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Search Results: 1 - 10 of 531147 matches for " F.J.C. Odibo "
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Studies and Characterization of Bacterial Spot Pathogen of Tomato Xanthomonas campestris PV Vesicatoria
E.U. Opara,F.J.C. Odibo
Journal of Molecular Genetics , 2013,
Abstract: Investigation was conducted in the laboratory to characterize, identify and determine the strains of bacteria responsible for bacterial spot disease of tomato in humid tropics of South Eastern Nigeria. The bacteria were isolated from infected tomato plant; leaf, fruit and seed and subjected to some standard bacteriological tests. The results of the cultural, physiological and biochemical analyses obtained showed that the bacteria isolated from bacterial spot lesions of tomato fruits, leaves and seed were gram negative, yellow aerobic rod shaped bacteria with a polar flagella. The bacteria colonies exhibited strong starch hydrolysis, metabolized glucose and produced acid from arabinose, sucrose and cellobiose but not from ducitol or sorbitol. Also nitrite was not reduced to nitrite based on bacteriological characteristics, the bacteria strains were identified as Xanthomonas campestris pv. vesicatoria (ex Doidge). The pathogens were found to be influenced by temperature with optimum temperature of 30-32°C but little or no growth at 20 and 45°C.
Diastatic Power and Hot Water Extract Development During Malting of Two Nigerian Millet Varieties
L.O. Eneje,F.J.C. Odibo,C.D. Nwani
Journal of Food Technology , 2013, DOI: 10.3923/jftech.2012.113.116
Abstract: Two millet varieties (P. maiwa and Sossat) of similar nitrogen contents were malted for 6 days under similar conditions to investigate the pattern of diastatic power and hot water extract development of their endosperm materials. Both parameters were found to increase as germination lasted up to the 5th day of germination, supporting high amylolytic activity of starch degradation of enzymes. A very strong correlation analysis value of 0.9444 was found between the two varieties over the same germination time. In like manner when correlation analysis for the development of hot water extract was performed for both samples over the same period, a strong correlation value of 0.9287 was obtained. Both varieties have demonstrated high potential of producing enzymes during malting making them very good candidates for producing high quality malts for beverage production.
Purification and Some Properties of Thermo-stable Alkaline Serine Protease from Thermophilic Bacillus sp. Gs-3
U.O. George-Okafor,F.J.C. Odibo
Journal of Biological Sciences , 2011,
Abstract: There are limited reliable information on the commercial production and utilization of proteases for detergent and other industrial uses in Nigeria. Hence, the purification and characterization of Bacillus sp. Gs-3 protease for its potential industrial uses were investigated. The dialyzed crude enzyme was purified 17-fold in a two-step procedure involving carboxymethyl sepharose ionic-exchange chromatography and phenyl sepharose 6-fast flow hydrophobic interaction chromatography. The purified enzyme had its optimal activity at pH 9.0 and 90C and was stable over a pH range 8.0-11.0. It readily hydrolyzed all the tested protein substrates but exhibited highest affinity for gelatin (Km 0.15 mg mL-1). It retained at least 66.20.02% of its original activity in the presence of the tested local commercial detergents and removed bloodstains completely. Its activity was significantly (p>0.05) enhanced by Cu2+ ion but strongly inhibited (75.60.07%) by Phenyl-methyl Sulfonyl Fluoride (PMSF). Thus, the enzyme demonstrated desirable properties suitable for its biotechnological applications especially in detergent industry.
Estrutura populacional do rebanho Tabapu? registrado no Brasil
Vercesi Filho, A.E.;Faria, F.J.C.;Madalena, F.E.;Josahkian, L.A.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002, DOI: 10.1590/S0102-09352002000600009
Abstract: the population structure of the registered tabapu? cattle in brazil was described. descriptive statistics of the distribution of the number of progeny, the generation interval, f-statistics, effective number of founders, ancestors, reminiscent genomes and effective population size were estimated using pedigree records from registered animals from 1971 to 1998. in the last period studied (1994-1998), 37,778 animals, 18,736 males and 19,042 females from 136 breeders were registered. the generations intervals calculated in each of the four periods 1979-1983, 1984-1988, 1989-1993 and 1994-1998 were, respectively, 6.26, 7.18, 7.68 and 7.56 yr for parent-progeny and 6.18, 6.43, 6.99 and 7.27 yr for parent to parent. the effective size of the population, founders, ancestors and reminiscent genomes in each of these periods were respectively: 378, 218, 213 and 181; 131, 254, 202 and 163; 73, 150, 119 and 94; 55, 112, 78 and 61.
Estrutura populacional da ra?a Nelore Mocho
Faria, F.J.C.;Vercesi Filho, A.E.;Madalena, F.E.;Josahkian, L.A.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002, DOI: 10.1590/S0102-09352002000500008
Abstract: the population structure of the polled nelore breed using pedigree records from animals registered from 1969 to 1998 was described. the data file was divided in the following periods: 1979-1983, 1984-1988, 1989-1993 and 1994-1998. descriptive statistics of the number of breeders, sires and dams were calculated. the total inbreeding increased from 0.55% to 0.98%, the inbreeding expected under random mating increased from 0.11% to 0.56%, and the inbreeding due to population subdivision was constantly around 0.45%, indicating that genetic subdivisions of the polled nelore breed is near to zero and quite irrelevant. the effective population size estimated from the total inbreeding was around 120. the upgrading of non-registered animals increased from 4,630 to 13,907 animals, however, this action was not enough to increase the effective population size. based on probabilities of gene origin the effective numbers of founders, ancestors and remaining genomes were calculated. these parameters decreased over the period reaching values of 144, 98 and 64. all the estimates were analogous in the description of the population genetic structure and should be considered in developing future selection programs.
Estrutura populacional do rebanho Tabapu registrado no Brasil
Vercesi Filho A.E.,Faria F.J.C.,Madalena F.E.,Josahkian L.A.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002,
Abstract: Descreveu-se a estrutura da popula o do rebanho Tabapu registrado no Brasil. Foram geradas estatísticas descritivas da distribui o do número de progênies e estimados o intervalo de gera es, estatísticas de F, número efetivo de fundadores, de ancestrais e de genomas remanescentes, além do tamanho efetivo da popula o, usando o registro genealógico de animais nascidos entre 1971-1998. Entre 1994 e 1998 foram registrados 37.778 animais, 18.736 machos e 19.042 fêmeas, pertencentes a 136 criadores. As médias dos intervalos de gera es estimadas para os períodos de 1979-1983, 1984-1988, 1989-1993 e 1994-1998 foram, respectivamente, 6,26; 7,18; 7,68 e 7,56 anos para pais-filhos e 6,18; 6,43; 6,99 e 7,27 para pais-pais. O tamanho efetivo da popula o e o número efetivo de fundadores, de ancestrais e de genomas remanescentes em cada um dos períodos foram, respectivamente, 378, 218, 213 e 181; 131, 254, 202 e 163; 73, 150, 119 e 94 e 55, 112, 78 e 61.
Estrutura populacional da ra a Nelore Mocho
Faria F.J.C.,Vercesi Filho A.E.,Madalena F.E.,Josahkian L.A.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002,
Abstract: Descreveu-se a estrutura populacional da ra a Nelore Mocho utilizando dados do registro genealógico de animais nascidos entre 1969 e 1998. O banco de dados foi separado em quatro períodos de 1979-1983, 1984-1988, 1989-1993 e 1994-1998. Calcularam-se as estatísticas descritivas do número de criadores, de reprodutores e de reprodutrizes. A endogamia total aumentou de 0,55% para 0,98%, a esperada sob acasalamento ao acaso aumentou de 0,11% para 0,56% e a endogamia devido à subdivis o populacional permaneceu constante, ao redor de 0,45%, indicando que a subdivis o genética da ra a Nelore Mocho é próxima a zero e praticamente inexistente. O tamanho efetivo populacional foi estimado pelo coeficiente total de endogamia e permaneceu ao redor de 120. A inclus o de animais no livro genealógico aumentou de 4.630 para 13.907, entretanto, esta a o n o foi suficiente para incrementar o tamanho efetivo. Baseado na probabilidade de origem do gene foram calculados o número efetivo de fundadores, de ancestrais e de genomas remanescentes. Esses parametros decresceram ao longo do período, atingindo valores de 144, 98 e 64. Todas as estimativas foram análogas na descri o da estrutura genética populacional e devem ser consideradas no futuro para o desenvolvimento de programas de sele o.
Germina??o de sementes de plantas daninhas de pastagens cultivadas: Mimosa pudica e Ipomoea asarifolia
Souza Filho, A.P.S.;Alves, S.M.;Figueiredo, F.J.C.;Dutra, S.;
Planta Daninha , 2001, DOI: 10.1590/S0100-83582001000100003
Abstract: mimosa pudica and ipomoea asarifolia are important weeds of cultivated pasture in the brazilian amazon region. this research analyzes the effects of the change in soil and climate factors on the germination (percentage and germination speed index) of these species. germination was monitored for a 15-day period, with daily counting and elimination of the germinated seeds. the results showed that soil factors such as aluminum, ph, calcium, and magnesium did not influence the germination of the two weed species. the germination of both m. pudica and i. asarifolia is independent of light exposure. optimum germination (percentage and germination speed index) temperature for both species was continuous, at 30 oc or alternated 25-35 oc temperature, even though the m. pudica seeds germinated satisfactorily in continuous temperature above and under 30 oc, and in all combinations of alternated temperatures. comparatively, the germination speed index was more sensitive to the effects of the temperature than the germination percentage. both species responded negatively to the increase in salt concentration, with m. pudica being more tolerant to salt than i. asarifolia.
Efeitos alelopáticos do calopog?nio em fun??o de sua idade e da densidade de sementes da planta receptora
Souza Filho, A.P.S.;Alves, S.M.;Figueiredo, F.J.C.;
Planta Daninha , 2003, DOI: 10.1590/S0100-83582003000200006
Abstract: the intensity of the potential allelopathic effects depends on factors either related to the donor or receiver species. this research analyzes the variations on the allelopathic effect intensities of calopo (calopogonium mucunoides) in relation to age of the donor plants (2, 4, 6, 8, 10 and 12 weeks after emergence) and to seed density of the receiver species (500, 1,000, 2,000, 3,000 and 4,000 seeds per m2). at each assigned age, plants were harvested and divided into shoot and root. the potential allelopathic effects were determined on the germination rates of the following weed seeds: mimosa pudica, urena lobata, senna obtusifolia and senna occidentalis. the intensity of the allelopathic effects was inversely related to the seed density increase. the effect was more intense for large-seeded (urena lobata and senna obtusifolia) than for small-seeded species (mimosa pudica and senna occidentalisfedegoso). age was very important for the allelopathic effects of calopo. calopo shoots showed increasing allelopathic effects with age, showing maximum effects at the age of four weeks. for roots, those effects increased until plants were 12 weeks old, when the values were higher than those observed for shoots. these results suggest that the forage legume calopo could be managed to maximize its allelopathic potential.
Effect of meconium ileus on the clinical prognosis of patients with cystic fibrosis
Oliveira, M.C.L.A.;Reis, F.J.C.;Monteiro, A.P.A.F.;Penna, F.J.;
Brazilian Journal of Medical and Biological Research , 2002, DOI: 10.1590/S0100-879X2002000100005
Abstract: the objective of the present study was to determine the possible prognostic factors which may explain the difference in the survival of patients with cystic fibrosis (cf) with and without meconium ileus. over a period of 20 years, 127 patients with cf, whose diagnosis was confirmed by typical clinical characteristics and altered sweat chloride levels, were studied retrospectively. the patients were divided into two groups: group 1 consisted of patients who presented cf and meconium ileus (n = 9), and group 2 consisted of patients with cf without meconium ileus (n = 118). the characteristics studied were based on data obtained upon admission of the patients using a specific protocol. demographic, clinical, nutritional and laboratory data were obtained. the genotype was determined in 106 patients by pcr. survival was analyzed using the kaplan-meier method. the median follow-up period was 44 months. a statistically significant difference was observed between the groups studied regarding the following variables: age at diagnosis and weight and height z scores. the presence of meconium ileus was associated with an earlier diagnosis; these patients had greater deficits in height and weight at the time of diagnosis and at the end of the study. the estimated probability of survival for patients with cf without meconium ileus was 62 ± 14% and for those with meconium ileus 32 ± 18%. patients with cf and meconium ileus presented a poor nutritional status at diagnosis and a lower survival rate compared to the general cf population.
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