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Search Results: 1 - 10 of 242165 matches for " Fábio O. Pedrosa "
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Differential metabolism of Mycoplasma species as revealed by their genomes
Arraes, Fabricio B.M.;Carvalho, Maria José A. de;Maranh？o, Andrea Q.;Brígido, Marcelo M.;Pedrosa, Fábio O.;Felipe, Maria Sueli S.;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000200004
Abstract: the annotation and comparative analyses of the genomes of mycoplasma synoviae and mycoplasma hyopneumonie, as well as of other mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. according to the annotation data, m. synoviae and m. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. both can synthesize atp by lactic fermentation, but only m. synoviae can convert acetaldehyde to acetate. also, our genome analysis revealed that m. synoviae and m. hyopneumoniae are not expected to synthesize polysaccharides, but they can take up a variety of carbohydrates via the phosphoenolpyruvate-dependent phosphotransferase system (pep-pts). our data showed that these two organisms are unable to synthesize purine and pyrimidine de novo, since they only possess the sequences which encode salvage pathway enzymes. comparative analyses of m. synoviae and m. hyopneumoniae with other mollicutes have revealed differential genes in the former two genomes coding for enzymes that participate in carbohydrate, amino acid and nucleotide metabolism and host-pathogen interaction. the identification of these metabolic pathways will provide a better understanding of the biology and pathogenicity of these organisms.
Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing
Soares-Ramos, Juliana R.L.;Ramos, Humberto J.O.;Cruz, Leonardo M.;Chubatsu, Leda S.;Pedrosa, Fábio O.;Rigo, Liu U.;Souza, Emanuel M.;
Genetics and Molecular Biology , 2003, DOI: 10.1590/S1415-47572003000400019
Abstract: herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. in this work, we analyzed six strains of h. seropedicae (z78, m2, za69, za95, z152, and z67) and one strain of h. rubrisubalbicans (m4) by restriction fragment length polymorphism (rflp) using hindiii or drai restriction endonucleases, random amplified polymorphic dna (rapd), and partial sequencing of 16s rdna. the results of these analyses ascribed the strains studied to three distinct groups: group i, consisting of m2 and m4; group ii, of za69; and group iii, of za95, z78, z67, and z152. rapd fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used. interestingly, h. seropedicae m2 was found by all analyses to be genetically very close to h. rubrisubalbicans m4. our results show that rapd can distinguish between all herbaspirillum strains tested.
Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Souza, André L.F.;Chubatsu, Leda S.;Souza, Emanuel M.;Pedrosa, Fábio O.;Monteiro, Rose A.;Rego, Fabiane G.M.;Rigo, Liu U.;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008000400022
Abstract: in prokaryotes molybdenum is taken up by a high-affinity abc-type transporter system encoded by the modabc genes. the endophyte β-proteobacterium herbaspirillum seropedicae has two modabc gene clusters and two genes encoding putative mo-dependent regulator proteins (mode1 and mode2). analysis of the amino acid sequence of the mode1 protein of h. seropedicae revealed the presence of an n-terminal domain containing a dna-binding helix-turn-helix motif (hth) and a c-terminal domain with a molybdate-binding motif. the second putative regulator protein, mode2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. we cloned the mode1 (810 bp) and mode2 (372 bp) genes and expressed them in escherichia coli as his-tagged fusion proteins, which we subsequently purified. the over-expressed recombinant his-mode1 was insoluble and was purified after solubilization with urea and then on-column refolded during affinity chromatography. the his-mode2 was expressed as a soluble protein and purified by affinity chromatography. these purified proteins were analyzed by dna band-shift assays using the moda2 promoter region as probe. our results indicate that his-mode1 and his-mode2 are able to bind to the moda2 promoter region, suggesting that both proteins may play a role in the regulation of molybdenum uptake and metabolism in h. seropedicae.
Produtividade e qualidade de genótipos de mel？o-amarelo em quatro ambientes
Araújo Neto, Sebasti？o Elviro de;Gurgel, Fábio de Lima;Pedrosa, Josué Fernandes;Ferreira, Regina Lúcia Félix;Araújo, Ant？nio de Pádua;
Revista Brasileira de Fruticultura , 2003, DOI: 10.1590/S0100-29452003000100030
Abstract: with the objective of identify genotypes of yellow melon export type and with high yield, were evaluated nine genotypes in four environments in potiguar west. the experiments were established in the mossoró-assu agricultural pole, rio grande do norte state - brazil. the experimental design was in randomized blocks with four replications and 16 plants for plot. the genotypes evaluated were: rochedo, tsx-32096; px 4910606, gold mine, px-1010606; yellow queen; gold pride; yellow king; af-646 and af-682. the genotypes that presented higher total productivity were tsx-32096, gold mine, px-4010606, yellow queen and gold pride, with average of 55.1 t/ha, and with higher marketable productivity were tsx-32096, rochedo, gold mine, px-4010606 and gold pride in mossoró (40.1 t/ha) and carnaubais (46.2 t/ha), and the genotypes af-646, yellow queen and af-682 in baraúna (63.1 t/ha), this environment provided the largest marketable productivity, overcoming in 53%, 75% and 166% the environments of carnaubais, mossoró and alto do rodrigues. the fruit mean weight was influenced by the cultivation environment, ranging from 1.07 kg in mossoró to 1.64 kg in baraúna. the higher concentration of soluble solids was verified in the genotypes af-646 (11.1%), px-4910606 (11.0%), yellow king (10.7%) and tsx-32096 (11.3%).
Produtividade e qualidade de genótipos de mel o-amarelo em quatro ambientes
Araújo Neto Sebasti？o Elviro de,Gurgel Fábio de Lima,Pedrosa Josué Fernandes,Ferreira Regina Lúcia Félix
Revista Brasileira de Fruticultura , 2003,
Abstract: Com o objetivo de identificar genótipos de mel o-amarelo, tipo exporta o, com alto rendimento, foram avaliados nove genótipos em quatro ambientes no Oeste Potiguar. Os experimentos foram conduzidos no Pólo Agrícola Mossoró-Assu, Estado do Rio Grande do Norte - Brasil. O delineamento experimental foi o de blocos casualizados, com quatro repeti es e 16 plantas por parcela. Os genótipos avaliados foram: Rochedo, TSX-32096; PX 4910606, Gold Mine, PX-1010606; Yellow Queen; Gold Pride; Yellow King; AF-646 e AF-682. Os genótipos que apresentaram maior produtividade total, foram Rochedo, TSX-32096, Gold Mine, PX-4010606, Yellow Queen e Gold Pride, com média de 55,1 t/ha, e maior produtividade comercial foram TSX-32096, Rochedo, Gold Mine, PX-4010606 e Gold Pride em Mossoró (40,1 t/ha) e Carnaubais (46,2 t/ha), e os genótipos AF-646, Yellow Queen e AF-682 em Baraúna (63,1 t/ha), ambiente que proporcionou a maior produtividade comercial, superando em 53%, 75% e 166% os ambientes de Carnaubais, Mossoró e Alto do Rodrigues. O peso médio dos frutos foi influenciado pelo ambiente de cultivo, variando de 1,07 kg em Mossoró a 1,64 kg em Baraúna. As maiores concentra es de SS foram observadas nos genótipos AF-646 (11,1%), PX-4910606 (11,0%), Yellow King (10,7%) e TSX-32096 (11,3%).
Simultaneous CXCL12 and ESR1 CpG island hypermethylation correlates with poor prognosis in sporadic breast cancer
Edneia AS Ramos, Anamaria A Camargo, Karin Braun, Renata Slowik, Iglenir J Cavalli, Enilze MSF Ribeiro, Fábio de O Pedrosa, Emanuel M de Souza, Fabrício F Costa, Giseli Klassen
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-23
Abstract: First, we analysed CXCL12 expression in breast tumour cell lines by RT-PCR. We also used 5-aza-2'-deoxycytidine (5-aza-CdR) treatment and DNA bisulphite sequencing to study the promoter methylation for a specific region of CXCL12 in breast tumour cell lines. We evaluated CXCL12 and ESR1 methylation in primary tumour samples by methylation-specific PCR (MSP). Finally, promoter hypermethylation of these genes was analysed using Fisher's exact test and correlated with clinicopathological data using the Chi square test, Kaplan-Meier survival analysis and Cox regression analysis.CXCL12 promoter hypermethylation in the first region (island 2) and second region (island 4) was correlated with lack of expression of the gene in tumour cell lines. In the primary tumours, island 2 was hypermethylated in 14.5% of the samples and island 4 was hypermethylated in 54% of the samples. The ESR1 promoter was hypermethylated in 41% of breast tumour samples. In addition, the levels of ERα protein expression diminished with increased frequency of ESR1 methylation (p < 0.0001). This study also demonstrated that CXCL12 island 4 and ESR1 methylation occur simultaneously at a high frequency (p = 0.0220).This is the first study showing a simultaneous involvement of epigenetic regulation for both CXCL12 and ESR1 genes in Brazilian women. The methylation status of both genes was significantly correlated with histologically advanced disease, the presence of metastases and death. Therefore, the methylation pattern of these genes could be used as a molecular marker for the prediction of breast cancer outcome.Breast cancer development and progression is influenced by intrinsic properties of the tumour cells, as well as by macro-environmental factors. There is an extensive interplay between tumour cells and signalling molecules such as chemokines [1,2]. Chemokine receptors and growth factors have been extensively implicated in the metastatic process of breast cancer [3]. A chemokine-mediated process
Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Monteiro, Rose A.;Schmidt, Maria A.;Baura, Valter A. de;Balsanelli, Eduardo;Wassem, Roseli;Yates, Marshall G.;Randi, Marco A.F.;Pedrosa, Fábio O.;Souza, Emanuel M. de;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008005000007
Abstract: the bacterium herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. however, the mechanisms involved in the interaction between h. seropedicae and these plants are not completely characterized. we investigated the attachment of herbaspirillum to maize roots and the invasion of the roots by this bacterium using h. seropedicae strain smr1 transformed with the suicide plasmid putkandsred, which carries a mini-tn5 transposon containing the gene for the discosoma red fluorescent protein (dsred) constitutively expressed together with the kanamycin resistance gene. integration of the mini-tn5 into the bacterial chromosome yielded the mutant h. seropedicae strain ram4 which was capable of expressing dsred and could be observed on and inside fresh maize root samples. confocal microscopy of maize roots inoculated with h. seropedicae three days after germination showed that h. seropedicae cell were attached to the root surface 30 min after inoculation, were visible in the internal tissues after twenty-four hours and in the endodermis, the central cylinder and xylem after three days.
The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae
Maria A Schmidt, Eduardo Balsanelli, Helisson Faoro, Leonardo M Cruz, Roseli Wassem, Valter A de Baura, Vinicius Weiss, Marshall G Yates, Humberto M F Madeira, Lilian Pereira-Ferrari, Maria H P Fungaro, Francine M de Paula, Luiz F P Pereira, Luiz G E Vieira, Fábio L Olivares, Fábio O Pedrosa, Emanuel M de Souza, Rose A Monteiro
BMC Microbiology , 2012, DOI: 10.1186/1471-2180-12-98
Abstract: A 21？kb DNA region of the H. rubrisubalbicans genome contains a cluster of 26 hrp/hrc genes encoding for the type three secretion system (T3SS) proteins. To investigate the contribution of T3SS to the plant-bacterial interaction process we generated mutant strains of H. rubrisubalbicans M1 carrying a Tn5 insertion in both the hrcN and hrpE genes. H. rubrisulbalbicans hrpE and hrcN mutant strains of the T3SS system failed to cause the mottled stripe disease in the sugarcane susceptible variety B-4362. These mutant strains also did not produce lesions on Vigna unguiculata leaves. Oryza sativa and Zea mays colonization experiments showed that mutations in hrpE and hrcN genes reduced the capacity of H. rubrisulbalbicans to colonize these plants, suggesting that hrpE and hrcN genes are involved in the endophytic colonization.Our results indicate that the T3SS of H. rubrisubalbicans is necessary for the development of the mottled stripe disease and endophytic colonization of rice.
Epilepsy and marijuana - a review
Dantas, Fábio Galv？o;
Journal of Epilepsy and Clinical Neurophysiology , 2005, DOI: 10.1590/S1676-26492005000200005
Abstract: abstract the medicinal use of components of cannabis sativa (marijuana) has been studied around the world. some of these components may have anti-convulsive properties, though the reports are controversial, and sometimes come from single case reports and clinical anecdotes. because of ethical aspects, as some of the components of cannabis have psychotropic effects, this is a very important issue. new researches have demonstrated that some components of cannabis that don't have psychotropic action may play a role in controlling seizures. this paper reviews the relation between epilepsy and the components of cannabis sativa.
Duas formas de se ouvir o silêncio: revisitando 4'33"
Dur？o, Fábio Akcelrud;
Kriterion: Revista de Filosofia , 2005, DOI: 10.1590/S0100-512X2005000200023
Abstract: after briefly dwelling on the relationship between art and nothing(ness), the essay interprets john cage's so-called silent piece, his much-commented 4'33'', in the context of today's semiotic overproduction. it posits a twofold way of listening to the piece, insofar as silence may be conceived either as the (impossible) aim to be reached by listening, or as a means to interrupt the continuous blabbering of things. underlying these two possibilities there are different dispositions of means and ends, namely a concentrated search for something and an aggressive passivity. these two postures are related in the text to the current state of capitalism, characterized as it is by a commodified overproduction of signs.
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