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Search Results: 1 - 10 of 591 matches for " Eveline Teixera Caixeta "
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NOTE-Polymorphic information content of SSR markers for Coffea spp.
Robson Fernando Missio,Eveline Teixera Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2010,
Abstract: Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzedin 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values werefound for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphicSSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and theleaf rust resistant arabica cultivars.
Resistência de progênies de café Catimor à ferrugem
Costa, Mauro J.N.;Zambolim, Laércio;Caixeta, Eveline T.;Pereira, Antonio A.;
Fitopatologia Brasileira , 2007, DOI: 10.1590/S0100-41582007000200005
Abstract: to study the performance of catimor genotypes, field tests were done to evaluate their productive capacity and rust incidence, while horizontal resistance of seedlings to race ii of hemileia vastatrix was tested in the greenhouse. the reaction to the pathogen was evaluated on a scale of 0 to 9 where: 0 to 3 = resistant plants and 4 to 9 = susceptible plants. the components of quantitative resistance evaluated were: infection frequency, sporulation, lesioned foliar area, incubation period and latent period. progenies ufv 5550, ufv6861, ufv 6870, ufv6831 and ufv 6834 showed similar productivity to the catuaí cultivar (p < 0.05). progenies ufv 5530, ufv 5451, ufv 5550, ufv 6903 and ufv 5464 produced no diseased plants. the descendents of the progenies in this study varied greatly for rust reaction, with continuing variation from full resistance to high susceptibility, with the majority at intermediary levels. this indicates the presence of quantitative resistance in these genotypes. compared to the catuaí cultivar, it can be concluded that the studied genotypes had quantitative resistance, with the average number of lesions and lesioned foliar area 13 to 21 times less, respectively, on ufv 6866. the sporulation of ufv 6870 reached 0.1, a very low score if compared to catuaí's score of 3.6. the incubation period of the studied genotypes varied from 18 to 36 days and the latent period from 20 to 46 days. compared to the catuaí vermelho iac 15 cultivar, it can be concluded that the great majority of catimor genotypes studied had quantitative resistance.
Heran?a da resistência do Híbrido de Timor UFV 443-03 à ferrugem-do-cafeeiro
Capucho, Alexandre Sandri;Caixeta, Eveline Teixeira;Zambolim, Eunize Maciel;Zambolim, Laércio;
Pesquisa Agropecuária Brasileira , 2009, DOI: 10.1590/S0100-204X2009000300009
Abstract: the aim of this work was to characterize the resistance inheritance of the timor hybrid ufv 443-03 to coffee leaf rust (hemileia vastatrix). for this, the race ii and pathotype 001 of coffee leaf rust were inoculated in 246 f2 plants, 115 susceptible backcrossing (bcs) plants, and 87 resistant backcrossing (bcr) plants, derived from the crossing between the susceptible genotype 'catuaí amarelo' iac 64 and the resistance source timor hybrid ufv 443-03. for both inoculums, the 'catuaí amarelo' iac 64 was susceptible, while the timor hybrid, the plant representing f1 generation, and the bcr plants were resistant. the f2 plants inoculated with race ii presented two significant segregation ratios: 15:1 and 61:3. the resistance inheritance was verified by the inoculation of the bcs plants, which segregated at a 3:1 ratio, an expected pattern for the inheritance controlled by two genes, whereas the segregation hypothesis of 7:1 for three genes was rejected. two independent and dominant genes confer the genetic resistance of timor hybrid ufv 443-03 to race ii and pathotype 001 of h. vastatrix.
Partial map of Coffea arabica L. and recovery of the recurrent parent in backcross progenies
Antonio Carlos Bai?o de Oliveira,Ney Sussumu Sakiyama,Eveline Teixeira Caixeta,Eunize Maciel Zambolim
Crop Breeding and Applied Biotechnology , 2007,
Abstract: A partial map of Coffea arabica L. was constructed based on a backcross population and RAPD markers.From a total of 178 markers evaluated, only 134 that segregated 1:1 (P>0.05) were used to develop the map. Seventeenmarkers were not linked, while 117 formed 11 linkage groups, covering a genome distance of 803.2 cM. The maximumdistance between adjacent markers was 26.9 cM, and only seven intervals exceeded 20 cM. The markers were further used forassisted selection of the plants closest to the recurrent parent, to accelerate the introgression of rust resistance genes in thecoffee breeding program. Three BC1 plants resistant to coffee leaf rust and with high genetic similarity to Catuaí wereselected and integrated in the following backcross cycles.
Development and validation of SSR markers for Coffea arabica L.
Robson Fernando Missio,Eveline Teixeira Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2009,
Abstract: With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries withprobes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences.Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were morefrequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1%of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSRmarkers were validated for further genetic studies of C. arabica.
Breeding potential and genetic diversity of "Híbrido do Timor" coffee evaluated by molecular markers
Setotaw, Tesfahun Alemu;Caixeta, Eveline Teixeira;Pena, Guilherme Ferreira;Zambolim, Eunize Maciel;Pereira, Antonio Alves;Sakiyama, Ney Sussumu;
Crop Breeding and Applied Biotechnology , 2010, DOI: 10.1590/S1984-70332010000400003
Abstract: aflp, rapd and ssr molecular markers were used to study the genetic diversity and genetic structure of the híbrido de timor germplasm. the principal coordinate analysis, upgma cluster analysis based on genetic dissimilarity of jaccard, bayesian model-based cluster analysis, percentage of polymorphic loci, shannon's information index and nei gene diversity were employed to assess the genetic diversity. the analyses demonstrated a high genetic diversity among híbrido de timor accessions. upgma and bayesian cluster analyses grouped the accessions into three clusters. the genetic structure of híbrido de timor is reported. the management of híbrido de timor germplasm variability and its potential use in breeding programs are discussed.
Contribui o ao estudo da localiza o industrial - o caso de Niterói
Marlene P.V. Teixera
Anuário do Instituto de Geociências , 1979,
In silico identification of coffee genome expressed sequences potentially associated with resistance to diseases
Alvarenga, Samuel Mazzinghy;Caixeta, Eveline Teixeira;Hufnagel, Bárbara;Thiebaut, Flávia;Maciel-Zambolim, Eunize;Zambolimand, Laércio;Sakiyama, Ney Sussumu;
Genetics and Molecular Biology , 2010, DOI: 10.1590/S1415-47572010000400031
Abstract: sequences potentially associated with coffee resistance to diseases were identified by in silico analyses using the database of the brazilian coffee genome project (bcgp). keywords corresponding to plant resistance mechanisms to pathogens identified in the literature were used as baits for data mining. expressed sequence tags (ests) related to each of these keywords were identified with tools available in the bcgp bioinformatics platform. a total of 11,300 ests were mined. these ests were clustered and formed 979 est-contigs with similarities to chitinases, kinases, cytochrome p450 and nucleotide binding site-leucine rich repeat (nbs-lrr) proteins, as well as with proteins related to disease resistance, pathogenesis, hypersensitivity response (hr) and plant defense responses to diseases. the 140 est-contigs identified through the keyword nbs-lrr were classified according to function. this classification allowed association of the predicted products of est-contigs with biological processes, including host defense and apoptosis, and with molecular functions such as nucleotide binding and signal transducer activity. fisher's exact test was used to examine the significance of differences in contig expression between libraries representing the responses to biotic stress challenges and other libraries from the bcgp. this analysis revealed seven contigs highly similar to catalase, chitinase, protein with a burp domain and unknown proteins. the involvement of these coffee proteins in plant responses to disease is discussed.
Assessment of EST-SSR markers for genetic analisys on coffee
Missio, Robson Fernando;Caixeta, Eveline Teixeira;Zambolim, Eunize Maciel;Pena, Guilherme Ferreira;Ribeiro, Ana Paula;Zambolim, Laércio;Pereira, Ant?nio Alves;Sakiyama, Ney Sussumu;
Bragantia , 2009, DOI: 10.1590/S0006-87052009000300003
Abstract: est-ssr markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. seventeen markers, developed from ests (expressed sequence tags) from the brazilian coffee genome project, were used. all markers showed polymorphism among the genotypes assessed. the average number of allele per primer was 5.1. the highest polymorphisms were found within c. canephora (88.2%) and rust-resistant varieties (35.3%). about 29.4% of the markers differentiated c. arabica from híbrido de timor; it was also possible to identify those closest and farthest from c. arabica . the analysis of population-grouped genotypes revealed a 64.0% genetic diversity among and a 36.0% genetic diversity within populations. the differentiation index was 0.637. six markers distinguished four rust-resistance varieties, showing their fingerprinting potential. these results demonstrate the usefulness of est-ssr markers for cross orientation, in diversity and introgression studies, and in genetic mapping.
Marcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem
Alvarenga, Samuel Mazzinghy;Caixeta, Eveline Teixeira;Hufnagel, Bárbara;Thiebaut, Flávia;Maciel-Zambolim, Eunize;Zambolim, Laércio;Sakiyama, Ney Sussumu;
Pesquisa Agropecuária Brasileira , 2011, DOI: 10.1590/S0100-204X2011000800015
Abstract: the objective of this work was to identify molecular markers related to the resistance of coffee (coffea arabica) to rust (hemileia vastatrix). dna sequences potentially involved in coffee disease resistance were identified, using "in silico" analysis, from data obtained by the brazilian coffee genome project. after data mining, 59 primer pairs were designed to amplify the sequences identified. the 59 primers were tested on 12 resistant and 12 susceptible coffee plants to h. vastatrix. twenty-seven primers resulted in unique and well-defined bands, while one of these amplified a dna fragment in all resistant plants, but not in the susceptible ones. this polymorphic molecular marker amplified a region of dna that corresponds to a partial open reading frame of c. arabica genome that encodes a disease resistance protein. the marker carf 005 can be used to differentiate between resistant and susceptible coffee plants to h. vastatrix.
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