FAN Meizhen "
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Discovery and demonstration of the teleomorph ofBeauveria bassiana (Bals.) Vuill., an important entomogenous fungus
Zengzhi Li,Chunru Li,Bo Huang,Meizhen Fan
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187215
Abstract: ACordyceps specimen was collected in Anhui, China, a strain ofBeauveria bassiana, an important entomopathogenic fungus for biological pest control, was isolated and their relationship was demonstrated by microcycle conidiation. The teleomorph is an undescribed species and is namedCordyceps bassiana.
Discovery and demonstration of the teleomorph of Beauveria bassiana (Bals.) Vuill., an important entomogenous fungus

Zengzhi Li,Chunru Li,Bo Huang,Meizhen Fan,

科学通报(英文版) , 2001,
Abstract: A Cordyceps specimen was collected in Anhui, China, a strain of Beauveria bassiana, an important ento-mopathogenic fungus for biological pest control, was isolated and their relationship was demonstrated by microcycle co-nidiation. The teleomorph is an undescribed species and is named Cordyceps bassiana.

Li Zengzhi,Huang Bo,Fan Meizhen,

菌物学报 , 1997,
Abstract: 记录了最近在中国记载的侵染双翅目昆虫的虫霉目两个新种,毛蚊虫疠霉(Pandora bibionis)发现于浙江庆元的毛蚊(Bibio sp),其初生分生孢子拟卵形,多对称144~20.5×7.2~11.5μm(平均16.5~174×8.4~10.4μm), L/D 1.4~2.3(平均1.7~2.0);假根2倍粗于分生孢子梗,末端膨大为吸盘状固着器;休眠孢子外壁刺毛状,19.1~23.4μm。食蚜蝇干尸霉(Tarichium syrphis)发现于陕西镇坪县的斑翅狭口食蚜蝇上,其休眠孢子外被圆锥形长刺,长2.2~5.0μm,直径21.2~28.1μm。在毛蠓上发现中国新记录胶孢虫疠霉,并根据本文所采用的Humber(1989)系统,将其组合为胶孢虫瘴霉(Furia gloeosora comb. Nov.);此外,还将北虫疫霉(Erynia borea)组合为北虫疠霉(Pandoraborea comb. Nov.),并同时给予修订。

Hu Jinjiang Fan Meizhen Gao Xiaoman,

菌物学报 , 1994,
Abstract: Immunological studies of Metarhizium strains from different places and hosts were made by using immunoelectrophoresis and agglutination reaction. Antigens were conidium suspension and mycelium solution. Antisera were produced in rabbits from each of the 7 fungal strains. Each antigen was tested against the homo logous and heterologous antisera. The results of agglutination and the number of precipitin arcs obtained from immunoelectrophoresis were analyzed. These tests revealed numerous antigenic similarities between different strains of the same species. The grouping of strains obtained from immunoelectrophoretic data corresponds with that based on morphology.
Generation and Analysis of a Large-Scale Expressed Sequence Tag Database from a Full-Length Enriched cDNA Library of Developing Leaves of Gossypium hirsutum L
Min Lin, Deyong Lai, Chaoyou Pang, Shuli Fan, Meizhen Song, Shuxun Yu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076443
Abstract: Background Cotton (Gossypium hirsutum L.) is one of the world’s most economically-important crops. However, its entire genome has not been sequenced, and limited resources are available in GenBank for understanding the molecular mechanisms underlying leaf development and senescence. Methodology/Principal Findings In this study, 9,874 high-quality ESTs were generated from a normalized, full-length cDNA library derived from pooled RNA isolated from throughout leaf development during the plant blooming stage. After clustering and assembly of these ESTs, 5,191 unique sequences, representative 1,652 contigs and 3,539 singletons, were obtained. The average unique sequence length was 682 bp. Annotation of these unique sequences revealed that 84.4% showed significant homology to sequences in the NCBI non-redundant protein database, and 57.3% had significant hits to known proteins in the Swiss-Prot database. Comparative analysis indicated that our library added 2,400 ESTs and 991 unique sequences to those known for cotton. The unigenes were functionally characterized by gene ontology annotation. We identified 1,339 and 200 unigenes as potential leaf senescence-related genes and transcription factors, respectively. Moreover, nine genes related to leaf senescence and eleven MYB transcription factors were randomly selected for quantitative real-time PCR (qRT-PCR), which revealed that these genes were regulated differentially during senescence. The qRT-PCR for three GhYLSs revealed that these genes express express preferentially in senescent leaves. Conclusions/Significance These EST resources will provide valuable sequence information for gene expression profiling analyses and functional genomics studies to elucidate their roles, as well as for studying the mechanisms of leaf development and senescence in cotton and discovering candidate genes related to important agronomic traits of cotton. These data will also facilitate future whole-genome sequence assembly and annotation in G. hirsutum and comparative genomics among Gossypium species.
Upland Cotton Gene GhFPF1 Confers Promotion of Flowering Time and Shade-Avoidance Responses in Arabidopsis thaliana
Xiaoyan Wang, Shuli Fan, Meizhen Song, Chaoyou Pang, Hengling Wei, Jiwen Yu, Qifeng Ma, Shuxun Yu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091869
Abstract: Extensive studies on floral transition in model species have revealed a network of regulatory interactions between proteins that transduce and integrate developmental and environmental signals to promote or inhibit the transition to flowering. Previous studies indicated FLOWERING PROMOTING FACTOR 1 (FPF1) gene was involved in the promotion of flowering, but the molecular mechanism was still unclear. Here, FPF1 homologous sequences were screened from diploid Gossypium raimondii L. (D-genome, n = 13) and Gossypium arboreum L. genome (A-genome, n = 13) databases. Orthologous genes from the two species were compared, suggesting that distinctions at nucleic acid and amino acid levels were not equivalent because of codon degeneracy. Six FPF1 homologous genes were identified from the cultivated allotetraploid Gossypium hirsutum L. (AD-genome, n = 26). Analysis of relative transcripts of the six genes in different tissues revealed that this gene family displayed strong tissue-specific expression. GhFPF1, encoding a 12.0-kDa protein (Accession No: KC832319) exerted more transcripts in floral apices of short-season cotton, hinting that it could be involved in floral regulation. Significantly activated APETALA 1 and suppressed FLOWERING LOCUS C expression were induced by over-expression of GhFPF1 in the Arabidopsis Columbia-0 ecotype. In addition, transgenic Arabidopsis displayed a constitutive shade-avoiding phenotype that is characterized by long hypocotyls and petioles, reduced chlorophyll content, and early flowering. We propose that GhFPF1 may be involved in flowering time control and shade-avoidance responses.
Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Jianhui Ma, Hengling Wei, Meizhen Song, Chaoyou Pang, Ji Liu, Long Wang, Jinfa Zhang, Shuli Fan, Shuxun Yu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0049244
Abstract: Background Previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. However, only 51 anther ESTs of Upland cotton (Gossypium hirsutum) were found in NCBI and there have been no reports of transcriptome profiling analyzing anther development in Upland cotton, a major fiber crop in the word. Methodology/Principal Finding Ninety-eight hundred and ninety-six high quality ESTs were sequenced from their 3′-ends and assembled into 6,643 unigenes from a normalized, full-length anther cDNA library of Upland cotton. Combined with previous sequenced anther-related ESTs, 12,244 unigenes were generated as the reference genes for digital gene expression (DGE) analysis. The DGE was conducted on anthers that were isolated at tetrad pollen (TTP), uninucleate pollen (UNP), binucleate pollen (BNP) and mature pollen (MTP) periods along with four other tissues, i.e., roots (RO), stems (ST), leaves (LV) and embryos (EB). Through transcriptome profiling analysis, we identified 1,165 genes that were enriched at certain anther development periods, and many of them were involved in starch and sucrose metabolism, pentose and glucuronate interconversion, flavonoid biosynthesis, and ascorbate and aldarate metabolism. Conclusions/Significance We first generated a normalized, full-length cDNA library from anthers and performed transcriptome profiling analysis of anther development in Upland cotton. From these results, 10,178 anther expressed genes were identified, among which 1,165 genes were stage-enriched in anthers. And many of these stage-enriched genes were involved in some important processes regulating anther development.
Generation of ESTs for Flowering Gene Discovery and SSR Marker Development in Upland Cotton
Deyong Lai, Huaizhu Li, Shuli Fan, Meizhen Song, Chaoyou Pang, Hengling Wei, Junjie Liu, Dong Wu, Wenfang Gong, Shuxun Yu
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0028676
Abstract: Background Upland cotton, Gossypium hirsutum L., is one of the world's most important economic crops. In the absence of the entire genomic sequence, a large number of expressed sequence tag (EST) resources of upland cotton have been generated and used in several studies. However, information about the flower development of this species is rare. Methodology/Principal Findings To clarify the molecular mechanism of flower development in upland cotton, 22,915 high-quality ESTs were generated and assembled into 14,373 unique sequences consisting of 4,563 contigs and 9,810 singletons from a normalized and full-length cDNA library constructed from pooled RNA isolated from shoot apexes, squares, and flowers. Comparative analysis indicated that 5,352 unique sequences had no high-degree matches to the cotton public database. Functional annotation showed that several upland cotton homologs with flowering-related genes were identified in our library. The majority of these genes were specifically expressed in flowering-related tissues. Three GhSEP (G. hirsutum L. SEPALLATA) genes determining floral organ development were cloned, and quantitative real-time PCR (qRT-PCR) revealed that these genes were expressed preferentially in squares or flowers. Furthermore, 670 new putative microsatellites with flanking sequences sufficient for primer design were identified from the 645 unigenes. Twenty-five EST–simple sequence repeats were randomly selected for validation and transferability testing in 17 Gossypium species. Of these, 23 were identified as true-to-type simple sequence repeat loci and were highly transferable among Gossypium species. Conclusions/Significance A high-quality, normalized, full-length cDNA library with a total of 14,373 unique ESTs was generated to provide sequence information for gene discovery and marker development related to upland cotton flower development. These EST resources form a valuable foundation for gene expression profiling analysis, functional analysis of newly discovered genes, genetic linkage, and quantitative trait loci analysis.

Fan Meizhen,Hu Jinjiang,Li Nongchang,Li Li,

微生物学通报 , 1994,
Abstract: Beauveria bassiana extracellar protease was measured simply and quickly by bothgelatinagar and double-decker papilionaceous paperassays.There was a obvious linear relationshipbetween protease production by the varions strains and tleir virulence to the massonpine caterpil-lar,Dendrolimus punctatus.

Wang Chengshu,Huang Bo,Fan Meizhen,Li Zhengzhi,

微生物学通报 , 1998,
Abstract: In the present paper, the conidial count of Beauveria bassiana conidial powder determined by hemocytometer was studied. After diluted with the selected surfactant"SF1", the acquired conidial suspension was tested for absorbance at the wavelengths of 470nm with model 721 spectophotometer. The regression equation of absorbance (x) and conidial count (y) from hemocytometer counting was y=10 0.8895x 6.5869 (r=0. 9678). There was no significant difference to different strains. In measunng proceeding, the conidial suspension should be diluted by 1500-2000 fold.
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